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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 6 chloro N 3 methoxypropyl pyridazin 3 amine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Immunology 2019Quote: ... The reaction was stopped with 3 N H2SO4 and read at 492 nm on a MultiSkan GO ELISA plate reader (Thermo Fisher Scientific, Vantaa).
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bioRxiv - Molecular Biology 2019Quote: ... The quantity and quality of the isolated RNA (n = 3, independent pools of fascicles from different mice) was determined with a Qubit® (1.0) Fluorometer (Life Technologies, California, USA) and a Bioanalyzer 2100 (Agilent ...
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bioRxiv - Biochemistry 2021Quote: ... Fluorescence-labelled 1,2-dioleoyl-sn-glycero-3-phosphoethanolamine-N-(7-nitro-2-1,3-benzoxadiazol-4-yl) (NBD-PE) was obtained from Molecular Probes (Eugene, Oregon, US). Sucrose was purchased from XiLong Chemical Co. ...
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bioRxiv - Systems Biology 2021Quote: ... Peptides were first loaded onto an Acclaim™ PepMap™ 100 C18 (3 μm, 75 μm ID× 20 mm length, P/N 164946 Thermo Scientific), and then separated on C18 packed emitter column (3 μm ...
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bioRxiv - Neuroscience 2022Quote: ... analyses cultured NSPCs or hippocampal tissue (at least n=3 per condition) were collected at indicated time-points for each experiment with RIPA buffer (Cat# A32963, Thermo Fisher, Waltham, USA) plus a protease inhibitor cocktail with the addition of phosphatase inhibitors (Cat#78441 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... the cell layers are examined for cell morphology using a phase contrast microscope washed with media and then 10 uM of 2-(N-[7-nitrobenz-2-oxa-1, 3-diazol-4-yl] amino)-2-deoxyglucose (2-NBDG) (Molecular Probes-Invitrogen, CA, USA) was used to assess glucose uptake in the presence or absence of 100 nM insulin as the initiating step and incubated for 20 or 30 minutes ...
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bioRxiv - Microbiology 2023Quote: ... The N concentrations were determined by dry combustion of a 3-4 mg sample with a Flash EA1112 elemental analyzer (Thermo Scientific, Rodano, Italy).
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bioRxiv - Cell Biology 2022Quote: Total RNA was extracted from the LV myocardium of 2-month-old male WT and Rbm20ΔRS mice (n = 3 per group) using TRIzol Reagent (Life Technologies, Waltham, MA, USA) and treated with DNase I (cat# D9905K ...
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bioRxiv - Neuroscience 2024Quote: ... Real-time PCRs were performed in technical and independent experiment triplicates (n=3) with Power Sybr Green Master Mix (Life Technologies, Darmstadt, Germany) on a VIIA7 (Life Technologies ...
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bioRxiv - Cancer Biology 2023Quote: ... Real-time PCR reactions were run in triplicates (n=3) in the 7500 Real-time PCR system by Applied Biosystems (Thermo Fisher Scientific) using power SYBR Green PCR master mix (Applied Biosystems ...
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bioRxiv - Neuroscience 2023Quote: ... The drill was then replaced with a micropipette loaded with 3-5nL of 0.6 M MnCl2 with 0.3mg/ml 3K rhodamine dextran amine (RDti and 0.15mg/ml 10K fluorescein dextran amine (Molecular Probes/Invitrogen/ThermoFisher) in sterile phosphate-buffered saline ...
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bioRxiv - Molecular Biology 2019Quote: ... Total RNA from HEK 293T cells (N = 2 biological replicates) or whole mouse brain (N = 6 biological replicates) was extracted using TRIzol (ThermoFisher) and treated with RNase-free DNase I (Promega) ...
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bioRxiv - Cell Biology 2020Quote: ... 5 micromols sodium sulfo-NHS and 5 micromols 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC) (Thermo Fisher #22980) in 10 μL of DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were split every 2-3 days through 9 minutes incubation with EDTA 50mM pH=7.4 and 3 minutes of 0.25% trypsin (Gibco).
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bioRxiv - Neuroscience 2021Quote: ... was performed using the Applied Biosystems QuantStudio 3 Real-Time PCR system and analyzed with the QuantStudio 3 Design and Analysis software (v1.5.1,ThermoFisher). Briefly ...
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bioRxiv - Molecular Biology 2021Quote: 3′-desthiobiotin labeling was carried following the manufacturer’s’ guidelines of Pierce™ RNA 3′ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Biophysics 2019Quote: ... Procaspase-3 (0.123 mg/mL protein extract) and caspase-3 (1.0 U) were assayed in 96-well microplates (Nunc™ MicroWell™ 96-Well ...
