Labshake search
Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 7 Phenyl 1 heptanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Synthetic Biology 2021Quote: ... The final plasmids were assembled in a total volume of 20 µl using 100 or 200 ng of linearized ArEc-TDH3 or GR-306MP backbone with molar insert:plasmid ratio of 7:1 (116 or 281 ng of digested PCR product, respectively) and 1 µl of T4 DNA ligase (Thermo Fisher Scientific, catalog no. EL0011). The mixtures were incubated for 3 h at room temperature ...
-
bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... 2,7-Dichlorodihydrofluorescein diacetate (DCFH-DA),3,3’-dihexyloxacarbocyanine iodide [DiOC6(3)] and N-[4-[6-[(acetyloxy)methoxy]-2,7-difluoro-3-oxo-3H-xanthen-9-yl]-2-[2-[2-[bis[2-[(acetyloxy)methoxy]-2-oxoethyl]amino]-5-methylphenoxy]ethoxy]phenyl]-N-[2-[(acetyloxy)methoxy]-2-oxoethyl]-,(acetyloxy)methyl ester (Fluo-4 AM) were purchased from Molecular Probes (Invitrogen, Eugene, OR, USA). Agarose was obtained from Lonza (Walkersville ...
-
bioRxiv - Microbiology 2022Quote: ... per well were distributed in Ibidi® µ-slide 8-well chambered coverslips and stained with 0.8 μM of the membrane specific fluorescent styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64; Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 0 μM (control) ...
-
bioRxiv - Cell Biology 2019Quote: ... methoxy]-2-oxyethyl] amino]-5-methyl-phenoxy] ethoxy]phenyl-N-[2-[(acetyloxy) methoxy]-2-oxyethyl]-(acetyloxy) methyl ester (Fluo-4/AM) were from Molecular Probes (Invitrogen, Eugene, OR, USA). M ...
-
bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...
-
bioRxiv - Molecular Biology 2021Quote: ... was cultured in Dulbecco’ ss Modified Eagle Medium (DMEM) supplemented with 7% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco) at 37°C with 5% CO2.
-
bioRxiv - Neuroscience 2020Quote: ... Replicates of hiPSC-derived neurons were harvested at three different timepoints of differentiation (days 1, 3, and 7) with 200 µl Trizol (Invitrogen) per sample and stored at −80 °C until sequencing ...
-
bioRxiv - Molecular Biology 2021Quote: ... Reactions with RNase T2 were done at room temperature for 10 minutes in 1× Structure Buffer (10 mM Tris pH 7, 100 mM KCl and 10 mM MgCl2; Ambion). Nuclease S1 cleavage was carried out for 15 minutes at room temperature in 1× Reaction Buffer (40 mM sodium acetate pH 4.5 ...
-
bioRxiv - Genetics 2022Quote: ... were combined and concentrated to ∼1 mL and dialyzed using 7 KMWCO Snake Skin dialysis tubing (Thermo Scientific, Waltham, Massachusetts) into 20 mM Tris 150 mM NaCl pH 8.4 ...
-
bioRxiv - Biochemistry 2021Quote: 3 biological replicates of wild type and 2 different clones METTL5-KO HeLa cells (clone 1 and 7) were grown to confluency prior to lysis in Trizol (Invitrogen). Total RNA was purified as described above (‘RNA purification’ ...
-
bioRxiv - Neuroscience 2021Quote: Astrocytes at 3 DIV or at 7-8 DIV after lentivirus infection were loaded with 1 μg/ml Fura-2-AM (#F1221, ThermoFisher) in astrocyte culture medium for 30 min at 37 °C ...
-
bioRxiv - Biophysics 2023Quote: ... cells were incubated with a dye master mix containing CellEventTM Caspase 3/7 Green Detection Reagent (ThermoFisher Scientific, 1:1000) and Zombie NIR fixable viability stain (BioLegend ...
-
bioRxiv - Microbiology 2023Quote: ... Five to 10 μg of total RNA were separated on a 7% (v/v) polyacrylamide gel (7.3 M urea, 1× TBE) and compared to RiboRuler LowRange RNA ladder (ThermoFisher Scientific). RNA from the gel was then blotted on a nylon membrane (Roche ...
-
bioRxiv - Cancer Biology 2023Quote: ... organoids developed within 7 - 21 days after initial seeding and were first passaged (1:2) by dissociation using TrypLE Express Enzyme (ThermoFisher) and resuspension in OcellO primary organoid medium ...
-
bioRxiv - Immunology 2024Quote: ... or 96-well flat-bottom plates at a concentration of 7 × 105 – 1 × 106 T cells/mL in T cell media with 10 U/mL rhIL-10 (Gibco). For both workflows ...
-
bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
-
bioRxiv - Neuroscience 2021Quote: ... Plates were then run on either a Viia™ 7 or QuantStudio™ 7 Flex Real-Time PCR Systems (both Applied Biosystems™) with 40 repetition of data collection steps at 60 °C ...
-
bioRxiv - Immunology 2024Quote: ... Real-time RT PCR was performed using ABI ViiA 7 and ABI QuantStudio 7 Flex Systems with MicroAmp® Optical 384-Well Reaction plates with specific barcodes (Life Technologies 4309849) and Power SYBR™ Green PCR Master Mix (Life Technologies 4368708) ...
-
bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
-
bioRxiv - Immunology 2020Quote: Total RNA was extracted and purified from synovial tissue (a total of 7 samples from 7 different RA patients) and cultured cells using Trizol reagent (Life Technologies, Carlsbad, CA, USA), followed by cDNA synthesis using a PrimeScript RT Reagent Kit with gDNA Eraser (Takara-Bio ...
