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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 7H Diimidazo 1 5 a 1 5 4 de quinoxaline 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... which included 5 µL 4× Taqman Fast Advanced Master Mix (Thermo Fisher Scientific), 0.4 µL of each primer (tat 2.0 and rev ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were loaded with 5 μM of Fluo-4-AM (Thermo Fisher Scientific) for 50 min at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 for 4 minutes and resuspended in E8 medium (Thermo Fisher Scientific) and 10 μM Y-27632 Rho-kinase inhibitor (ROCKi ...
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bioRxiv - Bioengineering 2023Quote: ... calcium imaging was performed using 5 μM Fluo-4-AM (ThermoFisher, F14201, US) in Krebs-Ringer’s solution containing NaCl 119 mM ...
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bioRxiv - Genomics 2023Quote: ... hiPSC-CMs were loaded with Fluo-4-acetoxymethyl (AM)-ester (5 μM, Invitrogen) in Tyrode’s buffer (135 mM NaCl ...
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bioRxiv - Molecular Biology 2023Quote: ... 3 × 10-4 M MTG and 5% Protein-Free Hybridoma Media II (Gibco).
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bioRxiv - Biochemistry 2024Quote: ... permeabilized with 0.2% Triton X-100 in PBS for 5 mins at room temperature and incubated in primary ZO-1 antibody (ZO-1 polyclonal rabbit antibody, Invitrogen #61-7300, 1:2,000) diluted into blocking buffer for 2 hrs at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... 1 μg of this plasmid was transfected into 5×105 SH-SY5Y cells with Lipofectamine LTX PLUS (1:1 ratio; Invitrogen) and selected for colonies with G418 (0.5 mg/ml) ...
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bioRxiv - Developmental Biology 2020Quote: ... and cultured at 37 °C in a humidified 5% CO2 atmosphere with kidney culture medium (F12:DMEM (1:1) + Glutamax (Gibco) supplemented with 10% fetal bovine serum and penicillin-streptomycin)(Ihermann-Hella & Kuure ...
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bioRxiv - Systems Biology 2019Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem) ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were passaged at a density of 1:2 to 1:8 after reaching ~80% confluency by detaching with 5 mM EDTA in PBS (Invitrogen 15575-038 diluted in sterile 1X PBS ...
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bioRxiv - Cancer Biology 2021Quote: ... Slides were blocked in 1% BSA and then stained with indicated antibodies against Claudin-5 (Invitrogen 35-2500 1:50), Ad5 (Abcam ab6982 1:100) ...
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bioRxiv - Neuroscience 2020Quote: ... with 1% PBS-T (1% Triton X-100 in 1x PBS) and blocked in 1% PBS-T containing 5% normal goat serum (ThermoFisher) for one hour ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Microbiology 2019Quote: ... at a 1:10000 dilution in 5% skimmed milk-PBST and 1:20000 secondary HRP Rabbit-anti-mouse antibody (Invitrogen) in 5% skimmed milk-PBST16.
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bioRxiv - Genomics 2019Quote: ... Cells were resuspended in 1 ml IP lysis buffer [25 mM Tris-HCl pH 7.5 / 150 mM NaCl / 1% NP-40 / 1 mM EDTA / 5% glycerol / Halt protease inhibitor cocktail (Thermo scientific) / Halt phosphatase inhibitor cocktail (Thermo scientific)] and incubated on ice for 1 h ...
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bioRxiv - Immunology 2021Quote: ... 1.75x103 cells were loaded into individual well of a 96 well v-bottom plate in RPMI/5% (v/v) FBS/2mM EDTA/1% (v/v) Penicillin/Streptomycin/1% (v/v) L-Glutamine (ThermoFisher)/0.1% (v/v ...
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bioRxiv - Developmental Biology 2020Quote: ... 25 mM Tris-HCl pH 7.4, 150 mM NaCl, 1 mM EDTA, 1% NP-40 and 5% glycerol; Thermo Fisher) containing 1x protease inhibitor cocktail III (Calbiochem ...
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bioRxiv - Cell Biology 2020Quote: ... 0.1 M MgCl2, 0.1M Tris HCL, 1% Tx-100, 1x sBlock, 1% sodium deoxycholate, 5 units/mL DNAse I – Thermo Scientific #89836 ...
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bioRxiv - Systems Biology 2021Quote: ... Native protein complexes from platelets were obtained by incubating the washed pallet with Pierce IP lysis buffer (25 mM Tris•HCl pH 7.4, 150 mM NaCl, 1% NP-40, 1 mM EDTA, 5% glycerol) (Thermo Scientific) containing protease and phosphatase inhibitors at 4 °C for 5 minutes ...
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bioRxiv - Pathology 2021Quote: ... pellets were resuspended in 50μl of 5% SDS containing phosphate buffered saline and cytoplasmic fractions were mixed 1:1 with 10% SDS solution (Thermofisher) to give a final 5% SDS concentration ...
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bioRxiv - Microbiology 2020Quote: ... 1 μL (10μM) reverse primer (ldh4_R, 5’-AATCACAGCAGCCCCTTG-3’) and 1 μL (1 U/μL) Platinum Taq DNA polymerase (Invitrogen, 10966-018) in a 50 μL total reaction volume ...
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bioRxiv - Neuroscience 2023Quote: ... and incubated in culture media (66% BME, 25% Hanks, 5% FBS, 1% N-2, 1% penicillin, streptomycin and glutamine; all from Invitrogen) and 0.66% D-(+)-Glucose (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... SK-N-BE(2)-C were grown at 37 °C and 5% CO2 in a media containing 1:1 mixture of EMEM (ATCC) and F12 medium (Gibco) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... were coated with 100 µL of recombinant SARS-CoV-2 S protein (Wuhan-1 strain and BA.5) at a concentration of 1 µg/mL in PBS (Gibco) at 4°C overnight ...
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bioRxiv - Physiology 2023Quote: ... 0.5 % non-fat dry milk the membrane was incubated overnight with rabbit polyclonal anti-calstabin 1 (PA 1-026, ThermoFisher) (1:1200 in blocking buffer) ...
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bioRxiv - Physiology 2023Quote: ... with a 1:5 – 1:10 passaging rate onto cultureware coated with Geltrex (LDEV-Free, hESC-Qualified; Gibco, Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and resuspended with 5 mg/mL collagenase and 1 mg/mL DNase I in 1 mL of RPMI 1640 without phenol red (Gibco). After incubation at 37°C for a half hour ...
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bioRxiv - Developmental Biology 2024Quote: ... The following primary antibodies were used: Zn-5 (1/200; ZIRC, University of Oregon) and GFP (1/1000; Molecular Probes). After several washes in PBT1% ...
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bioRxiv - Developmental Biology 2024Quote: ... and cultured in SCs culture medium (growth medium: 20% FBS, 5% horse serum, 1% penicillin/streptomycin, 1% GlutaMAX supplement (Gibco) and 2.5 ng/ml FGF (R&D systems ...
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bioRxiv - Synthetic Biology 2023Quote: ... and split 1:5 or 1:10 into a new tissue culture (TC)–treated T75 flask (Thermo Scientific ref 156499).
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bioRxiv - Developmental Biology 2023Quote: ... coated plates in growth media (DMEM with 20% FBS, 5% horse serum, 1% pen-strep, 1% glutamax (above from Gibco), 0.1% chick embryo extract (MPbio ...
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bioRxiv - Cell Biology 2023Quote: ... The MCF10A human mammary epithelial cell line was cultured in DMEM/F12 (1:1) media supplemented with 5% (v/v) horse serum (Gibco), 20 ng/ml epidermal growth factor (Peprotech) ...
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bioRxiv - Microbiology 2024Quote: ... Tenfold dilutions were used to infect confluent Vero E6 cells in a 96-well plate in MEM (5% FBS, 1% penicillin-streptomycin, 1% kanamycin, 3% amphotericin B (Gibco)) at 37°C and 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... membrane pellets were gently resuspended in 6 ml of resuspension buffer (25% [wt/wt] sucrose, 5 mM Tris, 30 mM MgCl2, 1 tablet of Pierce Protease Inhibitor Mini Tablets, EDTA-Free [Thermo Scientific], 5 U Benzonase [Novagen]), suspensions were incubated with gentle mixing for 30 min at room temperature to degrade DNA ...
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bioRxiv - Cell Biology 2022Quote: ... Itgb1 (forward 5’-ATGCCAAATCTTGCGGAG AAT, reverse 5’-TTTGCTGCGATTGGTGACATT), and GAPDH (forward 5’– GGTCATCCATGACAACTT, reverse 5’–GGGGCCATCCACAGTCTT) are designed and synthesized by Invitrogen (Shanghai, China).
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bioRxiv - Plant Biology 2020Quote: ... Separation was obtained on a Reverse-Phase Acclaim Polar Advantage II column (C18, 4.6 × 250 mm length, 5 μm particle size) (Thermo Scientific, Wilmington, DE, USA) at 37°C with a gradient elution programme of 1 ml min-1 flow rate with an increasing ACN/acetic acid gradient ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10−5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Bioengineering 2021Quote: ... 5 × 10-5 M 2-mercaptoethanol (Gibco, 31350-010), 1X Minimal Essential Medium (MEM ...
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bioRxiv - Bioengineering 2023Quote: ... stained with 5 ng/mL dichlorotriazinylaminofluorescein (5-DTAF, Invitrogen), and mounted in custom grips at a 10 mm gauge length ...
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bioRxiv - Developmental Biology 2020Quote: The retinas of juveniles (n = 6 retinas from 5 animals) and adults (n = 5 retinas from 4 animals) were dissected out and put in RNAlater (Ambion Inc., Austin, TX, USA). RNA extraction was performed using the RNeasy mini kit (Qiagen ...
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bioRxiv - Cancer Biology 2023Quote: ... ITS (5 μg/ml insulin, 5 μg/ml transferrin, and 5 ng/ml sodium selenite, Gibco), and antibiotics (100 U/ml penicillin and 0.1 mg/ml streptomycin) ...
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bioRxiv - Biochemistry 2021Quote: ... or with 0.5 µg/ml doxycycline for 2-3 days) were combined in a 1:1 ratio and loaded with 5 µg/ml Indo-1 (Molecular Probes, AAT Bioquest, Sunnyvale, USA) and 0.04% of pluronic F-127 (AAT Bioquest ...
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bioRxiv - Neuroscience 2024Quote: ... on day 6 and split 1:5 with a chemically defined neural medium including DMEM/F12 supplemented with GlutaMAX (1/1; Thermo-Fisher Scientific; Cat. No. 35050-061), Neurobasal medium (1/1 ...
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bioRxiv - Biophysics 2021Quote: ... buffer B (5 mM Tris-HCl pH 8.0, 10 mM MgCl2, 1 mM EDTA; Ambion), BSA-biotin (Sigma-Aldrich ...
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bioRxiv - Biophysics 2020Quote: ... Adherent cells were passaged at a 1:5 dilution using 0.05% trypsin EDTA (Gibco #25200056). PLB cells were a kind gift from Dr ...
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bioRxiv - Cell Biology 2020Quote: ... N2B27 1-5% KSR base media was supplemented with 1x NEAA (ThermoFisher Scientific 11140-050); 10 ng/mL recombinant human LIF (hLIF ...
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bioRxiv - Cell Biology 2020Quote: ... cells were incubated in 1 mL solution containing 5 μL Vybrant DiO (Molecular Probes, V22886) or Vybrant DiD (Molecular Probes ...
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bioRxiv - Developmental Biology 2021Quote: ... containing 5% FBS and 1% MEM non-essential amino acids (NEAA) solution (Thermo Fisher Scientific) in a 96-well clear flat bottom microplate (Corning) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 5 min incubation (at 37 °C) of Hoechst dye (Life Technologies; 1:15,000 dilution) in warm PBS ...