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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for African Swine Fever Virus P72 Protein His tag E. coli since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: ... Proteins were expressed in Escherichia coli strain BL21(DE3)pLysS (Thermo Fisher Scientific). 1-4 liters of cells were grown in Luria-Bertani (LB ...
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bioRxiv - Immunology 2023Quote: ... Expression constructs for M35-V5/His and M27-V5/His (both in pcDNA3.1-V5/His, Invitrogen) have been described previously (Munks et al ...
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bioRxiv - Immunology 2023Quote: ... Media containing virus particles was first concentrated to 20x using 100K MWCO protein concentrator columns (Thermofisher). Concentrated virus was then incubated using 1:2000 DiD-Cell labelling solution (ThermoFisher V22887 ...
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bioRxiv - Cell Biology 2019Quote: ... The His-mCherry-fusion proteins were purified using a Ni-NTA column (Thermo Fisher Scientific). The eluted proteins were dialyzed in 1 L dialyzed buffer (20 mM Tris-HCl pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... His-tagged proteins were visualised using a primary anti-6xhis antibody (ArtNr: 11533923, Fisher Scientific), a HRP-coupled secondary antibody (ArtNr ...
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bioRxiv - Neuroscience 2023Quote: ... The His-tagged dAsapPZA protein was expressed and purified following the manufacturer’s guidelines (Invitrogen, USA).
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bioRxiv - Bioengineering 2021Quote: The HHH peptide (Cys-biotin-PEG2-Gly-His-His-His-Gly(COOH)) was purchased from Thermo Fisher. It was 84% pure as determined by mass spectrometry ...
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bioRxiv - Biochemistry 2020Quote: ... The gene optimized for the Drosophila codon usage and extended by C-terminal His-tag was purchased from Life Technologies (DNA String fragments, USA). The DNA fragment was inserted into a pMT-BiP-V5-His_A vector (ThermoFisher ...
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bioRxiv - Cell Biology 2022Quote: 1-2 μg of purified 6xHis-tagged calcineurin or 6xHis-MBP as a negative control was first bound to 10 μL magnetic Dynabeads™ His-Tag Isolation and Pulldown Beads (Thermo Fisher Sci., 10104D) in 490 μL base buffer (50 mM Tris-HCl pH 7.5,150 mM NaCl ...
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bioRxiv - Molecular Biology 2019Quote: African green monkey epithelial cells (Vero) were grown in Dulbecco’s modified Eagle’s medium (DMEM) (Life Technologies), containing 100 units/ml penicillin ...
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bioRxiv - Microbiology 2019Quote: ... VeroE6 (African green monkey kidney) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2021Quote: African green monkey kidney epithelial VeroE6 (ATCC) were grown in Dulbecco’s Modified Eagle’s Medium (DMEM, Thermofisher, supplemented with 1% Non-Essential Amino-Acids (Thermofisher) ...
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bioRxiv - Microbiology 2020Quote: ... Vero cells (African green monkey kidney cell) were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM, Gibco) supplemented with 10% FBS.
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bioRxiv - Microbiology 2022Quote: African green monkey cells (Vero E6) were grown in Dulbecco’s modified Eagle’s medium (DMEM 1X, GIBCO) supplemented with 5% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2023Quote: ... HEK293T human embryonic kidney cells and Vero African Green monkey cells were maintained in DMEM (Gibco) containing 10% (v/v ...
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bioRxiv - Microbiology 2024Quote: ... African green monkey kidney epithelial (Vero; ATCC; CCL-81) cell line was maintained in DMEM (Gibco) supplemented with 10% FBS and cultured at 37 °C with 5 % CO2 ...
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bioRxiv - Immunology 2022Quote: Preparation of CD5L-coated pHrodo particles: one vial containing 2 mg of particles (pHrodo™ Red E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher) were reconstituted with 500 μL of TBS with 5 mM CaCL2 and sonicated for 5 min ...
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bioRxiv - Biochemistry 2023Quote: ... Digested scFv inserts were subcloned in pET22b(+) vector (EMD Biosciences) and transformed into BL21(DE3) E-coli strain (Thermo Fisher Scientific, USA). Amber stop codon in CDR regions of scFvs were mutated to glutamine by site-directed mutagenesis kit (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... Cdc50 proteins fused to the 10xHis tag were detected using the HisProbeTM-HRP conjugate (ThermoFisher) diluted to 1:2000 in 2 % (w/v ...
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bioRxiv - Biochemistry 2019Quote: ... The GST tag of the VP8* protein was removed using the thrombin (Thermo Fisher Scientific) after dialysis into the buffer (20 mM Tris-HCl ...
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bioRxiv - Microbiology 2020Quote: ... as measured on protein level (BCA protein concentration determination kit; before digestion) was labeled with 10-plexing tandem mass tags (TMT-10; Thermo Scientific) following the manufactures instructions ...
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bioRxiv - Neuroscience 2019Quote: ... TAG concentrations were normalized to the total protein content as determined by a Micro BCA protein assay kit (ThermoFisher, Cat # 23235). Each assay was done in triplicate and the average TAG level (μg TAG/ μg protein ...
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bioRxiv - Cancer Biology 2022Quote: ... (FBS HI; Invitrogen) and 1% Glutamax (Invitrogen) ...
