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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Cells were then harvested and stained with a amine-reactive dye (NIR, Invitrogen) and surface antibodies against CD3 ...
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bioRxiv - Immunology 2019Quote: ... either heat-killed cells or the ArC Amine Reactive Compensation Bead Kit (Invitrogen) were used ...
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bioRxiv - Physiology 2020Quote: ... Reactive astrocytes were stained using rabbit anti-Gfap antibodies (ThermoFisher, Cat# OPA1-06100). Myelin was identified using rat anti-Mbp antibodies (Abcam ...
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bioRxiv - Cell Biology 2022Quote: Total Reactive Oxygen Species (ROS) Assay Kit 520 nm (Invitrogen, Cat: 88-5930) was used to identify ROS in the cells of vascular organoids by Flow cytometry in the FITC channel as well as Confocal imaging ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were stained with Live/Dead violent fluorescent reactive dye (ThermoFisher), Fc receptor block ...
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bioRxiv - Microbiology 2023Quote: ... cells were stained with an amine reactive live/dead dye (efluor506, Thermo Scientific) and an antibody panel (Ly6G-PE ...
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bioRxiv - Cell Biology 2024Quote: ... Immunofluorescence (IF) staining was performed with antibodies for prosurfactant protein-C (proSP-C) (Invitrogen, PA5-71680), NKX2.1 (Invitrogen ...
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bioRxiv - Molecular Biology 2021Quote: ... Proteins were transferred to a nitrocellulose Hybond-C membrane (Invitrogen). The membrane was blocked in PBS-T milk 5% and probed by using anti-Flag (Sigma F1804 ...
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bioRxiv - Biochemistry 2020Quote: ... Identification and quantification of peptides and proteins were carried out in Proteome Discoverer 2.4 (Thermo Fisher) using a SwissProt human database (Tax ID 9606 ...
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bioRxiv - Molecular Biology 2023Quote: ... Robotic purification and digestion of proteins into peptides was performed on the Kingfisher Flex (ThermoFisher Scientific) using trypsin and the R2-P1 method [48] ...
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bioRxiv - Biochemistry 2023Quote: ... the peptide concentration in samples was estimated using a Micro BCATM Protein Assay (Thermo Scientific, #23235), and a volume corresponding to 25 μg peptides was transferred to a new low-bind Eppendorf tube per sample ...
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bioRxiv - Cell Biology 2023Quote: Immunoprecipitated protein complex beads were digested into peptides using 500ng sequencing grade trypsin (Thermo Fisher Scientific) and incubated overnight at 37°C on a shaker ...
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bioRxiv - Biochemistry 2024Quote: Peptide identification and TMT-based protein quantification was performed in Proteome Discoverer 2.4 (PD, Thermo Fisher). MS/MS spectra were searched using SEQUEST-HT against a Uniprot human proteome database (released 03/2014 and containing 20,337 entries ...
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bioRxiv - Microbiology 2023Quote: ... Peptide concentrations were measured using the Invitrogen Qubit protein assay kit (Thermo Fisher Scientific, Darmstadt, Germany).
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bioRxiv - Microbiology 2021Quote: ... and blocking in SuperBlock Blocking Buffer (Thermo Fisher Scientific, Waltham, MA). Cleaved caspase-3 was immunostained by incubating HAE with a primary human cleaved caspase-3 (Asp175 ...
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bioRxiv - Cancer Biology 2019Quote: ... After blocking with SuperBlock T20 (PBS) Blocking Buffer (Thermo Fisher Scientific), the plates were incubated with culture supernatant with subsequent 1:2000 diluted peroxidase-conjugated anti-mouse IgG (Agilent Technologies) ...
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bioRxiv - Neuroscience 2023Quote: ... before blocking in SuperBlock™ blocking buffer (Thermo Fisher Scientific, 37515). Primary antibodies (MAP2 (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... followed with endo-biotin blocking using a blocking kit (Invitrogen, #E21390) according to the manufacture instructions [118] ...
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bioRxiv - Microbiology 2023Quote: ... blocking was performed overnight using SEA BLOCK Blocking buffer (ThermoFisher, 37527) at 4°C ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... 4 °C) and the total protein concentration determined by BCA protein assay (Pierce BCA Protein Assay Kit, Thermo Scientific, 23225) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2022Quote: ... The cells were blocked with 10% Pierce™ Protein-Free (PBS) Blocking Buffer (#37572; Thermo Fisher Scientific) in PBS for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... followed by destaining using 25 mM NaOH and blocking using 1x BlockerTM FL Fluorescent protein block (ThermoFisher). Primary antibodies used were Anti-GapDH (G9545 ...
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bioRxiv - Biochemistry 2020Quote: ... The protein with the C-terminal Fc-fusion was affinity purified with protein-A sepharose (Invitrogen). Samples were eluted with 0.1 M glycine pH 3.0 directly to a neutralizing buffer ...
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bioRxiv - Developmental Biology 2021Quote: ... All the digested peptides were labelled withiTRAQ 4-plex and purified using C-18 spin columns (Thermo Scientific). Labelled and purified peptides were analysed using LC-MS/MS OrbitrapVelos Nano analyser (Thermo Scientific ...
