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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 7 MethoxybenzoFuran 2 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... We labeled the purified RLC with 10 molar excess of 5-((((2-Iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (IAEDANS, Invitrogen) or dabcyl C2 maleimide (AnaSpec ...
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bioRxiv - Immunology 2022Quote: ... ileal tissue was collected and stored in stabilization buffer (2 mM ethylenediaminetetraacetic acid [EDTA; Invitrogen AM9260G], 2mM L-glutamine [Gibco 25-030] ...
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bioRxiv - Immunology 2019Quote: ... and eluted using a 500 μl/min flow rate over 10 min from 2 % buffer A to 35 % buffer B (0.1% formic acid in acetonitrile) with an Ultimate 3000 HPLC system (Thermo Scientific). One ml fractions were collected from 2 to 15 min ...
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bioRxiv - Neuroscience 2019Quote: ... Inducible overexpression of NGN2 began on day 1 by transferring cells into D0-2 induction medium (DMEM/F12, 1% Pen/Strep, 1x non-essential amino acid solution (NEAA, Gibco), 1% (v/v ...
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bioRxiv - Molecular Biology 2019Quote: ... We measured non-esterified fatty acids with HR Series NEFA-HR(2) (Wako Pure Chemicals) and triglycerides with Infinity #TR22421 (ThermoFisher).
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bioRxiv - Cell Biology 2019Quote: The mRFP-CLASP2γ U2OS cells expressing H2B-GFP were cultured in glass coverslips using DMEM without phenol red and supplemented with 25 mM of 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Gibco) and 10% FBS ...
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bioRxiv - Microbiology 2019Quote: ... Peptides from each fraction were reconstituted in 0.1% formic acid and loaded on a pre-column (PepMap C18, 100 μm × 2 cm, Thermo Scientific) at a flow rate of 5 μL per min ...
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bioRxiv - Pathology 2019Quote: ... or a custom diet high in fat (60 kcal%), cholesterol (1.25%) and cholic acid (0.5%) (D11061901, Research Diet, Denmark) as well as 2% CDX (Fisher Scientific, France) in drinking water (HFCC-CDX group).
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bioRxiv - Bioengineering 2020Quote: The quantification of BMP-2 initially loaded in the polyelectrolyte film (deposited at the bottom of a 96-well microplate) was done using a micro bicinchoninic acid assay (microBCA) test for low BMP-2 concentration (BMP20) while Nanodrop (Thermofisher) was used for higher concentrations ...
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bioRxiv - Bioengineering 2021Quote: ... T cells were cultured at 2×106 cells/ml in T cell media (R10 supplemented with 1X non-essential amino acids (Gibco) + 1 mM sodium pyruvate (Gibco ...
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bioRxiv - Biochemistry 2022Quote: ... the samples were re-suspended in 2 % formic acid and analysed using an UltiMate™ 3000 RSLCnano System (ThermoFisher Scientific) coupled on-line to an Orbitrap Exploris 480 (ThermoFischer Scientific) ...
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bioRxiv - Genetics 2022Quote: ... The peptides were dissolved in 2% formic acid and analysed on a Q-Exactive HF mass spectroscopy (Thermo Fisher Scientific). The peptides were separated using an EASY-Spray PepMap RSLC C18 column at flow rate of 300 nL/minute on a 60 min linear gradient of the mobile phase (5% acetonitrile (ACN ...
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bioRxiv - Microbiology 2021Quote: Multiplexed peptide fractions from each time point were resuspended in mobile phase A solvent (2% acetonitrile and 0.1% formic acid) to be analyzed on the Exploris 480 mass spectrometer equipped with FAIMS (ThermoFisher Scientific). The mass spectrometer was interfaced to the Easy nanoLC1200 HPLC system (ThermoFisher Scientific) ...
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bioRxiv - Immunology 2019Quote: Samples were diluted in culture medium (RPMI 1640 [Gibco, Massachusetts/USA] supplemented with 2 g of NaHCO3, 10 mL of nonessential amino acids [Gibco] ...
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bioRxiv - Biochemistry 2020Quote: ... The liquid chromatography used a 2-90% MeCN linear gradient in water with 0.1% formic acid on a C18 BioBasic analytical HPLC column (Thermo Scientific) installed on an Agilent 1290 HPLC system ...
