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Citations for Thermo Fisher :
551 - 600 of 4948 citations for 9 Trimethylsilyl 6 trimethylsilyl oxy 9H purine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: Cells were lysed with 1X NUPAGE lysis buffer containing 1X HaltTM protease inhibitor (Thermo Scientific, 18612793+9), 1X phosphatase inhibitor cocktail 2 (Sigma-Aldrich ...
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Antimicrobial and antibiofilm activity of human recombinant H1 histones against bacterial infectionsbioRxiv - Microbiology 2024Quote: ... and stained with Syto 9 and Propidium iodide (Live/Dead BacLight Bacterial Viability Kit, Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: Primary CLL cells were cultured for up to 9 days in Iscove’s Modified Dulbecco’s Medium (IMDM; Gibco) supplemented with 10% Fetal bovine serum (Gibco) ...
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bioRxiv - Microbiology 2024Quote: ... The biofilm was fixed with 4% paraformaldehyde and stained with SYTO 9 fluorescent dye (Thermo Fisher Scientific). Z-stack images were captured on the Olympus FluoView FV1200 confocal microscope ...
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bioRxiv - Cancer Biology 2019Quote: ... 300,000/6-well) and IDC (5,000/96-well; 100,000/6-well) cells were seeded in regular (Thermo Fisher Scientific) or ULA (Corning Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were forward transfected in 6-well dishes by using 6 μl of FuGENE® HD (PRE2311, Fisher Scientific) with 3 μg of sgRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2020Quote: Cells in the 6-well plates were transfected with 2μg plasmid of AMPKα or OGG1 via 6 μl Lipofectamine 2000 (Invitrogen) for 6 hours following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The Cas9-sgRNA-containing plasmid and donor vector were co-transfected at a molar ratio of 1:6 into XX mESCs cells on a 6-well plate using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher). Transfected cells were passaged at different densities into three Petri dishes with feeders ...
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bioRxiv - Biochemistry 2021Quote: ... 6% native PAGE gels were prepared by first making a gel solution containing 6% acrylamide (29:1) (Fisher Scientific).15 This solution was then degassed under vacuum and mixing ...
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bioRxiv - Developmental Biology 2020Quote: Skin tissues collected from newborn Foxn1−/− (Cby.Cg-Foxn1
/cmdb) and Foxn1+/+ (C57BL/6; B6) mice were incubated in dispase (6 U/ml; Life Technologies) overnight at 4°C ... -
bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
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bioRxiv - Molecular Biology 2020Quote: ... 2:6 and 3:6 dilution ratios to allow efficient selection of Hygromycin B (Thermo Fisher Scientific Catalog Number: 10687010). The Hygromycin selection was started at the 48 hours after transfection time point with a final concentration of 150µg/ml and refreshed every 3-4 days until the control non-transfected cells on a separate plate were completely dead (takes approximately 3 weeks from the start of transfection until the cells are expanded and frozen) ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated without ligand or with EGF (6 μM) or EREG (6 μM) in buffer A containing disuccinimidyl suberate (DSS) at 62.5 μM (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: 293T-ACE2 and HT1080-ACE2 cells were seeded in 24-well plates and 6-well plates respectively to achieve 70% confluency after 4-6 hours and then transfected with Lipofectamine RNAiMAX (Thermofisher) using the indicated dsiRNAs (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transiently transfected in 6-well plates with 6 individual IFNLR1-gRNA plasmids using Lipofectamine 3000 (Life Technologies). The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... treated wild-type C57BL/6 and 12-week-old Rad1 CKO mice were subjected to phosphopeptide enrichment and 6-plex TMT (ThermoFisher) labeling (Sims et al. ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit ...
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bioRxiv - Immunology 2023Quote: ... The recombinant bacmid DNA was transfected into the Sf9 insect cells seeded in 6-well plates at 0.5 × 106 cells/ml using Fugene 6 reagent (Invitrogen protocol). At 72 hours post-transfection ...
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bioRxiv - Microbiology 2023Quote: Interleukin-6 (IL-6)_ and interleukin-12 (IL-12_ expression levels were detected by ELISA Kits (Thermo Fisher Scientific, USA); the samples analysed via ELISA were either plasma drawn from mice or cell culture supernatants ...
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bioRxiv - Immunology 2024Quote: ... primary Rhinolophus ferrumequinum bat cells were seeded in 6-well plate at a density of 6×105 cells/well with DMEM containing 10% FBS (Gibco). After 12 h ...
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bioRxiv - Microbiology 2020Quote: ... actively growing Zm6 culture was incubated with SYTO™ 9 Green Fluorescent Nucleic Acid Stain (Thermo Fisher Scientific) at a concentration of 0.5 or 11 μM for 10 minutes ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split every 2-3 days through 9 minutes incubation with EDTA 50mM pH=7.4 and 3 minutes of 0.25% trypsin (Gibco).
