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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for Anti CAR Antibody since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and CD19.CAR-T cells were labeled with biotinylated anti-Fab antibodies (ThermoFisher Scientific, 31803). The co-culture was then mixed with CELLection Biotin Binder (ThermoFisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... For samples that were mixed with protein (BSA, human CAR, mouse CAR [Fisher Scientific]) prior to attachment ...
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bioRxiv - Molecular Biology 2024Quote: ... each membrane was probed for using the following primary antibodies: mouse anti-CFTR 596 (1:1000) (UNC) or CAR anti-rabbit (CXADR) (1:100) (PA5-31175, Invitrogen, Waltham, MA) was applied for 2 hours ...
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bioRxiv - Immunology 2021Quote: ... Co-expression of GFP and the CAR construct was verified by flow cytometry staining of the CAR using an anti-mouse IgG (H+L) antibody (A21235, Invitrogen).
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bioRxiv - Cell Biology 2023Quote: ... and CAR (Cat.No. MA5-29208, Invitrogen). The antibodies were diluted in 0.1% BSA in D-PBS +/+ (1:100 for primary antibodies ...
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bioRxiv - Immunology 2024Quote: ... 2-mercaptoethanol (GIBCO, car 21985-023), supplemented with 1,000 U/mL mouse IL-2 (PeproTech ...
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bioRxiv - Bioengineering 2023Quote: ... CAR detection reagents were fluorescently labeled with the DyLight 650 Microscale Antibody Labeling Kit (Thermo Fisher). Anti-FLAG (BioLegend ...
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bioRxiv - Biophysics 2019Quote: ... K562 feeder cells expressing the cognate CAR antigen ROR1 (K562-ROR1) were used to stimulate CAR T cells and maintained in 1640 RPMI media (Life technologies) supplemented with 10 % FCS (Merck) ...
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bioRxiv - Bioengineering 2020Quote: FACS-purified CD3-CAR+ or CD3-CAR- CD4+ T cells (8 x 106 each) were lysed in Pierce™ IP Lysis Buffer (ThermoFisher) supplemented with Complete Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Cancer Biology 2022Quote: ... HEK293T packaging cells were transfected with the CAR expressing plasmids (GV401-CD19-CAR) together with lentiviral packaging plasmid using Lipofectamine Reagent (Thermo Fisher Scientific). After 48 h ...
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bioRxiv - Immunology 2019Quote: ... and the pLV vectors encoding ΔLNGFR-P2A-CD19-BBz CAR or BBz-CAR-mCherry using 10 μL of Lipofectamine2000 (Thermo Fisher Scientific, USA). Two days after transfection ...
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bioRxiv - Immunology 2023Quote: ... and CAR plasmids were transfected into HEK293T host cells (in Gibco DMEM HG medium containing 10% FBS ...
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bioRxiv - Bioengineering 2022Quote: ... CAR T-cells were labelled with cell trace violet (Invitrogen, Waltham, MA) prior to using them for perfusion experiments ...
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bioRxiv - Immunology 2023Quote: ... CAR-T cells were lysed in M-PER buffer (Thermo Scientific; 78501) with protease inhibitors (MilliporeSigma ...
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bioRxiv - Synthetic Biology 2024Quote: ... CAR-expressing T cells were stained 1:1000 with CellTrace CFSE (ThermoFisher #C34570) for 20 minutes at 37C and then washed with DPBS ...
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bioRxiv - Immunology 2023Quote: ... Phoenix-AMPHO (ATCC) were transfected with SFG-CAR constructs using lipofectamine 3000 (ThermoFisher), virus contained medium (VCM ...
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bioRxiv - Bioengineering 2021Quote: ... CAR-T cell quantification was performed using the CountBright Absolute Counting Beads (Thermo Fisher). Flow cytometry was performed on a BD Fortessa and analyzed on FlowJo version 10.7.1.
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bioRxiv - Immunology 2023Quote: CAR-T cells were labeled with 0.2 μM carboxyfluorescein succinimidyl ester (CFSE, Invitrogen, ThermoFisher), washed ...
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bioRxiv - Immunology 2023Quote: CAR-T cells were labeled with 0.2 μM carboxyfluorescein succinimidyl ester (CFSE, Invitrogen, ThermoFisher), washed ...
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bioRxiv - Synthetic Biology 2023Quote: ... The number of CAR T cells were quantified using CountBright™ Counting Beads (ThermoFisher; C36995). For the restimulation experiments ...
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bioRxiv - Cancer Biology 2023Quote: ... mRNA CAR T cells were stained with CellTrace™ CFSE Cell Proliferation Kit (Thermo Fisher Scientific) and co-cultured with target cells (E:T 1:1 ...
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bioRxiv - Cancer Biology 2021Quote: mRNA encoding CAR constructs were synthesized from the pTEV vectors using Megascript T7 transciption kit (AM1334, Invitrogen) and replacing UTP with 1-methylpseudouridine triphosphate (N-1081 ...
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bioRxiv - Cancer Biology 2022Quote: CAR and NT-T cells were labeled with CFSE (#C34554, CellTrace CFSE Cell Proliferation Kit Molecular Probes) according to the manufacturer’s instructions and at 1μM working concentration ...
