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Citations for Thermo Fisher :
201 - 250 of 10000+ citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... 1% P/S (ThermoFisher Waltham, MA), 10 ng/mL rhGM-CSF (R&D Systems Minneapolis ...
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bioRxiv - Cell Biology 2024Quote: ... antibiotics (P/S; Gibco, 15070, 063) and trypsin (Gibco,15400 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% penicillin/streptomycin (P/S; Invitrogen) in a humidified 5% CO2 incubator at 37°C ...
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bioRxiv - Genetics 2020Quote: ... This mixture was incubated overnight at 4°C with beads (Protein A Dynabeads, Invitrogen, for CTCF ChIP ...
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bioRxiv - Plant Biology 2021Quote: ... The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher) and washed three times with 100 μl IP buffer to isolate homogeneous SEP3-AGIAP1complex ...
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bioRxiv - Microbiology 2022Quote: ... and c-Myc-tagged N protein together with GFP using Lipofectamine 2000 (Life Technologies), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: ... Chromatin was incubated overnight at 4°C with magnetic beads (Dynabeads Protein G, Invitrogen) and the following antibodies (4 µg each) ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were digested with 12 ng/mL Trypsin/Lys-C (Thermo Fisher Scientific # A41007) overnight at 37 °C ...
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bioRxiv - Microbiology 2023Quote: ... Specific epithelial cell proteins were detected by immunoblotting with anti-c-Met (#370100, Invitrogen), anti-EGFR (#2234 ...
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bioRxiv - Biochemistry 2023Quote: Melting temperature (°C) was determined by adding SYPRO™ Orange protein stain (Thermo Fisher) to 5 μM Fab protein in PBS and performing a thermal melt of 25-95 °C (0.5 °C /30 sec intervals) ...
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bioRxiv - Molecular Biology 2023Quote: ... Immunoprecipitations were performed overnight at 4°C with protein G Dynabeads (Thermo Fisher Scientific) coupled to either rabbit immunoglobulin G (IgG ...
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bioRxiv - Immunology 2023Quote: ... were coated overnight at 4°C with 2 µg/ml CD40 protein (ThermoFisher, A42565) in fresh PBS ...
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bioRxiv - Microbiology 2023Quote: ... and then rotated overnight at 4°C with 1.5 mg protein G (Dynabead, Invitrogen). Complexes were precipitated by centrifugation and washed three times in PBS supplemented 0.02% Tween 20 and resuspended in 100 uL PBS prior to elution with 40 uL of 2 parts 0.1M glycine pH 2.8 and 1 part NuPage sample buffer and reducing agent (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... The proteins were purified via CaptureSelect™ C-tag affinity matrix (Thermo Fisher Scientific). A further SEC polishing step was performed on a HiLoad 16/600 Superdex 200 pg column (GE Healthcare ...
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Sapogenin based self-assembly structures activating a non-apoptotic cell death via multiple pathwaysbioRxiv - Pharmacology and Toxicology 2021Quote: 100 mg AG was dissolved in pyridine and 450 mg p-TsCl (p-Tosyl Chloride, Acros Organics) reagent was added ...
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bioRxiv - Developmental Biology 2022Quote: ... The following primary antibodies were used: rabbit anti-p-MLC2 (p-Ser20; Fisher Scientific, NC0279729; 1:100) rabbit anti-Vimentin (Santa Cruz ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used: mouse monoclonal antibody against phosphorylated microtubule-associated protein tau (p-tau, UniProtKB P10637. Thermo Fisher Scientific, MN1020), mouse monoclonal antibody against slow myosin heavy chain I (MYH1 ...
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bioRxiv - Microbiology 2021Quote: ... The gene of VSV P protein were amplified and synthesized in vitro using mMESSAGE mMACHINE® Kit (Life technologies, Cat. No. AM1344) to serve as a standard ...
