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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 6H Benzo g 1 4 diazonino 7 6 5 cd indol 6 one 10 ethenyl 1 3 4 5 7 8 10 11 12 13 decahydro 4 hydroxymethyl 8 10 13 trimethyl 7 13 bis 1 methylethyl 4S 4R* 7R* 10S* 13S* 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: Gene silencing in INS-1 832/13 cells was performed using Lipofectamine RNAiMAX (Life Technologies, Waltham, MA) and 60 nM siRNA targeting Atraid mRNA (s151757) ...
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bioRxiv - Neuroscience 2023Quote: ... a protein expressed by neurons (1:1000, clone M13, Thermo Fisher, cat#13-1500, Waltham, MA, USA). Antibodies specificity was verified and IHC was performed as described in Mota et al21 and Assis-Mendonça et al12 ...
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bioRxiv - Molecular Biology 2023Quote: ... The dilutions for the remaining antibodies are as follows: anti-Transferrin Receptor (ThermoFisher 13-6800, 1:1000), anti-FBXL5 (ThermoFisher PA5-113529 ...
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bioRxiv - Bioengineering 2023Quote: ... Primary antibodies and dilutions are as follows: anti-CD326 (1:100, 13-5791-80, ThermoFisher, RRID: AB_1659715), anti-TER-119 (1:50 ...
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bioRxiv - Neuroscience 2023Quote: ... primary antibodies for mouse anti-MAP2 (microtubule-associated protein 2; Invitrogen, Catalog No. 13-1500, 1:1000) and guinea pig anti-synapsin1/2 (Synaptic Systems ...
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bioRxiv - Cell Biology 2021Quote: ... the cell number was determined by manually counting of 10×13 tile images (4X) using Fiji Software.27 Cells were harvested using TrypLE™ Express Enzyme (Gibco, Thermo Fisher Scientific) and subcultured in the same conditions ...
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bioRxiv - Cancer Biology 2024Quote: ... and resolved on 3-8% or 4-12% gradient SDS-PAGE gels (Invitrogen, NuPAGE). After transfer to a polyvinylidene difluoride (PVDF ...
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bioRxiv - Biochemistry 2020Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes).
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bioRxiv - Cell Biology 2022Quote: ... 1.2M sorbitol buffer (pH 7.5) and permeabilized with 1% Triton X-100 stained with 1 μg/ml DAPI (4’, 6-diamidino-2-phenylindole; Molecular Probes). Cells were imaged using a DeltaVision Ultra microscope with a 60X objective (NA = 1.42) ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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Spatial rearrangement of the Streptomyces venezuelae linear chromosome during sporogenic developmentbioRxiv - Microbiology 2020Quote: ... The nucleoids were subsequently stained for 5 min at room temperature with 7-amino-actinomycin D (1 mg/ml 7-AAD in DMSO, Thermo Fisher Scientific) diluted 1:400 in PBS buffer ...
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bioRxiv - Microbiology 2023Quote: ... The qPCR reactions for the data corresponding to Figure 7 and Figure 7 – figure supplement 1 were carried out in 384-well plates on a QuantStudio™ 5 (Applied Biosystems). The thermal cycling conditions were as follows ...
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bioRxiv - Developmental Biology 2022Quote: ... hiPSC at day 6-7 after passaging were treated with a 1:1 mixture of TrypLE Select (Life Technologies) and 0.5 mM EDTA/phosphate-buffered saline (PBS ...
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bioRxiv - Microbiology 2021Quote: ... 7-10 µg of RNA was denatured in 2x RNA load dye (Thermofisher) and heated to 65°C for 15 min ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Organoids were passaged every 7-10 days by disruption with TrypLE (Thermo Scientific) and reseeded at a concentration of 100-500 organoids per well.
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bioRxiv - Bioengineering 2021Quote: ... Samples were stained with 2’,7’-Dichlorofluorescein diacetate (10 µM) (Thermo Fisher, USA) for one hour ...
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bioRxiv - Molecular Biology 2022Quote: ... then placed in 10 μM 2′,7′-dichlorodihydrofluorescein diacetate (Invitrogen, Carlsbad, CA, USA) and incubated under vacuum for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... Neurons were cultured for 7-10 days in complete Neurobasal Plus Medium (Gibco). Mixed glial cells were cultured with the same cortical cell isolation procedure ...
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bioRxiv - Immunology 2022Quote: ... Treg cells were stimulated for 7-8 days with Dynabeads™ Human T-Activator CD3/CD28 (1:1 beads:Treg cell ratio, Gibco), and cultured in X-VIVO 15 with Gentamicin ...
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bioRxiv - Neuroscience 2022Quote: ... 1:2000) and 4′,6-diamidino-2-phenylindole (DAPI, 1:1,000, Life Technologies). Following several washes ...
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bioRxiv - Neuroscience 2022Quote: ... IL-6 (1:4-1:2 dilution, #KMC0061; ThermoFisher Scientific, Waltham, MA, USA), and NfL (1:4 dilution ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Molecular Biology 2019Quote: ... incubated at 95°C for 5 min and the supernatant loaded onto a either 3-8% Tris-acetate or 4-12% Bis-Tris gradient gels (Life Technologies).
