Labshake search
Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 8 2 4 difluorophenyl 8 oxooctanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was filtered through an 0.45 µm filter and loaded onto a pre-equilibrated 5 ml His-Trap HP column (Cytiva) ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Lysates were cleared by centrifugation at 18,000 rpm for 45 min at 4°C in a SORVAL RC6+ centrifuge with an F21S-8×50Y rotor (Thermo Scientific). The supernatant was collected and incubated with pre-equilibrated Glutathione Sepharose 4B beads (GE Healthcare ...
-
bioRxiv - Cell Biology 2023Quote: ... Frozen rat ovarian follicles were homogenized in 400 µL lysis buffer (100 mM HEPES, pH 8, 4% SDS, 1 mM EDTA, and 1× Halt protease/phosphatase inhibitor cocktail (ThermoFisher Scientific) using Zirconia/glass beads in a bead beating grinder (FastPrep24 ...
-
bioRxiv - Cell Biology 2021Quote: ... were separated using NuPAGE Bis-Tris gels (4 – 12 %) with 3-(N-morpholino)propanesulfonic acid (MOPS) or 2-(N-morpholino)ethanesulfonic acid (MES) running buffer (Thermo Fisher Scientific) followed by transferring to polyvinylidene difluoride (PVDF ...
-
bioRxiv - Microbiology 2021Quote: ... Reactions were completed in Applied Biosystems® MicroAmp® Fast 8-Tube Strips with Applied Biosystems® MicroAmp® Optical 8-Cap Strips (ThermoFisher Scientific). A sample lacking DNA template was included as a negative control with each run ...
-
bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
-
bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
-
bioRxiv - Neuroscience 2021Quote: ... seeded at 1.5 x 105 neurons per coverslip in Neuron Medium (8 % FBS, 2 % B-27, [Gibco, 17504-044], Penicillin [200 U/mL]/Streptomycin [200 μg/mL] [Gibco, 15140-130] ...
-
bioRxiv - Cell Biology 2020Quote: ... the GFP-B16 tumor spheroids were transferred to Lab-Tek II cover-glass-bottomed 8-chamber slides and imaged in HBSS supplemented with 2% FBS (all from Invitrogen) using a 63×/1.4 NA oil-immersion objective.
-
bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
-
bioRxiv - Cell Biology 2020Quote: ... pALN25: GFP-BUB1-T609A) and selected with 2 mg/ml (HeLa) or 8 mg/ml (K-562) Blasticidin (Life Technologies) (Cheeseman and Desai ...
-
bioRxiv - Cell Biology 2021Quote: ... Beads were captured on the magnet and the supernatant now containing the dissociated cells was transferred to a 15 ml Falcon tube containing 10 ml ice cold FACS buffer (DMEM w/o phenol red + 1% P/S, 2% FBS, 1 mM EDTA pH 8, all from Gibco) and centrifuged for 5 minutes at 300 g and 4°C ...
-
bioRxiv - Molecular Biology 2023Quote: Proliferating C2C12 myoblasts were transduced with lentivirus in the presence of 8 µg/ml polybrene and selected with 2 µg/ml puromycin (Invitrogen).
-
bioRxiv - Cancer Biology 2023Quote: ... 90 µL of supernatant was mixed with 410 µL of IP Dilution Buffer and antibodies (Positive antibody: 10 µL; Negative antibody: 2 µL; FoxO3a antibody: 8 µL (Cat. #720128, Invitrogen)) and incubated overnight at 4°C ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were cultivated in feeder-free medium using Essential 8 (E8) medium with 2% E8 supplement (50x) (Thermo Fisher Scientific), and 1% Penicillin Streptomycin (5000U/ml ...
-
bioRxiv - Developmental Biology 2023Quote: ... 2 and 8 dpf samples were then incubated for 20 and 80 minutes in 10 µg/ml Proteinase K (Thermofisher), respectively ...
