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Citations for Thermo Fisher :
1 - 50 of 9252 citations for 7 12 Dioxa spiro 5.6 dodec 9 ene since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Monocytes adhered to glass coverslips in 12-well plates (1.5×106 cells/well) and were subsequently cultured for 7–9 days in RPMI (Gibco) with 10% FBS (Wisent ...
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bioRxiv - Cell Biology 2021Quote: ... a 7-9% agarose (Invitrogen, cat no. 16500500) pad made from the imaging media was gently placed over the top of the worms ...
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bioRxiv - Systems Biology 2020Quote: ... 5.6 μL BSA (B14, Thermo Scientific), and 27.9 μL T4 ligase ...
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bioRxiv - Immunology 2021Quote: ... 5.6 μl P3000 reagent was added (Thermofisher). Simultaneously ...
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bioRxiv - Cell Biology 2019Quote: SCC-9 cells were maintained in DMEM/F-12 (Gibco) supplemented with 10% FBS (Hyclone) ...
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bioRxiv - Microbiology 2022Quote: ... 15% formamide (Cat: 75-12-7; Acros Organics), 0.2 mg/mL UltraPure BSA (Cat ...
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bioRxiv - Systems Biology 2021Quote: ... and D-glucose (5.6 mM, Thermo Fisher, Waltham, MA), and the culture was allowed to equilibrate for 40 min ...
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bioRxiv - Genetics 2023Quote: ... A clean glass slide (Fisher Scientific, 12-544-7) was dipped into freshly made PFA solution (1% paraformaldehyde adjusted to pH 9.2 using 1.25 M sodium borate ...
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bioRxiv - Cell Biology 2020Quote: ... 5.6 mM D-Glucose and MEM amino acids solution (ThermoFisher 11130051 ...
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bioRxiv - Cancer Biology 2022Quote: ... on microscopy plates (Cat. #12-544-7, Thermo Fisher Scientific), and sealed with nail polish (Cat ...
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bioRxiv - Cancer Biology 2021Quote: ... and mPRs (PAQR5, 6, 7, 8, 9) using Power SYBR Green Master Mix with ViiA 7 Real-Time PCR System (Applied Biosystems). RT-qPCR plates with various cell-lines were prepared using an epMotion 5075 automated liquid handling system (Eppendorf) ...
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bioRxiv - Neuroscience 2020Quote: ... 1.2 μg of all others) at 7–9 DIV with Lipofectamine 2000 (Life Technologies), and all live imaging was performed between 12–16 h after transfection ...
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bioRxiv - Molecular Biology 2019Quote: ... either 50 µl (Figure 2A-2C, 7 and 8) or 25 µl (Figure 2D, 2E, 3-7 and 9) magnetic beads (Dynabeads protein G, Life Technologies #10004D) was used ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were mounted on microscope slides (Fisher Scientific 12-544-7) with EverBrite Mounting Medium with DAPI (Biotium 23002 ...
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bioRxiv - Molecular Biology 2023Quote: ... 5.6 ug lentiviral vector and 14.4 ug ViraPower Lentiviral Packaging Mix (Invitrogen) were mixed and transfected using Lipofectamine 3000 (Invitrogen ...
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Induction of Dopaminergic Neurons for Neuronal Subtype-Specific Modeling of Psychiatric Disease RiskbioRxiv - Neuroscience 2021Quote: ... Coverslips were carefully transferred to glass slides (Fisher Scientific, #12-544-7) and fixated using AquaPolymount (Polysciences Inc. ...
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bioRxiv - Immunology 2023Quote: ... EndoC-βH1 cells were cultured in DMEM containing 5.6 mmol/L glucose (Gibco, Thermo Fisher Scientific ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The cell suspension was spread on a slide (Fisher Scientific, 12-544-7) coated in 1% paraformaldehyde with 0.15% Triton-X-100 in H2O pH9.2 and air-dried overnight in a humid chamber ...
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bioRxiv - Neuroscience 2021Quote: ... the neurons were transfected after 7-12 DIV using the Lipofectamine 2000 (#11668027, Invitrogen) reagent as previously described 40 ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Cell Biology 2020Quote: Bone marrow cells were isolated and cultured for 7±9 d in RPMI-1640 medium (Gibco, ThermoFisher, Waltham, MA) supplemented with 10% low endotoxin defined FBS (Hyclone ...
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bioRxiv - Genetics 2021Quote: ... was added (500 U per tube) together with 5.6 μl 10X Buffer B (ER0211, Invitrogen) for 18 hours digestion at 37 °C with shaking (700 RPM) ...
