Labshake search
Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 7 nitro 9H fluorene 2 sulfonic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2020Quote: ... Cell death was assessed by 7-aminoactinomycin D (7-AAD, Affymetrix eBioscience, San Diego, CA) staining and flow-cytometry (FACSCantoII™ ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol)ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
-
bioRxiv - Biochemistry 2020Quote: ... The proteins were incubated with 20-fold molar excess of either IANBD [N,N ′ -dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol) ethylenediamine or Alexa Fluor 546 (AF546) maleimide (Molecular Probes] for 2 h at RT ...
-
bioRxiv - Microbiology 2020Quote: ... HEK 293T/17 (ATCC® CRL-11268) and LLCMK-2 (monkey kidney epithelial cells, ATTC CCL-7) were maintained in Tissue Culture Medium (DMEM (Invitrogen) supplemented with 10% Fetal Bovine Serum (Sigma) ...
-
bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
-
bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
-
bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
-
bioRxiv - Biophysics 2019Quote: ... N-((2-(iodoacetoxy)ethyl)-N-Methyl)- amino-7-Nitrobenz-2-Oxa-1,3-Diazole (IANBD ester) and Oregon Green 488 maleimide were purchased from LIFE TECHNOLOGIES LTD (Paisley ...
-
bioRxiv - Biophysics 2019Quote: ... N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)ethylenediamine] (Thermo Fisher Scientific, Inc., Waltham, MA). Y-ATRAM (Table 1 ...
-
bioRxiv - Biochemistry 2021Quote: ... with a 10-fold excess of N,N’-dimethyl-N-(iodoacetyl)-N’-(7-nitrobenz-2-oxa-1,3-diazol-4-yl) ethylenediamine (IANBD-amide, Molecular Probes). After 90 min on ice ...
-
bioRxiv - Immunology 2021Quote: ... and then transferred to nitrocellulose membrane for 7 min at 20 V using the iBlot 2 Dry Blotting System (Invitrogen). Where protein phosphorylation status was investigated ...
-
bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
-
bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
-
bioRxiv - Plant Biology 2023Quote: Quantification of ROS in guard cells of epidermal peels was measured using 20 µM 2’,7’-dichlorodifluorescein diacetate (H2DCFDA; Invitrogen) according to (30) ...
-
bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
-
bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...
-
bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
-
bioRxiv - Microbiology 2023Quote: ... 7% of heat-inactivated fetal bovine serum (FBS) and 2% of G-418 disulfate 50mg/mL (all from ThermoFisher Scientific).
-
bioRxiv - Molecular Biology 2023Quote: The staining of oxidants was performed with the commercially available 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (#C6827, Thermo Fisher Scientific, USA). Cryo-sections (10 µm ...
-
bioRxiv - Bioengineering 2024Quote: COS-7 cells (ATCC, CRL-1651) and HeLa cells (ATCC, CCL-2) were cultured in high-glucose DMEM (Gibco, 21063029) supplemented with 10% fetal bovine serum (FBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
-
bioRxiv - Bioengineering 2023Quote: A549 cells encapsulated in hydrogels were stained at the beginning (Day 7) and the end of culture (Day 28) with 2 µM Calcein-AM (Invitrogen) and 30 µg/mL propidium iodide (PI ...
-
bioRxiv - Bioengineering 2024Quote: Reactive oxygen species (ROS) were quantitatively and qualitatively determined using the cell permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (ThermoFisher Scientific). H2DCFDA is a type of fluorescein that has been chemically reduced and is used to detect the generation of reactive oxygen intermediates in neutrophils and macrophages ...
-
bioRxiv - Cell Biology 2023Quote: ... cells were cultured for 7 days in Tu 2% media (1205Lu) supplemented with lipid depleted FBS (Omega Scientific, Fisher Scientific). Dox inducible LBR KD was initiated 3 days prior to the experiment ...
-
bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
-
bioRxiv - Immunology 2022Quote: ... membranes were washed three times with PBS-T and then incubated with appropriate secondary antibody conjugated with alkaline phosphatase that provides a visual color change upon addition of the chromogenic substrate (mixture of BCIP (5-bromo-4chloro-3-indolyl phosphate-catalog# 34040) and NBT (nitro-blue tetrazolium chloride, catalog# 34035 from Thermo Scientific)).
-
bioRxiv - Immunology 2022Quote: ... the captured viral biotinylated peptides were detected with 1 mg/ml pNPP (p-nitro-phenyl-phosphate, Thermo Fisher Scientific, cat: 34045) diluted in Diethanolamine substrate buffer 1X (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2021Quote: ... The final wash was followed by the addition of Nitro-blue Tetrazolium Chloride/5-bromo-4-chloro 3 ‘indolyl phosphate p-toludine salt (NBT/BCIP chromagen) substrate solution (Thermo Scientific) for 7 min ...
-
bioRxiv - Genomics 2020Quote: ... the upper aqueous phase was recovered and nucleic acids were precipitated by adding 2 μL GlycoBlue (Ambion) and 500 μL isopropanol (incubated at −20°C for 10 min) ...
-
bioRxiv - Genetics 2021Quote: ... After an additional 2 PBS washes cells were counterstained with DAPI nucleic acid stain (Thermo Fisher Scientific), diluted 1:1000 with PBS ...
-
bioRxiv - Bioengineering 2021Quote: ... buffered with 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... stock solutions were made in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The thrombin-selective inhibitor PPACK.2HCl and MMP Inhibitor V (ONO-4817 ...
-
bioRxiv - Synthetic Biology 2020Quote: ... we used 20 amino acids that contained 2 mM of 13C6 15N2 L-lysine (Thermo Fisher Scientific) and 13C6 15N4 L-arginine (Thermo Fisher Scientific ...
-
bioRxiv - Biochemistry 2022Quote: ... 1% nonessential amino acids (1140050) and 50μM 2-mercaptoethanol (31350-010) (all Thermo Fisher Scientific unless specified), 1,000 U/ml LIF (ESGRO ESG1107 ...
-
bioRxiv - Zoology 2023Quote: ... and 2-(N-morpholino)ethanesulfonic acid (MES) running buffer solution (Bolt™, Thermo Fisher Scientific, Waltham, Massachusetts). A standard ladder (Mark12™ ...
-
bioRxiv - Microbiology 2023Quote: GAG was subjected to acid hydrolysis with 2 M HCl (Gibco, Thermo Fisher Scientific, Waltham, MA, USA), 100 °C for 3 h to obtain soluble oligosaccharides of various length (max ...
-
bioRxiv - Cancer Biology 2023Quote: ... Cells were maintained in RF10: RPMI 1640 supplemented with 2 mM MEM nonessential amino acid solution (Gibco), 100 mM HEPES (Gibco) ...
-
bioRxiv - Biochemistry 2022Quote: ... Dried digests were reconstituted in 2% acetonitrile/0.1% formic acid and 0.3μg injected onto a LC pre-column (ThermoFisher Scientific Acclaim Pep map 100 ...
-
bioRxiv - Systems Biology 2023Quote: ... 0.1 mM CaCl2 and either 10% Luria Broth (Figs 1&2) or MEM amino acids (from Gibco) (Figs 3–5) ...
-
bioRxiv - Cancer Biology 2019Quote: ... essential amino acids and non-essential amino acids (MEM Non-Essential Amino Acids Solution; Gibco, 11140050) (amino acid-only medium) ...
-
bioRxiv - Immunology 2023Quote: ... Dead cells were excluded using 7-AAD (7-Aminoactinomycin D, Thermo Fisher Scientific, 00-6993-50). Cells were analyzed using a FACSCanto II flow cytometer (BD ...
-
bioRxiv - Microbiology 2021Quote: ... The recommended quantity of purified nucleic acids were amplified using real time PCR kits for PCV-2 (LSI VetMAX™ Porcine Circovirus Type 2 Quantification, Thermo Fisher Scientific, MA, USA), Porcine reproductive and respiratory syndrome virus [PRRSV] European and North American strains (LSI VetMAX™ PRRSV EU/NA Real-Time PCR Kit ...
-
bioRxiv - Molecular Biology 2021Quote: ... in QuantStudio 7 Flex (Applied Biosystems), in accordance with the manufacturer’s instructions ...
-
bioRxiv - Molecular Biology 2021Quote: ... 7 μL of RNaseI (Invitrogen AM2249) was added to the clarified lysates and digestion was done for 1 h at 4 °C ...
-
bioRxiv - Immunology 2021Quote: ... on QuantStudio 7 Flex (Applied Biosystems). For quantification of gene expression ...
-
bioRxiv - Bioengineering 2019Quote: ... 7′-dichlorodihydrofluorescein diacetate (cm-H2DCFDA, Invitrogen) for 60 minutes ...
-
bioRxiv - Cell Biology 2019Quote: ... or QuantStudio 7 Flex (Applied Biosystems). Refer to Supplemental Table 5 for primer sequences used.
-
bioRxiv - Cell Biology 2020Quote: ... or QuantStudio 7 Flex (Applied Biosystems). All mRNA expression levels were calculated relative to the housekeeping gene ...
-
bioRxiv - Synthetic Biology 2021Quote: ... 7% KnockOut SR (vol/vol) (Gibco), 2 mM L-glutamine ...
-
bioRxiv - Developmental Biology 2020Quote: ... 7% KnockOut SR (vol/vol) (Gibco), 2mM L-glutamine ...