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Citations for Thermo Fisher :
351 - 400 of 10000+ citations for 7 Benzyl 2 7 Diazaspiro 3.5 nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: CellEvent® Caspase 3/7 Green (Thermo Fisher, UK) was used to evaluate cell apoptosis ...
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bioRxiv - Cancer Biology 2023Quote: ... the CellEvent Caspase-3/7 Green Detection kit (Invitrogen) was used with propidium iodide as a viability marker ...
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bioRxiv - Cell Biology 2023Quote: ... Dead cells were stained with 7-AAD (ThermoFisher: A1310) and removed with cell sorter (SONY ...
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bioRxiv - Microbiology 2023Quote: ... and DSP1-7 plasmids using Lipofectamine 3000 (Thermo Scientific) according to manufacturer’s instructions in triplicates ...
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bioRxiv - Cancer Biology 2023Quote: ... and Quantstudio 7 flex RT-PCR system (ThermoFisher Scientific). Expression was measured and the ΔCT was calculated ...
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bioRxiv - Biophysics 2022Quote: COS-7 cells were cultured in DMEM (GIBCO, 21063029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Plant Biology 2023Quote: ... and ViiA 7 Real-Time PCR machine (Thermofisher, USA) was employed ...
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bioRxiv - Bioengineering 2023Quote: ... 1:500 of 7-AAD (Invitrogen, #00-6993-50) nuclei staining solution was added and nuclei were gated from the debris (Supplementary Figure 7 ...
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bioRxiv - Cancer Biology 2023Quote: ... and 7-AAD viability staining solution (Thermo Fisher Scientific), after treatment with a human FcR blocking reagent (Miltenyi Biotec) ...
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bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems). Transcript RNA levels were normalized against β-actin reference gene following the 2-ΔCt method ...
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bioRxiv - Molecular Biology 2023Quote: ... and ViiA-7 Real-Time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2023Quote: ... blocked with 7% goat serum (Gibco; Thermo Fisher Scientific), and incubated with primary antibodies followed by incubation with fluorescent secondary antibodies (Table 2) ...
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bioRxiv - Developmental Biology 2023Quote: ... CellEvent Caspase3/7 Green detection reagent (Thermo-Fisher Scientific) was used to label cells with caspase activity 30 minutes prior to fixation ...
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bioRxiv - Immunology 2023Quote: ... through ViiA-7 Fast Real-Time System (Applied Biosystems). Data were processed using ViiA™ 7 Software (Applied Biosystems ...
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bioRxiv - Cell Biology 2023Quote: ... blocked with 7% goat serum (Gibco; Thermo Fisher Scientific), and incubated with primary antibodies followed by incubation with fluorescent secondary antibodies (Table 2) ...
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bioRxiv - Immunology 2023Quote: ... 5 μl of 7-AAD (Invitrogen, 00-6993-50) was included for dead cell exclusion.
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bioRxiv - Cell Biology 2023Quote: CellEvent™ Caspase-3/7 Green Detection Reagent (Invitrogen) (1:500 ...
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bioRxiv - Microbiology 2023Quote: ... 7% Fetal bovine serum (FBS)(Cat# 26140079; ThermoFisher Scientific), and 100 U/mL penicillin-streptomycin (Cat# 15140122 ...
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bioRxiv - Cell Biology 2023Quote: ... Viability was controlled using 7-Aminoactinomycine D marker (Invitrogen). Fluorescence intensity of CTV and antibodies was measured with the SP6800 cytometer (Sony ...
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bioRxiv - Genetics 2023Quote: ... A clean glass slide (Fisher Scientific, 12-544-7) was dipped into freshly made PFA solution (1% paraformaldehyde adjusted to pH 9.2 using 1.25 M sodium borate ...
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bioRxiv - Immunology 2023Quote: ... on a ViiA 7 Real-Time PCR System (ThermoFisher) using the primers listed in supplementary methods ...
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bioRxiv - Genomics 2024Quote: ... RT-qPCR was carried on QuantStudio 7 Flex (ThermoFisher). Fold change differences were plotted as 2-ΔCt.
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bioRxiv - Neuroscience 2024Quote: ... and ran on a QuantStudio 7 Flex (Thermo Fisher). The RNase P TaqMan Copy Number Reference Assay was used for normalization within the same sample ...
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bioRxiv - Molecular Biology 2024Quote: Transient transfection of the 7 miRNA mimics (Dharmacon, ThermoFisher) or the miRNA control (miR-CTRL-1 ...
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bioRxiv - Microbiology 2024Quote: ... with the 7-AAD live/dead stain (Thermo Fisher) [104] with experiment performed over 3 hours ...
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bioRxiv - Molecular Biology 2024Quote: ... or 7% Tris-Acetate polyacrylamide gradient gels (Invitrogen, #EA0355BOX).
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bioRxiv - Biochemistry 2024Quote: ... anti-p53 (Invitrogen, monoclonal antibody DO-7 # MA5-12557), anti-CHOP (Cell Signaling ...
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bioRxiv - Cell Biology 2019Quote: ... Caspase-3/7 activation in live cells was monitored using CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific, C10423, 1:1000) in IncuCyte Live Cell Analysis System ...
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bioRxiv - Cancer Biology 2020Quote: Apoptosis was measured by caspase-3/7 staining using CellEvent® Caspase-3/7 Green ReadyProbes® Reagent (ThermoFisher Scientific Inc., cat# R37111). CHLA20 and NGP cells were grown until confluence on 6-well plates with six days of treatment with DMSO or GSK591 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were then run on either a Viia™ 7 or QuantStudio™ 7 Flex Real-Time PCR Systems (both Applied Biosystems™) with 40 repetition of data collection steps at 60 °C ...
