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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 7 8 DIHYDRO 1 3 DIOXOLO 4 5 G ISOQUINOLIN 5 6H ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2021Quote: ... into jaw closer muscles of 5-8 day old mouse pups with 1-2% dilution of Alexa Fluor 488 hydrazide (Life Technologies), under isoflurane anesthesia.
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bioRxiv - Cancer Biology 2024Quote: ... Proteins were separated by NuPAGE 3-8% or 4-12 % Tris-Acetate Midi Gel (Invitrogen, Cat# WG1402BX10) and transferred to nitrocellulose membranes (Fisher ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were centrifuged at 500 x g for 5 min (Thermo Scientific ST-16) and washed with 10 mL of PBS twice ...
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bioRxiv - Cancer Biology 2023Quote: ... envelope plasmid pIP/VSV-G (5 µg) (ViraPowerTM Lentiviral Packaging Mix, Thermo Fisher Scientific), 5 µg of lentiviral expression construct shRNA (pLKO.1 Mission shRNA DNA clone ...
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bioRxiv - Cell Biology 2023Quote: ... and 5 g/L (34 mM) L-glutamine (Gibco, 200mM, cat. no. 25030-024) – hereafter called “imaging medium” ...
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bioRxiv - Evolutionary Biology 2023Quote: We performed experimental evolution using SM/5 media [56] (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were centrifuged for 5 min at 300 g and washed with PBS (Gibco) supplemented with 4% FCS and DNase I (LIFE Technologies) ...
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bioRxiv - Cancer Biology 2023Quote: Caspase 3/7 activity was assessed using the CellEvent Caspase-3/7 Green Flow Cytometry Assay Kit (Thermo Scientific, #C10427) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: Epimastigotes and trypomastigotes lysates containing 2 x 107 parasites in 20 µL of SDS-PAGE sample buffer containing 5 mM DTT were boiled for 5 min and loaded onto precast Novex Value 4-12% Tris-Glycine gels (Thermo Fisher). The electrophoresis was performed in 50 mM MOPS-50 mM Tris Base ...
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bioRxiv - Synthetic Biology 2020Quote: ... Cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco #25300-054). The HEK293FT cell line tested negative for mycoplasma with the MycoAlert Mycoplasma Detection Kit (Lonza #LT07-318).
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bioRxiv - Cell Biology 2023Quote: ... KIF18A siRNA used was a 1:1 mixture of the following two Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334) CGUUAACUGCAGACGUAAAtt (Ambion ...
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bioRxiv - Cancer Biology 2023Quote: ... KIF18A siRNAs used were a 1:1 mixture of two the following Silencer or Silencer Select Validated siRNAs (5’ to 3’ sequence): GCUGGAUUUCAUAAAGUGGtt (Ambion, AM51334), GCUUGUUCCAGAAUCGAGAtt (Ambion ...
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bioRxiv - Molecular Biology 2021Quote: ... Cas9 as well as the flanking 5’ and 3’ nuclear localizing sequences and 5’-V5 tag from the GeneArt CRISPR Nuclease Vector (Thermo Fisher Scientific). HEK293-Cas9 cells were maintained in DMEM supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2021Quote: ... oligonucleotides from homologous regions D-ORF5 5′CCGctgagCAAGGTAGCCTCGTCTATTGGAC 3′ and R-ORF7 5′CCGctcgagTTCTTCATCTTCAATATTATGTC3′ were used using the PCR Reagent System Kit (Invitrogen, Life Technologies Inc.). The reaction mixture was prepared with l00 ng of recombinant TnGV DNA ...