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bioRxiv - Immunology 2022Quote: ... was used to dissociate cells before screening or sorting with anti-CD58 (TS2/9, Invitrogen 12-0578-42) or anti-ICAM1 (HA58 ...
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bioRxiv - Immunology 2020Quote: ... Tetramers were prepared in house by mixing biotinylated (NANP)9 peptide with streptavidin conjugated PE or APC (Invitrogen) in a 4:1 molar ratio ...
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bioRxiv - Biochemistry 2020Quote: ... The mixture was then brought to 40 °C and 9 g of tetraethyl orthosilicate (98%, ACROS Organics #1577812500) was added ...
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bioRxiv - Bioengineering 2020Quote: ... This segmented data was then exported as a binary ROI and imported into the Avizo 9 software (ThermoFisher) for visualization ...
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bioRxiv - Cell Biology 2021Quote: ... sqh-GFP. Ovaries were dissected in Stage 9 (S9) medium (Prasad et al. 2007): Schneider’s medium (Life Technologies), 0.6x penicillin/streptomycin (Life Technologies) ...
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bioRxiv - Biophysics 2021Quote: HEK293T cells (9×106 per 15 cm dish) were plated in antibiotic free DMEM (Gibco, Cat. No. 11995073) supplemented with 10%FBS (Gemini bio-products ...
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bioRxiv - Immunology 2022Quote: Mast cell line MC/9 was purchased from ATCC(CRL-8306) cultured in Dulbecco’s Modified Eagle’s Medium(GIBCO, Invitrogen) adjusted to contain 4 mM L-glutamine ...
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bioRxiv - Microbiology 2020Quote: ... colonies were stained using 500 µl of GC medium containing 3 µmol/l Syto 9 (Thermo Fisher Scientific) and 4.5 µmol/l propidium iodide (PI ...
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bioRxiv - Microbiology 2020Quote: ... the cells were grown from day 0 to day 9 in RPMI 1640/GlutaMAX medium (ThermoFisher Scientific; #61870036) containing the insulin-free B-27 supplement (ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: Human bladder carcinoma 5637 cells (ATCC, HTB-9) were grown in RPMI-1640 media (GIBCO, cat.no. 21875-034) containing 10% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2019Quote: ... 10,000 cells/cm2 were seeded in triplicate Nunc™ 9 cm2 Slideflasks (Thermo Fischer Scientific Nunc A/S). Control slideflasks were included by seeding 5500 cells/cm2 in expansion media in absence of adipogenic stimulation ...
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bioRxiv - Cell Biology 2021Quote: ... cells were first imaged every 9 seconds before dextran (Texas Red™ 70,000 MW Lysine Fixable, Invitrogen, #D1864) was added at 50μg/ml mid-imaging.
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bioRxiv - Neuroscience 2021Quote: ImM10 immortalized murine MG cells (35) (passage 9-14) were kept in ImM10 Media [Neurobasal Media (Gibco #21103049), 1x Antibiotic-Antimicrobial (Gibco #15240-062) ...
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bioRxiv - Biochemistry 2021Quote: ... Images of 9 fields per well were obtained using the CellInsight CX5 High-Content Screening Platform (ThermoFisher Scientific) with a 10X objective ...
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bioRxiv - Cancer Biology 2022Quote: Bottom layer agar was made to reach a final concentration of 0.8% agar (DF0812-17-9; Fisher Scientific), 0.2% peptone (DF0118-17-0 ...
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bioRxiv - Bioengineering 2022Quote: ... Silk was dried and subsequently dissolved in 9 M LiBr (199870025, Acros, Thermo Fisher Scientific, Breda, The Netherlands), filtered ...
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bioRxiv - Developmental Biology 2022Quote: ... containing 5 mL of digestion buffer [composed of 9 mL of phosphate-buffered saline (PBS; Gibco, 10010-023) combined with 1 mL of Dispase (stock ...
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bioRxiv - Microbiology 2022Quote: ... envelope plasmid (0.9 µg pMD2.G) and pLX304 carrying eGFP-LC3B (9 µg) using Opti-MEM (Fisher Scientific) and FuGENE HD transfection reagent (Promega) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (9×106 per 15 cm dish) were plated in antibiotic free DMEM (Gibco, Cat. No. 11995073) supplemented with 10%FBS (Gemini bio-products ...
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bioRxiv - Cell Biology 2023Quote: ... Three custom stealth small interfering RNA (siRNA) were used to silence galectin-9 (LGALS9HSS142807, LGALS9HSS142808 and LGALS9HSS142809) (Invitrogen). Equal amounts of the siRNA ON-TARGETplus non-targeting (NT ...