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Citations for Thermo Fisher :
201 - 250 of 286 citations for 7H Dodecafluoroheptanoyl chloride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... Red cell lysis was performed in 10 ml ACK (Ammonium-Chloride-Potassium) lysing buffer (Invitrogen, Cat # 11814389001) following manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Erythrocytes were lysed for 3 min using an ammonium chloride-potassium phosphate buffer (ACK Lysing Buffer, Gibco). Cell numbers were counted in Turk’s solution using a Neubauer chamber.
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bioRxiv - Biophysics 2022Quote: ... The conductivities of each alkali chloride were recorded using an Accumet AB200 pH/Conductivity Benchtop Meter (Fisher Scientific). The results were as follows ...
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bioRxiv - Bioengineering 2022Quote: ... filtered trypan blue solution (0.4% trypan blue in 0.81% sodium chloride and 0.06% dibasic potassium phosphate; Life Technologies) was prepared and incubated for 2 min at room temperature for viable/non-viable cell imaging under the phase contrast microscope ...
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bioRxiv - Biochemistry 2019Quote: ... was incubated overnight in 0.1 M sodium formate / 0.15M sodium chloride/ 0.1% reduced Triton X-100 pH 3.5 with dialysis against the same buffer (20kDa MWCO, ThermoFisher) to convert TPP1 to a mature/active form and to remove the cleaved pro-domain ...
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bioRxiv - Bioengineering 2020Quote: ... EVs are either non-specifically labeled using the fluorescent membrane dye Octadecyl Rhodamine B Chloride R18 (Life Technologies, catalog# O246 ...
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bioRxiv - Microbiology 2020Quote: ... diluted in Earle’s balanced salt solution supplemented with 200 mg/ml Calcium Chloride (Gibco, Life Technologies, Renfrew, UK), at 37°C with continual gentle mixing of the tube ...
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bioRxiv - Microbiology 2020Quote: ... diluted in Earle’s balanced salt solution supplemented with 200 mg/ml Calcium Chloride (Gibco, Life Technologies, Renfrew, UK), at 37°C with continual gentle mixing of the tube ...
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bioRxiv - Microbiology 2020Quote: ... in ice-cold RIPA buffer (150 mM sodium chloride, 1.0% Triton X-100, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0; Thermo Fisher Scientific), supplemented with complete Protease Inhibitor Cocktail (Sigma Aldrich ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once with Krebs-Ringer’s buffer containing 1.8 mM calcium chloride and loaded with Fluo-4 AM (1 μg/μl; ThermoFisher) plus 10 μl 0.2 % pluronic acid for 30 min at 37 °C ...
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bioRxiv - Physiology 2023Quote: ... The pellets were then resuspended and incubated with ammonium-chloride-potassium (ACK) lysing buffer (Thermo Fisher Scientific, USA) for 3 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... For nuclear staining 0.5µg/µL of DAPI (4’, 6-diamidino-2-phenylindole, dihydro-chloride, Invitrogen. Cat. No.: D3571) was added along with secondary antibody ...
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bioRxiv - Microbiology 2024Quote: ... paramagnetic beads were washed three times with 1X wash buffer (50 mM Tris-HCl pH 7.5 – 150 mM sodium chloride – 0.05% Tween-20 – 1 mM dithiothreitol) using DynaMag-2 magnetic stand (Invitrogen). Mpro-Avi (6 ug ...
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bioRxiv - Bioengineering 2021Quote: ... The virus was then dialyzed with 1× phosphate buffered saline (pH 7.2) supplemented with 50 mM sodium chloride and 0.0001% Pluronic F68 (Thermo Fisher) using 50 kDA filters (Millipore) ...
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bioRxiv - Microbiology 2021Quote: ... was accomplished by reacting the respective oligosaccharide glycamines with Lissamine rhodamine B sulfonyl chloride (LRSC) (Acros Organics, Belgium, USA) as described previously (64) ...
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bioRxiv - Developmental Biology 2020Quote: ... followed by Poly(A) tailing procedure and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). pCMV-ancBE4max (pCMV_AncBE4max was a gift from David Liu (Addgene plasmid # 112094 ...
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bioRxiv - Microbiology 2020Quote: Approximately 5 μg of BAC DNA was linearized with NotI and full-length RNA was obtained by in vitro transcription with T7 RNA polymerase followed by lithium chloride precipitation according to the manufacturer’s protocol (mMessage-mMachine T7 Kit; Ambion). 5 μg of RNA was electroporated into 5×106 BHK-21 cells using the Amaxa nucleofector 2b (program A-031 ...
