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Citations for Thermo Fisher :
151 - 200 of 10000+ citations for 8 bromo 4 methoxyquinoline since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Microbiology 2022Quote: ... mutations were inserted in fragment 8 (Supplementary Table 4) using the GENEART site-directed mutagenesis system (Thermo Fisher Scientific, Cat# A13312) according to the manufacturer’s protocol with the primers listed in Supplementary Table 5 ...
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bioRxiv - Systems Biology 2022Quote: Cells were cultured and transfected (as described above) for CLSM experiments on 4 or 8-well Lab-Tek™ chambered cover glass slides (ThermoFisher). Before imaging ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Evolutionary Biology 2020Quote: ... The PCR products were analyzed by using an 8 capillary ABI 3500 DNA Genetic Analyzer with POP-4 polymer (Life Technologies) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Evolutionary Biology 2020Quote: ... We extracted RNA from sets of 8 adult males (2~4 day old) in triplicate from each RNAi cross using TRIzol (Catalog# 15596-026, Invitrogen, USA), treated ~2 μg RNA with RNase-free DNase I (Catalog# M0303S NEW ENGLAND Biolabs ...
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bioRxiv - Immunology 2020Quote: 7575 naïve CD4 cells were isolated from lymph nodes of CCT8T+/+ and CCT8T−/− mice at 4-8 weeks of age by FACS sorting directly into lysis buffer (RIPA buffer (ThermoFisher Scientific) + 4 % IGEPAL CA-360 (Merck) ...
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bioRxiv - Immunology 2022Quote: ... Cell debris was removed from lysate containing soluble protein by centrifugation at 35,000 x g and 4 °C for 45 min (Sorvall LYNX 6000 centrifuge, F21-8×50y rotor, Thermo Fisher Scientific). Proteins were further purified by immobilized metal affinity chromatography (IMAC ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Immunology 2023Quote: ... Vesicles were subjected to high-speed centrifugation for 4 h at 55,000 g (25°C) and subsequently at 8 h at 75,000 g (4°C) with an ultracentrifuge (Sorvall RC M150GX, Thermo Fisher Scientific) using a fixed angle rotor (S150AT-0128 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation (19 000 rpm for 45 min at 4°C in a Fiberlite F21-8×50y (Thermo Scientific). Supernatant was incubated with 5 ml Glutathione Sepharose 4B beads slurry (Cytiva ...
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bioRxiv - Molecular Biology 2023Quote: ... The cells were cultured to confluence and passaged 4 times before plating to 8-chamber slides (Thermo Fisher Scientific Cat#177445) coated with Matrigel (Thermo Fisher Scientific Cat# CB-40230 or Corning Cat# 356230 ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were sonicated twice for 30 s and then cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Amylose beads (Biolabs ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Cell lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
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bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
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bioRxiv - Bioengineering 2023Quote: ... It was further baked at 95°C for 8 min and developed by submerging in SU-8 developer (Microchem SU-8 developer, Fisher Scientific) for at least 15 min ...
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bioRxiv - Bioengineering 2024Quote: ... It was further baked at 95°C for 8 min and developed by submerging in SU-8 developer (Microchem SU-8 developer, Fisher Scientific) for at least 15 min ...
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bioRxiv - Physiology 2023Quote: ... Hydroxyguanosine samples were stained with 1:500 8-OHdG/8 polyclonal antibody (ThermoFisher) overnight ...
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bioRxiv - Genomics 2020Quote: ... A subsample of approximately 25 larvae was collected from each replicate jar on days 4 and 8 and stored in RNAlater (Thermo Fisher Scientific) for gene expression profiling.
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bioRxiv - Cancer Biology 2019Quote: Viability was assessed after 8 weeks of culture by staining with 4 μM ethidium homodimer-1 and 2 μM calcein AM (Thermo Fisher Scientific). Any condition where at least one viable cell was identified was considered positive for the presence of viable cells ...
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bioRxiv - Immunology 2019Quote: ... 11-7139-42 or 85BRD, cat.: 11-7136-42), IL-4-PE (8D4-8, cat.: 12-7049-42) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11 ...