-
bioRxiv - Microbiology 2023Quote: ... niveus CO7 DNA ranging from 7 to 7 × 10−4 ng/µl was generated and quantified using a Nanodrop-8000 Spectrophotometer (Thermo Scientific, Wilmington DE, USA) (260/280 ratio of 1.89) ...
-
bioRxiv - Plant Biology 2019Quote: ... Genotyping was performed on a Quant Studio 7 (Applied Biosystems) using KASP technology (LGC ...
-
bioRxiv - Neuroscience 2021Quote: ... and a ViiA 7 Real Time PCR System (Applied Biosystems). For each primer pair in a given experimental condition ...
-
bioRxiv - Biophysics 2021Quote: ... for days 0-7 and RPMI with B-27 (Gibco) on days 7-onwards ...
-
bioRxiv - Cancer Biology 2021Quote: ... in medium containing active caspase-3/7 detection reagent (ThermoFisher). Chamber slides were mounted on a heated stage within a temperature-controlled chamber maintained at 37°C ...
-
bioRxiv - Immunology 2021Quote: ... using a ViiA 7 Real-Time PCR System (ThermoFisher Scientific) as described(44) ...
-
bioRxiv - Cancer Biology 2020Quote: ... Data analysis was carried out using QuantStudio 7 (Applied Biosystems). Relative expression levels of target genes were calculated using the ΔΔCt method as described [12] with Actb ...
-
bioRxiv - Developmental Biology 2021Quote: ... Sections (7 um) were collected onto superfrost slides (Fisher Scientific). In situ hybridization was performed using the RNAscope multiplex in situ hybridization kit (Advanced Cell Diagnostics) ...
-
bioRxiv - Developmental Biology 2020Quote: ... and a ViiA 7 Real-Time PCR System (Applied Biosystems). The primer concentration used was 1 µM in a final reaction volume of 10µl ...
-
bioRxiv - Neuroscience 2022Quote: ... and ViiA™7 Real-Time PCR System (Life Technologies). PCR consisted of 40 cycles ...
-
bioRxiv - Molecular Biology 2021Quote: ... on a ViiA 7 Real-time PCR System (Applied Biosystems). Primer sequences used are listed in Table S13.
-
bioRxiv - Molecular Biology 2019Quote: ... supplemented with 7% (v/v) fetal bovine serum (Thermo Scientific) and 100 U/ml penicillin-streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2019Quote: ... on a ViiA-7 real-time thermal cycler (Applied Biosystems). All samples were analysed in technical duplicates ...
-
bioRxiv - Genomics 2019Quote: ... on a ViiA 7 Real-Time PCR System (Life Technologies). Gene expression levels were calculated relative to PPIA (cyclophilin A ...
-
bioRxiv - Cancer Biology 2019Quote: ... on a ViiA 7 Real-Time PCR System (Applied Biosystems). Relative mRNA levels were normalized to expression of human β-actin ...
-
Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... on a ViiA-7 Real-Time PCR system (Applied Biosystems). Determination of relative mRNA levels was calculated using the comparative 2-CT method [20] compared to the expression of housekeeping gene receptor like protein 32 (RPL32 ...
-
bioRxiv - Immunology 2020Quote: ... in a ViiATM-7 RT-PCR system (Thermo Fisher Scientific). Primer sequences are available upon request ...
-
bioRxiv - Immunology 2019Quote: ... and 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) were from Thermo Fisher Scientific ...
-
bioRxiv - Molecular Biology 2020Quote: ... on a ViiA 7 Real-Time PCR System (Applied Biosystems). Four technical replicates were averaged for each sample per primer reaction ...
-
bioRxiv - Microbiology 2019Quote: ... in a ViiA 7 Real-time PCR system (Applied Biosystems) for 40 cycles with two steps per cycle ...
-
bioRxiv - Cancer Biology 2019Quote: ... on the ViiA 7 Real-Time PCR System (Applied Biosystems) using the following primer pairs ...
-
bioRxiv - Physiology 2019Quote: ... RT-qPCR was performed on a QuantStudio 7 (Applied Biosystems) using Fast Taqman mastermix and the following probes (Applied Biosystems) ...
-
bioRxiv - Physiology 2019Quote: ... with 7 μL of PageRuler Plus Prestained (Thermo Fisher #26619) protein ladder in the first lane ...
-
bioRxiv - Cell Biology 2019Quote: ... qRT-PCR was performed on a ViiA 7 (Life technologies) using fast SYBR Green (Applied Biosystems) ...
-
bioRxiv - Pathology 2021Quote: ... qRT-PCR was performed on a ViiA 7 (Life technologies) using fast SYBR Green (Applied Biosystems) ...
-
bioRxiv - Genetics 2021Quote: ... in a Viia 7 RT-PCR machine from Applied Biosystems. All experiments were performed with least three biological replicates collected from different days ...
-
bioRxiv - Developmental Biology 2021Quote: ... Dead cells were stained with 7-AAD (A1310, ThermoFisher Scientific) and were excluded from collections ...
-
bioRxiv - Biochemistry 2021Quote: ... on a ViiA 7 Real-Time PCR System (Applied Biosystems). GAPDH expression functioned as internal control ...
-
bioRxiv - Microbiology 2021Quote: ... and the ViiA 7 real-time PCR system (Life Technologies). The oligonucleotide primers used for the qPCR analysis of gene expression are listed in Table S2 ...