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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for Autophagy Related 4B Cysteine Peptidase ATG4B Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: ... We blocked cysteine residues using 2 mM Tris (2-carboxyethyl) phosphine hydrochloride (TCEP) (Thermo Fisher Scientific) at 37 °C for 30 min followed by alkylation with 10 mM 2-iodoacetamide (IAA ...
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bioRxiv - Biochemistry 2024Quote: ... Conditioned media for methionine and cysteine were prepared as follows: 10% dialyzed FBS (Gibco, Carlsbad, USA), 100 U/ml penicillin ...
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bioRxiv - Molecular Biology 2022Quote: ... were plated into each well of a 24-well plate and transfected with 50 ng pCAG-hyPBase (Ochiai et al., 2015) and 450 ng Intein-related plasmids (Tables S1 and S2) using Lipofectamine 3000 (L3000015, Life Technologies, Carlsbad, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Equal volumes of cDNA were used for quantifying various cytokine immune response-related factor cDNAs via qRT-PCR using the StepOne real-time PCR system (Applied Biosystems, Foster City, California). qRT-PCR analysis was performed using KOD SYBR qPCR Mix (Toyobo Co ...
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bioRxiv - Epidemiology 2019Quote: Genotyping of UK Biobank samples was conducted with two closely related arrays (Affymetrix using a bespoke BiLEVE Axiom array and Affymetrix UK Biobank Axiom array) and is described in detail elsewhere [29,30] ...
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bioRxiv - Developmental Biology 2020Quote: ... as directed by the manufacturer and employing oligo d(T)16 (for Fig. 1d & 4b) (Invitrogen™; N8080128) or random hexamers (for Fig ...
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bioRxiv - Immunology 2021Quote: ... Dinutuximab and dinutuximab LALA-PG were then purified using recombinant protein A-Sepharose 4B (ThermoFisher Scientific, Waltham, MA), buffer exchanged into antibody buffer (20mM L-Histidine ...
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bioRxiv - Cell Biology 2020Quote: Purified AHs were covalently labeled via cysteines using Alexa C5 maleimide probes (either 488 or 568; Thermofisher). Plin4 12mer ...
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bioRxiv - Microbiology 2019Quote: The single cysteine residue in RepA-WH1(A31V,K132C) was labelled with Alexa Fluor 488 maleimide (Invitrogen) using thiol chemistry ...
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bioRxiv - Plant Biology 2022Quote: ... The cysteine residues were reduced in 100 mM DTT and alkylated in 200 mM iodoacetamide (Acros Organics). 20 ug of beads (1:1 mixture of hydrophilic and hydrophobic SeraMag carboxylate-modified beads ...
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bioRxiv - Systems Biology 2023Quote: ... with 100% MetR medium (complete DMEM without L-methionine and L-cysteine, ThermoFisher, 11960-044, 10% FCS). For example ...
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bioRxiv - Cell Biology 2024Quote: ... CPT labelled cysteine peptides were purified using the High-select Fe-NTA phosphopeptide enrichment kit (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2019Quote: ... C9-3B and C9-4B were generated using the CytoTune™-iPS 2.0 Sendai Reprogramming Kit (ThermoFisher Scientific, A16517). qRT-PCR for Sendai virus clearance was performed and did not detect residual Sendai virus RNA in any of the tested iPSC lines ...
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bioRxiv - Microbiology 2021Quote: ... external cysteine residues were blocked with 107 μM 4-acetamino-4’-maleimidylstilbene-2,2’-disulfonic acid (AMS, Thermo Scientific) for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: HeLaM-iORF7a cells were incubated in cysteine- and methionine-free Dulbecco’s modified Eagle’s medium supplemented with 5% dialyzed fetal bovine serum (Invitrogen), 1% Glutamax ...
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bioRxiv - Cell Biology 2019Quote: ... Samples were then cooled to room temperature and free cysteines were alkylated with 15 mM iodoacetamide (ThermoFisher Scientific) in the dark at room temperature for 30 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... protein extracts were incubated with the specific antibody overnight at 4 °C after adjusting the buffer to a final concentration of 150 mM NaCl and 0.5% NP-40 and then incubated for 1 h with Protein A-Sepharose™ 4B (Invitrogen), washed extensively ...
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bioRxiv - Cell Biology 2020Quote: ... For cycloheximide treatment began 6h before cells were washed twice with cysteine/methionine-free DMEM (Thermo Fisher Scientific, 21013024), incubated in 2ml of cysteine/methionine-free DMEM ...