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bioRxiv - Biochemistry 2021Quote: We monitored the change in absorbance over time of 5,5’-dithiobis-(2-nitrobenzoic acid) (DTNB, Ellman’s reagent, Thermo Fisher Scientific). Various concentrations of DTNB were added to protein and change in absorbance was measured in either an SX-20 Stopped Flow instrument (Applied Photophysics ...
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bioRxiv - Immunology 2020Quote: ... the desalted samples were first resuspended into 20 μl 0.1% formic acid in water and then loaded onto a trap column (2 cm × 300 μm, PepMap C18, Thermo Scientific) and an analytical column (19 cm × 75 μm ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were trapped at 3 μl/min on a 10 mm × 75 μm ID Acclaim Pepmap trap at 2% buffer B (buffer A: 0.1% formic acid (Fisher Scientific), buffer B ...
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bioRxiv - Molecular Biology 2022Quote: ... Peptides were then dissolved in 2% ACN and 0.1% formic acid and analyzed on a Thermo Scientific Q Exactive Mass Spectrometer (ThermoFisher Scientific). The MS was operated in data-dependent mode ...
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bioRxiv - Molecular Biology 2022Quote: The reconstituted peptides in solvent A (2% acetonitrile, 0.1% formic acid) were separated on a 50 cm long EASY-spray column (ThermoFisher Scientific) connected to an Ultimate-3000 nano-LC system (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: ... DNA and RNA products at various points of the iTP-seq protocol were analyzed on 9% acrylamide (19:1) TBE (90 mM Tris, 90 mM boric acid, and 2 mM EDTA) gels and stained with SyBR Gold (Invitrogen). Inverse toeprints were excised from 12% acrylamide TBE gels using a clean scalpel ...
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bioRxiv - Cell Biology 2023Quote: ... were grown in high-glucose DMEM with supplements (1% penicillin/streptomycin, 10% FBS, 1% Non-essential Amino Acids [NEAA; Wako], 55 µM 2-Mercaptoethanol [Gibco]). Mouse embryonic stem (ES ...
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bioRxiv - Molecular Biology 2023Quote: ... Peptides were resuspended in 2% acetonitrile in 0.1% formic acid and loaded at 5 μL/minute onto a PepMap C18 trap column (Thermo Scientific; 5 μm 100 Å particle size ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% formic acid in acetonitrile) on an EASY-Spray column ES801 (50 µm ID × 15 cm, 2 µm; Thermo Scientific) mounted on a DPV ion source (New Objective ...
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bioRxiv - Biochemistry 2024Quote: ... was labeled with the fluorescent probe 5-((((2-iodoacetyl)amino)ethyl)amino)naphthalene-1-sulfonic acid (1,5-IAEDANS from Molecular Probes) as previously described (17 ...
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Macropinocytosis mediates resistance to loss of glutamine transport in triple-negative breast cancerbioRxiv - Cancer Biology 2024Quote: ... Cells were trypsinised and resuspended in FACS buffer (1ξ Ca2+ and Mg2+ free PBS, 2 mM ethylenediaminetetraacetic acid (Life Technologies), 2% FBS Bovogen) ...
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bioRxiv - Cancer Biology 2024Quote: Peptides were reconstituted with 2% formic acid and analyzed in triplicates on an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific) coupled to an UltiMate 3000 UHPLC system (Thermo Scientific) ...
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bioRxiv - Cell Biology 2021Quote: ... and a mix of amino-acids (1X MEM Non-Essential Amino Acids and 1X MEM Amino Acids, both manufactured by Gibco R). For strains and plasmid construction cells were grown in LB ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were cοtransfected with 7 µg CRISPR plasmid and 7 µg non-linearized HDR template using Lipofectamine 3000 according to the instructions (ThermoFisher). 48 hours after transfection ...
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bioRxiv - Microbiology 2022Quote: ... and the vesicular stomatitis virus G (VSV-G)-expressing plasmid at a ratio of 7:7:1 (5 μg total amount of DNA) using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... 5 mM EDTA and 1% BSA in HBSS) and 2μL of 7-aminoactinomycin D (7-AAD, Thermo Fisher Scientific, A1310) at a concentration of 0.1 mg/mL were added ...
