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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 7' BROMO 2' 3' DIHYDRO 1'H SPIRO CYCLOPENTANE 1 4' ISOQUINOLINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Blots were blocked with 5% BSA at room temperature for 1 h and incubated with the following primary antibodies overnight at 4°C: mouse anti-v5 (Thermo Fisher Scientific, Cat. # R96025; 1:2000), mouse anti-Flag M2 (Sigma ...
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Human immunodeficiency virus-1 induces and targets host genomic R-loops for viral genome integrationbioRxiv - Molecular Biology 2024Quote: ... isolated with Protein A Dynabeads (Invitrogen; 4 h at 4°C), washed thrice with RSB+T ...
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bioRxiv - Microbiology 2019Quote: ... pneumoniae TIGR4 was detected using 1:2 optimized rabbit type-4 antiserum and 1:100 anti-Rabbit-Alexa-Fluor-488 (Thermo Fisher). Binding of human FH (Merck Millipore ...
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bioRxiv - Biochemistry 2022Quote: ... pH 7.4/50 mM KOAc/2 mM EDTA, pH 8/1 mM DTT/1 mM PMSF/1x Phosphatase inhibitor cocktail, Thermo Scientific), 0.3 g of glass beads (acid washed ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Developmental Biology 2021Quote: ... samples were washed in block six times for 1 hour and incubated in secondary antibodies conjugated to fluorescent dyes diluted 1:250 in block with added 4’,6-diamidino-2-phenylindole (DAPI; 1:500 dilution of 1 mg/ml stock, Thermo Fisher) for 2 nights at 4°C.
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Cell Biology 2020Quote: ... washed with 2 % FCS/PBS and then re-suspended in 2 % FCS/PBS containing 7-amino actinomycin (7-AAD, Invitrogen) at a concentration of 5 µg/ml ...
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bioRxiv - Immunology 2020Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific). The activated beads were washed three times with 50 mM MES pH 5.0 and added to SARS-CoV-2 S protein which was diluted in 50 mM MES pH 5.0 ...
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bioRxiv - Immunology 2022Quote: ... and 1-Ethyl-3-(3-dimethylaminopropyl) carbodiimide (Thermo Fisher Scientific) and incubated for 30 min on a rotator at room temperature ...
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bioRxiv - Synthetic Biology 2024Quote: ... HEK293FT and HEK293FT-LP cells were subcultured at a 1:5 to 1:10 ratio every 2–3 d using Trypsin-EDTA (Gibco 25300-054). All HEK293FT cell lines generated by engineering either the HEK293FT or HEK293FT-LP parent cell lines were cultured in the same way ...
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bioRxiv - Biochemistry 2021Quote: ... followed by 2 weeks of selection with 3 μg mL−1 of puromycin (Thermo Fisher Scientific). Then ...
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bioRxiv - Developmental Biology 2021Quote: ... anti-phosphohistone 3 (1:100, Invitrogen), anti-phosphoAMPK (1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... TOTO-3 iodide (1/2000, Invitrogen) was added to the secondary antibody solution to label cell nuclei (Invitrogen) ...
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bioRxiv - Genetics 2021Quote: ... 1/3 Neurobasal (Thermo Fisher Scientific), 1x N-2 Supplement (Thermo Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... or ToPro-3 (1:1000, Invitrogen) added to the third wash to stain nuclei ...
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bioRxiv - Microbiology 2023Quote: ... Proteins were migrated for 2-3 h at 120 V in NuPAGE MOPS SDS running buffer (#NP0001, Invitrogen) and transferred onto a PVDF membrane (#1704156 ...
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bioRxiv - Bioengineering 2022Quote: ... 1% N-2 (Thermofisher), 2% B-27 (Thermofisher ...
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bioRxiv - Microbiology 2023Quote: ... 1× N-2 (Gibco), 50 ng/ml of mouse EGF (RnD) ...
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bioRxiv - Cancer Biology 2022Quote: mScarlet-fascin/fascin KD or mScarlet/fascin KD HeLa cells were plated with 2 mM CellEvent Caspase-3/7 Green Detection Reagent (ThermoFisher) in CellCarrier Ultra 384 well plates (PerkinElmer ...
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bioRxiv - Zoology 2022Quote: ... 2.3 × 105 Huh-7 cells were transfected with 2 µg of pSpCas9-BB-2A-GFP-sgPURA using Lipofectamine 2000 (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... 2 units of RNase H (Invitrogen) and 20 units of E ...
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bioRxiv - Neuroscience 2024Quote: ... 2 units of Ribonuclease H (Invitrogen) were added at the end of the procedure to remove any remaining RNA ...
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bioRxiv - Cancer Biology 2021Quote: ... in medium containing active caspase-3/7 detection reagent (ThermoFisher). Chamber slides were mounted on a heated stage within a temperature-controlled chamber maintained at 37°C ...
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bioRxiv - Immunology 2022Quote: ... cells were stained with CellEvent Caspase-3/7 Green (Invitrogen). The cytotoxicity was assessed by flow cytometry as the percentage of Caspase 3/7+ cells in the target cell population ...
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bioRxiv - Immunology 2022Quote: ... stained for apoptosis with CellEvent Caspase-3/7 (Thermo Fisher), incubated for additional 30 min ...
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bioRxiv - Pathology 2022Quote: ... cells were stained for activated caspase 3/7 kit (Invitrogen); 7-amino-actinomycin D (7AAD ...