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bioRxiv - Cell Biology 2023Quote: ... and Alt-R® CRISPR-Cas9 tracrRNA Atto550 labeled, IDT) and recombinant Cas9 protein (Alt-R® S. p. HiFi Cas9 Nuclease V3, IDT) using Lipofectamine™ CRISPRMAX (Invitrogen) and cultured for 48 h in the presence of an HDR enhancer (final concentration 20 μM ...
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bioRxiv - Cancer Biology 2023Quote: ... and the total protein concentration was determined using a Pierce Reducing Agent Compatible BCA kit (RAC-BCA, Thermo Scientific, P/N 23252) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... supernatants were stored at - 80°C and protein concentrations were further determined using the Pierce™ BCA Protein Assay Kit (23225, ThermoFisher Scientific).
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bioRxiv - Microbiology 2019Quote: ... Samples were centrifuged at 10,000 x g for 30 min at 4 °C before supernatants were collected and protein content was determined using the BCA Protein Assay Kit (Pierce, Thermo Fisher Scientific) following the manufacturer’s instructions.
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bioRxiv - Physiology 2020Quote: ... and MOTS-c in each group was normalized to the total tissue protein concentration as determined by the Bradford protein assay (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2019Quote: ... The lysate was centrifuged at (21000 g) for 15 min at 4°C and protein concentration determined (Pierce™ 660nm Protein Assay Reagent, Thermo Scientific). 8.5 μg lysate was kept for western blot analysis and 1.6-1.8 mg total protein was added to 250 μl washed high capacity neutravidin beads (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were centrifuged at 14000 rpm for 15 minutes at 4°C and protein concentration determined with a Pierce™ BCA Protein Assay Kit (Life Technologies). High capacity neutravidin beads (Life Technologies ...
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bioRxiv - Systems Biology 2020Quote: ... Lysates were clarified by centrifugation at 10,000 rpm for 2 minutes at 4°C and snap frozen in liquid nitrogen with protein concentrations measured using the BCA protein assay (Pierce/Thermo Fisher Scientific). For RAS activation assays ...
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bioRxiv - Neuroscience 2022Quote: ... Proteins were denatured by boiling at 95°C for 10 mins and subjected to a PierceTM BCA protein assay (Life Technologies, 23227) for determination of the protein concentration ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Microbiology 2020Quote: ... Lysates were clarified by centrifugation (12,000 g for 30 min at 4 °C) and protein concentrations were determined using a BCA protein assay kit (Thermo Fisher Scientific). SOD activity in whole cell extracts was measured using the EnzyChrome superoxide dismutase assay kit (BioAssay Systems ...
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bioRxiv - Cell Biology 2020Quote: ... Cell debris were removed by centrifugation at 1000 × g for 5 min at 4°C and the protein concentration of the resulting supernatant was estimated using the BCA protein assay kit (Thermo Fisher Scientific) with bovine serum albumin as the standard ...
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bioRxiv - Genomics 2022Quote: ... 10 min at 4°C and supernatants quantified for protein content using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific, 23227) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... Samples were cleared by centrifugation at 4°C and 23,000×g for 30 min and protein concentration determined using Pierce(tm) BCA Protein Assay (Thermo Fisher Scientific, USA). 0.2 μg/μl α-casein were added to a protein amount of 1.2 mg ...
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bioRxiv - Developmental Biology 2023Quote: ... Whole cell lysates were centrifuged for 15 min at maximum speed at 4°C and protein concentrations measured from the supernatant with a Pierce BCA Protein Assay Kit (Thermo Scientific, 23227). 15 µg protein per lane were resolved on 8% SDS-PAGE ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifuging at 12.000 rpm for 5 min at 4°C and protein concentration determined using the Pierce™ BCA Protein Assay (Thermo Scientific 23225). Samples were prepared for SDS-Page in 1× NuPAGE™ LDS Sample Buffer containing 50mM DTT ...
