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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 7 8 9 10 TETRAHYDRO 6H AZEPINO 2 1 B QUINAZOLIN 12 YLIDENEAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: We previously cloned the genes for TEM and CTX-M-9 β-lactamase into pET24-b plasmids (Life Technologies) for inducible protein expression under the T7 promoter.[21 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Isolated B cells were activated at 1 x 106 cells mL-1 for 2 days in IMDM medium (ThermoFisher) with 10% fetal bovine serum ...
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bioRxiv - Microbiology 2021Quote: ... 800 µl of isolation medium (DMEM, supplemented with 2% FBS, 1% penicillin-streptomycin and 1% amphotericin B, ThermoFisher Scientific) was added to each well ...
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bioRxiv - Neuroscience 2020Quote: ... Neurons were maintained in Neurobasal A medium supplemented with 2% B-27 and 1% GlutaMAX (Gibco) in an incubator at 37°C and 5% CO2 ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in 2-(N-(7-nitrobenz-2-oxa-1,3-diaxol-4-yl) amino)-2-deoxyglucose (2-NBDG) (Invitrogen) (20 µM ...
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bioRxiv - Physiology 2021Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in saline solution at 5 mg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (100 μM; ThermoFisher Scientific) with Hoechst (1 μg/mL ...
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bioRxiv - Physiology 2019Quote: ... 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher Scientific, USA) was dissolved in PBS at 5 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Glucose uptake assay was performed by incubating cells with 50 µM 2-NBDG (2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose) (Invitrogen) for 90 min at 37°C prior to cell staining ...
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bioRxiv - Immunology 2022Quote: ... cells were resuspended in glucose-free media containing 150 μM 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG Thermofisher) and incubated for 45 minutes at 37°C.
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bioRxiv - Microbiology 2019Quote: ... were maintained in HMI-9 (HMI-9 (Gibco), 20 % v/v FCS (Gibco) ...
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bioRxiv - Immunology 2022Quote: ... was used to dissociate cells before screening or sorting with anti-CD58 (TS2/9, Invitrogen 12-0578-42) or anti-ICAM1 (HA58 ...
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bioRxiv - Neuroscience 2021Quote: ... medium was changed to DMEM/F-12 supplemented with 1× N-2 supplement (Life Technologies), 2 µg/mL heparin ...
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bioRxiv - Microbiology 2021Quote: ... 2 μL of 10 mM NBD-(linezolid-7-nitrobenz-2-oxa-1,3-diazol-4-yl)-amino-D-alanine (NADA) (Thermo Fisher) was added to each tube and incubated at 37° C ...
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bioRxiv - Immunology 2021Quote: ... Transduction efficiency was determined between days 8-10 of culture using a polyclonal goat anti-human IgG Fc PE (ThermoFisher Scientific, #12-4998-82) and the synaptic transfer to BSLB addressed immediately.
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bioRxiv - Genomics 2020Quote: ... 10 mL B-27 Minus Insulin (A1895601, ThermoFisher Scientific), 5 mL Glutamax (35050-061 ...
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bioRxiv - Cancer Biology 2019Quote: ... antibiotics and 10 μg/ml hygromycin B (Thermo Fisher). All cell lines were obtained from American Type Culture Collection (ATCC ...
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bioRxiv - Developmental Biology 2023Quote: ... with 10% FBS and B-27 Supplement (ThermoFisher, 17504044). The same media were used during flow cytometry sorting ...
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bioRxiv - Immunology 2019Quote: ... and 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) were from Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA, Molecular Probes, Eugene, OR, USA). Conidia were incubated in an optimized minimal medium for 20 h at 25°C ...
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bioRxiv - Cancer Biology 2020Quote: 4-Amino-5-Methylamino-2’,7’-Difluorofluorescein Diacetate (DAF) (ThermoFisher) was used to measure and spatially resolve nitric oxide (NO ...
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bioRxiv - Immunology 2021Quote: ... 5 μM of 2′,7′-Dichlorofluorescin diacetate (DCFH-DA, Invitrogen) probe was added to each neutrophil subtype and incubated in the dark for 15 min ...
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bioRxiv - Neuroscience 2019Quote: ... 2’,7’-Dichlorodihydrofluorescein diacetate (Thermo fisher, Shanghai, China, cat# 2938), JC-1 probe solution (Sigma ...
