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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 6 oxo 1 phenyl 1 4 5 6 tetrahydro pyridazine 3 carboxylic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: We purchased 6-mercaptopurine monohydrate (6-MP, #AC226520050) from Thermo Fisher.
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Neuroscience 2021Quote: ... Larvae (6 dpf) were transferred to a 3 cm Petri dish (Thermo Scientific) and allowed to acclimatize for 5 min before video recording ...
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bioRxiv - Cell Biology 2021Quote: ... 3 M-tumours and 6 RE-tumours conserved in RNA-later (ThermoFisher,USA) used for RNA extraction ...
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bioRxiv - Microbiology 2019Quote: ... 6 ug RNA was treated with 3 units of Turbo DNase I (Invitrogen) for 45 min at 37 °C ...
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bioRxiv - Neuroscience 2023Quote: Primary mouse microglia were plated at a density of 3×105 in 6 well plates and treated for 6 h prior to RNA extraction with Trizol (Invitrogen; Taïb et al., 2013). Adult microglia were treated as described in section 2.2.3 prior to extraction of total RNA using RNeasy Plus Mini Kit (Qiagen ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Biophysics 2023Quote: ... These grids were blotted with filter paper for 3∼6 s (100% humidity at 4 °C) in a Vitrobot Mark IV (Thermo Fisher Scientific) and vitrified in liquid ethane at liquid nitrogen temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... the samples were reconstituted in 6 uL 0.1% formic acid (FA) 0.05% heptafluorobutyric acid (HFBA) (Thermo Fisher Scientific, cat. 25003).
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bioRxiv - Cell Biology 2020Quote: ... Cells were passaged every 5 or 6 days using Versene (Gibco, A4239101). Clinical hESCs were tested weekly for mycoplasma contamination using a Myco-detection Kit (InvivoGen ...
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bioRxiv - Cell Biology 2023Quote: ... 5 Bcl6 and 6 Smyd2 KO livers using TRIzol reagent (Invitrogen #15596026) followed by purification using the RNeasy Mini kit (Qiagen #74014) ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... С6 and CHO/5-HT2C were maintained in the F12 medium (Invitrogen). In all cases ...
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bioRxiv - Bioengineering 2024Quote: ... Coupling 5-(and 6)-Carboxytetramethylrhodamine (TAMRA) mixed isomers (Thermo Scientific, Waltham, MA) and Fmoc-8-amino-3,6-dioxaoctanoic acid (Fmoc-PEG2-OH ...
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bioRxiv - Developmental Biology 2022Quote: Mice were anesthetized using isoflurane and injected with 1% Biocytin-5(and-6-)- Tetramethylrhodamine (Biocytin-TMR: ThermoFisher Scientific; Waltham, MA, Cat# T12921). After 30 minutes in circulation ...
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bioRxiv - Immunology 2022Quote: ... Media was removed from THP-1 monocytes cultures (5-6 x 105 cells/ml) and cells were washed once with DPBS (Thermo Fisher Scientifics). A total of 2 x 106 cells were resuspended in 50 μl of BTXpress solution and the Cas9-sgRNA complex was added ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were rinsed with serum-free RPMI-1640 medium before being transfected with 5 µL of siRNA pool (20 µM) and 5 µL Lipofectamine 2000 in a 6-well plate in 1 mL of antibiotic-free Opti-MEM (Gibco, #31985-062) medium plus 2 mL antibiotic-free cell-culture medium ...
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bioRxiv - Molecular Biology 2022Quote: ... siM1-4: 5’ -ACTGGTAGCTTATTAAAGATT- 3’) and the HOXA1 siRNA (5’ - AGAACTTCAGTGCGCCTTATT- 3’) were purchased from Invitrogen™ (Silencer® Select siRNA ...
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bioRxiv - Developmental Biology 2021Quote: ... transferred to 1.6 mL Eppendorf tubes and spun at 2000 rcf for 5 minutes at 4°C in an angle rotor (Thermo Scientific Fisher Heraeus Biofuge Primo R Centrifuge) ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Developmental Biology 2024Quote: ... and counterstained with 5 μg/mL of 4′,6-diamidino-2-phenylin-dole (DAPI) or propidium iodide (Molecular Probes, Life Technologies) for 10 min ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Immunology 2021Quote: ... were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads, Life Technologies) at 1 bead ...