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bioRxiv - Cell Biology 2021Quote: ... Alexa Fluor 647 C2 maleimide was conjugated to the purified protein via the novel cysteine residue according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Cell Biology 2021Quote: ... culture medium was removed and replaced by pre-mixed medium containing 0.95 × volume 100% AHA medium (DMEM, high glucose, no glutamine, no methionine, no cysteine (Gibco) was supplemented with 10% fetal bovine serum ...
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bioRxiv - Cell Biology 2022Quote: ... Four sets of three retinas per genotype were incubated in DMEM without methionine or cysteine (ThermoFisher Scientific. cat# 21013) but supplemented with 200 mM cysteine (SigmaAldrich C7352 ...
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bioRxiv - Biochemistry 2020Quote: The Dip1 construct used for site specific labeling with the cysteine-reactive Alexa Fluor 568 C5 maleimide (Thermo Fisher) had the six endogenous cysteine residues in Dip1 mutated to alanine and a single cysteine added to the short N-terminal polypeptide sequence on the end of the coding sequence as previously described (Balzer et al. ...
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bioRxiv - Neuroscience 2019Quote: ... The antigen peptide with N-terminus Cysteine (NH2-CGGGGAAPGPPRHG-COOH) was coupled with keyhole limpet hemocyanin (Thermo fisher 77600). The antigen was injected into rabbit and antisera were collected after primary and several booster injections ...
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bioRxiv - Plant Biology 2022Quote: Protein samples were reduced with Tris(2-carboxyethyl)phosphine (TCEP) and cysteines alkylated with Iodoacetamide (IAA, Thermo Fisher, Germany). Subsequent proteolytic digests were performed using S-TRAPs (Protifi ...
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bioRxiv - Molecular Biology 2022Quote: The recombinant abGFP4 nanobody against GFP with a C-terminal cysteine was covalently coupled to SulfoLink resin (Thermo Scientific) consisting of cross-linked agarose beads with iodoacetyl groups ...
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bioRxiv - Synthetic Biology 2023Quote: Tetra-cysteine (TC) tagged proteins (CCPGCC) were site-specifically labelled using the TC-ReAsH II TC detection dye (Invitrogen). TC-HERD-2.2–TnaA and TC-HERD-2.2–FMO (100 μM ...
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bioRxiv - Biophysics 2024Quote: ... and incubating the GHSR containing a single reactive cysteine at position 711.60 29 with Alexa Fluor 488 maleimide (ThermoFisher) at a 1:1.5 receptor-to-dye molar ratio ...
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bioRxiv - Cell Biology 2020Quote: GST pull-downs were performed by incubating purified GST tagged proteins (5 µg) with 20 μl dry volume of glutathione agarose 4B beads (Thermo Scientific) for 1 h under rotation at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... were purified from supematant of HB95 hybridoma cells (ATCC® HB-95) using protein-A sepharose 4B (Pro-A) beads (Invitrogen) and cross-linked to pro-A beads ...
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bioRxiv - Molecular Biology 2023Quote: About 3 ml of bleed 3 was diluted in 7 ml of sterile 1X PBS and passed through 0.2 μM filter and incubated with recombinant protein G sepharose 4B beads (Life Technologies 101242) at 4℃ overnight ...
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Oxidative and non-oxidative active turnover of genomic methylcytosine in distinct pluripotent statesbioRxiv - Cell Biology 2020Quote: ... For metabolic labelling experiments with m+4Met under all conditions mPSC basal medium was generated with 0.2 mM L-Methionine- and L-Cysteine-free DMEM (Sigma or Life Technologies) supplemented with m+4Met ([methyl-13C,d3] ...
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bioRxiv - Molecular Biology 2019Quote: ... were used for studies of INS-1 cells treated with CSE or AC extract with or without 5 mM N-Acetyl Cysteine (NAC) from Invitrogen; Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... in Dulbecco’s modifiedEagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAXTM (ThermoFisher Scientific). Cells were subsequently lysed in radioimmunoprecipitation assay (RIPA ...
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bioRxiv - Neuroscience 2020Quote: ... After washing chase medium (DMEM containing 10% FCS, Glutamax, P/S, 1 mg/mL methionine and 1 mg/mL cysteine (Gibco)) was added to cells and incubated for different time points ...