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bioRxiv - Genetics 2023Quote: ... Fixed cell suspension was dropped onto a clean glass slide (Fisher Scientific, 12-544-7) from a height of 2-3 feet height using a glass Pasteur pipette in a room with >40% humidity ...
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bioRxiv - Microbiology 2019Quote: ... were maintained in HMI-9 (HMI-9 (Gibco), 20 % v/v FCS (Gibco) ...
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bioRxiv - Genetics 2022Quote: ... Sperm spreads were obtained by dissecting 10-12 male worms in 7 microliters of egg buffer (Edgar 1995) on ColorFrost Plus slides (Fisher Scientific, 12-550) that were additionally coated with poly-L-lysine (Sigma Aldrich ...
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bioRxiv - Immunology 2022Quote: ... was used to dissociate cells before screening or sorting with anti-CD58 (TS2/9, Invitrogen 12-0578-42) or anti-ICAM1 (HA58 ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: ... Monocytes were then differentiated into macrophages by 7-9 days of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Bioengineering 2023Quote: ... Monocytes were then differentiated into macrophages by 7–9 d of culture in IMDM + 10% AB human serum (Life Technologies).
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bioRxiv - Biochemistry 2023Quote: ... Cell proliferation was assessed at different time points (3, 5, 7 and 9 days) using AlamarBlue Cell Viability Reagent (Invitrogen) per manufacturer’s instructions ...
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bioRxiv - Cell Biology 2019Quote: ... Canada) were cultured in regular DMEM medium containing 5.6 mM glucose (Cat# 12320032, Thermo Fisher Scientific) supplemented with 15% horse serum (Cat# 26050088 ...
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bioRxiv - Immunology 2019Quote: ... cells were treated with TURBO™ DNase (12 units/10^7 cells, cat# AM2239, ThermoFisher Scientific) for 1 hour at 37°C ...
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bioRxiv - Biochemistry 2023Quote: ... Hippocampi of male and female E17 mouse brains from a single litter (7-9 embryos) were dissected in HBSS (ThermoFisher Scientific) supplemented with 1% L-glutamine ...
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bioRxiv - Developmental Biology 2023Quote: ... Tissues from 10 – 15 dpf zebrafish were mounted on Premium microscope slides (Fisher Scientific, 12-544-7) with Premium cover glass (Fisher Scientific,12-548-B ...
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bioRxiv - Developmental Biology 2021Quote: Protein was extracted from approximately 50mg of placental Jz tissue (n = 7 per genotype/sex, across 9 litters) using RIPA buffer (Thermo Scientific, US) containing cOmplete Mini EDTA-free protease inhibitor cocktail mix (Roche ...
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bioRxiv - Biochemistry 2019Quote: The N30 Random DNA pool and cDNA from rounds 7-to-9 were cloned into TOPO™ TA Cloning™ Kit (Invitrogen) following manufacturer’s specifications ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Cancer Biology 2020Quote: ... BT-12 and BT-13[7] were cultured in Dulbecco’s modified Eagle’s medium with Nutrient Mixture F-12 (DMEM/F12, Gibco) supplemented with 2 mM L-glutamine ...
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bioRxiv - Bioengineering 2022Quote: ... COS-7 cells were plated onto Nunc Lab-Tek II Chambered Coverglass (Thermo Fisher Scientific, 12-565-335) to achieve ~70% confluence before transfection.
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bioRxiv - Molecular Biology 2022Quote: ... Fractions of 7 to 12 representing the translation level were taken for RNA isolation with TRIzol reagent (Ambion). To perform RNA-seq experiment ...
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bioRxiv - Developmental Biology 2022Quote: ... we found that poly-lysine treating the coverslips was not necessary for immobilization of the worms in most cases as long as the liquid under the agarose pad is minimal.) 7-9% agarose pads (Invitrogen, cat no. 16500500) were gently placed over the top of the worms and excess liquid was wicked away using Whatman paper ...
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bioRxiv - Microbiology 2021Quote: ... Rorγt (AFKJS-9, Thermofisher), IL-10 (JES5-16E3 ...
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bioRxiv - Microbiology 2022Quote: ... containing 9% FBS (Gibco) and 100 µg/ml Pen/Strep (ThermoFisher) ...
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bioRxiv - Developmental Biology 2023Quote: ... 9 mM DTT (Invitrogen), 3.6 U/µL RnaseOUT (Invitrogen) ...
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bioRxiv - Genomics 2023Quote: ... 9 mM MgCl2 (Invitrogen), 1 μM Template-Switching Oligo (TSO ...