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bioRxiv - Cancer Biology 2019Quote: ... Tryptic peptides were separated prior to analysis using a Dionex Ultimate 3000 RSL C nanosystem (Thermo Fisher Scientific) with a C18 PepMap RSLC column (75 μm × 50 cm) ...
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bioRxiv - Microbiology 2021Quote: ... The peptides were separated on a Thermo EASY-Spray column (Thermo Scientific 50cm column, column temperature 45 °C) operated at a maximum pressure of 800 bar ...
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bioRxiv - Microbiology 2022Quote: ... was synthesized as a custom peptide with N-terminal acetylation and C-terminal amidation (Life Technologies Europe Bv).
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bioRxiv - Cell Biology 2024Quote: ... The peptide digests were frozen at −80°C and dried by vacuum centrifugation (Savant SPD121P, Thermo Fisher Scientific), reconstituted in 0.07% trifluoroacetic acid ...
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bioRxiv - Microbiology 2023Quote: ... The peptide concentrations of eluted peptides were quantified using the Pierce Fluorometric Peptide Assay (Thermo Fisher Scientific) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: ... Samples were then washed with 1xPBS and blocked for 30 minutes at 37°C in blocking buffer (1xPBS, 0.05% BSA [Invitrogen, Am2616] ...
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bioRxiv - Immunology 2021Quote: ... Blots were incubated overnight at 4°C with agitation in blocking buffer and anti-HisG-HRP (1:5000, Invitrogen), or anti-V5-HRP (1:5000 ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were quantified with Quantitative Colorimetric Peptide Assay (Thermo Scientific) and 5 µg of total peptides were labeled using TMTpro16-plex reagents (Thermo Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... Peptides were desalted with peptide desalting spin columns (Thermo Scientific) and dried via vacuum centrifugation ...
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bioRxiv - Molecular Biology 2021Quote: ... and reactive bands were visualized using Pierce ECL Western Blotting Substrate (Thermo Scientific #32109). Western blots images were collected on a BioRad ChemiDoc XRS+ using the Image Lab software (BioRad).
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bioRxiv - Immunology 2021Quote: T-cells were viability stained with Live Dead Near IR amine reactive dye (ThermoFisher), fixed/permeabilized and antibody stained per the protocol included in the supplemental materials ...
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bioRxiv - Biochemistry 2020Quote: Reactive oxygen species levels were estimated using the probe CM-H2DCFDA (Molecular probes, Invitrogen). NSCs (SCZ and CTRL ...
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bioRxiv - Biochemistry 2020Quote: Reactive oxygen species levels were estimated using the probe CM-H2DCFDA (Molecular probes, Invitrogen). NSCs (SCZ and CTRL ...
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bioRxiv - Microbiology 2020Quote: ... Reactive bands were visualized using the Super Signal West Pico Chemiluminescent substrate (ThermoFisher Scientific) and an Intas ChemoCam system (Intas Science Imaging Instruments GmbH ...
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bioRxiv - Bioengineering 2022Quote: ... we used Tetra-PEG-SH fluorescently labeled with a thiol-reactive probe (Thermo Fisher Scientific or Merck KGaA ...
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bioRxiv - Immunology 2022Quote: ... ROS was assayed using Total Reactive Oxygen Species (ROS) Assay Kit 520 nm (Invitrogen). For pDok cells were fixed with BD PhosFlow Lyse/Fix buffer then Foxp3/Transcription Factor Buffer Staining Set (eBioscience ...
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bioRxiv - Neuroscience 2021Quote: ... Synaptosomes were conjugated with an amine-reactive dye (pHrodo Red, SE; Thermo Scientific; #P36600) in 0.1 M sodium carbonate (pH 9.0 ...
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bioRxiv - Bioengineering 2021Quote: ... AbC total antibody and amine reactive ArC compensation beads kits (ThermoFisher, A10497 and A10346) were included for single stain controls ...
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bioRxiv - Immunology 2023Quote: ... and immune-reactive bands were detected with ECL Western Blotting Substrate (Thermo Fisher Scientific). Protein expression was quantified with ImageJ software (National Institutes of Health ...
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bioRxiv - Developmental Biology 2022Quote: Intracellular ROS was detected using a Reactive Oxygen Species Assay Kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... A 40-minute blocking step with Super Block Blocking buffer (Thermo Scientific) was performed prior to adding the primary antibody ...
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bioRxiv - Biochemistry 2020Quote: ... Concentration of the recombinant peptides was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, USA) with bovine serum albumin as the standard.
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bioRxiv - Biochemistry 2021Quote: Hela cell tryptic peptides were obtained from the commercially prepared Pierce HeLa Protein Digest Standard (Thermo Fisher). Each vial was reconstituted in 2% acetonitrile (ACN)/1% formic acid (FA ...
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bioRxiv - Plant Biology 2023Quote: ... Eluates were combined and peptide content was estimated using protein A280 on a NanoDrop 2000 (Thermo Scientific). Eluate was split into two fractions with the smaller fraction (∼5µg of peptide ...
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bioRxiv - Biophysics 2023Quote: ... The sample was purified using High Protein and Peptide Recovery Detergent Removal Spin Column (Thermo Fisher Scientific) and Pierce peptide desalting spin columns (Thermo Fisher Scientific ...