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bioRxiv - Neuroscience 2020Quote: ... after 24 h of transfection cells were washed twice with pre-warmed pulse labeling medium (methionine and cysteine free DMEM (Gibco) containing 10% dialyzed FBS (Thermo Scientific) ...
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bioRxiv - Synthetic Biology 2021Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were incubated for one hour in methionine- and cysteine-free DMEM (#D0422, MilliporeSigma) with 10% dialysed serum (#26400044, Gibco). The medium was then replaced with methionine- and cysteine-free DMEM containing [35S]-protein labeling mix (20 μCi/mL ...
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bioRxiv - Neuroscience 2022Quote: ... For optimal incorporation of the amino acid azidohomoalanine (AHA), cells were methionine-deprived for 2.5 h in methionine-free medium (DMEM without methionine, glutamine, and cysteine (Gibco, 21013024), supplemented with 0.5% FBS ...
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bioRxiv - Microbiology 2020Quote: ... These two proteins were labeled at the cysteine using thiol-reactive BODIPY™ FL N-(2-Aminoethyl) Maleimide (Thermo Fisher). The proteins were mixed with 10-fold excess BODIPY™ FL N-(2-Aminoethyl ...
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bioRxiv - Immunology 2021Quote: ... 2-43 and H4 by digestion with Papain in the presence of the reducing agent 30 mM cysteine and were purified by affinity chromatography on protein A following the manufacturer’s protocols (Thermo fisher). The purity of the resultant Fabs was analyzed by SDS-PAGE ...
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bioRxiv - Biophysics 2023Quote: ... The reaction was stopped with 5 mM L-cysteine and unreacted fluorophore again removed on a Zeba spin column (ThermoFisher). The degree of labeling was determined using the known extinction coefficients of the protein and both fluorophores at their absorbance maxima ...
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bioRxiv - Biophysics 2024Quote: ... The reaction was then stopped with 5 mM L-cysteine and unreacted fluorophore removed on a Zeba Spin column (ThermoFisher). Labeling with the Tb-cryptate for the LRET experiments was carried out by incubating the β-arrestin 1 with 2 equivalents of Lumi-4 Tb maleimide (CisBio ...
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bioRxiv - Biochemistry 2024Quote: ... Labeling of WT and p.R196H actin at cysteine–374 using N–(1–pyrene)iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) followed established protocols (55).
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bioRxiv - Biophysics 2023Quote: ... The Tm of purified CFTRs (0.7 µM) was monitored in the presence of 1 µM Bodipy- FL-cysteine (Life Technologies) at 505 nm excitation and 513 nm emission to determine the accessibility of 19 Cys of CFTR88 ...
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bioRxiv - Cell Biology 2024Quote: ... and cross-linked by incubation for 5-min on ice with ice-cold PBS containing 40 μM of cysteine-specific cross-linker BMB (ThermoFisher). The reaction was stopped by washing the cells with PBS with dithiothreitol (1 mM) ...
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bioRxiv - Biochemistry 2019Quote: CMG2-GST (AlexaFluor 488 or biotin) and PA-E733C (AlexaFluor 546) were conjugated on a single reactive cysteine with maleimide-labels (Thermo Fisher) after GST was cleaved by using thrombin ...
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bioRxiv - Biophysics 2022Quote: Biotinylated hLRP6E1E2 was tetramerized with Alexa 647 labeled streptavidin (SA647, streptavidin with a cysteine residue produced in house, and labeled with Alexa Fluor 647 C2 Maleimide, Thermo Fisher) and cells were stained with 30-200 nM hLRP6E1E2-SA647 tetramer (120-800 nM hLRP6E1E2 as monomer) ...
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bioRxiv - Biophysics 2020Quote: The tau cysteine mutants were buffer exchanged into 20 mM NaPi buffer (pH 8) via 7k MWCO Zeba spin desalting columns (Thermo Fisher). 200 μL of 50 μM protein aliquots were reacted with 50 molar equivalents of methyl 2,5-dibromopentanoate (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... in Dulbecco’s modified Eagle’s medium lacking methionine and cysteine and supplemented with 10% of dialyzed fetal bovine serum and 1X GlutaMAX™ (ThermoFisher Scientific). Simultaneously ...
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bioRxiv - Plant Biology 2022Quote: ... The presence of active serine hydrolases and cysteine proteases was measured by ABPP using the FP-TAMRA probe (Thermo Fisher Scientific) and TK009 (see above) ...