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Microbiology 2019Quote: ... and 25 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES; Thermo Fisher), referred to as complete DMEM (cDMEM) ...
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bioRxiv - Microbiology 2020Quote: ... 10 ml 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), 24 mL 5% NaHCO3 (Gibco ...
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bioRxiv - Developmental Biology 2021Quote: ... and 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) were purchased from Gibco/Thermo Fisher Scientific (Grand Island ...
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bioRxiv - Cell Biology 2021Quote: ... Wnt3a proteins were immobilized to 2.8 μm carboxylic acid–coated Dynabeads® (cat. num. 14305D, ThermoFisher), as described before (Habib et al. ...
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bioRxiv - Immunology 2020Quote: ... The dyes were purchased as NHS ester derivatives: Alexa Fluor 405 Carboxylic Acid Succinimidyl Ester (Invitrogen), Cy3 mono-Reactive Dye Pack (GE HealthCare) ...
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bioRxiv - Genomics 2021Quote: ... Size-selection and cleanup were accomplished using CA-magnetic beads (Dynabeads® MyOne Carboxylic Acid, Invitrogen), and 11-11.5% PEG 6000 (Sigma-Aldrich © LLC) ...
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bioRxiv - Bioengineering 2024Quote: ... Concentration of C2 to C6 carboxylic acids and alcohols were analyzed by gas chromatography (Thermofisher, USA) using a Stabil-wax™ column with a length of 25 m and internal diameter of 0.2 μm ...
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bioRxiv - Biochemistry 2020Quote: ... Samples were run on 6% TBE gel in 1 x TBE (150 V, 90 min, 4°C) stained with SYBR Gold (Thermo Fisher Scientific. S11494) and InstantBlue Protein Stain (Expedeon).
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bioRxiv - Immunology 2019Quote: ... Hemocytes nuclei were then stained by incubating with 4’,6-diamidino-2-phenylindole (DAPI, 1 μg/mL) (Thermo Fisher Scientific, Rockford, IL, USA) dissolved in PBS and washed thrice with PBS ...
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bioRxiv - Bioengineering 2023Quote: ... for 30 seconds at room temperature followed by 15 min staining with 1 µg/mL 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen, cat. no. D1306) before microscopic imaging ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Genetics 2021Quote: ... 6-VIC ® or 6-PET® (Life Technologies Corporation, Carlsbad, USA). During capillary electrophoresis ...
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bioRxiv - Developmental Biology 2021Quote: ... The supernatant was collected and diluted 1:6 for BCA protein quantification (ThermoFisher #23227) and performed according to manufacturer’s guidelines ...
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bioRxiv - Microbiology 2019Quote: ... 6×His-Lsr2 was purified by binding to 1 mL Ni-NTA agarose (Invitrogen), after which the resin was collected and the bound protein was washed with binding buffer supplemented with increasing concentrations of imidazole ...
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bioRxiv - Bioengineering 2021Quote: ... The composition of 1 L MM1 (pH = 6) was 10 g glucose (Acros Organics), 5 g of (NH4)2SO4 (Lach-Ner) ...
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bioRxiv - Genomics 2021Quote: ... and 6 μl lysozyme solution (50 mg.ml-1, Thermo Fisher Scientific, Cat. No.: 90082) and incubated for 60 min at 37°C with light mixing at 300 rpm using the Eppendorf ThermoMixer® C (Eppendorf ...
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bioRxiv - Neuroscience 2022Quote: ... in 6 well-plates in 1 ml of medium 50% minimal essential medium (Gibco), 25% heat inactivated horse serum (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... 1 × 105 cells were seeded in a 6-well plate (140675, Thermo Fisher Scientific) in which glass coverslips coated with collagen IV (Nitta Gelatin ...
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bioRxiv - Cell Biology 2023Quote: ... cells were supplemented with blasticidin S (6 µg mL-1, Thermo Fisher Scientific, A1113903). The WAPL-AID cell line was supplemented with puromycin (0.5 µg mL-1 ...
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bioRxiv - Cell Biology 2020Quote: ... 5 micromols sodium sulfo-NHS and 5 micromols 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Thermo Fisher #22980) in 10 μL of DMSO ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei were detected by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA).
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bioRxiv - Molecular Biology 2020Quote: ... and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific) at 1:2,000 dilution at RT for 5 min ...