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TAD border deletion at the Kit locus causes tissue-specific ectopic activation of a neighboring genebioRxiv - Genetics 2022Quote: ... To exclude cells with compromised membranes cell suspension was incubated with 7-aminoactinomycin D (7-AAD) (Thermo Fisher Scientific, A1310) in a ratio 1:1000 for at least 20 min ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 activity was assessed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Scientific, #C10427) according to the manufacturer’s protocol ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... samples were incubated with 5 µg/mL of the DyLight488-conjugated anti-FLAG M1 antibody and the viability dye 7-Aminoactinomycin D (7-AAD, Invitrogen) for 45 min on ice and were then analyzed on a Guava easyCyte cytometer (Millipore) ...
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bioRxiv - Cell Biology 2021Quote: Intracellular levels of reactive oxygen species (ROS) were measured using the cell-permeant redox-sensitive probe 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Molecular Probes, Thermo Fisher Scientific, USA). HEK 293 cells were grown in a 96-well plate for fluorescent-based assays until ∼80% confluence ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Fecal samples were immediately transferred to an anaerobic chamber within 2 h after defecation and suspended at 20% (w/v) in reduced peptone water (0.1%, pH 7; Thermo Fisher Diagnostics AG, Pratteln, Switzerland). Immobilization of 10 ml fecal slurry in polymer gel beads (gellan gum (2.5% ...
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bioRxiv - Cancer Biology 2023Quote: ... Tris-Glycine 10-20% or Bis-Tris 4-12% SDS-PAGE gels were run at 4℃ and proteins were transferred onto nitrocellulose membranes using 15 Volts for 7 minutes via the iBlot-2 system (Thermo Fisher Scientific, Waltham, MA). The membrane was then blocked for 1 hour in LICOR Odyssey blocking buffer and incubated at 4℃ with the appropriate antibody overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were incubated with 2.5 µM DCFH-DA (2’,7’-dichloro-dihydro-fluorescein-diacetate; excitation at 495 nm, emission at 520 nm; Life Technologies, Europe BV, Stockholm, Sweden) in Hank’s buffered saline solution without phenol red for 30 min at 37°C ...
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bioRxiv - Cancer Biology 2019Quote: ... 4 mM serine and essential amino acids (MEM Amino Acids Solution; Gibco, 11130036); for all other experiments ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Hydrochloric acid and acetic acid were provided by Fisher Scientific (Loughborough, United Kingdom). 17α-methyltestosterone was delivered by Steraloids (Newport ...
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bioRxiv - Cell Biology 2019Quote: ... on a QuantStudio 7 Flex instrument (Applied Biosystems). Relative gene expression was calculated using the ΔΔCq method and normalized to GAPDH expression ...
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bioRxiv - Molecular Biology 2020Quote: ... and QuantStudio 7 Flex thermal cycler (ThermoFisher; 4485701). Each qRT-PCR was repeated at least three times ...
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bioRxiv - Molecular Biology 2019Quote: ... MCF-7 cells were grown in DMEM (GIBCO) with phenol red ...
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bioRxiv - Neuroscience 2019Quote: ... and CellEvent caspase-3/7 reagent (ThermoFisher Scientific) were used according to manufacturer protocol ...
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bioRxiv - Developmental Biology 2021Quote: ... followed by incubation with 7-AAD (Thermo Fisher) for viability assessment ...
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bioRxiv - Neuroscience 2020Quote: ... and with 7-AAD live/dead stain (Invitrogen) at a concentration of 1:50].
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bioRxiv - Cancer Biology 2021Quote: ... MCF-7 cells were cultured in DMEM (Gibco) supplemented with 10% FBS and 500IU/mL penicillin and 500μg/mL streptomycin (Sigma) ...
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bioRxiv - Genomics 2021Quote: ... MCF-7 were grown in DMEM (Gibco,11995065) supplemented with 10% FBS (Gibco,10099141) ...
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bioRxiv - Genomics 2022Quote: ... MCF-7 were grown in DMEM (Gibco,11995065) supplemented with 10% FBS (Gibco,10099141) ...
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bioRxiv - Immunology 2022Quote: ... and Cell Event Caspase-3/7 Green (Invitrogen) were loaded at the final concentration of 5 µM and 7 µM respectively along with the cells ...