Labshake search
Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 6H dibenz C E 1 2 oxaphosphorin 6 oxide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Plant Biology 2021Quote: ... two SlP4H3 OEX lines (#1, #2) and three SlP4H3 RNAi lines (#1, #6 and #7) by using the TRI reagent (Thermo Fisher Scientific, Waltham, MA, USA). Reverse transcription of approximately 1 μg of total RNA was performed with Superscript II® Reverse Transcriptase (Thermo Fisher Scientific ...
-
bioRxiv - Immunology 2022Quote: ... Nitric oxide was measured using the Griess reagent system from Invitrogen (Cat #G7921).
-
bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
-
bioRxiv - Neuroscience 2021Quote: ... Cell nuclei were stained for 30 min with 4’,6-diamidino-2-fenylindool (DAPI, 1:1000 in PBS, Thermo Fisher Scientific). After immunolabelling ...
-
bioRxiv - Plant Biology 2021Quote: ... Tissues were counterstained with DAPI (4′,6-diamidino-2-phenylindole, 1 μg/ml in PBS) and mounted with ProLong Gold (ThermoFisher Scientific) using #1.5 coverslips (thickness ...
-
bioRxiv - Microbiology 2020Quote: ... Light organs were then counterstained overnight with a 1:750 dilution of 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) in 5x SSC-Tween before mounting on slides with Vectashield (Vector Laboratories ...
-
bioRxiv - Neuroscience 2022Quote: ... Cells were washed 3x with 1x PBS (5 minutes per wash) prior to 4’,6-diamidino-2-phenylindole (DAPI; 1:3000; Thermo Fisher) incubation for 5 minutes at room temperature to stain nuclei ...
-
bioRxiv - Evolutionary Biology 2022Quote: ... All slides were then rinsed three times with PBS-T and twice with 1× PBS and coated with 4’,6-diamidino-2-phenylindole (DAPI) containing the antifade reagent ProlongGold (Life Technologies). Images were acquired using an Olympus BX63 automated fluorescence microscope equipped with an Olympus DP74 digital camera and evaluated with cellSens Dimension software (Olympus).
-
bioRxiv - Biochemistry 2020Quote: ... at 37°C for 20 min and stained with DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate, 1:1000 in PBS, pH 7.4, Thermo Fisher Scientific) before imaging on a Nikon Eclipse Ti-E inverted microscope with a 100X lens (Apochromat 1.49 Oil 0.13-0.20 DIC N2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... cells were transfected using 1-2 µg of pMX-GFP or pMX-53BP1 vectors and 6 µL Lipofectamine 2000 (Thermo Scientific). The media was changed after 3 hours of incubation ...
-
bioRxiv - Molecular Biology 2022Quote: ... The gonads were washed 3 times and then stained with 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI) before mounting with antifade solution (Invitrogen, P36980) on poly-L-lysine coated glass slides (Sigma Merck ...
-
bioRxiv - Bioengineering 2022Quote: ... The cell concentration was diluted 1:6 and cultured in 2 mL of Essential 8 (E8) - Flex Medium Kit (ThermoFisher Scientific) supplemented with 1% Penicillin Streptomycin (GIBCO ...
-
bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
-
bioRxiv - Bioengineering 2023Quote: ... to each well we add 200 μL of 1 μg/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Cat. No. D3571) solution in DI water ...
-
bioRxiv - Systems Biology 2023Quote: ... the cell nuclei were stained with 4′,6-diamidino-2-phenylindole (DAPI, 1 μg/mL, Thermo Fisher Scientific, Waltham, MA, USA) in PBS for 15 minutes at room temperature ...
-
bioRxiv - Bioengineering 2023Quote: ... followed by washing and addition of secondary antibodies and 4′,6-diamidino-2-phenylindole (DAPI) (1:1000, Thermo Fisher Scientific, 62248) for 30 mins ...
-
bioRxiv - Developmental Biology 2024Quote: Total protein was extracted from 1-2 wells of a 6-well plate using RIPA lysis buffer with protease inhibitor (ThermoFisher Scientific). Protein concentration was measured using Pierce BCA kit (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2023Quote: ... 0.7 mL Buffer 2 (1 M sorbitol, 50 mM MES, pH 6, 5 mM MgCl2) plus protease inhibitors (Thermo Scientific A32955) was added ...
-
bioRxiv - Microbiology 2023Quote: ... sections were counterstained with 1 µg/ml of 4’,6-diamidino-2-phenylindole (DAPI, #D1306, Thermo Fisher Scientific, Waltham, MA, USA) then ...
-
bioRxiv - Pathology 2019Quote: ... and visualized on 1% agarose e-gel Power Snap (ThermoFisher Scientific, Waltham, MA).
-
bioRxiv - Microbiology 2020Quote: ... The PCR products were analyzed on an E-Gel 1% agarose (Thermofisher Scientific). The sequences of Luc/GFP primer set used to amplify the sg mRNA were ...
-
bioRxiv - Biochemistry 2019Quote: ... Filtrate was added onto DNA Polymerase 1 (PolI) (E. coli source, Fisher Scientific), 0.25U/uL reaction ...
-
bioRxiv - Developmental Biology 2021Quote: ... and rat anti-E-cadherin (1:1000, Thermo Fisher, Cat. No. 13-1900). Sections were washed in PBS with 0.1% Tween-20 four times for 5 minutes at room temperature and incubated in secondary antibodies and DAPI (1:500 dilution of 1 mg/ml stock ...
-
bioRxiv - Microbiology 2023Quote: ... MHC II (I-A/I-E) (SuperBright645, Thermo Fisher, 1:200,M5/114.15.2), MHC I (H-2Kb/Db ...
