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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for 7 8 Dihydroisoquinolin 5 6H one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells were pelleted one last time at 400 x g for 5 min and resuspended into 5 ml of PBS (GIBCO) supplemented with protease inhibitors (ThermoFischer Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Genomics 2019Quote: ... To one portion 10 µL T4 DNA ligase (5 Weiss U/µL, Thermo Scientific) was added ...
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bioRxiv - Cancer Biology 2023Quote: ... and one wash with 50 μl of 5× Maxima H RT buffer (Thermo Fisher). Finally ...
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bioRxiv - Microbiology 2021Quote: ... 5 mM Aminoguanidine and 2.5 mM Sodium Ascorbate the reaction allowed to proceed for 6h at room temperature before removal of the biotin excess using Zeba desalting columns (7kDa cut-off, Thermofisher). The samples were then combined altogether at a 1:1:1 ratio within one replicate ...
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bioRxiv - Genomics 2024Quote: ... Cells were harvested for RNA extraction at 6h and 24h from unstimulated and stimulated PBMCs using TRIzol® (Life technologies) reagent according to manufacturer’s instructions and treated with DNase to ensure elimination of genomic DNA ...
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bioRxiv - Cell Biology 2020Quote: ... Sequencing was performed using Ion Proton instrument with 7 or 8 samples per chip with Ion PI Hi-Q Sequencing 200 Kit (ThermoFisher Scientific). Reads were aligned to the hg19 AmpliSeq Transcriptome ERCC v1 with Torrent Mapping Alignment Program (version 5.0.4 ...
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bioRxiv - Biophysics 2023Quote: ... the media was removed and the cells were incubated with 8 µM CellEventTM Caspase 3-7 green detection reagent (Thermo Fisher) in PBS containing 5% FBS for 30 min at 37 °C ...
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bioRxiv - Cancer Biology 2021Quote: ... carrying the mutation R273C was first amplified by PCR using the primers hp53-1 (5’-CACCATGGAGGAGCCGCAGTCAGATCC-3’) and hp53-8 (5’-GGATCCTCAGTCTGAGTCAGGCCCTTCTGTCTTG-3’) and cloned into the pENTR/D-TOPO vector (ThermoFisher) generating the entry vector pENTR p53(R273C ...
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bioRxiv - Cell Biology 2021Quote: ... dried peptide sample was resuspended in 200 mM HEPES (pH 8) and incubated for 1 h at room temperature with one of the TMT10-plex reagents (ThermoFisher) solubilized in 100% anhydrous ACN ...
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bioRxiv - Immunology 2022Quote: ... 8-16 barcoded samples were pooled and loaded on one Ion 530 chip using the Ion Chef Instrument (ThermoFisher Scientific) and sequenced on the Ion S5 System with 550 flows.
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bioRxiv - Cell Biology 2019Quote: ... 5 and 8 were dissociated into single cells with 0.05% Trypsin-EDTA (Invitrogen, 25300062), counted by Countstar (BioTech ...
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bioRxiv - Microbiology 2022Quote: Total RNA from pools of 5 to 8 mosquitoes was isolated with TRIzol (Invitrogen). Small RNAs of 19-33 nucleotides in length were purified from a 15% acrylamide/bisacrylamide (37.5:1) ...
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bioRxiv - Cell Biology 2022Quote: ... we used 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) or FxCycle™ PI/RNase Staining Solution (Thermofisher ...
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bioRxiv - Genomics 2020Quote: ... 7-Aminoactinomycin D (7-AAD) (1:200 dilution, ThermoFisher Scientific #A1310 ...
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bioRxiv - Neuroscience 2022Quote: ... 7-aminoactinomycin D (7-AAD, Thermo Fisher, A 1310) was added 1:50 as a cell death marker.
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bioRxiv - Biochemistry 2019Quote: ... 4-amino-5-methylamino-2’,7’-difluorofluorescein diacetate (DAF-FM DA; Molecular Probes, Eugene, OR, USA) was used to detect NO production in sorghum genotype stalks in response to inoculation treatment at 7 DPI ...
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bioRxiv - Microbiology 2020Quote: ... samples were desalted using HyperSep Filter Plates with a 5-7 µL bed volume (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: Total RNA was extracted from 30 flies of 5-7 days old by TRIzol reagent (Invitrogen). The genomic template was removed using DNase (Takara) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cultures were passaged every 5 to 7 days with collagenase type IV (Invitrogen; 1 mg/mL). The identities of all parental hESC and hiPSC lines were confirmed by DNA fingerprinting and all cell lines were regularly tested to exclude mycoplasma contaminations using a PCR-based assay ...
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bioRxiv - Immunology 2020Quote: ... then labelled with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluoresceinacetoxymethyl ester (Life Technologies, UK). Neutrophils were then added to wells under normoxia or hypoxia ...
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bioRxiv - Immunology 2021Quote: ... Five minutes prior to analysis 5 μg/ml 7-amino actinomycin D (Life Technologies, CA, USA) was added for exclusion of dead cells ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 minute at 60°C)] on a QuantStudio 5 or QuantStudio 7 qPCR machine (Applied Biosystems). For qPCR assessments ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were split every 5-7 days by incubation with 0.5 μM EDTA (Thermo Fisher Scientific) for 4 min at room temperature and clumps were dissociated into small clumps by pipetting ...
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bioRxiv - Biochemistry 2023Quote: ... we incubated 5-7 μg of BACMID DNA with 4 μL Fugene (Thermo Fisher, Cat# 10362100) in 250 μL of Opti-MEM serum free media for 30 minutes at 23ºC ...
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bioRxiv - Cell Biology 2023Quote: ... COS-7 cells were grown at 37 °C under 5% of CO2 in DMEM Glutamax (Gibco) with 10% bovine fetal serum ...