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bioRxiv - Immunology 2022Quote: ... for 5 minutes and pellets were resuspended in Ammonium-Chloride-Potassium (ACK) lysing buffer (Thermo Fisher Scientific, Catalog # A1049201) to lyse RBC (erythrocytes removal performed at R.T ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were fixed and stained with 200uL of 1 mg/mL solution of Nitro Blue Tetrazolium Chloride (Invitrogen, N6495). Six biological replicates were performed ...
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bioRxiv - Bioengineering 2023Quote: ... HA-tetrazine was synthesized as described previously using 79 kDa HA with molar equivalents of 1:1:0.25 of HA-repeat units to 4-(4,6-dimethoxy-1,3,5-triazin-2-yl)-4-methylmorpholinium chloride (DMTMM) (Thermo Fisher Scientific) to tetrazine-amine (Chem-Impex).[16] 1H NMR shifts of pendant tetrazine groups at δ8.5 (2H ...
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bioRxiv - Neuroscience 2024Quote: ... Virus was diluted in sterile 0.9% w/v sodium chloride in low protein binding collection tubes (Life Technologies Ltd) and stored at 4°C ...
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bioRxiv - Developmental Biology 2024Quote: ... Samples were washed gradually into 2X SSC buffer (0.3 M sodium chloride, 30 mM sodium citrate or AM9763, Invitrogen), and then gradually from SSC to PBT ...
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bioRxiv - Microbiology 2023Quote: ... Red blood cells (RBCs) were then lysed by resuspending cells in ammonium-chloride-potassium (ACK) lysis buffer (Thermo Fisher) for 2 min ...
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bioRxiv - Microbiology 2020Quote: ... Sections were then incubated in a 48 °C prewarmed saline-sodium citrate wash buffer (30 mM sodium citrate, 300 mM sodium chloride, pH7.4, Invitrogen, France) for 20 minutes ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthesized by in vitro transcription with 1μl of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE T7 Ultra kit #AM1345, Ambion). the pCS2+_ancBE4max-SpymacCas9 has been linearized using KpnI restriction enzyme ...
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bioRxiv - Immunology 2021Quote: ... Spleens and lungs were mashed through a 70-μm strainer before red blood cells were lysed using ammonium-chloride-potassium (ACK) lysis (Gibco). Cells were washed with PBS containing 1% FCS and surface receptors were stained using various antibodies ...
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bioRxiv - Neuroscience 2020Quote: ... red blood cells (RBCs) were lysed for 5 min at room temperature using ammonium-chloride-potassium (ACK) buffer (Life Technologies). After centrifugation ...
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bioRxiv - Genomics 2022Quote: ... The undigested pieces of lamina propria were then washed thoroughly with PBS (with calcium and magnesium chloride; Gibco 14080-055) to get rid of all EDTA ...
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bioRxiv - Synthetic Biology 2019Quote: ... of DNA was prepared with specific combinations of plasmids and brought up to a volume of 100 µL using 0.15 M sodium chloride (NaCl, Fisher Scientific). Next ...
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bioRxiv - Developmental Biology 2019Quote: ... In vitro transcription of Tol2 mRNA and lithium chloride precipitation/purification was performed using the SP6 mMessenger Kit (Ambion #AM1340) according to manufacturers guidelines.
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bioRxiv - Plant Biology 2021Quote: ... protoplasts were resuspended in lysis buffer (45mM magnesium chloride, 30mM sodium citrate, 20mM MOPS, 0.5% triton, 2% 4’,6-diamidino-2-phenylindole (DAPI, Thermo Scientific), pH 7 ...
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bioRxiv - Developmental Biology 2020Quote: ... mRNAs were synthetized by in vitro transcription with 1μl of GTP added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Immunology 2021Quote: ... Red blood cells were then lysed by adding 3 ml of ammonium-chloride-potassium (ACK) lysis buffer (Invitrogen, Carlsbad, CA) for 4 min at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Red blood cells were lysed with 1 ml ammonium-chloride-potassium (ACK) lysis buffer (Quality Biological, Gaithersburg, MD, USA) for 1 minute and neutralized with ice-cold DMEM (Gibco). Cells were processed for RNAseq in FACS buffer (2% fetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Each plasmid was linearized with NotI restriction enzyme and mRNAs were synthesized by in vitro transcription with 1 µL of GTP from the kit added to the mix and lithium chloride precipitation (using the mMESSAGE mMACHINE sp6 Ultra kit #AM1340, Ambion).
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bioRxiv - Biophysics 2022Quote: ... 0.020 M potassium phosphate monobasic), sodium chloride (BioXtra grade, purity ≥99.5%) and D-(-)-Fructose (HPLC grade, purity ≥99%) from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2023Quote: ... 10 μM gramicidin was added to the internal recording solution to induce chloride-impermeable pore formation along with 25 μM Alexa Fluor 568 hydrazide Na+ salt (Invitrogen) to visualize any potential pore rupture ...
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bioRxiv - Immunology 2022Quote: ... and red blood cells of fresh spleens or bone marrow were lysed in ammonium-chloride-potassium buffer lysing buffer (Gibco) for 3 min ...
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bioRxiv - Cell Biology 2023Quote: ... cells were resuspended in growth media containing octadecyl rhodamine B chloride (R18, ThermoFisher, 1:1000 dilution of 10mg/ml stock), centrifuged ...
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bioRxiv - Immunology 2023Quote: ... Cells were centrifuged at 1500 rpm for five minutes and then resuspended in 5mL of Ammonium-Chloride-Potassium (ACK) lysis buffer (Gibco) on ice for 10 minutes ...
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bioRxiv - Microbiology 2024Quote: ... red blood cells were lysed by addition of 1 ml ammonium chloride-potassium carbonate (ACK) lysis buffer (Life Technologies, A1049201). ACK was neutralized by the addition of cRPMI ...
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bioRxiv - Cancer Biology 2024Quote: ... The pellet was resuspended in 200–500 µl of ACK (ammonium-chloride-potassium) RBC lysis buffer (Thermo Fisher Scientific, A1049201) and incubated for 1 min on ice ...
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bioRxiv - Molecular Biology 2020Quote: Single- and double-stranded oligonucleotide substrates were diluted in TBST with 0.1% BSA and 3 mM magnesium chloride to 1 μM final concentrations Previously mentioned enzymes and RNase A (EN0531, Thermo Fisher) were used at a 1:200 final dilution and incubated with indicated nucleic acid substrates for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2020Quote: ... Enzymatic treatments were done in staining buffer supplemented with 3 mM magnesium chloride with 1:200 dilutions of RNase T1 (EN0541, Thermo Fisher), RNase III (ShortCut RNase III ...
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bioRxiv - Cell Biology 2020Quote: ... solutions of E3 + Tricaine and E3 + Tricaine + 135 mM sodium chloride were prepared and supplemented with 10µM of CoroNa Green (Invitrogen Cat#C36675). A wildtype larva was mounted in the device as described above and confocal image stacks were collected over time as the two CoroNa Green containing media were flowed into either chamber of the device ...
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bioRxiv - Microbiology 2020Quote: ... BMDM with bacteria were incubated for 30 min at 37°C/5% CO2 and then washed three times with warm DPBS++ (+Calcium and +Magnesium Chloride – Gibco 14040). One mL fresh DMEM-10 with Gentamicin (50 μg/mL ...
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bioRxiv - Neuroscience 2022Quote: ... The spleen was mashed with the plunger of a syringe and treated with ammonium-chloride-potassium (ACK) lysis buffer (Gibco™) to remove erythrocytes ...
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bioRxiv - Immunology 2022Quote: ... membranes were washed three times with PBS-T and then incubated with appropriate secondary antibody conjugated with alkaline phosphatase that provides a visual color change upon addition of the chromogenic substrate (mixture of BCIP (5-bromo-4chloro-3-indolyl phosphate-catalog# 34040) and NBT (nitro-blue tetrazolium chloride, catalog# 34035 from Thermo Scientific)).
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bioRxiv - Neuroscience 2022Quote: ... 1xPBS was replaced with direct stochastic optical reconstruction microscopy (dSTORM) photo-switching buffer consisting of 50 mM Tris pH 8 solution supplemented with 10 mM sodium chloride (ThermoFisher Scientific), 10% glucose (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... or dequalinium chloride (ranging 1 to 500 μg/ml) in Flat white 96-well plates with optical bottom (Thermo Scientific Nunc), at a final OD600nm of 0.01 ...