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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 9 10 Dihydro 4H benzo 4 5 cyclohepta 1 2 b thiophen 4 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Biochemistry 2020Quote: ... Each well was washed with PBS and then incubated for one hour at 4°C while rocking with 1 mg/mL EZ-Link NHS-SS-Biotin (Thermo Fisher Scientific) in PBS ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were washed 2-3 times (200 rpm, 5 min at 4°C) in eBioscience™ Permeabilization buffer (250 µl/well) (Invitrogen) and resuspended in eBioscience™ Fixation/Permeabilization solution (Invitrogen ...
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bioRxiv - Genetics 2022Quote: ... Ly6Chi monocytes were labeled by intraperitoneal injection of 4 mg/mL of Edu (5-ethynyl-2’-deoxyuridine) from Life technologies (NY), and mice were euthanized after 5 days to assess baseline recruitment or 21 days to assess for retention ...
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bioRxiv - Genetics 2020Quote: ... boiled in reducing sample buffer for 5 min and resolved on 4–12% Bis-Tris Bolt gels and transferred using an iBlot 2 (Thermo Fisher). Blots were blocked in 2.5% milk in 1% TBS-Tween before staining with antibodies (Table S5) ...
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bioRxiv - Synthetic Biology 2021Quote: ... cells were dissociated into single cells with TrypLETM for 4-5 minutes at 37 C and seeded into 2 % Geltrex coated 96- or 12-well plates (Fisher Scientific) in Essential 8TM Medium (Gibco ...
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bioRxiv - Microbiology 2022Quote: ... A 3% agarose gel with 4 μL ethidium bromide was loaded with 5 μL PCR reaction and 2 μL GeneRuler 100bp ladder Plus (Thermo Fisher) and run for 40 minutes at 100 volts ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Microbiology 2023Quote: ... 20% volume of 1M 2-morpholin-4-ylethanesulfonic acid (MES) pH 5 and Immobilized Protein G resin (Thermo Scientific Prod#20397) were added to supernatant and sample was incubated overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... We mixed an equal volume of the extracted LPS samples with an equal volume of 2× SDS buffer and electrophoresed 10 µl of each sample on Novex 4-20% plyacrylamide gradient gels (ThermoFisher) in Tris-Glycine-SDS buffer ...
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bioRxiv - Biochemistry 2022Quote: ... at a concentration of 2 x 105 cells per ml (4 ml per dish hereafter) in DMEM (Nissui Pharmaceutical) supplemented with 10% FBS (Gibco), glutamine ...
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bioRxiv - Molecular Biology 2022Quote: ... Lysates were centrifuged at 17,000 × g for 10 min and supernatant incubated overnight at 4 °C with 2 μg of anti-PTBP1 antibody (monoclonal-ThermoFisher) or anti-FLAG antibody (monoclonal-Sigma) ...
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bioRxiv - Systems Biology 2022Quote: ... Transcriptomics using the isolated mRNA from liver tissues (0, 2, 4, 6, 8 and 10 weeks; n=3 per time point) was performed by Affymetrix GeneChip®Mouse Gene 2.0 ST Arrays (902118) ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Biochemistry 2023Quote: ... and 10% 2-Mercaptoethanol or 50 mM dithiothreitol (DTT) and run on NuPAGE 4-12% bis-tris gels (Thermo Fisher). Proteins were transferred to nitrocellulose membranes ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... and Nuclear DNA was counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher). Alternatively ...
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bioRxiv - Biophysics 2020Quote: ... Nuclei were detected by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:200, ThermoFisher Scientific, USA) for 10 minutes at RT ...
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bioRxiv - Bioengineering 2021Quote: ... buffered with 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
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bioRxiv - Neuroscience 2020Quote: ... sections were counterstained with 4’,6-diamidine-2’-phenylindole dihydrochloride (DAPI, D3571, Molecular Probes, dilution 1:1000), re-washed ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... stock solutions were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The thrombin-selective inhibitor PPACK.2HCl and MMP Inhibitor V (ONO-4817 ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were counterstained with 4’,6-diamino-2-phenylindole dihydrochloride (DAPI, 1:10000 in PBS, Thermo Scientific) and mounted with a cover glass using Fluoromount (Diagnostic BioSystems ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were visualized with Hoechst (4′,6-diamidino-2-phenylindole) (DAPI) (Invitrogen; 3258, ICC/IHC 1:5,000).
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bioRxiv - Physiology 2022Quote: ... Nuclei were stained with 4′,6-diamidino-2-phenylindole (1:2500, DAPI, ThermoFisher Scientific, Cat No. D1306). Images were taken using Zeiss 880 confocal microscopy and quantified by using Fiji/ImageJ86.