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bioRxiv - Neuroscience 2023Quote: ... 15-20 pooled day 30 neurospheres and 5-7 pooled day 60 organoids using TRIzol (Invitrogen; Thermo Fisher Scientific Inc. ...
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bioRxiv - Biochemistry 2023Quote: ... 7 mM NaOH) followed by an incubation of 5 hrs with Dynabeads Protein G (Invitrogen, 10004D). Beads were then washed with Wash Buffer (20 mM Tris pH 8.0 ...
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bioRxiv - Immunology 2020Quote: ... Intracellular cytokine staining was performed after incubation for 4-6h with Cell Stimulation cocktail plus Golgi transport inhibitors (Thermo Fisher Scientific) using the BD Cytofix/Cytoperm buffer set (BD Biosciences ...
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bioRxiv - Cell Biology 2020Quote: Dried fractions were resuspended in 8 μL 5% ACN + 5% FA and analyzed on an Orbitrap Fusion with in-line Easy Nano-LC 1000 (Thermo Scientific). Fractions were run as 3 h gradients progressing from 3% ACN + 0.125% FA to 100% ACN + 0.125% FA ...
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bioRxiv - Cell Biology 2022Quote: Dictyostelium discoideum cells were maintained in Hans’ enriched HL-5 media (1.4X HL-5 media with 8% FM, penicillin and streptomycin) at 22°C in petri dishes (Fisher Scientific; FB0875712). A full list of strains used in this study can be found in Appendix Table S3.
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bioRxiv - Developmental Biology 2020Quote: ... Genital ridges from 1-2 litters (7-8 embryos per litter) were dissected out and digested at 37°C for 3 min using TrypLE Express (Thermo Fisher Scientific). For E9.5 and E10.5 stages ...
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bioRxiv - Plant Biology 2019Quote: Total RNA was extracted from Arabidopsis leaf tissues grown under an 8-h photoperiod or cotyledons from etiolated seedlings grown in dark for 7 d by TRIzol (Thermo Fisher Scientific) followed by DNase treatment at 37 °C for 30 min ...
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bioRxiv - Developmental Biology 2019Quote: ... one midgut and 7 µL of Schneider’s medium were placed in each well and topped with a coverslip (Fisher Scientific, #12-545-81). Midguts were imaged using an inverted Zeiss LSM 880 immediately after mounting.
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bioRxiv - Microbiology 2023Quote: ... RNA samples were analyzed by qRT-PCR with Thunderbird Probe One-step qRT-PCR Kit (Toyobo) and QuantStudio 7 Flex Real-time PCR system (Applied Biosystems; Thermo Fisher Scientific). Target RNA levels were normalized to hamster Actb and calculated by the relative standard curve method ...
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bioRxiv - Developmental Biology 2022Quote: ... 7-amino-actinomycin (7-AAD, ThermoFisher, for labeling nonviable cells) stained cells were sorted using a FACS Aria II sorter (BD BioSciences) ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were stained with 7-amino-actinomycin (7-AAD, ThermoFisher) to label nonviable cells and then sorted using a FACS Aria II sorter (BD BioSciences ...
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bioRxiv - Cell Biology 2020Quote: ... Human iPSC lines were maintained in 5% CO2 environment in Essential 8 Medium (Thermo Fisher). For cardiomyocyte differentiation ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... P-gp-overexpressing subline KB-8-5-11 were maintained in DMEM (Thermofisher catalog #11965) with 10% FBS and 1% penicillin/streptomycin at 37°C in 5% CO2 ...
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bioRxiv - Cell Biology 2019Quote: ... for 5 min and separated on a 3-8% tris-acetate gel (ThermoFisher Scientific; EA0375BOX). Following electrophoresis ...
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bioRxiv - Developmental Biology 2023Quote: ... with 5% FBS or at P7-8 in ice-cold Hibernate-A Medium (ThermoFisher, A1247501) with 10% FBS and B-27 Supplement (ThermoFisher ...
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bioRxiv - Biophysics 2023Quote: Cells were seeded onto 5 µg/ml laminin (BioLamina LN511) coated 8-chamber coverglass (Nunc™ Lab-Tek™ II Chambered Coverglass ...
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bioRxiv - Genomics 2024Quote: ... solution (1mg L-Cystine, Sigma; 8 KU of DNase I, Affymetrix; in 5 ml DPBS), retina was incubated at 37C for ∼20min ...
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bioRxiv - Immunology 2021Quote: ... 7-AAD (Invitrogen), Live/dead fixable viability dye (Invitrogen) ...
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bioRxiv - Microbiology 2020Quote: ... bacilliformis shifted to liquid medium at pH 7 using a 5’ RACE System kit (Invitrogen; Carlsbad, CA) according to manufacturer’s protocols and with gene-specific primers (S1 Table) ...
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bioRxiv - Immunology 2021Quote: ... 5/6 weeks and 6/7 weeks post-infection by flow cytometry using counting beads (CountBright, ThermoFisher).
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bioRxiv - Molecular Biology 2020Quote: ... samples were desalted using HyperSep Filter Plates with a 5-7 μL bed volume (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Bone marrow-derived macrophages (BMDM) were differentiated over 5-7 days in DMEM (Gibco, 11960044 or 11885092), 10% FBS ...
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bioRxiv - Genetics 2023Quote: ... and fluorescence was monitored on ABI ViiA 7 and QuantStudio 5 Realtime PCR system (Applied Biosystems, USA). Melting curve analysis was done for each amplicon ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were split every 7 days using 5-minute incubation with 0.5 mM EDTA-PBS (Thermo Fisher) at 37 °C ...