-
bioRxiv - Genomics 2022Quote: ... DNA quality was checked on a 1% agarose gel (E-gel EX Invitrogen). 500ng DNA was used from each sample for further analysis ...
-
bioRxiv - Microbiology 2023Quote: ... Tissues were maintained in E-medium (3:1 ratio of DMEM:F12 (Thermofisher, 21765029) medium supplemented with 10 % FBS and 1 % PS ...
-
bioRxiv - Microbiology 2019Quote: ... The resulting ~390 bp amplicon from the pool was then gel-purified utilizing a 2% E-gel (Invitrogen) and Wizard SV gel extraction kit.
-
bioRxiv - Molecular Biology 2019Quote: ... Fragments of 300 bp were then selected using E-Gel® SizeSelect(tm) 2 % agarose gels (Life Technologies) and amplified with 6-8 PCR cycles ...
-
bioRxiv - Genomics 2019Quote: ... Successful size-selection and enrichment were confirmed with visualization of products on a 2% agarose E-Gel (Invitrogen). Products underwent a final AMPure XP purification clean-up followed by quantification with a Qubit® 2.0 Fluorometer ...
-
bioRxiv - Neuroscience 2020Quote: ... H&E and Vimentin stained sections were scanned by EVOS FL auto 2 imaging system (Thermo Fisher Scientific). High magnification fluorescent microscopy was performed either on an Axioscope (Carl Zeiss) ...
-
bioRxiv - Genomics 2022Quote: ... and the libraries were visualized by electrophoresis on a 2% E-Gel™ EX Agarose Gels (Invitrogen, G402022). All RNA-seq libraries were sequenced on the NextSeq 1000 platform (Illumina ...
-
bioRxiv - Bioengineering 2023Quote: dPPM was stained with H&E (Hematoxylin solution, Gill no.2, Sigma Aldrich, aqueous Eosin Y, Thermo scientific) to assess cellular content and histoarchitecture ...
-
bioRxiv - Systems Biology 2023Quote: ... A size selection of the final library pool was performed on a 2% E-gel EX (Invitrogen, #G401002) to exclude small fragments (less than about 200bp ...
-
bioRxiv - Developmental Biology 2024Quote: ... size selection was performed post-amplification using 2% E-Gel Size Select II Gels (Thermo Fisher, Cat #G661012), retrieving fragments from 150 – 240 bp (representing 30 – 120 bp without the adapter sequences) ...
-
bioRxiv - Plant Biology 2020Quote: ... The luminescence was detected for 5-6h with a signal integration time of 2min using Varioskan Flash plate reader (Thermo Fisher Scientific). For determining the effects of chemical inhibitors ...
-
bioRxiv - Microbiology 2022Quote: ... Infected cells were plunge-frozen in liquid ethane-propane mix 3h or 6h post-infection using a Vitrobot plunge freezer (Thermo Fisher Scientific) at 23°C ...
-
bioRxiv - Cell Biology 2021Quote: ... the fatty acid analog C1-BODIPY 500/510 C12 was incubated for 6h at a final concentration of 2μM (Thermo Fisher Scientific, #D3823). Images were acquired on a Leica TCS SP8 equipped with a 405-nm laser line and a white light laser with a 63x/1.4 DIC Lambda blue Plan Apochrome objective ...
-
bioRxiv - Immunology 2020Quote: ... 50 µL of 2 µg/mL purified RBD-6H as described above was coated on flat-bottomed 96-well plates (Immunon 4 HBX; Thermo Fisher Scientific) overnight at 4 °C ...
-
bioRxiv - Developmental Biology 2023Quote: ... For RNAi YAP and TAZ knock down – hMSCs were starved for 6h in DMEM without antibiotics before adding siRNA for YAP or TAZ and scramble control (Stealth RNAi Invitrogen Thermo Fisher Scientific) in OptiMEM with addition of Lipofectamine 3000 (both Thermo Fisher Scientific) ...
-
bioRxiv - Developmental Biology 2022Quote: ... donated foetal tissue stored in Hibernate-E medium (Hib-E, Thermo Fisher Scientific A1247601) was collected from local maternity hospital soon after passing ...
-
bioRxiv - Bioengineering 2020Quote: ... 4’-6-diamidino-1-phenylindole (DAPI, Life Technologies) was applied at 1 μg/mL for 90 minutes to stain the nuclei and the samples were washed and mounted with AquaPoly/Mount (Polysciences) ...
-
bioRxiv - Neuroscience 2021Quote: ... propanal (ACROS Organics, 1%, CAS# 123-38-6), and 1,4-diaminobutane (ACROS Organics ...
-
bioRxiv - Immunology 2020Quote: ... 6-incubation with 1 μg/mL DAPI (Invitrogen) and 2 μg/mL Wheat Germ Agglutinin (Invitrogen ...
-
bioRxiv - Cell Biology 2022Quote: ... and 1 ng/ml IL-6 (Invitrogen, PHC3011)) (Wimmer ...
-
bioRxiv - Developmental Biology 2023Quote: α6-FITC (1:200, Invitrogen, 11-0495-82),
-
bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...
-
bioRxiv - Biophysics 2023Quote: ... Transfection lasted for 6 hours at 37°C and was terminated by replacing the medium with fresh 37°C Opti-MEM® (Life Technologies, ThermoFisher Scientific) supplemented with 50 μg/mL Gentamycin (Invitrogen) ...
-
bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 30 sec and extension at 60 °C for 30 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
-
bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 5 sec and extension at 58 °C for 34 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
-
bioRxiv - Molecular Biology 2021Quote: ... Nuclei were detected by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA).