Labshake search
Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 7 FLUORO 1H INDOL 3 METHYLAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2023Quote: ... for 1h at 37°C and then with Proteinase K (Fisher Scientific, #25-530-049) for 2h at 55°C ...
-
bioRxiv - Neuroscience 2023Quote: ... they were probed for 1h at RT with the fluorescent secondary antibodies (1:1000: ThermoFisher). Cell nuclei were stained with Hoechst 33342 (10 μg/mL ...
-
bioRxiv - Genetics 2023Quote: ... transiently transfected HEK293T cells were starved for 1h in methionine-free RPMI (Thermo Fisher Scientific) and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer) ...
-
bioRxiv - Genomics 2024Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
-
bioRxiv - Microbiology 2023Quote: ... for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (SNN1008, Invitrogen). Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI ...
-
Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... Mucociliary Transport (MCT) was assessed by automatically tracking the displacement of fluorescent microspheres (1 μm diameter, Fluoro Max Dyed Green, G0100, ThermoFisher, Waltham, MA) added to the surface of live ALI cultures by using the Fiji plugin TrackMate (38) ...
-
bioRxiv - Biophysics 2019Quote: ... 0.2 μM Rhodamine B solution was used to align the confocal pinhole and 50 nm fluorescent polystyrene particles (Fluoro-Max Fluorescent Polymer Microspheres, 1% solid, Thermo Fisher Scientific) was prepared in de-ionized water as reference standard (D = 9.5 × 10−8 cm2/s in water ...
-
bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
-
bioRxiv - Evolutionary Biology 2020Quote: ISH and dFISH were performed as previously described (3, 7, 62) with addition of T1 RNAse treatment (2 u/µl, Thermo Fisher Scientific, Waltham, MA, USA) after probe washing in embryos older than 4 dpf to reduce background staining ...
-
bioRxiv - Bioengineering 2021Quote: Human lung fibroblasts (hTERT T1015, abmgood) were used between passages 7-12 and culture medium was changed every 2-3 days (Gibco Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10 v/v% fetal bovine serum (FBS ...
-
bioRxiv - Immunology 2021Quote: ... To obtain Mfs purified monocytes were plated on 24 well plates 3 × 105 cells/well and cultured for 7 days in Macrophage-SFM (Gibco, Life Technologies, Grand Island, NY, USA) with recombinant human GM-CSF (Miltenyi Biotech ...
-
bioRxiv - Immunology 2020Quote: ... The sorted germinal centre B cells (7-AAD−/B220+/GL-7+/Fas+) were digested with Proteinase K (Invitrogen) at 55°C overnight ...
-
bioRxiv - Cell Biology 2022Quote: ... 110 nm ultrathin cryosections were collected on 7×7 mm silicon wafers with fluorescent beads (PS-Speck, ThermoFisher). Light microscopy images were acquired with a widefield microscope ...
-
bioRxiv - Cell Biology 2020Quote: ... for 1h at room temperature before rinsing and incubation with streptavidin-HRP (SNN1004, Invitrogen, 1:5000) 30 min at room temperature ...
-
bioRxiv - Cell Biology 2020Quote: ... After incubation for 1h with the appropriate secondary antibodies (Alexa Fluor 488 or 594 (Thermo Fisher)) ...
-
bioRxiv - Molecular Biology 2020Quote: ... and incubated for 1h on ice with or without RNase (10μg/mL RNase A) (Thermo Fisher). Lysate was centrifuged at 4°C ...
-
bioRxiv - Evolutionary Biology 2022Quote: Cells were harvested via centrifugation at 21,000xg 4°C 1h (Thermo scientific, Sorvall Lynx 4000 centrifuge), the supernatant discarded and the resulting pellet resuspended in 1ml of spent medium ...
-
bioRxiv - Cell Biology 2022Quote: ... followed by PBS washes (3x) and staining with Hoechst (Life Technologies, 1h, 1:10000 in PBS). Cells were imaged using a 20x water immersion objective on a high content confocal microscope (Molecular Devices™ ImageXpress Micro XL).
-
bioRxiv - Synthetic Biology 2019Quote: ... cells were incubated with a goat anti-rabbit secondary antibody (Alexa 633, Invitrogen; 1:100, 1h) and then analysed by a Fluorescence Activated Cell Sorter (Aria III ...
-
bioRxiv - Physiology 2021Quote: ... The reactions were then incubated for 1h at 4°C with protein A/G dynabeads (Invitrogen) pre-blocked in PBS/0.5% BSA/0.5% Tween ...
-
bioRxiv - Molecular Biology 2021Quote: ... for 1h followed by incubation with goat α-rabbit Alexa Flour 488 (Thermo Fisher, A-11008) for 1h ...
-
bioRxiv - Cell Biology 2022Quote: ... and goat anti-mouse Alexa Fluor 488 secondary antibody (1:500, 1h; A-21121, Invitrogen, Germany) were used ...