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bioRxiv - Cell Biology 2023Quote: ... All drug treatments were performed for 3 – 5 hours prior to imaging with a final concentration of 5 nM of actinomycin D (Gibco, 11805-017) or 1 µM of BMH-21 (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... 1 ml phenol:chloroform 5:1 pH 4.5 (Thermofisher #AM9720) and 0.5 ml glass beads (Sigma #G8772) ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Developmental Biology 2021Quote: Embryos and larvae from 9 hpf to 4 wpf were incubated in 3 µM 5-Ethynyl-2′-deoxyuridine (EdU; ThermoFisher Scientific, cat#: C10639) in FSW for 15-30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Rev: 5’-TCATTGAGACACCATTTGTC-3’ were cloned into pCR™4-TOPO® TA vector using the TOPO-TA cloning kit (Thermo Fisher 450030) and sequence verified.
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bioRxiv - Developmental Biology 2020Quote: ... Genital ridges from 1-2 litters (7-8 embryos per litter) were dissected out and digested at 37°C for 3 min using TrypLE Express (Thermo Fisher Scientific). For E9.5 and E10.5 stages ...
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bioRxiv - Cell Biology 2022Quote: ... 3x105 cells were spun down at 200 g for 5 min and resuspended in 1 mL L-15 media (Gibco) containing 1μL SiR-tubulin (Cytoskeleton Inc. ...
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bioRxiv - Cell Biology 2021Quote: ... 5% CO2 in minimal medium containing 1 part DMEM (4.5 g/L D-Glucose, Pyruvate, L-Glutamine; Invitrogen 11971-025): 1 part DMEM/F12 ...
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bioRxiv - Neuroscience 2021Quote: ... the cell suspension was centrifuged at 200 g for 5 minutes and the resulting pellet was resuspended in 1 mL of DMEM (Gibco) supplemented with 10% FBS and 1% antibiotic-antimycotic (Gibco) ...
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bioRxiv - Neuroscience 2019Quote: ... centrifuged for 5 minutes at 400 × g and resuspended in B27 medium (Neurobasal supplemented with 1% B27 without vitamin A (Gibco), 2 mM L-glutamine and 0.2% penicillin/streptomycin) ...
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bioRxiv - Microbiology 2023Quote: ... The cells were spun down at 1200×g for 5 minutes and washed with 1X PBS+ 1% FBS once before staining with Aqua Live/Dead stain (Invitrogen) at room temperature for 25 minutes ...
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bioRxiv - Immunology 2022Quote: The stained cells were washed with 1X PBS+1% FBS and centrifuged at 1200×g for 5 min twice and stained with the 1:1000 Far Red live/dead staining (Invitrogen) at room temperature for 20 minutes ...
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bioRxiv - Neuroscience 2019Quote: ... and CellEvent caspase-3/7 reagent (ThermoFisher Scientific) were used according to manufacturer protocol ...
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bioRxiv - Immunology 2022Quote: ... and Cell Event Caspase-3/7 Green (Invitrogen) were loaded at the final concentration of 5 µM and 7 µM respectively along with the cells ...
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bioRxiv - Cancer Biology 2020Quote: ... Caspase-3 and −7 Cell Imaging Kit (Invitrogen), and Propidium iodide (PI ...
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bioRxiv - Systems Biology 2024Quote: ... CellEventTM Caspase 3/7 detection reagent (Invitrogen C10423), and SYTOXTM AAdvanced Dead Cell Stain (Invitrogen S10349) ...
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bioRxiv - Immunology 2024Quote: ... 5×10-5 M 2-mercaptoethanol (Gibco) and 50µg/mL Gentamicin (Lonza ...
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bioRxiv - Microbiology 2021Quote: ... and customized si-KIF4 3′ UTR targeting endogenous KIF4 mRNA 3’-UTR region (5′-GGAAUGAGGUUGUGAUCUUTT-3′) were purchased from Thermo Fisher Scientific.
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bioRxiv - Bioengineering 2023Quote: Passage 3 and passage 4 cells from 8 donors (4 male and 4 female) were expanded in T175 flasks (Thermo Fisher Scientific, Hampton, New Hampshire USA) and were cultured until passage 5 (p3-5 and p4-5 ...
