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Citations for Thermo Fisher :
251 - 300 of 10000+ citations for BD 2 Mouse since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and then cultured with 30 U/mL rIL-2 (#34-8021-85, eBioscience) and activated with the mouse T-activator CD3/CD28 Dynabeads (Cat. 11452D, ThermoFisher) for 96 hours under the HG ...
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bioRxiv - Immunology 2020Quote: ... IFNα and IFNβ measurements were assessed in AMs supernatants using the IFNα/IFNβ 2-Plex Mouse ProcartaPlex™ immunoassay (ThermoFisher), according to the manufacturer’s instructions and read on a MagPix instrument (Luminex) ...
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bioRxiv - Bioengineering 2022Quote: ... Wells were rinsed with PBS and CD8 T cells were added at 1×106 cells/mL in complete RPMI supplemented with 50 U/mL IL-2 and mouse T-activator Dynabeads (ThermoFisher) at 1:1 ratio ...
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bioRxiv - Microbiology 2022Quote: ... 1 μl of Alexa Fluor 488 goat anti-mouse IgG (H+L) at 2 µg/ml (Life Technologies, cat#A11001) and 0.5 μl of 10 mg/ml Hoechst 33342 was added to the sample and incubated for 15 minutes at RT in the dark ...
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bioRxiv - Microbiology 2022Quote: ... USA) in combination with Alexa Fluor 594 (red)–labelled goat anti-mouse IgG (H+L; F(ab')2 fragment) (Invitrogen) targeting the RSE cell cytokeratins were used (52) ...
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bioRxiv - Molecular Biology 2023Quote: ... Fab were generated from monoclonal antibodies using the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Fisher). Antibodies were first digested into Fab in a Zeba Desalt Spin Column (Thermo Fisher ...
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ATM-Mediated Double-Strand Break Repair Is Required for Meiotic Genome Stability at High TemperaturebioRxiv - Plant Biology 2022Quote: ... and F(ab’)2-goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody Alexa Fluor Plus 555 (Invitrogen, A48287) were diluted to 10 μg/mL ...
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bioRxiv - Biophysics 2022Quote: ... Cells were then stained with secondary antibodies goat anti-mouse IgG conjugated AF647 (catalog no. A21235, Invitrogen, 2 mg/ml) with 1:800 dilution in 3% BSA for 2 h and washed with PBS 3 times for 5 minutes each ...
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bioRxiv - Neuroscience 2023Quote: ... incubation with primary antisera was followed by 2 hours of incubation with secondary antibody (1:350, goat anti-mouse IgG Alexa Fluor 488; Cat#A1101, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: The following secondary antibodies were used in this study: F(ab’)2-goat anti-mouse IgG Alexa 647 (Thermo Fisher Scientific Cat# A48289TR ...
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was extracted from mouse spinal cord segments (∼2 mm) rostral to the lesion epicenter 14 days after injury using TRIzol reagent (ThermoFisher) following the manufacturer’s instructions and methods previously described (21) ...
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bioRxiv - Neuroscience 2023Quote: ... with TBST and incubated for 2 hours with horseradish peroxidase coupled secondary goat anti-rabbit or anti-mouse IgG (1:5000, Invitrogen) in TBST ...
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bioRxiv - Cell Biology 2022Quote: ... and MIRO1/2 double-knockout mouse embryonic fibroblasts (MEFdko) (Lopez-Domenech et al., 2018) were grown in DMEM high glucose (ThermoFisher) supplemented with 10% fetal bovine serum (Gemini Bio-products ...
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bioRxiv - Cell Biology 2023Quote: ... C3H female mouse in origin) were cultured every 2-3 days using 0.25% Trypsin-EDTA and maintained in DMEM (GIBCO, 11965118) supplemented with 20% iFBS and 1% Penicillin/Streptomycin ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with anti-chicken Alexa Fluor 488 and/or anti-mouse Alexa Fluor 555 secondary antibodies (1:250) for 2 hours before mounting with Prolong Glass (Thermofisher). Confocal z-stacks were obtained by imaging on a Zeiss LSM 700 microscope in the UNC Microscopy Services Laboratory with an EC Plan Neofluar 40x objective using a 1.3 N.A ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were treated with 2 μl of anti-mouse CD16 (FcγII)/CD32 (FcγIII) Antibody (ThermoFisher, Cat. No. 14-0161-82) and incubated on ice for 30 minutes with mixing at 10 minute intervals ...