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bioRxiv - Neuroscience 2023Quote: ... cell nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, Thermo Fisher Scientific, 1:5000 dilution) for 15 mins ...
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bioRxiv - Biochemistry 2023Quote: ... The slides were then incubated with 1 ug/ml of 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen) in 1xPBS for 20 min at RT ...
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bioRxiv - Developmental Biology 2024Quote: ... Antibodies were used together with DAPI (4’,6-diamidino-2-phenylindole, Thermo Scientific, 62248, 1:1,000 dilution). NDE1 (Abnova ...
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bioRxiv - Developmental Biology 2024Quote: Ovaries from 5-7 females were dissected in 1% PBS-Tween and fixed in a 1:1 mixture of 4% formaldehyde (Thermo Scientific, 28908) and heptane (Sigma Aldrich ...
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bioRxiv - Bioengineering 2023Quote: ... All samples were then incubated with 4′,6-Diamidino-2-phenylindole (DAPI, 2 µM, Thermofisher) as a nuclear counterstain and Alexafluor 555 phalloidin (1:60 ...
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bioRxiv - Cell Biology 2020Quote: ... Cultured colonies from organoids were fixed with 4% PFA for 2-4 hrs at room temperature followed by immobilization with Histogel (Thermo Scientific) for paraffin embedding ...
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bioRxiv - Biophysics 2023Quote: ... Samples were mildly fixed with 4% PFA for 4 min before immunostaining using anti SARS-CoV-2 Spike Protein S1 (Cat# PA5-114447, ThermoFisher Scientific) and anti-human IgG (Fc)-Alexa 488 (Cat# 109-545-170 ...
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bioRxiv - Cancer Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 mm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive ...
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bioRxiv - Systems Biology 2023Quote: ... with a Dionex IonPac AS11-HC-4 μm column (2 μm i.d. × 250 mm, 4 μm particle size, Thermo Fisher Scientific) coupled with a Q Exactive high-performance benchtop quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... Invitrogen E-Gel EX Agarose Gels 4%, and DAPI (4’,6-Diamidino-2-Phenylindole, Dihydrochloride, D1306) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... with 4 μg of a plasmid mixture (1:1:4 THR:RXR:TRE-Luc) in 200 μL Opti-MEM (Invitrogen, 11058-021) and added to the cells ...
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bioRxiv - Physiology 2021Quote: ... 10% Amphotericin B (15290026, Gibco) and 1X REGMTM SingleQuotsTM kit (CC-4127 ...
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bioRxiv - Biochemistry 2020Quote: ... cells were incubated with an alpha tubulin monoclonal antibody conjugated to Alexa Fluor 488 (B-5-1-2, Invitrogen, Carlsbad, CA) at 2 μg/mL in 1% BSA in PBS for 1 hour ...
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bioRxiv - Molecular Biology 2022Quote: ... siM1-4: 5’ -ACTGGTAGCTTATTAAAGATT- 3’) and the HOXA1 siRNA (5’ - AGAACTTCAGTGCGCCTTATT- 3’) were purchased from Invitrogen™ (Silencer® Select siRNA ...
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bioRxiv - Biochemistry 2019Quote: ... 4°C and lysed using 400 µL B-PER bacterial protein extraction reagent (Thermo Scientific, MA, USA) according to the instructions of the manufacturer ...
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bioRxiv - Molecular Biology 2020Quote: ... siRNA (4): s35234 and siRNA (5): s35235 (Silencer Select, ThermoFisher Scientific, USA); EMC2 s18670 and EMC5 s41131 (Silencer Select ...
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bioRxiv - Microbiology 2019Quote: ... The membrane was stained with 5 μg/ml FM 4-64FX (Invitrogen) fluorescent dye while the nucleoid was stained with 2 μg/ml DAPI (Millipore Sigma ...
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bioRxiv - Systems Biology 2019Quote: ... the reaction was quenched with 4 μL 5% hydroxylamine (Thermo Scientific, 90115) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... 2.5-5 µg was loaded onto a NuPAGE 4-12% gel (ThermoFisher) and run at 100V for 2-3 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and passaged every 4-5 days using StemPro Accutase (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... 5 ng/ml IL-4 (Thermo Fisher Scientific, Cat#14-8041-62) and 1 ng/ml TGF-β1 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2023Quote: Samples were loaded with 5 μM Fluo-4 AM (ThermoFisher Scientific, Germany) in CM for 30 min in a cell culture incubator (37 °C and humidified 5% CO2) ...