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Citations for Thermo Fisher :
51 - 100 of 10000+ citations for Anti CD44 SAP Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2021Quote: ... The PCR products were purified with 3 μl Exo/SAP and then cycle-sequenced with the BigDye™ Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific) according to manufacturer instructions with BP15851 (M13F ...
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bioRxiv - Genomics 2021Quote: ... The PCR products were treated with Exo-Sap (ExoSAP-IT™, Thermo Fisher, USA) to remove unutilized primers and dNTPs ...
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bioRxiv - Microbiology 2021Quote: Purified substrates and cleavage product mixtures were dephosphorylated by incubating with SAP (ThermoFisher, USA) at 37°C for 1 h according to the manufacturer’s protocol ...
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bioRxiv - Zoology 2021Quote: ... we purified the amplified DNA using USB Exo-SAP-IT (Affymetrix, Santa Clara, CA) and bidirectionally sequenced the amplicons using an ABI 3130 DNA Analyzer (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... the resulting DNA from the PCR reaction was purified via EXO-SAP cleanup (Affymetrix). Individual EXO-SAP reactions consisted of 1.55ul dH2O ...
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bioRxiv - Neuroscience 2022Quote: ... CD44-PERCP CY5.5 (Invitrogen, Waltham, MA; 1:200), CD49D-PE dazzle 594 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... CD44 (catalog 48-0441-80; Thermo Fisher Scientific); IFNγ (catalog 25-7311-82 ...
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bioRxiv - Microbiology 2021Quote: ... followed by purification using the Exo-SAP-IT PCR product clean-up reagent (Thermofisher, Australia) according to manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... sections were incubated for 30 minutes with 0.5 µg/ml rat anti-CD44 clone IM7 (Thermo Scientific). For the survivin IHC a survivin rabbit mAB (Cell Signaling ...
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bioRxiv - Developmental Biology 2021Quote: ... CD44 (1:200; Thermo Fisher Scientific, 17-0441-82), CD36 (1:200 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD44 PerCPC-Cy5.5 (1:400, IM7, Thermo Fisher Scientific) and CD25 Alexa Fluor 488 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... or CD44 (Cat# MA513890, ThermoFisher Scientific, Waltham, MA, USA) primary antibody overnight ...
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bioRxiv - Immunology 2020Quote: ... CD44-APC-Cy7 and LIVE/DEAD Aqua (Thermo Fisher), cells were fixed with 10% neutral buffered formalin (containing 4% paraformaldehyde ...
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bioRxiv - Immunology 2020Quote: ... CD44- Thy1.2+ thymocytes were sorted into Trizol (Life Technologies) using a Beckman Coulter MoFlo XDP cell sorter ...
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bioRxiv - Cancer Biology 2023Quote: ... 1:100) or CD44-Alexa Fluor 700 ((Thermo Fisher Scientific Cat# 56-0441-82 ...
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bioRxiv - Immunology 2023Quote: ... CD4 (RM4-5), (anti-CD90.2 [30-H12], anti-CD44 [IM7], and anti-CD27 [LG-7F9]) (all from eBioscience/Invitrogen and Biolegend, San Diego, CA). Phenotype of polyclonal CD4+ T cell subsets and Plasmodium B5-specific CD4+ T cell subsets were identified with gating strategy as schematically depicted in S7 Fig ...
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bioRxiv - Developmental Biology 2022Quote: ... and CD44 (1:100, Thermo Fisher Scientific, 14-0441-82), in blocking solution were used at 4°C overnight ...
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bioRxiv - Developmental Biology 2020Quote: ... Immunofluorescence staining was performed with a rat-CD44 antibody (Invitrogen) as described previously (68) ...
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bioRxiv - Immunology 2021Quote: ... cells were stained for extracellular markers (CD3e, CD8a, B220 and CD44) and viability dye ( Near IR dead cell staining kit, Invitrogen. Cells were then stained for intracellular IFNγ ...