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bioRxiv - Microbiology 2020Quote: ... Pre-cleared lysates were further incubated at 4°C overnight with the indicated antibodies (1 to 3 μl) and protein A agarose or protein G Dynabeads (Thermo Fisher Scientific). Immunoprecipitates were washed three times with RIPA buffer (LSB ...
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bioRxiv - Neuroscience 2021Quote: ... brains were then incubated in secondary antibodies (diluted in 5% serum in PBT at 4°C for 2–4 days): Alexa 488 anti-Chicken IgY (Invitrogen A11039; 1:400); Atto 647N anti-mouse IgG (Rockland 610-156-121 ...
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Precision genetic cellular models identify therapies protective against endoplasmic reticulum stressbioRxiv - Neuroscience 2020Quote: ... One µL of cDNA was used in each qPCR reaction on a QuantStudio 5 (ThermoFisher Scientific) using SYBR Green PCR Master Mix (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... and 4’,6-diamidino-2-phenylindole at 5 µg/mL (DAPI; ThermoFisher) in TBS 1% BSA ...
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bioRxiv - Molecular Biology 2020Quote: ... siRNA (4): s35234 and siRNA (5): s35235 (Silencer Select, ThermoFisher Scientific, USA); EMC2 s18670 and EMC5 s41131 (Silencer Select ...
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bioRxiv - Developmental Biology 2020Quote: ... containing 4’,6-diamidino-2-phenylindole (DAPI, 5 µg/ml, Life Technologies).
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bioRxiv - Microbiology 2019Quote: ... The membrane was stained with 5 μg/ml FM 4-64FX (Invitrogen) fluorescent dye while the nucleoid was stained with 2 μg/ml DAPI (Millipore Sigma ...
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bioRxiv - Systems Biology 2019Quote: ... the reaction was quenched with 4 μL 5% hydroxylamine (Thermo Scientific, 90115) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... 2.5-5 µg was loaded onto a NuPAGE 4-12% gel (ThermoFisher) and run at 100V for 2-3 h ...
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bioRxiv - Evolutionary Biology 2022Quote: ... and passaged every 4-5 days using StemPro Accutase (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... 5 ng/ml IL-4 (Thermo Fisher Scientific, Cat#14-8041-62) and 1 ng/ml TGF-β1 (Cell Signaling Technology ...
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bioRxiv - Bioengineering 2023Quote: Samples were loaded with 5 μM Fluo-4 AM (ThermoFisher Scientific, Germany) in CM for 30 min in a cell culture incubator (37 °C and humidified 5% CO2) ...
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Upregulated GIRK2 counteracts ethanol-induced changes in excitability & respiration in human neuronsbioRxiv - Neuroscience 2024Quote: ... After 4-5 minutes 30 µM SCH-23390 (Fisher Scientific Cat#: 092550) in ACSF was applied for 1 minute ...
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bioRxiv - Molecular Biology 2023Quote: ... washed twice in PBS and after centrifugation at 250 x g for 5 min at 4°C resuspended in cell lysing buffer (Cell Signaling Cat# 9803, containing protease inhibitor, Thermo Fisher Scientific, Cat# 78415). Cell lysates were incubated on ice for 45 min with occasional mixing ...
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bioRxiv - Immunology 2020Quote: ... Intracellular cytokine staining was performed after incubation for 4-6h with Cell Stimulation cocktail plus Golgi transport inhibitors (Thermo Fisher Scientific) using the BD Cytofix/Cytoperm buffer set (BD Biosciences ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed 4 × 5 min with PBS before incubating with secondary antibodies (1:1000 Alexa fluorophores, ThermoFisher) in blocking solution for 1 hr at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... centrifuged at 500xg for 5 min at 4°C and resuspended in 1% BSA in PBS (AM2616, Invitrogen) containing 200 U/μl RNase inhibitor (3335402001 ...