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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for Adenovirus Type 5 Particles CMV Luciferase since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2019Quote: ... immortalized wild type MEFs (Heale et al., 2006) and 293T cells were cultured at 37°C and 5% CO2 in DMEM (Gibco 31600-034) supplemented with 10% fetal bovine serum and penicillin-streptomycin (50U/mL).
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bioRxiv - Cancer Biology 2020Quote: ... Then 100 mg of tumor was collected and isolated in single cells by the buffer containing 5 mg/ml collagenase type IV (Gibco, 17104-019) and 1 mg/ml dispase II (Gibco ...
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bioRxiv - Neuroscience 2023Quote: Primary microglial cultures were prepared using whole brains of P0-P1 C57BL/6J wild-type or SorLA-KO newborns as described (5) and cultured in DMEM w/Glutamax (Gibco, #10569-010) with 10% FBS (Pan Biotech ...
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bioRxiv - Microbiology 2020Quote: ... The viral particle concentration was determined by Qubit fluorometric quantification (ThermoFisher) against a DNA standard (1 µg = 2.34E+10 virions) ...
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bioRxiv - Biochemistry 2019Quote: ... 3 µm particle size (Thermo Fisher Scientific Inc., Waltham, MA U.S.A.) and separated using a 130 min gradient (100 min from 0% −35% Buffer B ...
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bioRxiv - Immunology 2021Quote: ... Virus particles in 2% FBS/DMEM/1mM Pen/Strep (Thermofisher, 15140122) were incubated with cells for 2 hours at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells and purified virus particles were lysed with RIPA (ThermoFisher Scientific) buffer supplemented with complete protease inhibitor cocktail (Roche ...
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bioRxiv - Microbiology 2022Quote: ... Equal MV particles were labelled with R18 (R18, ThermoFisher Scientific O246) and bound to HeLa cells or blebs and the pH maintained at 7.4 or lowered to 5.0 by the addition of 100 mM MES. ...
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bioRxiv - Microbiology 2023Quote: ... on the KingFisher Flex Magnetic Particle Processor (ThermoFisher Scientific, Waltham, MA), according to manufacturer’s protocols ...
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bioRxiv - Cancer Biology 2023Quote: Lentiviral particles were produced by transfecting 293FT cells (Thermo Fisher, R70007) with the pBA439_UMI20 library ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 µl of Dynabeads Protein G magnetic particles (Thermo Fisher Scientific) per ml of total lysate were washed twice with 1 mL of PBS-Tween (0.02%) ...
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bioRxiv - Molecular Biology 2023Quote: Virus particles were fixed in 1 % glutaraldehyde (Cat# 233281000, Thermo Scientific). A 4 µL aliquot of sample was adsorbed onto holey carbon-coated grid (Lacey ...
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bioRxiv - Biochemistry 2024Quote: ... column (Betasil C18, 100 × 2.1 mm, 3 µm particle; Thermo Scientific) was washed with 100 mL of Solvent B (MeOH ...
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bioRxiv - Microbiology 2023Quote: ... and hydrophobic (Sera-Mag Speedbead carboxylated-modified particles, Thermo Fisher Scientific) magnetic beads ...
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bioRxiv - Bioengineering 2023Quote: ... 2.4 μm particle size (Thermo Scientific, Waltham, MA, USA, 28102–152130). A mobile phase of A ...
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bioRxiv - Cell Biology 2020Quote: ... and 0.1% type II collagenase (Invitrogen) in Dulbecco’s modified Eagles medium (DMEM ...
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bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2022Quote: ... (Type IV, Thermo Fisher Scientific, 17104019) and 0.05% Trypsine EDTA (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... 1.2mg/mL collagenase type II (Gibco), and calcium chloride (Sigma ...
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bioRxiv - Bioengineering 2021Quote: ... Type I agarose (Fisher scientific, UK) was added to distilled water and autoclaved at 121 °C to make 2% ...
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bioRxiv - Immunology 2020Quote: ... Type IV Collagenase was from Gibco. DNase I was from Applichem ...
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bioRxiv - Physiology 2019Quote: ... containing 0.2% type II collagenase (Gibco) for one hour at 37°C with constant agitation ...
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bioRxiv - Immunology 2022Quote: ... 37.5 mg collagenase type II (Gibco), 1 mL PBS ...
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bioRxiv - Neuroscience 2023Quote: ... and Dispase type II (ThermoFisher Scientific) for 15 minutes at 37°C ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.2mg/mL collagenase type II (Gibco), and calcium chloride (Sigma ...
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bioRxiv - Immunology 2024Quote: ... 37.5 mg collagenase type II (Gibco), 1 mL PBS ...
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bioRxiv - Cell Biology 2021Quote: ... a luciferase-based assay (ATP Determination kit, Invitrogen) was used ...