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bioRxiv - Immunology 2023Quote: The sorted splenic CD4+CD44+ cells in TRIzol reagent (Invitrogen, #15596018) were pipetted up and down 2-3 times to homogenize the cell lysate ...
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bioRxiv - Evolutionary Biology 2023Quote: ... after enzymatic clean up with Exo-SAP-IT PCR Product Cleanup Reagent (Thermo Fisher Scientific, Waltham, MA, USA) applying a 10x dilution of the Exo-SAP-IT but otherwise following the manufactureŕs instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Full-length Venus-Flag-tagged CD44 and GST-tagged MSN for mammalian expression were generated using Gateway© cloning kit (Invitrogen). The vector backbones are pDEST27 vector (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen; Carlsbad, CA, USA) for 1 hr at 37°C ...
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bioRxiv - Immunology 2022Quote: ... CD44 (1M7) and magnetic Dynabeads™ M-280 Streptavidin (Thermofisher cat#:11206D).
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bioRxiv - Biochemistry 2024Quote: ... CD44 was immobilized on High Capacity Neutravidin Agarose (Thermo Fisher Scientific, 29204) by incubating reactions on agarose for 1 hour at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... The blots were incubated separately either with mouse monoclonal anti-human CD44 (156-3C11) antibody (Cat# MA513890, ThermoFisher Scientific, Waltham, MA, USA), mouse monoclonal anti-human CD63 antibody (Cat# ab8219 ...
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bioRxiv - Immunology 2019Quote: ... In some groups culture media was supplemented with recombinant osteopontin (2 μg/ml) (R&D) or anti-CD44 (5 μg/ml) (Thermofisher; clone IM7). Cells were cultured in 5% CO2 at 37°C ...
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bioRxiv - Immunology 2019Quote: ... total PBMC or IEL were cultured in the presence or absence of recombinant human osteopontin (2 μg/ml) (R&D) and anti-human-CD44 (5 μg/ml) (Thermofisher; clone IM7).
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bioRxiv - Genomics 2023Quote: The double-stranded linkers were converted into single-stranded to successively allow the second strand synthesis by Shrimp Alkaline Phosphatase (1 U/μl SAP, catalog num. 78390, Affymetrix) and Uracil-Specific Excision Reagent (1 U/μl USER ...
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bioRxiv - Immunology 2023Quote: ... The total isolated protein from splenic CD4+CD44+ cells was quantified using Bicinchoninic acid (BCA) Protein Assay Kit (Pierce, Thermo Scientific, USA, #23225).
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bioRxiv - Immunology 2022Quote: ... They were then washed with CM10 and extracellular staining was performed using anti-CD44-PerCP Cy5.5 (Invitrogen 45-0441-82: Clone: IM7, eBioscience), anti-TCRβ-APC ef780 (Invitrogen 47-5961-82 ...
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bioRxiv - Neuroscience 2020Quote: ... The resulting cDNA underwent another final second round IVT step at 37 °C overnight and followed EXO-SAP treatment (Affymetrix 78200) for 15 minutes at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... CD44 and CD62L and then incubated with 5 μM Fluo-4 AM (Molecular Probes) for 15 min at 37°C ...
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bioRxiv - Biophysics 2024Quote: ... CD44 (Hs00153304_m1) and the housekeeping genes GAPDH (Hs99999905_m1) and GusB (Hs99999908_m1) were from Thermofisher. Real time PCR were carried out on the 7500 Real-Time PCR System (Thermo Fisher) ...
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bioRxiv - Genomics 2020Quote: ... the aRNA was treated with 6 μL EXO-SAP (ExoSAP-IT™ PCR Product Cleanup Reagent, Thermo Fisher Scientific, Cat. # 78200.200.UL) at 37°C for 15 min followed by fragmentation with 5.5 μL fragmentation buffer (200 mM Tris-acetate (pH 8.1) ...
