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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 7α 12α Dihydroxy 5β cholestan 3 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... One wash included Hoechst 33342 (1:10,000; H-3570; Molecular Probes) to stain nuclei ...
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bioRxiv - Physiology 2021Quote: ... and analyzed with SuperScript III Platinum SYBR Green One-Step (Invitrogen). As previously described41,42 ...
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bioRxiv - Physiology 2021Quote: ... and quantity was determined using a NanoDrop One spectrophotometer (Thermo Scientific).
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... One testis was cut half and homogenized with Trizol (Thermo Fisher) for RNA isolation and snap-frozen for protein extraction ...
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bioRxiv - Plant Biology 2021Quote: ... in a Step-One Plus Real-Time PCR system (Applied Biosystems). The expression levels were normalized against a ubiquitin reference gene ...
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bioRxiv - Microbiology 2020Quote: ... We transformed One Shot™ BL21(DE3) Chemically Competent (Thermo Scientific) E ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by addition of one volume of 70% ethanol (Fisher Scientific) and mixed again by pipetting ...
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bioRxiv - Cell Biology 2020Quote: ... One microgram of RNA was reverse transcribed using SuperScript III (Invitrogen) and quantitative PCR were assayed using Mus musculus primers against Vegfa (Mm00437306_m1 ...
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bioRxiv - Developmental Biology 2022Quote: Total RNA from one worm was extracted with TRIzol™ (Invitrogen) using the standard protocol ...
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bioRxiv - Pathology 2022Quote: hPCLS were infected for one hour in 100 µL MEM (Gibco) in 24-well plates at 37°C with human rhinovirus 16 (Virapur ...
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bioRxiv - Biophysics 2022Quote: ... cDNA concentration was checked via NanoDrop (Thermo Scientific, ND-ONE-W), and then aliquoted and stored at −20°C until used for PCR ...
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bioRxiv - Biochemistry 2022Quote: ... Final protein concentration was measured using NanoDrop One (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2022Quote: ... which was grown in One Shot PIR1 cells (Thermo Fisher Scientific). Plasmids were purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2022Quote: ... DNA was quantified in a Nanodrop One spectrophotometer (Thermo Fisher Scientific) and by fluorometric quantitation using a PicoGreen device (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... Protein concentration was determined with NanoDrop One spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2022Quote: One million fibroblast cells were harvested using TrypLE Express (Gibco, 12604021) and electroporated with non-integrating episomal vectors using a Neon transfection system (Invitrogen ...
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bioRxiv - Neuroscience 2021Quote: ... RNA concentrations were measured using a NanoDrop One spectrophotometer (ThermoFisher Scientific). 2μg of RNA was used to produce cDNA using First Strand cDNA Synthesis kit (Roche ...
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bioRxiv - Biochemistry 2021Quote: ... and culture of one representative clone in BMGY/BMMY (Invitrogen, USA) resulted in the secretion into the supernatant of an RBD variant tagged C-terminally with C-myc and His6 sequences ...
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bioRxiv - Microbiology 2021Quote: ... This was followed by one wash with nuclease free water (Ambion). Cover slips were then soaked using lint free paper and mounted with Prolong Diamond antifade mountant with DAPI (Invitrogen) ...
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bioRxiv - Biophysics 2021Quote: ... The concentration of d-ssDNA was measured with NanoDrop One (ThermoFisher).
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bioRxiv - Microbiology 2021Quote: FAS was purified from PichiaPink™ yeast strain one (Invitrogen, USA), which was grown according to the manufacturer’s instructions.
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bioRxiv - Genetics 2021Quote: ... and the Step-One-Plus Real-Time PCR System (Applied Biosystems). All reactions were performed in technical triplicates and the results represent biological triplicates ...
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bioRxiv - Genomics 2022Quote: ... One drop of Hoechst 33342 Ready Flow™ Reagent (Invitrogen, R37165) was mixed with 2 ml of PBS and incubated with the cells for 2 min at room temperature ...
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bioRxiv - Cell Biology 2022Quote: ... method and RNA concentration was determined using NanoDrop One (Thermo Scientific). Reverse transcription was performed with 500-1000ng of DNase-treated (Promega ...
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bioRxiv - Biochemistry 2022Quote: ... and RNA concentration and purity was assessed (Thermo Fisher, NanoDrop One).
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bioRxiv - Neuroscience 2022Quote: ... RNA yield was quantified with a nanodrop (Thermo Scientific, Nanodrop One), RNA aliquoted ...
