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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for C Reactive Protein CRP ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were then centrifuged at 10,000 rpm for 10’ at 4°C and the supernatant was collected to determine protein concentration using Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Before loading ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Immunology 2024Quote: IFNγ in supernatants from the whole blood RSA was determined by ELISA according to the manufacturer’s instructions (Human IFNγ Uncoated ELISA kit; Invitrogen). 50μl of supernatant was diluted with 50μl of assay diluent for use in the IFNγ ELISA ...
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bioRxiv - Biochemistry 2022Quote: ... The viral RNA from patient samples were reverse transcribed with the primer DENV-CRP with the Superscript IV reverse transcriptase according to manufacturer’s instruction (Invitrogen). All template nucleic acids were quantified using Qubit-4 fluorometer when not mentioned otherwise.
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bioRxiv - Microbiology 2020Quote: ... coated to 96-Well Maxisorp® microtitre ELISA plates (Nunc, 0.1 µg BSM per well), serving as a ligand ...
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bioRxiv - Bioengineering 2022Quote: ELISA binding assays were performed using 96 well half-area plates (Fisher Scientific, Costar 3690) coated with 0.5 μg/mL of S protein and incubated overnight at 4°C ...
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bioRxiv - Bioengineering 2021Quote: ... enzyme-linked immunosorbent assay (ELISA) Nunc MaxiSorp™ flat-bottom plates (Invitrogen 44-2404-21) were coated with 2.5 μg/mL with the mixture of four antigens in phosphate coating buffer (0.1 M Na2HPO4 in deionized water ...
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bioRxiv - Immunology 2022Quote: ... The mix was added for 30 min to ELISA 384-well plates (NUNC, P6366-1CS) pre-coated overnight at 4°C with 4 μg/ml human ACE2 (produced in house ...
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bioRxiv - Immunology 2023Quote: ... The mix was added for 30 min to ELISA 384-well plates (NUNC, P6366-1CS) pre-coated overnight at 4°C with 4 µg/ml human ACE2 (produced in house ...
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bioRxiv - Immunology 2023Quote: ... 10 μgs of protein were loaded into manually coated IL-22 96-well High Affinity plates and IL-22 levels were determined by IL-22 ELISA kit (ThermoFisher-Scientific #88-7422-22) following manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2023Quote: The Nunc MaxiSorp™ C-shaped Plates (Thermo Fisher) were coated with 2.5 µg/ml of DrSA/DiSA in 0.1 M sodium carbonate buffer (pH 9.5 ...
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bioRxiv - Immunology 2023Quote: ... Total IgE concentrations were determined in previously frozen samples using a commercially available IgE ELISA kit (IgE Mouse uncoated ELISA Kit, Invitrogen-Thermo Fischer Scientific, Waltham, MA) following the manufacturer’ s instructions ...
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bioRxiv - Microbiology 2021Quote: ... 1 µl was used for protein quantification with the Pierce BCA Protein Assay Kit (Thermofisher). The remaining 9 µl were mixed with SDS (0.2% final) ...
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bioRxiv - Genetics 2023Quote: ... We incubated the cells (1 million cells / 1 ml HBSS) obtained after digestions with 1 µl Near-IR fluorescent reactive dyeLIVE/DEAD Fixable Dead Cell Stain Kit (Invitrogen; Cat: L34961; Ex: 633/635nm; Em: 775nm) for 30 min on ice and stopped the reaction by addition of 1 ml HBSS supplemented with 5% FBS ...
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bioRxiv - Microbiology 2023Quote: ... Formalin-killed N61 in PBS was dried in an oven at 5×106 cells/well in 96-well ELISA plates (C96 Maxisorpcert, Nunc-Immuno Plate, Thermo Fisher Scientific) overnight at 37°C ...
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bioRxiv - Bioengineering 2022Quote: Epidermal growth factor receptor (EGFR) protein expression levels on the surface of Gli36-derived EVs were quantified using an EGFR Human ELISA kit (ThermoFisher Scientific, Waltham, MA). EVs were spiked in healthy donor serum at different concentrations ranging from 0 to 1.0E11 particles/mL while maintaining the serum-derived EV concentration at 1.0E9 particles/mL ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-Step Turbo TMB-ELISA substrate solution (Thermo Scientific) was used for detection ...
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bioRxiv - Cell Biology 2022Quote: ... 1-Step Ultra TMB ELISA substrate (Thermo Fisher Scientific) was added ...
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bioRxiv - Physiology 2020Quote: INS-1 insulin secretion was monitored by ELISA (ThermoFisher). Cells were plated at 200k cells per 24 well ...
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bioRxiv - Cancer Biology 2021Quote: ... 1-Step Ultra TMB-ELISA solution (#34028, Thermo Fisher) was added to develop the peroxidase signal ...
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bioRxiv - Physiology 2022Quote: ... 1-Step Ultra TMB ELISA Substrate (Thermo Fisher Scientific) was added to each well ...
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bioRxiv - Neuroscience 2022Quote: ... embryoid bodies were embedded in 15 µL Matrigel droplets and cultured in 6 well plates containing c-organoid differentiation media consisting of 1:1 DMEM-F12 and Neurobasal media (Gibco), with addition of 0.5% N2 supplement (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... IL-1β secretion from PMA-THP1 was detected using IL-1 beta Human ELISA kit (#KHC0011, ThermoFisher Scientific) and IL-1 beta Human ELISA kit ...