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bioRxiv - Immunology 2023Quote: Purified CAR-T cells were stained in PBS with 10µM Cell Proliferation Dye eFluor™ 450 (ThermoFisher) at 37°C for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... The CAR constructs were transfected to Plat-A packaging cell line via Lipofectamine 3000 reagent (Thermo Fisher Scientific) and retroviral supernatant was collected at 48 and 72 hours post-transfection ...
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bioRxiv - Immunology 2019Quote: ... For the assessment of CAR T cell proliferation by CellTrace™ Violet (CTV; Molecular Probes, Thermo Fisher Scientific) dilution ...
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bioRxiv - Immunology 2019Quote: ... For the assessment of CAR T cell proliferation by CellTrace™ Violet (CTV; Molecular Probes, Thermo Fisher Scientific) dilution ...
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bioRxiv - Immunology 2023Quote: ... was added into the plasmid encoding HA-28z CAR (10 ug) suspended in Opti-MEM (500 uL, Gibco), incubated for 30 min at room temperature in the dark ...
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bioRxiv - Immunology 2024Quote: Total RNA from CAR and TCR/CD28 activated Tregs 24h post-activation was isolated using Trizol (ThermoFisher Scientific), according to manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Plasmids containing the full CAR constructs were used to transform One Shot Top10 chemically competent cells (Invitrogen, C404003), and bacteria were grown overnight (ON ...
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bioRxiv - Biochemistry 2022Quote: ... and secondary antibody (Invitrogen anti-mouse antibody) binding ...
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bioRxiv - Immunology 2020Quote: Non-transduced and CAR T cells were stained at 37°C with either 1μM 5-chloromethylfluorescein diacetate (CMFDA; Invitrogen) for 5 minutes or 125nM calcein red orange for 10 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... Sorted and rested CAR T cells were stained separately with eBioscience Cell Proliferation Dye eFluor 450 (1:4000, ThermoFisher) or Calcein AM (1:3000 ...
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bioRxiv - Immunology 2024Quote: ... CAR T cells were stained with either Calcein Green or Cell Proliferation Dye eFluor670 (Thermo Fisher 65-0840-85) (same procedure used for motility assay ...
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bioRxiv - Immunology 2021Quote: ... secondary antibodies (AF488 donkey anti-rat, AF647 goat-anti rabbit antibody & AF557 donkey anti-mouse antibody, all Thermo Fisher Scientific) were applied in the dark for one hour at room temperature ...
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bioRxiv - Immunology 2021Quote: ... anti-p24 monoclonal antibody (AG3.0) and anti-GAPDH monoclonal antibody (Life Technologies) followed by goat anti-mouse HRP-conjugated secondary antibody (Sigma).
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bioRxiv - Immunology 2021Quote: ... anti-p24 monoclonal antibody (AG3.0) and anti-GAPDH monoclonal antibody (Life Technologies) followed by goat anti-mouse HRP-conjugated secondary antibody (Sigma ...
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bioRxiv - Cell Biology 2019Quote: ... Anti-V5 antibody (Invitrogen) was added and the suspension incubated at 4 °C overnight ...
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bioRxiv - Cell Biology 2020Quote: ... Anti-GFP antibodies (Invitrogen) or Anti-HA antibodies (Abcam ...
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bioRxiv - Cell Biology 2019Quote: ... anti-Pgk1 antibody (Invitrogen), anti-porin (Invitrogen) ...
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bioRxiv - Molecular Biology 2021Quote: ... Anti-V5 antibody (Invitrogen) was diluted 1:5,000 ...
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bioRxiv - Biophysics 2022Quote: ... anti-IgG antibodies (Invitrogen). Actin was labeled with phalloidin-TRITC (Sigma ...
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bioRxiv - Cell Biology 2022Quote: ... Anti-GAPDH antibody (Invitrogen) 1:1000 dilution in 5% BSA – TBST ...
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bioRxiv - Microbiology 2022Quote: ... Anti-His6 antibody (Invitrogen) was used at a final concentration of 1:1000 ...
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bioRxiv - Biophysics 2022Quote: ... anti-IgG antibodies (Invitrogen). Samples were permeabilized with 0.5 % Triton X-100 (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Anti-species antibodies (ThermoFisher) labelled with Alexa Fluor 568 (red ...
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bioRxiv - Biophysics 2020Quote: ... Anti-Flag and anti-γH2AX antibodies (Invitrogen) in 3% BSA in 4x SSC buffer were incubated 1 hr ...
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bioRxiv - Biophysics 2021Quote: ... The antibodies used include anti-flag monoclonal antibody (mouse, Abcom) and anti-pgk1 Polyclonal Antibody (rabbit, Invitrogen). The secondary antibodies used include Peroxidase Affini Pure goatanti-mouse IgG and goat anti-rabbit IgG (Jackson) ...
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bioRxiv - Cell Biology 2022Quote: ... and secondary antibodies goat anti mouse 488 and anti-rabbit 568 antibodies (Invitrogen).
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bioRxiv - Molecular Biology 2024Quote: ... and: anti-mouse antibody (A11032) or anti-rabbit antibody (A11037) from Molecular Probes for immuno-fluorescence ...