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bioRxiv - Bioengineering 2023Quote: ... TUBB4A and MUC5AC overnight at 4 °C and then incubated with Alexa 488-F(ab’)2 fragment of goat anti-mouse IgG (Invitrogen) for 2 h at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... a goat anti-mouse Alexa-488 conjugated antibody was applied (1:1000) for 2 h at room temperature (Life Technologies). Finally ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed 3X for 5 min in TBS followed by a 2-h incubation at room temperature with secondary antibodies [donkey anti-mouse Alexa Fluor 488: 10 μl/ml (ThermoFisher); donkey anti-rabbit Alexa Fluor 594 ...
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bioRxiv - Microbiology 2023Quote: ... SARS-CoV-2 Nucleocapsid antibody (produced in mouse) was procured from (MA5-35943/Invitrogen/Massachusetts, USA/1:1000-1:10,000) used as primary antibody ...
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bioRxiv - Biochemistry 2023Quote: ... in TSMBB or 5 µg/ml anti-mannose antibody (100-4G11-A) (50) followed by 2 µg/ml anti-mouse IgM AF 647 (Invitrogen); (iii ...
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bioRxiv - Cell Biology 2023Quote: ... Tissues from Ca1/2-Ca12/13 from each mouse were pooled and stored into RNAlater Reagent (Invitrogen, Carlsbad, CA, USA) at -80°C till isolation (n = 6 mice/genotype) ...
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bioRxiv - Immunology 2024Quote: ... After 2 hours incubation with HRP-conjugated secondary antibody (1:10,000; anti-mouse or anti-rabbit, Invitrogen #31430 and #31460) in TBST ...
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bioRxiv - Developmental Biology 2024Quote: ... goat-anti mouse Alexa Fluor 488 or 568) combined with 2 drops of NucBlue in 1 ml PBT (Invitrogen, RF37606), for 1-2 days ...
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bioRxiv - Physiology 2024Quote: Mouse DRG neuron cultures were loaded for 30 min at 37 °C with 3 µM Fura-2 AM (Invitrogen F1221) in ES containing also 0.02% Pluronic F-127 and left to recover for about 10 min in ES before recording ...
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bioRxiv - Immunology 2024Quote: ... IgGs from individual serum or pooled sera bound to the plates were detected after a 2-h incubation with goat anti– mouse-IgG–linked horseradish peroxidase (1:8,000 Invitrogen, USA). For measuring IgG isotypes ...
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bioRxiv - Immunology 2024Quote: ... naïve CD4+ T cells were enriched from homozygous TCR-transgenic OT-II (CD45.1/.2) donors using the Dynabeads Untouched™ Mouse CD4 Cells kit (Invitrogen). 1 x 105 OT-II cells were transferred intravenously (i.v. ...
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bioRxiv - Physiology 2021Quote: ... mouse anti-mouse GAPDH (Thermo Fisher, #AM4300), rabbit-anti-mouse LSD1 (Abcam ...
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bioRxiv - Immunology 2022Quote: ... 0.5% 2-mercaptoethanol (2-ME) (GIBCO) and 1% HEPES (GIBCO) ...
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bioRxiv - Cancer Biology 2021Quote: ... primary antibodies were detected using Alexa Fluor 680 F(ab’)2 fragments of goat anti-mouse immunoglobulin G (IgG) or anti-rabbit IgG (Life Technologies) diluted at 1:8,000 in Blocking buffer ...
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Developmental Biology 2021Quote: Single-stranded cDNAs were converted from 2 µg of total RNA extracted from mouse brains with the high-capacity cDNA reverse transcription kit (Applied Biosystems). Quantitative-PCR was conducted to determine transcript levels using gene-specific TaqMan probes (Applied Biosystems).
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bioRxiv - Cell Biology 2019Quote: ... 9101) were purchased from Cell Signalling. Mouse monoclonal anti tot-ERK1/2 (Cat. Nr. 13-6200) was purchased from Thermo Fisher. Mouse monoclonal anti-α-Tubulin (Cat ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed once and stained with goat anti-mouse IgG-Alexa Fluor 488 (2 μg/mL, # A11029, Thermo Fisher Scientific) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... The cells were washed and stained with 2 μg/mL goat anti-mouse IgG-Alexa Fluor 647 (# A21235, Thermo Fisher Scientific) in FACS buffer for 30 min ...
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bioRxiv - Immunology 2021Quote: ... at the concentration 1×106 cells per ml or Dynabeads Mouse T-Activator CD3/CD28 (ratio 1:2, Thermo Fisher Scientific) in the presence of protein transport inhibitor (BD GolgiStop™ ...
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bioRxiv - Molecular Biology 2021Quote: ... The supernatant was diluted with 400 μl of NET-2 buffer and antibody-conjugated Dynabeads M-280 Sheep anti-mouse IgG (ThermoFisher Scientific) beads were added ...
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bioRxiv - Molecular Biology 2022Quote: ... The membranes were incubated with horseradish Peroxidase-conjugated AffiniPure Goat Anti-Rabbit IgG and Goat Anti-Mouse IgG (ZSGB-BIO, Beijing, China) for 2 h and signals were observed using Super Signal West Pico (Thermo Scientific). Western blotting band intensity was quantified by densitometry analysis using ImageJ version 2x (NIH Image software ...
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bioRxiv - Molecular Biology 2019Quote: ... Cells were then washed 3x in PBS with 2% FBS and incubated with secondary antibody (Alexa Fluor 488-conjugated goat anti-mouse IgG) and fluorescently-tagged phalloidin (Molecular Probes), in a humidity chamber for 1hr ...
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bioRxiv - Neuroscience 2021Quote: ... and then incubated for 2 hours at room temperature in Alexa Fluor 488 donkey-anti-mouse (1:500 in TNB, Invitrogen, RRID:AB_141607). Following three washes with TNT ...
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bioRxiv - Molecular Biology 2021Quote: Fab preparation was performed using the same monoclonal antibodies used in ChIP experiments and the Pierce Mouse IgG1 Fab and F(ab’)2 Preparation Kit (Thermo Scientific), as described before27 ...
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bioRxiv - Molecular Biology 2019Quote: ... Membranes were washed with TBST and incubated for 1 h in TBST/2% BSA with secondary antibody (Immunopure goat anti-mouse # 31430, Thermo Scientific, and peroxidase-labeled affinity anti-rabbit # 04-15-06 ...
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bioRxiv - Pathology 2019Quote: ... Stimulation of T cells was achieved by adding 40 IU IL-2 and Dynabeads Mouse T-Activator CD3/CD28 (ThermoFisher Scientific) for 4 days ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were then washed and incubated at room temperature for 2 h in donkey anti-mouse alexa fluor 594 (1:1000, Life Technologies) and donkey anti-rabbit alexa fluor 488 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Triton X-100 with 5 % normal-goat-serum and unconjugated F(ab′)2 goat anti-mouse IgG (H+L) antibody (1:500, Thermo Scientific), at 4 °C for 2 h ...
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bioRxiv - Immunology 2021Quote: ... Cells were then washed twice in PBS then suspended in goat anti-mouse IgG (H&L) cross-adsorbed secondary antibody Alexa Fluor 488 (2 mg mL−1; Thermofisher Scientific) in PBS 0.1 % BSA for 45 min at 4 °C ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were incubated for 30 min with the secondary antibody (2 µg/mL; Goat α mouse-Alexa 647, ThermoFisher, A-21235). dSTORM imaging was performed on a LEICA DMi8 mounted on an anti-vibrational table (TMC) ...
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bioRxiv - Biochemistry 2021Quote: ... GP-1 was detected by incubating the slide with 2 μg/mL of Alexa Fluor 633 goat-anti-mouse (H+L) antibody (Invitrogen A21050) for 3 h ...
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bioRxiv - Neuroscience 2021Quote: ... immunogen SARS-CoV-2 spike protein that interact with HeLa cell expressed SARS-CoV-2) and anti ACE2 antibody (ACE2 Recombinant Rabbit Monoclonal Antibody (Cat# MA532307; interact with mouse ACE2) were obtained from Life Technologies Corporation.
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bioRxiv - Neuroscience 2021Quote: ... the sections were incubated with goat anti-mouse IgG1 Alexa 488 secondary antibody (1:500 for 2 h at 4°C; Invitrogen by ThermoFisher Scientific) in solution with PBS ...