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Citations for Thermo Fisher :
1 - 50 of 5154 citations for 7 bromoquinoline 8 thiol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... LMW thiols were quantified using Thiol Fluorescent Detection Kit (Invitrogen) according to the manufacturer’s instructions and were normalized by protein content.
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bioRxiv - Bioengineering 2023Quote: The efficiency of the norbornene-thiol reaction was measured using a thiol quantification assay (Measure-iT Thiol Assay Kit, Invitrogen).37 The cell adhesive ...
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bioRxiv - Microbiology 2020Quote: ... Thiol content was then measured using the Thiol Fluorescent Detection Kit (Invitrogen), according to the manufacturer’s instructions.
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bioRxiv - Biophysics 2021Quote: Thiol-reactive fluorescent dyes (ThermoFisher Scientific) were used for site-specific labeling of cysteines in MM variants ...
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bioRxiv - Immunology 2023Quote: Intracellular reduced glutathione levels were determined using commercially available fluorometric thiol probe thiol tracker Violet (Thermo Fisher) and the total glutathione (GSH ...
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bioRxiv - Biochemistry 2023Quote: ... Resuspended samples were subjected to reduced thiol quantitation using the Measure-iT™ Thiol Assay Kit (ThermoFisher) against reduced glutathione following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2020Quote: ... −7 and −8 was standard KSR/KODMEM (Life Technologies) medium whilst MasterShef-10 and −11 were derived in Nutristem medium (Biological Industries) ...
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bioRxiv - Microbiology 2023Quote: ... Green fluorescence emission is indicative of the thiol-reactive chloromethyl group of the CM-H2DCFDA probe reacting with glutathione and other thiols (Invitrogen).
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bioRxiv - Bioengineering 2023Quote: ... with bifunctional thiol dithiothreitol (DTT, Thermo Fisher Scientific) or a peptide crosslinker that was either broadly protease-degradable (KKCGGPQGIWGQGCKK ...
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bioRxiv - Immunology 2021Quote: ... and 8 μM of CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) for 30 min at 37°C 5% CO2 ...
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bioRxiv - Plant Biology 2020Quote: ... Labeled thiols were detected using a fluorescence detector 3400 RS (ThermoFisher) at 380 nm for excitation and 480 nm for emission.
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bioRxiv - Biophysics 2021Quote: ... Methyl-PEG4-thiol (MT(PEG)4) was purchased from Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: Thiol-reactive Tb3+ chelate DTPA-cs124-EMPH (Lanthascreen, Life Technologies, Inc.) was used as the LRET donor for all samples and Bodipy FL maleimide (Invitrogen ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Biochemistry 2023Quote: ... with 8 kD dialysis membrane (SpectraPor 7 8000 Dalton MWCO; 11425919, Fisher Scientific UK). For syntaxin-6 the buffer used for dialysis was 10 mM Na-phosphate,150 mM NaCl ...
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bioRxiv - Cell Biology 2022Quote: ... Reduced thiol groups were alkylated using 40 mM iodoacetate (IAA; Thermo Fisher) for 30 minutes at room temperature in the dark ...
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bioRxiv - Biochemistry 2021Quote: ... the thiol-reactive dye Alexa Fluor 488 C5 Maleimide (Thermo Fisher Scientific) was dissolved in dimethyl sulfoxide (DMSO ...
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bioRxiv - Biophysics 2022Quote: ... was filled with 7-8 μL of sample and sealed with Critoseal (Fisherbrand, Fisher Scientific) at one end ...
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bioRxiv - Bioengineering 2019Quote: ... The thiol functional group was conjugated with a Dylight 488 maleimide probe (Invitrogen), which was applied at a concentration of 10 μg/ml in PBS ...
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bioRxiv - Bioengineering 2022Quote: ... we used Tetra-PEG-SH fluorescently labeled with a thiol-reactive probe (Thermo Fisher Scientific or Merck KGaA ...
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bioRxiv - Physiology 2022Quote: ... reduced thiols were alkylated with biotin polyethyleneoxide iodoacetamide (Thermo Fisher Scientific, Waltham, MA, USA) for 2 hours on ice in ultrasound bath ...
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bioRxiv - Biochemistry 2021Quote: ... Freshly prepared, ice-cold RIPA buffer (110 μL, 50mM Tris, pH 7-8 (Acros Organics #14050-0010), 150 mM NaCl (Fluka #71383) ...
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bioRxiv - Immunology 2024Quote: ... Frozen lungs were cut into 7-8 µm sections using a Cryostar NX50 cryostat (Thermo Fisher Scientific) and mounted on silane-treated glass slides (Sigma-Aldrich) ...