-
bioRxiv - Synthetic Biology 2023Quote: ... the dehydrated DNA was reconstituted in phosphate-buffered saline (PBS, 137 mM NaCl, 2.7 mM KCl, 8 mM Na2HPO4, 2 mM KH2PO4, pH 7.4, Invitro-gen, Thermo Fisher Scientific). To remove any large particulate contaminants the solutions of non-functionalised oligonucleotides were syringe-filtered with polyethersulfone filters (0.22 µm pore size ...
-
A Drug-Free Pathogen Capture and Neutralizing Nasal Spray to Prevent Emerging Respiratory InfectionsbioRxiv - Bioengineering 2023Quote: ... alcohol solution or PBS was mixed with 8×104 PFU of SARS-CoV-2 mNG in 50 uL of infection media (OptiMEM (Gibco) containing 3 μg/mL TPCK-trypsin) ...
-
bioRxiv - Immunology 2023Quote: ... hTCEpi cells were stimulated by KGMTM-2 containing 20% (v/v) sterile PAO1 supernatant for 8 hours before lysis by TRI reagent (Ambion). Total RNAs were purified using Direct-Zol RNA miniprep kit (Zymo Research ...
-
Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...
-
bioRxiv - Neuroscience 2024Quote: ... and submerged in cutting buffer (8 mM Na2HPO4.2H2O, 2 mM KH2PO4, pH = 7.4) in the Micron HV650 (Thermo Fisher Scientific). Coronal sections of 60µm were cut using a frequency of 70Hz ...
-
bioRxiv - Microbiology 2019Quote: ... Coverslips were stained with 300 nM 4’,6-diamidino-2-phenylindole nucleic acid stain (DAPI; Invitrogen) in PBS for 5 min followed by washing three times in PBS at room temperature ...
-
bioRxiv - Cell Biology 2022Quote: ... 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher Scientific, Cat. no. 15630-080,) and 1% penicillinstreptomycin (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2022Quote: ... 0.1 M 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer (pH = 8.5) (ThermoFisher Scientific, Waltham, MA), 4-benzoylbenzyl-trimethylammonium chloride (PLPP ...
-
bioRxiv - Genomics 2020Quote: ... A subsample of approximately 25 larvae was collected from each replicate jar on days 4 and 8 and stored in RNAlater (Thermo Fisher Scientific) for gene expression profiling.
-
bioRxiv - Immunology 2019Quote: ... 11-7139-42 or 85BRD, cat.: 11-7136-42), IL-4-PE (8D4-8, cat.: 12-7049-42) (all from Thermo Fisher Scientific); TNFa-Pacific Blue (MAb11 ...
-
bioRxiv - Cancer Biology 2020Quote: ... A431RON (Figure 3A-B and 4) or A431RON-K1114M (Figure S3B) cells were seeded in 8-well chamber slides (Nunc Lab-Tek) at a density of 30,000/well and allowed to adhere overnight ...
-
bioRxiv - Bioengineering 2022Quote: ... culture dishes were coated at 4 °C overnight with collagen I at a 1:8 concentration diluted in ddH2O (Gibco, #A10483-01). The next day ...
-
bioRxiv - Neuroscience 2019Quote: ... the pH was adjusted to 7.4 using 1 M Tris pH 8 and then dialyzed overnight at 4°C in Hartmann’s using a 10 kDa dialysis membrane (Fisher Scientific, Loughborough, UK). The concentration of IgG present after dialysis was determined using a modified Lowry assay (DC protein assay ...
-
bioRxiv - Cell Biology 2020Quote: ... Equal masses of lysates were separated by SDS-PAGE using either 4-12% Bis Tris gels or 3-8% Tris acetate gels (Thermo Fisher Scientific). All IRS2 immunoblots were separated on 3-8% Tris acetate gels with the exception of those shown in Figures 5A and S4B ...
-
bioRxiv - Immunology 2020Quote: ... PBMC were collected and washed twice (400 × g, 8 min, 4°C) with cold PBS containing 1 mM UltraPureTM EDTA (Invitrogen, ThermoFisher, Basel Switzerland). In order to remove platelets ...
-
bioRxiv - Microbiology 2022Quote: ... HeLa cells and cortical neurons were cultured on Nunc Lab-Tek II 4 or 8-well glass chamber slides (Thermo Fisher Scientific). After exposure to autophagy inducing conditions ...
-
bioRxiv - Bioengineering 2023Quote: CHO-suspension cells were plated at a concentration of 7.5x10^4 cells/mL in Freestyle CHO Expression medium supplemented with 8 mM L-glutamine (Thermo Fisher Scientific, MA) in 125-mL disposable polycarbonate Erlenmeyer flasks with vented caps (Fisher Scientific ...
-
bioRxiv - Molecular Biology 2023Quote: ... Samples containing 25 µg of protein were resolved on 8-16% Tris-Glycine or 4-12% Bis-Tris gels (Thermo Fisher Scientific) at 150V for 45 minutes ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were run on NuPage™ Novex™ 4-12% Bis-Tris protein gels in MES buffer or on E-PAGE 8% 48-well gels (ThermoFisher). For native running conditions ...
-
bioRxiv - Microbiology 2023Quote: ... Cells were separated from supernatant by centrifugation at 15,000×g for 15 minutes at 4°C (ThermoFisher Fiberlite F15s-8×50c rotor). The supernatant was collected ...
-
bioRxiv - Genetics 2024Quote: ... gel at 75 V for 8 h at 4°C after which gels were stained for 24 h with SYBR GOLD (Invitrogen cat. S11494) diluted 1:10000 in 1X Tris-glycine (Bio-Rad cat no ...
-
bioRxiv - Biochemistry 2023Quote: ... and centrifuged at 14500xg for 12 minutes at 4°C in a Fiberlite F21-8 x 50y Fixed-Angle Rotor (Thermo Fisher, USA). The synaptosome-rich interface between 10% and 23% Percoll layers was collected and resuspended in 30 volumes of HB ...
-
bioRxiv - Cell Biology 2020Quote: 8%-16% precast Novex Tris-Glycine gels (ThermoFisher Scientific,Waltham, MA) were used following standard protocols established in our laboratory [20].
-
bioRxiv - Molecular Biology 2020Quote: ... resolved on a 3-8% Tris-Acetate SDS PAGE gel (Invitrogen), and transferred onto a PVDF membrane ...
-
bioRxiv - Cell Biology 2020Quote: Cells were seeded on a LabTek 8-well chamber (Thermo Scientific) coated with poly-D-lysine hydrobromide (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2020Quote: ... for 8 min at 180V in 1x MES buffer (Thermo Scientific). Proteins were fixated and stained with a Coomassie solution (0.25% Coomassie Blue G-250 [Biozym] ...
-
bioRxiv - Developmental Biology 2021Quote: ... 5% CO2 in Essential 8 medium (Cat. # A1517001, Thermo Fisher Scientific) and passaged every 3 – 4 days using Versene (Cat ...
-
bioRxiv - Developmental Biology 2020Quote: ... Amplicons were subcloned into pCR™8/GW/TOPO® (Invitrogen) to create entry vectors for subsequent cloning into the Tol2-GFP-destination vector pT2KHGpzGATA2C1 as described by 27 ...
-
bioRxiv - Developmental Biology 2020Quote: ... the NuPAGE™ 3 to 8% Tris-Acetate gels (Invitrogen, EA03755) were used with Tris-Acetate SDS Running Buffer (pH 8.24 ...
-
bioRxiv - Plant Biology 2022Quote: ... Di-8-ANEPPS (Molecular Probes, Life Technologies, cat. # D3167, Oregon, USA): a 10 mM stock solution in DMSO was stored in 10 μL aliquots at -20°C.
-
bioRxiv - Cell Biology 2022Quote: Cells were seeded in CC2-coated 8-chamber slides (ThermoFisher, #154941) two days prior to experimentation and transfected as indicated ...
-
bioRxiv - Biochemistry 2022Quote: ... Destination vectors include pDest-8 (native baculovirus expression, Thermo Fisher Scientific), pDest-636 (His6-MBP fusion for baculovirus expression ...