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bioRxiv - Immunology 2024Quote: ... Real-time RT PCR was performed using ABI ViiA 7 and ABI QuantStudio 7 Flex Systems with MicroAmp® Optical 384-Well Reaction plates with specific barcodes (Life Technologies 4309849) and Power SYBR™ Green PCR Master Mix (Life Technologies 4368708) ...
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bioRxiv - Cancer Biology 2024Quote: ... 48hrs after transfection the cells were fixed and stained for activated Caspase-3/7 using Apoptosis CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) according to manufacturers’ instructions ...
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bioRxiv - Plant Biology 2020Quote: ... cinerea germlings exposed to different concentrations NCR044.1 was measured every 30 min for 2 h using the ROS indicator dye 2′,7′-dichlorodihydrofluorescein diacetate (H2DCF-DA, Invitrogen, Carlsbad, CA). ROS levels were quantified by measuring the fluorescence in a SpectraMax® M3 spectrophotometer microplate reader (exc ...
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bioRxiv - Physiology 2021Quote: ... HUVECs were washed 2 × with D-PBS and loaded with DAF-FM™ diacetate (4-amino-5-methylamino-2′,7′-difluorofluorescein diacetate; Molecular Probes, Invitrogen) to a final concentration of 1 µM in KRH buffer and incubated at 37°C for 45 minutes protected from light ...
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bioRxiv - Cell Biology 2022Quote: ... 2’,7’-Bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM) was purchased from Molecular Probes (Invitrogen, Carlsbad, CA, USA). Fluorescein isothiocyanate (FITC)- and tetramethylrhodamine (TRITC)-conjugated goat anti-mouse and rabbit IgG antibodies were purchased from Jackson ImmunoResearch (West Grove ...
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bioRxiv - Synthetic Biology 2019Quote: ... 1,2-dipalmitoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) was purchased from Invitrogen. Calcein dye ...
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bioRxiv - Neuroscience 2020Quote: NO production was detected by DAF-FM diacetate (4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate) (D23844, ThermoFisher). Fly larvae were dissected at 24 or 48 h AI in PBS to expose the sensory neurons ...
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bioRxiv - Cancer Biology 2021Quote: ... After 3 and 7 days of treatment cells were stained with 2% crystal violet (CV) (40583100, Acros Organics, Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... Pipettes had resistances of 5–7 MΩ when filled with this solution supplemented with Fura-2 (Molecular Probes). Recordings were made using a Multiclamp 700B amplifier (Molecular Devices ...
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bioRxiv - Microbiology 2020Quote: ... prehybridization was carried out for 2 h at 42°C in 7 mL of prehybridization buffer ULTRAHyb (Ambion). Hybridization was performed overnight at 42°C in the same buffer in the presence of a [γ-32P]-labeled DNA oligonucleotide probe ...
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bioRxiv - Microbiology 2021Quote: ... Unbound GTP was removed using 2 mL Zeba column with 7 kDa molecular weight cutoff (Thermo Scientific, 89889) and exchanged into 30 mM Tris pH = 7.5 buffer containing 1 mM DTT ...
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bioRxiv - Cell Biology 2022Quote: ... Nuclei were counterstained using 4′,6-diamidin-2-phenylindol (DAPI, 1:1000, 7 min; D1306, Thermo Fisher Scientific). Washing steps were performed with PBS ...
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bioRxiv - Microbiology 2023Quote: ... and then primers listed in Supplemental Table 2 on a ViiA 7 real-time PCR system (Applied Biosystems) with the following cycling parameters ...
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bioRxiv - Microbiology 2023Quote: ... the cells were loaded with 2 µM CellEvent™ Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific), according to the manufacturer’s instructions for kinetic assays and fluorescence in the live cells ...
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bioRxiv - Developmental Biology 2022Quote: ... Quantitative PCR for pri-let-7 and eft-2 were performed with Absolute Blue SYBR Green (Thermo Scientific) on the CFX63 Real Time System Thermocyclers (Biorad ...
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bioRxiv - Immunology 2023Quote: ... iGB cells were collected after 6-7 days on iGC culture and were stained with Fura-2 (Invitrogen) at 1μM / ml ...
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bioRxiv - Biophysics 2023Quote: ... The DTT was removed using a 2 ml ZebaSpin MWCO 7 kD desalting column (Thermo Fisher Scientific, USA). The columns were pre-equilibrated by washing them with 1 ml 10 mM TRIS buffer at pH 7.5 for three consecutive times by spinning them at 1000 G for 2 minutes for each washing step ...
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bioRxiv - Genomics 2022Quote: ... HPC-7 cells were maintained at density of 2-10 x105/ml in Iscove’s modified Dulbecco’s medium (Invitrogen) supplemented with 50 ng/ml of mouse stem cell factor (Gemini Bio-Products) ...
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bioRxiv - Biochemistry 2023Quote: ... for 40 min before neutralizing to approximately pH 7 with 2 M Tris (Thermo Fisher Scientific, Waltham, MA) in water ...
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bioRxiv - Cell Biology 2023Quote: ... HSPCs were incubated with 20µM NBD C6-Ceramide (6-((N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)amino)hexanoyl)Sphingosine) (Invitrogen) for 30 minutes at 4°C for Golgi staining or Cytopainter (Abcam ...