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bioRxiv - Immunology 2020Quote: ... to reduce the oxidized thiols and EZ-Link Maleimide-PEG2-Biotin (Mal-PEG2-Bio, Thermofisher) to label the newly reduced thiols (Fig ...
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bioRxiv - Biophysics 2021Quote: ... Orb2B and Orb2BΔRBD were labeled with thiol-reactive Oregon Green 488 through maleimide chemistry (Thermofisher). Due to the lack of cystine residues in the Orb2AΔRBD protein construct ...
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bioRxiv - Biophysics 2022Quote: ... and completed after approximately 5 h (Thiol-Reactive Probe Labeling Protocol provided by Thermo Fisher Scientific). Labeled peptides were purified by RP-HPLC XSelect C4 column using a gradient of 30-50% B over 42 min ...
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bioRxiv - Physiology 2021Quote: ... reduced thiols within proteins were alkylated with BODIPY FL maleimide (final concentration of 10 nM, ThermoFisher) for 30 min in the dark after permeabilization ...
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bioRxiv - Immunology 2023Quote: ... 7-Aminoactinomycin D (7-AAD) (Invitrogen) at 1:400 was used as a marker for dead cells ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Three tadpoles per stage (1, 2, 5, 7, and 8 weeks after hatching) were fixed in an RNAlater (Ambion) solution ...
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bioRxiv - Molecular Biology 2021Quote: ... Lysosomal ExRai AMPKAR was made by inserting PCR-amplified LAMP149 generated using primers 7-8 into HindIII (ThermoFisher FD0504)/BamHI-digested ExRai AMPKAR backbone ...
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bioRxiv - Microbiology 2019Quote: Huh-7 and C6/36 cells were grown in 8 well chambers (LAB-TEK®, Nalgene Nunc International, USA) for 24h ...
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bioRxiv - Neuroscience 2020Quote: ... 1 mM EDTA plus PIC) with 20 mM thiol-reactive methyl-methane thiosulfonate (MMTS, Thermo Fisher Scientific) to block free cysteine residues ...
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bioRxiv - Neuroscience 2022Quote: Differentiated cells on day 15 (8 days suspension culture plus 7 days adherent culture) were harvested and dissociated using TrypLE Select (Gibco). Cells were then passed through a 20 µm strainer and resuspended in 1 x PBS with 0.04% BSA buffer ...
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bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
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bioRxiv - Immunology 2020Quote: PBMC were seeded at a concentration of 7-8 × 106 cells/mL in RPMI 1640 medium without phenol red and glutamine (Gibco) supplemented with 10% heat-inactivated FBS (Gibco ...
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bioRxiv - Cell Biology 2022Quote: Lipid overlay assay was performed using PIP strips (nitrocellulose membrane spotted with 8 phosphoinositides and 7 other biologically relevant lipids) according to the manufacturer’s instructions (Thermo Scientific). Briefly ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Genetics 2023Quote: ... Quantitative real time PCR (n = 7–8 per genotype) was performed on an ABI 7300 Sequence Detection system (Applied Biosystems) using the PowerUp SYBR Green Master Mix Kit (Applied Biosystems).
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bioRxiv - Biochemistry 2024Quote: HEK293 cells with Fzd1/2/4/5/7/8 knocked out were provided by Michael Boutros (Voloshanenko et al., 2017) and maintained in DMEM (Gibco) supplemented with 10% fetal bovine serum (Gemini) ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... we used 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) or FxCycle™ PI/RNase Staining Solution (Thermofisher ...
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bioRxiv - Genomics 2020Quote: ... 7-Aminoactinomycin D (7-AAD) (1:200 dilution, ThermoFisher Scientific #A1310 ...
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bioRxiv - Neuroscience 2022Quote: ... 7-aminoactinomycin D (7-AAD, Thermo Fisher, A 1310) was added 1:50 as a cell death marker.
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bioRxiv - Genetics 2021Quote: ... 4sU-labelled RNA was thiol-specific biotinylated by addition of 200 μg EZ-link HPDP-biotin (ThermoFisher Scientific, 21341), biotinylation buffer (10 mM Hepes-KOH pH 7.5 and 1 mM EDTA ...
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bioRxiv - Developmental Biology 2020Quote: ... Genital ridges from 1-2 litters (7-8 embryos per litter) were dissected out and digested at 37°C for 3 min using TrypLE Express (Thermo Fisher Scientific). For E9.5 and E10.5 stages ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted from Arabidopsis leaf tissues grown under an 8-h photoperiod or cotyledons from etiolated seedlings grown in dark for 7 d by TRIzol (Thermo Fisher Scientific) followed by DNase treatment at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...