-
bioRxiv - Genomics 2022Quote: ... primary incubation was performed for 1h (anti-fibrillarin monoclonal mouse, 1:100, MA3-16771, Thermo Scientific), followed by incubation with secondary antibody (anti-mouse monoclonal FITC ...
-
bioRxiv - Developmental Biology 2023Quote: ... after 1h of secondary antibody incubation fluorochrome conjugated phalloidin (Alexa Fluor™ 546 Phalloidin, Invitrogen™) was added to the secondary antibody solution for F-actin membrane staining for another hour ...
-
bioRxiv - Cancer Biology 2023Quote: ... Each coverslip was then incubated for 1h at 37ºC with Duolink PLA probes (Thermo Fisher Scientific) diluted in blocking solution ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
-
bioRxiv - Cancer Biology 2023Quote: ... 4X washes in PBS and 1h incubation with secondary antibody Alexa 488 goat anti-rabbit (Invitrogen) followed by 4X additional washes in PBS ...
-
bioRxiv - Immunology 2024Quote: ... overnight at 4°C and the relevant secondary for 1h at room temperature (Streptavidin-AF488 Invitrogen S11223 at 2 µg/mL final ...
-
bioRxiv - Cell Biology 2020Quote: ... using the ViiAtm 7 platform (Applied Biosystems). Relative quantification was conducted by either SYBRgreen fluorescent dye (Applied Biosystems ...
-
bioRxiv - Cell Biology 2019Quote: ... 7 kDa cut-off (Thermo Scientific, #89890). The protein concentration was adjusted to 0.5 mg/ml in CD buffer (250 μL) ...
-
bioRxiv - Genomics 2020Quote: ... 7 mM MgCl2 (Ambion, Cat. No. AM9530G), 10% N,N-dimethylformamide (Sigma ...
-
bioRxiv - Immunology 2022Quote: ... on the ViiA 7 system (Applied Biosystems). PCR of CD19 was used for normalization ...
-
bioRxiv - Immunology 2022Quote: ... on the ViiA 7 system (Applied Biosystems) as described (62 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 7 µL HinFI (10U/µL, Thermo Scientific)) per 70 µL of cDNA and digested at 37°C for 30 minutes ...
-
bioRxiv - Microbiology 2020Quote: ... and a QuantStudio 7 Flex (Life Technologies) instrument ...
-
bioRxiv - Microbiology 2021Quote: ... QuantStudio 6/7 Pro systems (Applied Biosystems). The following primers were used ...
-
bioRxiv - Neuroscience 2020Quote: ... with a ViiA 7 System (Applied BioSystems). The UBE2D2 gene transcript was used as the internal reference ...
-
bioRxiv - Systems Biology 2021Quote: ... and 1:1000 7-AAD (ThermoFisher Scientific). Mouse IgG2A-488 (R&D systems ...
-
bioRxiv - Microbiology 2020Quote: ... and a QuantStudio 7 Flex (Life Technologies) instrument for Il17a (96) ...
-
bioRxiv - Immunology 2022Quote: ... and 7-AAD (Invitrogen, 00-6993-50). CD19/CD20+ ...
-
bioRxiv - Neuroscience 2022Quote: ... Potassium Chloride (Fisher Scientific- 7447-40-7) and Calcium chloride (Fisher Scientific- 10043-52-4).
-
bioRxiv - Neuroscience 2022Quote: ... for 7 days in Neurobasal medium (Invitrogen) with 2% B-27 supplement (Invitrogen) ...
-
bioRxiv - Bioengineering 2022Quote: ... 7 mL of DMEM (Gibco, #11995-073) with 630 U/mL Type 1A collagenase from clostridium histolyticum (Sigma ...
-
bioRxiv - Cancer Biology 2022Quote: ... Real-time PCR (Applied Biosystems, #ViiA 7) was performed using the KAPA SYBR FAST qPCR master Mix (KAPA Biosystems ...
-
bioRxiv - Immunology 2019Quote: ... IL-7 (5 ng ml−1; ThermoFisher), and IL-15 (5 ng ml−1 ...
-
bioRxiv - Neuroscience 2021Quote: ... HT-7 (1:200, #MN1000, Thermo Scientific), GPC-4 (1:200 ...
-
bioRxiv - Biochemistry 2020Quote: ... 7-hydroxycoumarin was obtained from Acros Organics, a part of Thermo Fisher Scientific (New Jersey ...
-
bioRxiv - Bioengineering 2019Quote: ... IL-7 at 5 ng/mL (ThermoFisher), and IL-15 at 5 ng/mL (ThermoFischer) ...
-
bioRxiv - Cell Biology 2020Quote: ... on QuantStudio 7 Flex system (Life Technologies). To quantify viral genomes ...
-
bioRxiv - Cell Biology 2020Quote: ... Claudin 7 (Fisher Scientific 10537403, 1:200), Large T (Santa Cruz sc-20800 ...