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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 8 Bromo 6 chloro 2H chromene 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated using NuPAGE 3–8% Tris-Acetate Protein Gels (Life Technologies/Gibco®, Karlsruhe, Germany). For the detection of fibronectin ...
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bioRxiv - Developmental Biology 2022Quote: ... Proteins were separated using NuPAGE 3–8% Tris-Acetate Protein Gels (Life Technologies/Gibco®, Karlsruhe, Germany). For the detection of fibronectin ...
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bioRxiv - Cancer Biology 2022Quote: ... Proteins were blotted onto PVDF membranes using iBlot (program 3 for 8 min; Invitrogen, Carlsbad, CA, USA). Blots were blocked in 5% nonfat dry milk (NFDM ...
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bioRxiv - Cell Biology 2023Quote: Equal amounts of reduced protein were loaded into 3-8% Tris-Acetate Mini Gels (Thermo Fisher Scientific) and separated electrophoretically by molecular weight ...
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bioRxiv - Cancer Biology 2023Quote: ... Proteins were blotted onto PVDF membranes using iBlot (program 3 for 8 min; Invitrogen, Carlsbad, CA, USA). Blots were blocked in 5% nonfat dry milk (NFDM ...
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bioRxiv - Cell Biology 2024Quote: ... 50 μg/lane of lysates were electrophoresed on NuPAGE 3–8% Tris-Acetate Gels (Thermo Fisher Scientific), while 10 μg/lane of lysates were electrophoresed on Bolt 4–12% Bis-Tris Plus Gels (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by NuPAGE 3-8% or 4-12 % Tris-Acetate Midi Gel (Invitrogen, Cat# WG1402BX10) and transferred to nitrocellulose membranes (Fisher ...
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bioRxiv - Developmental Biology 2024Quote: ... and 8 mM Na2HPO4) including 3 μM acetoxymethyl (AM) ester calcium crimsonTM dye (Invitrogen, Carlsbad, CA, USA) for 30 min at 27 °C ...
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bioRxiv - Cancer Biology 2024Quote: ... A standardized quantity of protein was resolved using NuPAGE Tris-Acetate Protein Gel (3-8%)(Thermo Fisher) or NuPAGE Bis-Tris Protein Gel (4-12% ...
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bioRxiv - Cell Biology 2019Quote: ... with NuPAGE 3-(N-morpholino) propane sulfonic acid (MOPS) sodium dodecyl sulfate (SDS) running buffer (Invitrogen). Proteins were then transferred to polyvinylidene difluoride (PVDF ...
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bioRxiv - Microbiology 2020Quote: ... Parasitemias were determined on day 3 by staining with SYBR Green 1 nucleic acid stain (Invitrogen) at 1:2000 dilution in PBS/0.3% BSA for 20 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... C12 (4,4-Difluoro-5-Methyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen) at 2 mg/ml in PBS for 10 min or with Laurdan (see above ...
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bioRxiv - Cell Biology 2019Quote: ... Acid washed glass coverslips were incubated for 10 min with 20% 3-Aminopropyltriethoxysilane (430941000; ACROS Organics) diluted in acetone and subsequently washed with ddH2O for 15 min ...
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bioRxiv - Cell Biology 2023Quote: ... To deplete Ring1B-AID the cells were treated with indole-3-acetic acid (auxin, Life Technologies) at 500 µM whereas to deplete T7-dTAG–TAF1 the cells were treated with 20 µM 5,6-Dichloro-1-beta-D-ribofuranosylbenzimidazole for 1h ...
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bioRxiv - Cancer Biology 2019Quote: Thymus and femurs from 6-8 week old mice were harvested and subjected to red blood cell lysis (00-4333-57, Invitrogen). Thymocytes and bone marrow cells were counted by Beckman Coulter Z1 counter ...
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bioRxiv - Cancer Biology 2019Quote: ... and primary fibroblasts obtained from lungs of healthy NSG or BALB/c mice 6-8 weeks of age were cultured in Minimum Essential Medium Eagle medium with alpha modification (MEMα) (ThermoFisher) supplemented with 1x MEM Non-essential Amino Acid Solution (Sigma-Aldrich) ...
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bioRxiv - Immunology 2019Quote: ... mixtures were subjected to 8% SDS-PAGE with 6 µl of molecular weight marker (SeeBlue Plus2 Pre-Stained Standard, Invitrogen), after which proteins were transferred to nitrocellulose membranes (Amersham Hybond ECL 0.45 µm Nitrocellulose Membrane ...
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bioRxiv - Microbiology 2022Quote: Transduced BJ-hTERT cells were seeded in 6-well plates or 8-well chamber slides (Nunc™ Lab-Tek™). Cells were fixed in 4% paraformaldehyde in PBS (Alfa Aesar ...
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bioRxiv - Immunology 2021Quote: ... The supernatant was collected and ELISA was performed to detect IL-6 (8 h post stimulation, Cat # 88-7066-22, Invitrogen) and Lcn2 (24 h post stimulation ...
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bioRxiv - Neuroscience 2023Quote: ... the hippocampus form 6-8 neonatal mice was collected from both hemispheres in Hank’s buffered saline solution (HBSS, Life Technologies). Tissue was incubated in 0.25% trypsin (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... 293T cells were plated in 6 well plates at 8 x 10^5 cells / well with DMEM / 10% FBS / NEAA / Pen-Strep (Gibco). Cells growing at ∼70-80% confluency were transfected with 1 μg of the desired expression plasmid (Sigma Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... (6) Negative photoresist SU-8 2000.5 was mixed with Lissamine rhodamine B ethylenediamine (RhBen; ∼10 μg/ml; Thermo Fisher Scientific) and placed in the dark at room temperature (RT ...
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bioRxiv - Neuroscience 2021Quote: ... Nucleus staining was performed using 4’,6-diamidino-2-phenylindole (DAPI) (3 mM, D3571, Molecular Probes). Cells were counted from four randomly selected fields per culture under a confocal microscope (TCS SP8 ...
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bioRxiv - Cell Biology 2022Quote: ... 3-6 dpf larval zebrafish were anesthetized using 0.2 mg/mL Tricaine-S (Fisher Scientific, NC0872873) solution ...
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bioRxiv - Biochemistry 2021Quote: ... 2-[6-(4’-hydroxy) phenoxy-3H-xanthen-3-on-9-yl] benzoate (HPF) from Molecular Probes® and Horse radish peroxidase (HRP ...
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bioRxiv - Physiology 2021Quote: 2-3 viable human slices were incubated with Fluo4-AM (6 μM, Invitrogen cat. No. F1221) for 1h in 3 mM HEPES buffer (125 mmol/l NaCl ...
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bioRxiv - Biochemistry 2023Quote: ... batches of 3-6 SC-islets were embedded in extracellular matrix (Geltrex, Gibco, cat. n A1569601) and cultured for 7 days under perfusion ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... extended with a nucleic acid sequence encoding for 6 histidine residues (His-tag) and cloned into the mammalian expression vector pcDNA3.1(+) (Invitrogen). The soluble antigen was produced by transient gene expression in CHO cells as described previously [38] and purified from the cell culture medium by Ni-NTA resin (Roche) ...
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bioRxiv - Biophysics 2020Quote: ... were incubated for 2h together with phalloidin-FITC (Molecular Probes Inc, Eugene, OR, USA). Coverslips were mounted on microscopy slides and visualized with a HC PL APO 63x/1.40 Oil CS objective lens attached to a Leica TCS-SP5 II confocal microscope (Leica Microsystems ...
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bioRxiv - Biophysics 2023Quote: ... and stained for 2h at RT (DAPI, Thermo Fisher Scientific, D1306, 2.5 µg/ml). Washed brain slices were mounted on microscope slides in Vectashield (Vector Laboratories ...
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bioRxiv - Cancer Biology 2019Quote: Cells were labelled with 10 μM 5-bromo-2′-deoxyuridine (BrdU) (Thermo Fisher Scientific, Cat. #: B23151) for 30 min ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Molecular Biology 2020Quote: ... Proteins were resolved by SDS-PAGE using NuPAGE 3-8% Tris-Acetate precast gels (Novex, Life Technologies, Invitrogen) run for ∼1hr at 170V in 1x Tris-Acetate running buffer ...