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bioRxiv - Cancer Biology 2020Quote: ... A431RON (Figure 3A-B and 4) or A431RON-K1114M (Figure S3B) cells were seeded in 8-well chamber slides (Nunc Lab-Tek) at a density of 30,000/well and allowed to adhere overnight ...
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bioRxiv - Bioengineering 2022Quote: ... culture dishes were coated at 4 °C overnight with collagen I at a 1:8 concentration diluted in ddH2O (Gibco, #A10483-01). The next day ...
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bioRxiv - Neuroscience 2019Quote: ... the pH was adjusted to 7.4 using 1 M Tris pH 8 and then dialyzed overnight at 4°C in Hartmann’s using a 10 kDa dialysis membrane (Fisher Scientific, Loughborough, UK). The concentration of IgG present after dialysis was determined using a modified Lowry assay (DC protein assay ...
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bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
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bioRxiv - Immunology 2020Quote: ... PBMC were collected and washed twice (400 × g, 8 min, 4°C) with cold PBS containing 1 mM UltraPureTM EDTA (Invitrogen, ThermoFisher, Basel Switzerland). In order to remove platelets ...
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bioRxiv - Immunology 2021Quote: ... Each well contained 2-4 μg protein in buffer (25mM TRIS pH 8, 150 mM NaCl) and 5x Sypro Orange (Invitrogen, stock 5000x) in a total volume of 20 μl ...
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bioRxiv - Microbiology 2022Quote: ... HeLa cells and cortical neurons were cultured on Nunc Lab-Tek II 4 or 8-well glass chamber slides (Thermo Fisher Scientific). After exposure to autophagy inducing conditions ...
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bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... Samples containing 25 µg of protein were resolved on 8-16% Tris-Glycine or 4-12% Bis-Tris gels (Thermo Fisher Scientific) at 150V for 45 minutes ...
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bioRxiv - Genetics 2024Quote: ... gel at 75 V for 8 h at 4°C after which gels were stained for 24 h with SYBR GOLD (Invitrogen cat. S11494) diluted 1:10000 in 1X Tris-glycine (Bio-Rad cat no ...
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bioRxiv - Microbiology 2023Quote: ... Cells were separated from supernatant by centrifugation at 15,000×g for 15 minutes at 4°C (ThermoFisher Fiberlite F15s-8×50c rotor). The supernatant was collected ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were run on NuPage™ Novex™ 4-12% Bis-Tris protein gels in MES buffer or on E-PAGE 8% 48-well gels (ThermoFisher). For native running conditions ...
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bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 14500xg for 12 minutes at 4°C in a Fiberlite F21-8 x 50y Fixed-Angle Rotor (Thermo Fisher, USA). The synaptosome-rich interface between 10% and 23% Percoll layers was collected and resuspended in 30 volumes of HB ...
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bioRxiv - Immunology 2022Quote: ... membranes were washed three times with PBS-T and then incubated with appropriate secondary antibody conjugated with alkaline phosphatase that provides a visual color change upon addition of the chromogenic substrate (mixture of BCIP (5-bromo-4chloro-3-indolyl phosphate-catalog# 34040) and NBT (nitro-blue tetrazolium chloride, catalog# 34035 from Thermo Scientific)).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... while IL-8 production was evaluated using human IL-8 ELISA kit (Thermo Scientific), with assay range of 2-250 pg/mL.
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bioRxiv - Cell Biology 2020Quote: ... supplemented with 8% FBS (GIBCO) and 20% L929 supernatant and antibiotics (penicillin ...
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bioRxiv - Plant Biology 2022Quote: ... Di-8-ANEPPS (Molecular Probes, Life Technologies ...
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bioRxiv - Immunology 2022Quote: 8-well Labtek chambers (Nunc) were coated with 15 μg/ml of anti-IgM and/or anti-IgG with or without 0.5 μg/ml of mouse histidine-tagged ICAM-1 (Sino Biological ...
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bioRxiv - Genetics 2020Quote: ... pH 8 solution (Ambion, #AM9262), add 1 mL 14.3M β-mercaptoethanol (Sigma Aldrich ...
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bioRxiv - Bioengineering 2020Quote: ... Essential 8 Flex (Life Technologies), on growth factor-reduced Matrigel® (Corning)-coated plates and routinely passed with Relesr (Stem Cell Technologies) ...