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bioRxiv - Cancer Biology 2023Quote: ... the cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Invitrogen, # D399), was used to estimate Reactive Oxygen Species (ROS ...
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bioRxiv - Bioengineering 2020Quote: ... 8 μL of 10 mM dNTPs (Fisher Scientific), 5 μL of 10X E Mix and 5 μL of control primer Mix (30 bp ...
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bioRxiv - Molecular Biology 2020Quote: ... peptides were trapped at 3 μl/min on a 10 mm × 75 μm ID Acclaim Pepmap trap at 2% buffer B (buffer A: 0.1% formic acid (Fisher Scientific), buffer B ...
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bioRxiv - Cell Biology 2023Quote: ... Proteins were analyzed on 4-12% or 8-16% SDS-PAGE gradient gels (Invitrogen) and transferred to nitrocellulose membrane (BioRad) ...
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bioRxiv - Microbiology 2023Quote: ... HCT-8 cells were plated on 12-mm-diameter glass coverslip (Thermo Fisher Scientific) in 24-well tissue culture plates and incubated until confluent ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Cell Biology 2022Quote: ... 9 μl of carboxyl latex bead (4% w/v, 2 μm; Invitrogen, C37278), and 2 μl of Concanavalin A (1 mg/ml ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... Matrix Metalloprotease 9 (MMP9, Thermo Fisher Scientific, 1:1000), β-actin (Santa Cruz ...
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bioRxiv - Cell Biology 2023Quote: ... tracheas from adult (8- to 12-weeks old) mice were dissected and cleaned in ice-cold Ham’s F-12 medium (Life Technologies, #31765-092) with 1% penicillin/streptomycin (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Each oligo was diluted to 100 μM in nuclease-free 10 mM Tris pH 8 (diluted from Ambion 1 M Tris pH 8; Invitrogen; cat. no. AM9856). The rRNA depletion mix was generated by combining equal volumes of each 100 μM oligonucleotide stock.
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bioRxiv - Cell Biology 2023Quote: ... Cells were grown in DMEM / F-12 (1:1) + 2.5 mM L-glutamin + 10% FBS (Thermofisher Scientific, USA) + 1% penicillin / streptomycin (Thermofisher Scientific ...
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bioRxiv - Evolutionary Biology 2023Quote: ... The cells were passaged at a proportion of 1:8-1:10 with trypsin (Life Technologies, #25200-056) every 3 days or when they reached 80% confluence.
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bioRxiv - Immunology 2021Quote: ... Glucose uptake was measured by the uptake of glucose analogue 2-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG, Life Technologies). Cell surface and intracellular cytokine stainings of splenocytes and blood lymphocytes were performed as described (42) ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated in glucose-free media containing 5 μg/ml 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Thermo Fisher) and 2.5% FBS at 37 °C ...
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bioRxiv - Physiology 2020Quote: ... 1 mM fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Life Technologies) was diluted in Live Cell Imaging Solution (Invitrogen ...
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bioRxiv - Cell Biology 2022Quote: Cellular glucose uptake was measured by culturing cells in glucose free DMEM for 2 hours before adding 100µM of the fluorescent glucose analogue 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl) Amino)-2-Deoxyglucose (2-NBDG; ThermoFisher, N13195). The analogue was incubated with MCF7s for 30 minutes and MSCs for 60 minutes at 37°C ...
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bioRxiv - Neuroscience 2020Quote: ... and equally distributed in wells containing growth medium (DMEM-F12-glutamax [Gibco], 40 μg/mL insulin [Sigma], 1/200 B-27 supplement [Gibco], 1/100 N-2 supplement [Gibco] ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 2% B-27 (Thermo Fisher Scientific, 17504-044), 2 mM L-glutamine and 1% penicillin– streptomycin ...
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bioRxiv - Neuroscience 2020Quote: ... and 2 % B-27™ Supplement (17504001, Thermo Fisher Scientific). Cultured neurons were maintained in a 5% CO2 incubator at 37°C before experiments were performed.
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bioRxiv - Neuroscience 2020Quote: ... and and 2% Gibco™ B-27™ supplement (ThermoFisher) (subsequently called NB5 media) ...
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bioRxiv - Cell Biology 2021Quote: ... fresh YPD contained phloxine B (2 μg/mL, Fisher Scientific). For imaging in FITC/GFP channels ...