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bioRxiv - Cell Biology 2022Quote: ... SDS-PAGE was performed using NuPAGE 4-12% Bis-Tris and 3-8% Tris-Acetate gels (Life Technologies). Nitrocellulose membranes were developed with horseradish peroxidase (HRP ...
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bioRxiv - Neuroscience 2021Quote: ... 200 µg of human serum) were separated by SDS-PAGE on 3-8% tris-acetate gels (NuPAGE, Invitrogen) under reducing conditions at 150 V (cellular fraction ...
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bioRxiv - Immunology 2020Quote: ... samples were loaded under non-reduced conditions on a precasted 3-8% Tris-Acetate SDS-PAGE gel (Invitrogen). Proteins were transferred to a nitrocellulose membrane using an iBlot2 dry blotting system (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: Up to 10μg of total protein was resolved on NuPAGE™ 3-8% Tris-Acetate Protein Gels (Invitrogen) in 1x NuPAGE™ Tris-Acetate SDS Running Buffer (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... and then analysed on Novex WedgeWell 4-12 % Tris-Glycine Gels or 3-8 % Tris-Acetate gels (Invitrogen). Tris-Glycine gels were run at 180 V for 1 hour at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were loaded onto a NuPAGE® Novex® 3–8% Tris-Acetate (Thermo Fisher, Waltham MA, USA) and run at 100V during 5h ...
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bioRxiv - Microbiology 2021Quote: ... The vesicles were formed encapsulating the fluorescent membrane-impermeable dye 8-hydroxypyrene-1,3,6-trisulfonic acid from Thermo Fisher (HPTS, 50 μM). The microfluidic platform was operated by two positive pressure-driven pump modules (MFCS-4C ...
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bioRxiv - Systems Biology 2021Quote: ... at 40°C using a linear gradient of 120 minute shallow gradient rising from 8 % (v/v) acetonitrile/0.1 % (v/v) formic acid (Fisher Scientific, UK) to 30 % (v/v ...
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bioRxiv - Cell Biology 2021Quote: ... and the pellets were dissolved in 0.1% SDS/ 0.67 M Tris-HCl pH 8 +/- 15 mM 4-acetamido-4’-maleimidylstilbene-2,2’-disulfonic acid (Thermo Fisher Scientific, USA). The samples were incubated at 37 °C for 2 h ...
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bioRxiv - Neuroscience 2021Quote: ... All data were normalized to the total protein content determined in protein pellets after neutralization with 10 N NaOH and sonication in 2% SDS plus 8 mM EDTA using a bicinchoninic acid assay (ThermoFisher Scientific) and bovine serum albumin as a standard.
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bioRxiv - Microbiology 2020Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher cat. A33952), following the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... MF002 - plus-10) and the PCR products were fractionated on 8% TBE gel stained with SYBR Gold nucleic acid stain (Invitrogen, S11494). Quantitative PCR (qPCR ...
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bioRxiv - Microbiology 2023Quote: ... N-glycans were released using peptide-N-glycosidase F (PNGase F) and labeled with 8-aminopyrene-1,3,6-trisulfonic acid (APTS) using the GlycanAssure APTS Kit (Thermo Fisher, catalog #A33952), following the manufacturer’s protocol ...
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bioRxiv - Physiology 2019Quote: ... Cardiac perfusion with DiIC18(5)-DS (1,1’-Dioctadecyl-3,3,3’,3’-Tetramethylindodicarbocyanine-5,5’-Disulfonic Acid; Thermo Fisher Scientific) diluted in 4% paraformaldehyde (PFA ...
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bioRxiv - Neuroscience 2020Quote: ... on a 4-12% bis-tris gel (Genescript) in 3-(N-morpholino)propanesulfonic acid (MOPS) buffer (Invitrogen). The gel was then fixed for 30 min in 10% acetic acid/50% methanol ...
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bioRxiv - Cell Biology 2021Quote: ... For live imaging of cells as described for fatty acids up-take we treated cells at day 5 of differentiation with BODIPY™ (10μM) C12 (4,4-Difluoro-5,7-Dimethyl-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Invitrogen). In addition ...
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bioRxiv - Immunology 2022Quote: ... resuspended in pre-sort buffer containing 3-5 nM SYTOX Green Nucleic Acid Stain (Thermo Fisher Scientific), and incubated for 20 min at 4°C ...