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bioRxiv - Genomics 2022Quote: ... tested for purity using the NanoDrop™ One spectrophotometer (ThermoFisher Scientific), and quantified fluorometrically using the Qubit dsDNA High sensitivity kit ...
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bioRxiv - Genetics 2019Quote: ... on a Step-One Plus Real time PCR system (Applied Biosystems). All reactions were run as follows ...
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bioRxiv - Microbiology 2019Quote: ... One step RT-PCR kit (Thermo Fisher Scientific, Waltham, MA, USA) on the Applied Biosystems 7500 FAST following the previously described protocols.
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bioRxiv - Genetics 2019Quote: ... for one hour and subsequently in 10% goat serum (Thermo Fisher) supplemented with 3% Bovine Serum Albumin (BSA ...
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bioRxiv - Microbiology 2020Quote: ... on the Step One Plus Real-Time PCR System (Applied Biosystems).
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bioRxiv - Zoology 2021Quote: ... and the absence of contaminants was confirmed with NanoDrop One (ThermoFisher) with target OD 260/280 and OD 260/230 ratios of 1.8 and 2.0-2.2 ...
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bioRxiv - Molecular Biology 2020Quote: ... and One Shot® TOP10 (Thermo Fisher Scientific, Waltham, MA, USA)) chemically competent Escherichia coli according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2019Quote: ... on a Step One Plus Real-Time PCR System (Applied Biosystems). Primers were designed to be specific for each gene assay and generated by the DNA services core and IDT Technologies ...
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bioRxiv - Molecular Biology 2021Quote: ... Skin biopsies were immediately stored in one milliliter of RNALater (Ambion, Thermo Fisher Scientific Inc. ...
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bioRxiv - Developmental Biology 2021Quote: ... and a Step One Plus Real-time PCR machine (Applied Biosystems). The amounts of mRNA were measured with SYBR Green PCR Master Mix (Ambion) ...
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bioRxiv - Genomics 2021Quote: ... One replicate was treated with blasticidin (Gibco, A1113903, 4 µg/mL), and the other replicate was not treated with blasticidin ...
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bioRxiv - Cancer Biology 2020Quote: ... using the Step One Plus Real-time PCR system (Applied Biosystems). Data represents two independent experiments with three technical replicates per experiment ...
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bioRxiv - Biochemistry 2021Quote: ... which was grown in One Shot PIR1 cells (Thermo Fisher Scientific). Plasmids were purified using the GeneJET plasmid miniprep kit (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Real-Time PCR System using Step One software v2.0 (Applied Biosystems). All qRT-PCR reactions were performed in duplicate ...
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bioRxiv - Cancer Biology 2021Quote: ... One-Step™ Turbo TMB-ELISA Substrate Solution (Thermo Fisher Scientific) was added to the wells for color development ...
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bioRxiv - Immunology 2021Quote: ... using three Dynabeads per one Treg cell (Thermo Fisher Scientific, USA) for activation ...
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bioRxiv - Cell Biology 2021Quote: ... RNA concentrations and purity were assessed using a NanoDrop One (ThermoFisher). Samples with A260/A280 ratio < 2 were subjected to further DNAse treatment using DNAse I ...
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bioRxiv - Developmental Biology 2022Quote: ... One volume of 4 °C DMEM (Thermo Fisher Scientific, Cat# 11960044) with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Immunology 2022Quote: ... supplemented with 10% One Shot dialyzed FBS (Thermo Fisher A33820-01), 1mM sodium pyruvate (Gibco 11360-070) ...
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bioRxiv - Molecular Biology 2022Quote: ... One volume of 1X Tris-EDTA buffer (pH 7.5; Invitrogen, 15567027) and 0.3X room temperature SPRI beads (Beckman Coulter ...
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bioRxiv - Cancer Biology 2022Quote: ... Gene expression was quantified using One-Step qRT-PCR Kits (Invitrogen) in the Applied Biosystems Step One Plus Real-Time PCR System ...
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bioRxiv - Molecular Biology 2022Quote: One microgram of total RNA extracted using the Trizol reagent (Invitrogen) was used for oligo(dT)18-primed cDNA synthesis according to the reverse transcription protocol (TaKaRa) ...
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bioRxiv - Molecular Biology 2022Quote: ... Nucleic acids concentrations were determined with a Nanodrop One (Thermo Scientific), and 50 ng of product was used to prepare TGIRT-seq libraries for high-throughput sequencing.
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bioRxiv - Microbiology 2022Quote: ... using a SuperScript III One-Step RT-PCR Kit (Invitrogen, Germany). Reverse transcription of the viral RNA and amplification of the cDNA was conducted in a LightCycler 480 System (Roche ...