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Citations for Thermo Fisher :
501 - 527 of 527 citations for Bordetella Pertussis Toxin Glycerol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Zoology 2024Quote: ... Tissue samples were subsequently rinsed three times with DPBS again and then transferred onto microscope slides in mounting buffer containing 1:1 DPBS: glycerol and 1µg/mL 4’,6-diamidino-2-phenylindole dihydrochloride (DAPI) (Molecular Probes, Eugene, OR) to stain cell nuclei in prepared tissues and examined under a Lumen Dynamics XCite™ 120Q Nikon fluorescence microscope (Nikon ...
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bioRxiv - Neuroscience 2023Quote: ... slides were first saturated with 5 mL of ddH2O on a leveled surface and gradually replaced with 80% glycerol solution containing 5% RNase inhibitor (AM2684, Thermo Fisher Scientific) through steady vacuuming off ddH2O from one end of the slide and adding glycerol with a pipette at the opposite end simultaneously ...
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bioRxiv - Physiology 2024Quote: ... The guts were mounted in 50% glycerol and visualized with the EVOS FL Auto live-cell imaging system (Life Technologies, Burlington, ON).
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bioRxiv - Systems Biology 2024Quote: ... Cells were washed with 2x SSC and then switch to a photo-protective buffer containing 50% glycerol (Thermo Fisher Scientific, Waltham, MA), 75 μg/mL glucose oxidase (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2024Quote: ... incubated for 7 min at 37°C with proteinase K in PBST (1/2000 from a glycerol stock at 20mg/ml, Ambion, Cat#10259184), washed in PBST for 2×10 min at RT ...
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bioRxiv - Genetics 2021Quote: ... Dissected brains and ovaries were rinsed with cold PBS and placed immediately in cold 8M urea lysis buffer (8M urea, 10% glycerol, 0.5% SDS, 5% β-mercaptoethanol) with Halt™ Protease Inhibitor Cocktail 100X (Thermo Scientific, #78430) added before lysis ...
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bioRxiv - Systems Biology 2021Quote: ... malate and tartrate) and the main fermentative by-products (ethanol, glycerol and 2.3 butanediol) were quantified using HPLC (Thermo Fisher Scientific, Waltham, MA) coupled with refraction index and UV/VIS (210 nm ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed once with PBS and before mounting on objective glass with 6% n-propyl gallate diluted in glycerol and 10x PBS and combined with ShandonTM Immuno-MountTM (Thermo Scientific, cat# 9990402) in a 1:12 ratio ...
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bioRxiv - Microbiology 2022Quote: ... the eluted protein was dialyzed against wash Buffer A + 5% glycerol overnight in 50K MWCO (Int and IntS12A) and 10K MWCO (Adi) Slide-A-Lyzer™ Dialysis Cassettes (Thermo Scientific™) with 20U/mg of 6xHis-SUMO protease ...
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bioRxiv - Molecular Biology 2019Quote: ... 10% glycerol and 5 mM β-mercaptoethanol) and then TEV cleaved for 2 hours at 16°C using AcTEV™ protease (Invitrogen, Germany). TEV elute was digested with RNace-It™ Ribonuclease Cocktail (Agilent Technologies ...
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bioRxiv - Microbiology 2019Quote: ... and resuspending in 500 µL lysis buffer (20 mM Tris-Cl, 150 mM NaCl, 15 mM MgCl2, 8% glycerol, 20 IU/mL SUPERase•In (ThermoFisher Scientific Cat# AM2696), 80 IU/mL Murine RNAse Inhibitor (New England BioLabs Cat# M0314S) ...
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bioRxiv - Systems Biology 2021Quote: ... malate and tartrate) and the main fermentative by-products (ethanol, glycerol and 2.3 butanediol) were quantified using HPLC (Thermo Fisher Scientific, Waltham, MA) coupled with refraction index and UV/VIS (210 nm ...
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bioRxiv - Cell Biology 2021Quote: ... 500 mM KCl, 5% glycerol, 50 mM maltose) and concentrated using an Amicon Ultra Centrifugal Filter Unit (50 kDa, 4ml) (Fisher Scientific, EMD Millipore). The protein was further purified by gel filtration chromatography (Superdex-200 ...