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bioRxiv - Neuroscience 2023Quote: Samples (input (eluted in 2× SDS buffer and thawed 15 minutes at 75°C) and IP proteins) were run in NuPAGE 4%–12% Bis-Tris Mini Protein Gel (10 wells) (ThermoFisher Scientific, NP0321BOX) at the voltage of 100V for 2.5 hours in a NuPAGE MOPS SDS Running buffer (ThermoFisher ...
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bioRxiv - Biochemistry 2023Quote: ... The lysates were then centrifuged for 15 min at 16,000 × g at 4°C and their protein concentrations were determined using a BCA Protein Assay Kit (#23227, Thermo Fisher Scientific, USA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Lysates were then centrifuged at 10,000 rpm for 10’ at 4°C and the supernatant was collected to determine protein concentration using Pierce BCA Protein Assay Kit (#23225, Thermo Fisher Scientific). Before loading ...
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bioRxiv - Plant Biology 2023Quote: ... The lysate was cleared by centrifugation (18,000 x g for 10 min at 25°C) and the protein content of the supernatant was determined using the Pierce BCA protein assay kit (Thermo Fisher Scientific). Reduction ...
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bioRxiv - Cell Biology 2023Quote: Cells were lysed in 1x RIPA Buffer pre-warmed to 95°C and the total protein concentration was normalised using the Pierce BCA Protein Assay (Cat. # 23227; Thermo Fisher Scientific). Proteins (20 µg ...
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bioRxiv - Biochemistry 2021Quote: ... 4°C and the protein was purified from the supernatant using Ni-NTA Agarose (Invitrogen). The beads were washed with His-SUMO buffer and protein was eluted with His-SUMO elution buffer (50 mM Tris-HCl pH 7.5 ...
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bioRxiv - Plant Biology 2021Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein A Dynabeads (Invitrogen) precoated with the corresponding antibodies ...
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bioRxiv - Cell Biology 2021Quote: ... endogenous c-MET was immunoprecipitated on MET antibody-bound beads (Protein G from Thermo Fisher) and Flag-tagged HGF WT and HGF 4Cys-4Ala were in-vitro translated (TNT quick coupled Transcription/Translation system ...
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bioRxiv - Immunology 2020Quote: ... 000 x g at 4°C and protein concentration measured using Pierce BCA (Thermo Fisher). Equivalent amount of protein in each sample were immunoprecipitated by rotating with 0.25-1 µg of FADD antibody (M19 ...
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bioRxiv - Immunology 2022Quote: All experiments were performed at 4°C using ProtoArray Human Protein Microarrays (Thermo Fisher Scientific). Microarrays were blocked for 1 h in blocking solution (Thermo Fisher) ...
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bioRxiv - Neuroscience 2019Quote: ... 500 µg of proteins were used for c-Myc-tagged tomosyn co-immunoprecipitation (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2020Quote: ... For IP of hnRNP-C and RALY 50 μl of Protein G Dynabeads (Thermo Fisher) per sample were washed 3x in IP buffer (50 mM HEPES pH 7.4 ...
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bioRxiv - Plant Biology 2023Quote: ... The eluted protein was then immobilized on anti-c-Myc magnetic beads (Thermo Fisher 88843) and washed three times with 100 μl IP buffer to isolate homogeneous SEP3Δtet3M-AG or SEP3Δtet-AGΔtet complexes ...
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bioRxiv - Plant Biology 2023Quote: ... Chromatin samples were incubated for 12 h at 4 °C with Protein G Dynabeads (Invitrogen) precoated with the antibodies anti-FLAG (Abcam ab18230 ...
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bioRxiv - Molecular Biology 2023Quote: ... protein samples were denatured at 95°C using NuPAGE LDS sample buffer (Thermo Fisher Scientific) and separated by 4-12% sodium dodecyl sulfate-polyacrylamide gel electrophoresis ...
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bioRxiv - Immunology 2023Quote: ... the recombinant proteins were purified using a CaptureSelectTM C-tagXL Affinity Matrix prepacked column (ThermoFisher), followed the manufacturer’s guidelines as previously detailed (56 ...