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bioRxiv - Cell Biology 2024Quote: Vacuolar pH alterations were detected using the 5-(and-6)-carboxy-2′,7′-dichlorofluorescein diacetate (CDCFDA, Invitrogen) probe ...
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bioRxiv - Bioengineering 2019Quote: ... IL-7 at 5 ng/mL (ThermoFisher), and IL-15 at 5 ng/mL (ThermoFischer) ...
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bioRxiv - Biochemistry 2021Quote: Protein samples were separated via 10% or 4-12% SDS-PAGE (Life Technologies) and transferred onto nitrocellulose membranes (GE Healthcare Life Sciences) ...
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bioRxiv - Zoology 2020Quote: ... 2’-(4-ethoxyphenyl)-5-(4-methyl-1-piperazinyl)-23491-52-3 (Hoechst 33342, trihydrochloride, trihydrate, Life Technologies, H3570) in water for 20 min ...
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bioRxiv - Molecular Biology 2024Quote: ... Slides were blocked in 200 μL buffer W (GeoMx RNA Slide Prep FFPE-PCLN kit) for 30 minutes and incubated at 4°C overnight with SYTO 13 nucleic acid stain (500 nM; Thermo Fisher Scientific #S7575) and antibodies for pan-cytokeratin (panCK ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Cell Biology 2019Quote: ... 50 μg of enriched mitochondria were resolved approximately 6 cm into a pre-cast 4–12% Bis-Tris polyacrylamide gel (ThermoFisher Scientific). The lane was excised and cut in 8 approximately equal chunks and the proteins reduced ...
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bioRxiv - Cancer Biology 2021Quote: ... U251 cells were treated with 4 uM CellEvent Caspase-3/7 green detection reagent (Molecular probes) prior to imaging ...
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bioRxiv - Genetics 2020Quote: ... Colonies were passaged every 4 – 7 days using 3 – 5min incubation with 0.5mM UltraPure EDTA (Invitrogen) as required.
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Machine Learning Ensemble Directed Engineering of Genetically Encoded Fluorescent Calcium IndicatorsbioRxiv - Bioengineering 2023Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: 7 μM Aβ40 was incubated with varying stoichiometries of claramine and 5% PEG (50% w/w 10 kDa, Thermo Fisher Scientific) in the presence of 20 mM ThT (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Cells were washed 2x by spinning at 0.7 rcf for 5 minutes at room temperature and removing supernatant + resuspending in 10 mLs of Neuronal Basal Media (Invitrogen; 10888022) supplemented with B27 (Invitrogen ...
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bioRxiv - Cancer Biology 2022Quote: ... 5) Opal 690/anti-CD34 (1:100, QBEND/10, Invitrogen), 6 ...
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bioRxiv - Systems Biology 2023Quote: ... 5) Opal 690/anti-CD34 (1:100, QBEND/10, Invitrogen), 6 ...
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bioRxiv - Microbiology 2020Quote: ... About 8-15μg of total protein extract was resolved on NuPAGE 4-12% Bis-Tris gels (Invitrogen) using MES SDS running buffer (Invitrogen) ...
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bioRxiv - Neuroscience 2019Quote: ... 8-25 μm of protein per lane was resolved in 4-12% NuPAGE Bis-Tris gels (Invitrogen) and chemiluminescent quantitation was performed as previously described (23).
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bioRxiv - Molecular Biology 2019Quote: ... Boiled samples were loaded into 8% SDS-PAGE or NuPAGE 4% - 12% Bis-Tris protein gels (ThermoFisher). Specific protein targets were analyzed by immunoblot assay with specific antibodies listed in Table ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... anti-mouse IL-1β biotin conjugate (13-7112-81), and anti-mouse IL-6 (MP5-20F3, 14-7061-85) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... non-sun-exposed skin as previously described(13) and used at passages 2-6 and grown in Epilife medium (Gibco, #MEPI500CA) with added human keratinocyte growth supplement (10ul/ml medium) ...
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bioRxiv - Cancer Biology 2024Quote: ... Above cells were split every 4-5 days using 5-10-minute incubation with 0.05% trypsin-EDTA (Thermo Fisher) at 37 °C ...
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bioRxiv - Neuroscience 2021Quote: ... 10-30 μg of samples was loaded onto NuPAGE 4–12% Bis–Tris Midi Gel (Thermo Fisher Scientific, Cat# WG1403BOX) and electrophoresed with the NuPAGE MOPS SDS running buffer (Thermo Fisher Scientific ...
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bioRxiv - Genetics 2019Quote: ... by incubating at 70°C for 10 minutes and separated on 4-12% NuPAGE Bis-Tris gels (Thermo Fisher Scientific) along with 5% of input samples and analysed by Western blotting as described above.
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bioRxiv - Cell Biology 2020Quote: ... Laemmli sample buffer (0.1 mL of 4x) was added to lysate prior to loading 10 μL on a 4%–12% Bis-Tris NuPAGE gel (Invitrogen). Gels were transferred onto a Mini-size polyvinylidene difluoride membrane (Bio-Rad ...
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bioRxiv - Neuroscience 2020Quote: ... Cell lysates containing 10 μg of total protein were loaded (without boiling) on precast NuPAGE 4-12% Bis-Tris gels (Invitrogen) for SDS-PAGE analysis ...