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bioRxiv - Biochemistry 2023Quote: ... Tissue was then coversliped with glycerol wet mount medium (Rs’ Science) for microscopic imaging using an EVOS™ M7000 Imaging System (brightfield, 20x objective; Invitrogen, Waltham, MA) was used for microscopy imaging.
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bioRxiv - Cell Biology 2023Quote: ... 5 % glycerol, 50 mM maltose) and were concentrated using an Amicon Ultra Centrifugal Filter Unit (50 kDa, 4 ml) (Fisher Scientific, EMD Millipore). Proteins were further purified by gel filtration chromatography (Superdex-200 ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was resuspended in Buffer A (20 mM HEPES, 20 mM KCl, ½ tablet Pierce Protease Inhibitor A32963 from Thermo Scientific, 10% glycerol) and desalted with either (i ...
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bioRxiv - Biophysics 2019Quote: ... The unbound Rep-X was washed off simultaneously and the unwinding reaction was initiated by adding the unwinding buffer (10 mM Tris-HCl, pH 8, 100 mM KCl, 2.5 mM MgCl2, 1 mM ATP [ThermoFisher Scientific], 10 % glycerol, 1 % BSA). For characterization of the maiden folded GQ conformation ...
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bioRxiv - Microbiology 2020Quote: ... all the strains were thawed and sub-cultured on Sabouraud Dextrose Agar (SDA) (Oxoid, Hampshire, UK) to obtain pure cultures and stored in 15% (vol/vol) glycerol (Fisher Scientific UK Ltd., England) at −80°C until further used ...
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bioRxiv - Biophysics 2019Quote: ... The unbound Rep-X was washed off simultaneously and the unwinding reaction was initiated by adding the unwinding buffer (50 mM Tris-HCl, pH 7.5, 100 mM KCl, 5 mM MgCl2, 1 mM ATP [ThermoFisher Scientific], 10 % glycerol, 1 % BSA). The unwinding reaction was quenched after a minute to capture the conformation of maiden folded RNA aptamers ...
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bioRxiv - Bioengineering 2020Quote: A single colony of the desired strain was inoculated in 25 mL pH 6 buffered Glycerol-complex (BMGY) medium (Pichia Expression Kit, Life Technologies, Carlsbad, CA, USA) and grown at 28 °C with 250 RPM shaking until saturation ...
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bioRxiv - Microbiology 2022Quote: ... ΔbioA strains were made luminescent by transformation with 100 ng pMV306G13+Lux+zeo and selection on 7H10 + 0.5% (v/v) glycerol + 10% (v/v) OADC agar plates containing 100 µg/mL zeocin (R25001, Thermo Fisher Scientific, Waltham, MA, USA). bioA rescue constructs were generated by transforming ΔbioA pMV306G13+Lux+zeo strains with pUV15-Tet-bioA and selecting on 7H10 + 0.5% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: Single colonies of the desired strain were used to inoculate 10 mL of Buffered Glycerol Complex Medium (BMGY) (Pichia Expression Kit, Life Technologies, Carlsbad, CA, USA) and grown at 28°C for two days with shaking at 250 rpm ...
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bioRxiv - Neuroscience 2023Quote: ... Embryos were mounted in a solution of 50% glycerol and 50% 1X PBS on glass microscope slides (Fisher Scientific, Selectfrost, 25×75×1.0 mm) with cover glass (Global Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... retinal tissues or retinal microvessels were mounted with a PBS/Glycerol mounting medium consisting of the nuclear staining Hoechst 33258 (Invitrogen™, 1:1000 dilution).
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bioRxiv - Microbiology 2023Quote: ... and their respective complemented strains cultured in 7H9 medium supplemented with 0.4% glycerol or sodium propionate were treated with CellROX Green (Life Technologies; final concentration, 5 μM) for 30 min at 37 °C ...
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bioRxiv - Neuroscience 2020Quote: ... 0.1% SDS and 10% glycerol) that was freshly supplemented with HALT protease and phosphatase inhibitor cocktail (Thermo Scientific, cat no: 78441, 1/10 total volume). Aliquots (2 µl ...
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bioRxiv - Genetics 2020Quote: 10 μl protein extract was mixed with 6 μl dilution buffer (1x PBS with 20% glycerol) and 2 μl streptavidin solution (Invitrogen, dissolved at 10 mg/ml in PBS), and incubated at room temperature for 10 minutes ...