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bioRxiv - Cell Biology 2019Quote: All ORFs were cloned into pHAGE-CMV-FLAG via LR clonase (Thermo Fisher, 11791-020): pDONOR223.1 BRSK2 (splice isoform 3 ...
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bioRxiv - Physiology 2019Quote: Recombinant adenoviruses were generated using the pAd/CMV/V5-DEST Gateway recombination system (Life Technologies) after cloning the full-length cDNA into the pENTR vector ...
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bioRxiv - Microbiology 2019Quote: ... All genes of interest were cloned into pAd/CMV/V5-DEST by Gateway recombination (Invitrogen). The completed vectors were confirmed by DNA sequencing ...
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bioRxiv - Cell Biology 2023Quote: ... The modified pGL4.13 plasmids and pRL-CMV plasmid were co-transfected with Lipofectamine 2000 (Invitrogen) at a mass ratio of 30:1 ...
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bioRxiv - Biochemistry 2024Quote: ... or FLAG:ACAD11 expressed under a CMV promoter in pcDNA3.1 constructs using Lipofectamine 3000 (Thermo Scientific) in OptiMEM (Gibco) ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 ng of PGL4 (hRluc-CMV) plasmid using lipofectamine-3000 as transfection reagent (Invitrogen). For RU486 treatment ...
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bioRxiv - Bioengineering 2020Quote: ... luminescence was compared to a ladder of recombinant Gaussia Luciferase (NanoLight #321-500) quantified using a Gaussia Luciferase Glow Assay Kit (ThermoFisher #16161) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2022Quote: ... The luciferase constructs and Firefly luciferase mRNA were in vitro transcribed using the Sp6 mMessage machine kit (Ambion Inc, Austin, Texas). In vitro transcribed mRNAs were purified using Macherey-Nagel Nucleospin® RNA Clean-up kit (Macherey-Nagel ...
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bioRxiv - Molecular Biology 2024Quote: Luciferase emissions from transfected Hek-Luc cells were measured using the Pierce® Firefly Luciferase Glow Assay Kit (Thermo Fisher Scientific). To initiate cell lysis ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... was co-transfected with 5 μg tk-(MH100)4-luciferase reporter and 5 μg CMX-β-galactosidase transfection control plasmids in Opti-MEM using Lipofectamine 2000 reagent (Invitrogen Life Technologies, Grand Island, NY), following the manufacturer’s recommended protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... The seminiferous tubules were digested in 5 mL of DPBS solution I consisting of 1 mg/mL collagenase type IV (17104019, Gibco, Grand Island, NY) and 20 U/mL DNase I (2212 ...
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bioRxiv - Cancer Biology 2020Quote: ... using lysates from cultured cells that were co-transfected with firefly luciferase and internal control Renilla luciferase plasmids using Lipofactamine 2000 (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... the medium is removed and 50ng of the gaussia luciferase sensors mRNA mixed with the 50ng of cypridina luciferase mRNA in Lipofectamine MessengerMax (Thermo Fisher Scientific) following manufacturer’s specifications and transfected into each well.
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bioRxiv - Microbiology 2022Quote: ... then samples were stored at -80°C until measuring Gaussia luciferase activity using the Gaussia Luciferase Flash Assay Kit (ThermoFisher Scientific, 16158) as per the manufacturer’s directions on a FLUOstar Omega microplate reader (BMG Labtech) ...
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bioRxiv - Immunology 2023Quote: ... cells were collected at 24 h post-transfection and luciferase activity was analyzed by Dual-Luciferase Reporter Assay Kit (Vazyme) performed with a Luminoskan Ascent luminometer (Thermo Fisher Scientific). The activity of Firefly luciferase was normalized by that of Renilla luciferase to obtain relative luciferase activity.
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bioRxiv - Molecular Biology 2021Quote: ... and then purified by Sera-Mag Magnetic Streptavidin-coated particles (Thermo Scientific).
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bioRxiv - Microbiology 2020Quote: ... that is run on a Kingfisher-96 magnetic particle handling system (ThermoFisher). After purification the nucleic acid was eluted in 50uL of kit elution buffer and 5uL run in the appropriate qRT-PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... and pressure loaded with Poros R2-C18 10 μm particles (Life Technologies). The analytical column consisted of a 25 cm × 75 μm internal diameter column with an integrated electrospray emitter (New Objective) ...
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bioRxiv - Microbiology 2020Quote: ... RNA was isolated from benzonase-treated particles using TRIzol LS Reagent (Invitrogen), according to manufacturer’s protocol.
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bioRxiv - Microbiology 2020Quote: ... the virus particles were resuspended in CO2-independent media (ThermoFisher, Cat# 18045088), aliquoted ...
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bioRxiv - Molecular Biology 2022Quote: ... 50 μm inner diameter and 2 μm particle size (Thermo Fisher Scientific). The mobile phase flow rate was 300 nl/min using 0.1% formic acid in water (solvent A ...