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bioRxiv - Cancer Biology 2022Quote: ... CellEvent Caspase-3/7 Green Flow Cytometry Kit (Thermo Fisher) was used to measure fraction of apoptotic cells by flow cytometry (BD LSR Fortessa with HTS sampler) ...
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bioRxiv - Cancer Biology 2023Quote: ... Invitrogen CellEvent Caspase-3/7 green detection reagent (C10423, ThermoFisher) was used as stated in manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2024Quote: ... CellEvent Caspase-3/7 Green ReadyProbes™ Reagent (Invitrogen, R37111) was used ...
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bioRxiv - Molecular Biology 2024Quote: ... a Caspase-3/7 assay kit was used (Invitrogen, C10427). Each tube was brought to a volume of 1 ml staining buffer and 1 μl of CellEvent Caspase-3/7 reagent was added ...
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bioRxiv - Molecular Biology 2022Quote: ... 200µl of 2’,7’ – dichlorofluorescindiacetate (H2DCFDA) (Invitrogen, ab113851) was added in each well and the plate was incubated at 37°C in the dark ...
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bioRxiv - Molecular Biology 2023Quote: For 2′,7′-dichlorodihydrofluorescein diacetate (H2DCFDA, Invitrogen(tm) Cat ...
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bioRxiv - Cell Biology 2024Quote: ... 2’,7’-dichlorodihydrofluorescein diacetate (H2DCF-DA, D399, Invitrogen), dihydroethidine (DHE ...
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bioRxiv - Cell Biology 2020Quote: ... before incubating with 4 μg/ml of secondary antibodies for 1 h at room temperature (Alexa fluro 488, 594, 647, Invitrogen). Samples were imaged on an Olympus FV3000 confocal laser scanning microscope.
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Bidirectional neuronal migration coordinates retinal morphogenesis by preventing spatial competitionbioRxiv - Developmental Biology 2021Quote: ... zebrafish embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) while human retinal organoids were incubated at 37°C for 1h in organoid medium supplemented with 10 μm of EdU ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... sections were incubated for 24 h at 4°C with Alexa Fluor488 chicken anti-goat IgG (1/1000, Molecular Probes) or Alexa Fluor488 goat anti-rabbit IgG (1/1000 ...
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bioRxiv - Neuroscience 2021Quote: A stable dispersion of SWCNTs was achieved based on previously published protocols28,43 through tip-sonication for 1 h at 30 % power at 4 °C (Fisher Scientific Model 120 Sonic Dismembrator ...
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bioRxiv - Immunology 2020Quote: ... Twenty-four h post transfection MEFs and HEK293Ts were treated with 4 μg/ml and 1 μg/ml puromycin (Invitrogen) for 48 h ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were fixed and stained for 4 h in 16% PFA solution containing 5 μg mL−1 Hoechst 33258 nuclear stain (Invitrogen). The plates were then imaged using an Operetta High Content Imaging System (PerkinElmer ...
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bioRxiv - Microbiology 2021Quote: ... primary antibody for 16 h at 4 °C and detected with anti-rabbit-Alexa488 (1:200, A11008, Invitrogen, CA, USA) secondary antibodies for 40 min at RT ...
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bioRxiv - Cancer Biology 2021Quote: ... Protein-containing supernatants were pre-cleared for 1 h at 4°C with Dynabead® protein G (Thermo Fisher Scientific). Pre-cleared supernatants were incubated overnight at 4°C with 1:1250 dilution of Rabbit (DAIE ...
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bioRxiv - Neuroscience 2020Quote: ... in 3% NGS and 0.5% Triton-X for 44 h at 4°C and then in goat anti-mouse secondary antibody Alexa 488 (1:500; Invitrogen) in 3% NGS and 0.5% Triton-X for 2 h ...
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bioRxiv - Biochemistry 2020Quote: ... the media was decanted and cells were incubated for 1 h at 37 °C with Fluo-4 Direct dye (Invitrogen) in FLIPR buffer (1× HBSS ...
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bioRxiv - Cell Biology 2021Quote: ... For peptide treatment, cells were washed with PBS and treated with peptides (40 μM, 1–4 h) dissolved in OPTI-MEM (Gibco) acidified with 0.15% (v/v ...
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bioRxiv - Immunology 2022Quote: Bacteria in PBS were first stained for 1 h at 37°C with 4 μM Oregon Green 488-X succinimidyl ester (Invitrogen) followed by 20 μg/ml CypHer5E (Cytiva ...
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bioRxiv - Neuroscience 2022Quote: ... the slices were incubated with the primary antibodies at 4 °C for 48 h (GFP, chicken, abcam, 1:2000; GFAP, rat, Invitrogen, 1:1000 ...
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bioRxiv - Developmental Biology 2022Quote: ... 48 hpf embryos were incubated at 4°C for 1 h in E3 supplemented with 500 μm of EdU (ClickiT-Alexa 488 fluorophore kit, Invitrogen) in 10% DMSO ...
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bioRxiv - Microbiology 2023Quote: ... for 16 h at 4°C and with a secondary fluorescent antibody (Alexa-488 anti-sheep diluted 1:50,000; Thermo Fisher) for 1 h at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... at a final concentration 2.5 μg/mL for 1 h at 4 °C followed by Concanavalin A conjugated with Alexa Fluor 350 (Thermo Scientific) at final concentration 0.5 mg/mL for 3 h at 4 °C ...