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bioRxiv - Genomics 2020Quote: ... SDS) for 10 minutes at 95°C and protein concentration was determined using the Pierce™ BCA Protein Assay Kit (ThermoFisher Scientific 23227). Protein samples were separated by SDS-PAGE using 4-20% Mini-PROTEAN® TGX™ gels (BioRad ...
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bioRxiv - Genomics 2022Quote: ... for 30 minutes at 4°C and supernatant was retained and protein concentration quantified using the Pierce BCA protein assay kit (Thermo Fisher Scientific, 23227). A total of 25-35μg proteins were loaded onto 12.5% Tris-HCl gel for SDS-PAGE analysis ...
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bioRxiv - Cell Biology 2022Quote: ... The lysates were cleared by centrifugation at 18,000 x g and 4°C for 20 min and protein concentration was determined using the BCA Protein Assay Kit from Pierce™ (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... Plates were then washed 3 times with WB before adding 100 µL 1-Step™ Ultra TMB-ELISA Substrate Solution (Thermo Fisher 34029) followed by incubation in the dark at room temperature with shaking (225 rpm ...
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bioRxiv - Bioengineering 2022Quote: ... Plates were finally washed three more times with PBS-T and developed using 1-Step Ultra TMB-ELISA solution (Thermo Fisher Scientific #34028). The HRP reaction was stopped with 1M hydrochloric acid and absorbance values at 450 nm were measured on a SpectraMax i3x plate reader (Molecular Devices).
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bioRxiv - Microbiology 2021Quote: ... After 4x washing with PBS-T the ELISA was developed with 1-step Ultra TMB ELISA (Thermo Scientific). For generation of an oxMIF standard curve ...
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bioRxiv - Neuroscience 2022Quote: ... Serum TNF and IL-1β concentrations were determined by using a rat TNF alpha Uncoated ELISA and a rat IL-1β ELISA Kit (all from Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... Enzyme-linked immunosorbent assays (ELISAs) for human IL-8 were performed using the Human IL-8 ELISA Kit (ThermoFisher SCIENTIFIC) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... Cytokine measurement on tissue homogenate was assessed using ELISA kits according to the manufacturer instructions (Mouse IFN gamma Uncoated ELISA, Invitrogen, #88-7314 ...
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bioRxiv - Immunology 2019Quote: ... Cytokine measurement on tissue homogenate was assessed using ELISA kits according to the manufacturer instructions (Mouse IFN gamma Uncoated ELISA, Invitrogen, #88-7314; Mouse TNF alpha Uncoated ELISA, Invitrogen, #88-7324 ...
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bioRxiv - Immunology 2021Quote: IL-17a ELISAs were performed using Human IL-17A (homodimer) ELISA Ready-SET-Go!™ Kit (Invitrogen™; eBioscience™), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Reactive bands were visualized using SuperSignal West Pico (Thermo Fisher Scientific). Alternatively ...
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bioRxiv - Cancer Biology 2019Quote: Reactive oxygen species were measured using the CellROX green reagent (Thermofisher) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Alexa Fluor 555 or Alexa Fluor 647 reactive dyes (Life Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Flies were covered in yellow dust (Reactive Yellow 86, Fisher Scientific)15 and placed in a cylindrical custom designed chamber ...
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bioRxiv - Biochemistry 2022Quote: ... mono-reactive NHS ester and NHS-ester conjugated Atto488 (Thermo Fisher) were used ...
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Loss of TREM2 reduces hyperactivation of progranulin deficient microglia but not lysosomal pathologybioRxiv - Neuroscience 2021Quote: Myelin was labeled with amine reactive pHrodoTM Red succinimidyl ester (ThermoFisher) for 45 min at RT (protected from light) ...
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bioRxiv - Biophysics 2021Quote: Thiol-reactive Tb3+ chelate DTPA-cs124-EMPH (Lanthascreen, Life Technologies, Inc.) was used as the LRET donor for all samples and Bodipy FL maleimide (Invitrogen ...
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bioRxiv - Immunology 2021Quote: ... and ArC Amine Reactive Compensation Beads (Cat. No. A10346, Life Technologies) were used for compensation ...
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bioRxiv - Biochemistry 2021Quote: ... Reactive oxygen species were detected by including H2DCF-DA (ThermoFisher #D399) at a final concentration of 10 μM during the 24-h incubation ...
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bioRxiv - Neuroscience 2021Quote: ... TMT10-plex amine reactive reagents (Thermo Fisher, 5 mg per vial) were re-suspended in 1024 μL anhydrous acetonitrile and 25 μL of reagent was added to each sample (TMT label ...
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bioRxiv - Immunology 2021Quote: ... mRNA from reactive clones was extracted with TRIzol™ Reagent (Invitrogen) and transcribed to complementary DNA with Affinity Script Multiple Temperature cDNA Synthesis Kit (Agilent Technologies).
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bioRxiv - Immunology 2020Quote: ... Reactive oxygen species was measured by adding 5μM MitoSox (ThermoFisher Scientific) to the cell cultures ...