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bioRxiv - Cell Biology 2020Quote: ... The following antibodies were used: CD44-FITC (0.25 μg/106 cells, 110441-81, Thermo Fisher), CD140a-PE (10 μl/106 cells ...
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bioRxiv - Bioengineering 2022Quote: ... trypsinized HUVECs were treated with medium containing CD44 antibody (2 μg/mL, MA5-13890, Invitrogen) for 30 minutes at room temperature prior to seeding ...
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bioRxiv - Cell Biology 2019Quote: ... The CD44s plasmid or an empty pcDNA3.1 mammalian expression vector (Invitrogen/ThermoFisher Scientific, Carlsbad, CA) was amplified using One Shot™ Top10 chemically competent E ...
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bioRxiv - Cell Biology 2019Quote: ... The CD44s plasmid or an empty pcDNA3.1 mammalian expression vector (Invitrogen/ThermoFisher Scientific, Carlsbad, CA) was amplified using One Shot™ Top10 chemically competent E ...
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bioRxiv - Plant Biology 2021Quote: 5 μg of RNA from 5-day-old seedlings was first treated with 1 unit of Shrimp Alkaline Phosphatase (SAP; USB Products, Affymetrix, Inc.; Cleveland, OH, USA) in 1X SAP buffer provided and supplemented with 1 mM DTT and 60 units RNaseOUT (Invitrogen ...
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bioRxiv - Plant Biology 2021Quote: ... One millilitre of fresh sap or control sample was freeze-dried using a SpeedVac concentrator unit (model Savant SPD121P, Thermo Scientific, Waltham, MA, USA) at 40 □ ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were incubated overnight at 4°C with antibodies directed against CD44 (Thermo Scientific, MA4400; 1:200) and CD56 (Leica Biosystems ...
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bioRxiv - Evolutionary Biology 2020Quote: Cells in 6-well plates were transfected with 1 ug human or cow CD44-promoter-pNL2.1 or empty vector pNL2.1 using Lipofectamine 3000 (Invitrogen) as manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2019Quote: shRNA sequences for ITGAV (integrin αv) and CD44 were obtained as glycerol stock in pTRIPZ inducible plasmids (Thermo Scientific). For transduction ...
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bioRxiv - Immunology 2023Quote: ... mouse lymph node cells were incubated with biotin-conjugated antibodies to CD44 (IM7) and CD25 (PC61.5) (both from ThermoFisher), CD11c (N418) ...
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bioRxiv - Cell Biology 2020Quote: Small interfering RNAs (siRNAs) specifically targeting human CD44 (siRNA IDs s2681 and s2682) were obtained from Life Technologies (Carlsbad, CA, USA). DPSCs (1×105 per well ...
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bioRxiv - Genetics 2021Quote: ... were used to amplify a 323 bp region of exon 1 of the CD44 gene spanning the cut site for 4 cycles using Phusion Hot Start II DNA Polymerase (Thermo Fisher Scientific). PCR products were purified with Ampure XP beads (Beckman Coulter ...
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bioRxiv - Neuroscience 2020Quote: ... Anti-polyvalent HRP-Streptavidin Kit (Thermo Scientific) with DAB was used for the visualization of antigenic sites.
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bioRxiv - Neuroscience 2020Quote: ... clone 53-6.7), CD44 APC (1:300 or 1:400, clone IM7), FoxP3 (1:40, clone FJK-16s) (eBioscience Thermo Fisher Scientific, Waltham, MA, USA); CD69 APC (1:20 ...
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bioRxiv - Developmental Biology 2024Quote: ... Flow cytometry was used to evaluate the efficiency of differentiation to definitive endoderm at day 3 using the endoderm markers CXCR4 and c-KIT (Anti-human CXCR4 PE conjugate, Thermo Fisher, MHCXCR404,1:20; Anti-human c-kit APC conjugate, Thermo Fisher, CD11705 ...