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bioRxiv - Microbiology 2022Quote: ... anti-His (Invitrogen), or anti-FLAG (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Microbiology 2022Quote: ... V5 tag to detect CBUA0028 or the Xpress tag (Invitrogen) to detect CBU0665.
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bioRxiv - Microbiology 2019Quote: ... coli protein production was performed using the pET-SUMO (Thermo Fisher, Waltham, MA, USA) expression system in E ...
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bioRxiv - Molecular Biology 2019Quote: 6xHis 12-heptad SRS fusion proteins were expressed in SoluBL21 Escherichia coli cells (Invitrogen) from a pet42a vector ...
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bioRxiv - Evolutionary Biology 2022Quote: For protein purification Escherichia coli strains were tested for optimal expression: BL21 DE3 (Thermofisher) was selected for PpMurE_L63_pPROEX and BL21(DE3) ...
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bioRxiv - Microbiology 2019Quote: ... Strains used for cloning and expression of recombinant proteins were Escherichia coli TOP10 (Invitrogen) and E ...
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bioRxiv - Cell Biology 2021Quote: ... coli (K-12 strain), and Staphylococcus aureus (Wood strain, without protein A) (Molecular Probes), were washed 3 times with PBS by centrifugation and sonicated for 3 times at 50 KHz for 20 s ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Immunology 2020Quote: Recombinant soluble ZIKV E protein was biotinylated and coupled to AF488 Neutravidin beads (Life Technologies). Microscale-purified mAbs were tested at a single 1:10 dilution ...
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bioRxiv - Cell Biology 2021Quote: ... HA-tag (Invitrogen), Flag-tag (Sigma) ...
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bioRxiv - Biophysics 2021Quote: ... The SNAP-tag allows for covalent binding of O6-benzylguanine (BG) to the protein.[63] Protein expression was performed in Escherichia coli BL21(DE3) pRARE (Invitrogen) under T7 promoter with 1 mM isopropyl-β-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Microbiology 2021Quote: ... Standard Western blotting procedures were used with the following antibodies: HIV-1 gp120 (NIH AIDS Reagent program catalog no. 288) and 6x-His Tag (ThermoFisher catalog no. MA1-21315-HRP).
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Synthetic Biology 2021Quote: ... Sybr Safe DNA gel stain and BenchMark™ His-tagged Protein Standard were purchased from Invitrogen. HRP-conjugated 6*His His-Tag Mouse McAB was obtained from Proteintech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... His-tagged proteins were eluted from the beads in a disposable polypropylene column (29924, ThermoFisher Scientific) using elution buffer (4 ml wash buffer containing 400 mM imidazole ...
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bioRxiv - Genetics 2019Quote: ... The His-ChpBK fusion protein was purified from clarified lysates using a Ni-NTA beads (Invitrogen). Protein purity was determined by SDS-PAGE analysis and samples were quantified using the Bio-Rad protein assay and stored at −80 □.
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bioRxiv - Neuroscience 2020Quote: ... media was collected and His tagged proteins were bound using HisPur Ni-NTA Resin (ThermoFisher; 88221) in the presence of Calbiochem’s EDTA-free protease inhibitor cocktail 1:1000 (MilliporeSigma ...
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bioRxiv - Immunology 2020Quote: RBD-His protein was expressed in Expi293 cells and purified on Ni-NTA resin (#88221, Thermofisher) followed by size-exclusion chromatography on a Superdex 200 gel filtration column in PBS 13 ...
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bioRxiv - Cell Biology 2023Quote: ... The in vitro-translated proteins were then purified using His Pur cobalt spin columns (Thermo Scientific). For in vitro kinase assays ...
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bioRxiv - Neuroscience 2023Quote: ... The purified His-TEV-FAM19A5 proteins were digested overnight at 30°C with AcTEV protease (Invitrogen) to remove the His-tag and TEV sequence ...
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bioRxiv - Synthetic Biology 2022Quote: ... Cloning inserts were created via PCR of ORFs of interest from their respective genomic (all E. coli genes) or codon-optimized (all other genes) DNA with Phusion polymerase (Thermo Scientific, Waltham, MA). The resulting In-Fusion products were used to transform E ...
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bioRxiv - Neuroscience 2020Quote: ... cells were washed with PBS and incubated with pHrodo-labeled bioparticles (pHrodo™ Green E. coli BioParticles™ Conjugate for Phagocytosis, ThermoFisher Scientific) in OptiMem for three hours at +37°C ...
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bioRxiv - Microbiology 2020Quote: ... according to manufacturer’s instructions with primers purchased from Eurofins Genomics. E. coli DH5α and Stbl2 (Trinh et al., 1994, p. 2) (Thermo Fisher Scientific, USA) were used for amplification of standard plasmids or LTR containing plasmids ...
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bioRxiv - Bioengineering 2022Quote: ... bacteria were captured using commercially available beads with their associated magnetic separator device (anti-E. coli O157 Dynabeads®, DynaMag™ -2 magnet, Life Technologies) following the manufacturer’s guidelines for use ...
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney (Vero) cells were maintained in Dulbecco’s Modified Eagle Medium (DMEM, Life Technologies™). Both culture media were supplemented with 10% HyClone® standard fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2021Quote: Vero (African green monkey kidney) cells (ATCC CCL81) were maintained in Dulbecco’s modified minimal medium (Life Technologies) with 10% fetal bovine serum (Atlanta Biologicals ...