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bioRxiv - Evolutionary Biology 2020Quote: ... The pbf1 gene of johansen Standard was amplified with the primer pair psbNRO_F 5’-AGCATTGGGAGGCTCATTAC-3’ and psbNRO_R 5’-GGAAACAGCAACCCTAGTCG-3’ and cloned into to pCRTM2.1-TOPO® (Invitrogen). The vector was linearized with HindIII and in vitro transcription was performed according to the suppliers’ protocol ...
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bioRxiv - Microbiology 2020Quote: ... RdRp_rev 5’-CAAATGTTAAAAACACTATTAGCATA-3’ RdRp_probe 5’-FAM-CAGGTGGAACCTCATCAGGAGATGC-TAMRA-3’) and/or TaqMan gene expression assays (Applied Biosystems) for ACTB (Hs99999903_m1) ...
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bioRxiv - Bioengineering 2021Quote: ... Celsus Laboratories) was reacted with peptide-hydrazides using 1-ethyl-3-[3-dimethylaminopropyl] carbodiimide hydrochloride (EDC, ThermoFisher Scientific) in 0.1 M MES [2-(N-morpholino)ethanesulfonic acid] buffer with 8 M urea (Sigma ...
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bioRxiv - Microbiology 2022Quote: ... 3 µL RT reaction mix consisting of 3 µL of 50 µM random hexamers (Thermo Fisher, cat#48190011), 0.8 µL of 25 mM deoxyribonucleotide triphosphates (dNTPs ...
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bioRxiv - Genomics 2021Quote: 3’-desthiobiotin labeling was carried following the manufacturers’ guidelines of Pierce™ RNA 3’ End Desthiobiotinylation (ThermoFisher, #20163). Briefly ...
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bioRxiv - Immunology 2020Quote: ... and 50 µL of 50 mg/mL 1-ethyl-3-[3-dimethyl-aminopropyl]-carbodiimidehydrochloride (Thermo Fisher Scientific, 22981) were simultaneously added to the reaction tubes ...
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bioRxiv - Immunology 2022Quote: ... and their corresponding FAM-labelled MGB probes (εGLT: 5′-AGGCACCAAATG-3′ and γ1GLT: 5 ′-CTCAGCCAGGACCAAG-3′) (Applied Biosystems) were designed in house ...
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bioRxiv - Neuroscience 2023Quote: ... 5’- CCACCCTCCAGCCAATC-3’ and FAM/ZEN-labeled probe 5’-ACAGGGCAACCATTGGCTCG-3’ with Taqman Gene Expression Master Mix (ThermoFisher).
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bioRxiv - Molecular Biology 2023Quote: The RNA of 500 µL iRBCs at 3-5% parasitaemia was isolated using 3 mL TRIzol reagent (Invitrogen) followed by phenol-chloroform phase separation ...
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bioRxiv - Immunology 2023Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Microbiology 2023Quote: The embB region containing codon 406 was amplified using PCR primers F1 5’-TGATATTCGGCTTCCTGCTC-3’ and R1 5’-TGCACACCCAGTGTGAATG-3’ designed using Primer3 (23) (version 0.4.0) and acquired from ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting topoisomerase 2beta (5’CGAUUAAGUUAUUACGGUUtt 3’, s106; 5’ GAGUGUACACUGAUAUUAAtt 3’, s108; both purchased at Ambion/ThermoFIsher Scientific), TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’ ...
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bioRxiv - Cell Biology 2023Quote: ... siRNA targeting topoisomerase 2beta (5’CGAUUAAGUUAUUACGGUUtt 3’, s106; 5’ GAGUGUACACUGAUAUUAAtt 3’, s108; both purchased at Ambion/ThermoFIsher Scientific), TDP2 (5’ GUGGUGCAGUUCAAGAUCAtt 3’ ...
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bioRxiv - Cancer Biology 2024Quote: ... we isolated mRNA from bone marrow of 3 Ctrl and 3 cKO animals using TRizol (Thermo Scientific, #15596026) and prepared cDNA using 500 ng of total RNA using high capacity cDNA reverse transcriptase (Thermo Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 4.0 × 107 beads were activated with 1-ethyl-3-(3-dimethylaminopropyl)carbodiimide (EDC, Thermo Fisher Scientific [TFS] 22980) and N-hydroxysuccinimide (NHS ...
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bioRxiv - Cell Biology 2020Quote: ... and ArC Amine Reactive Compensation Beads (Invitrogen, A10346) according to manufacturer’s instruction using matching fluorophore-conjugated antibodies to the antibodies used for each instance ...
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bioRxiv - Cell Biology 2020Quote: ... siPORT™ amine (8.75 µl, Cat#AM4503, Invitrogen) and the silencer select siRNA (6 µl ...
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bioRxiv - Microbiology 2022Quote: ... and ArC Amine Reactive Compensation beads (ThermoFisher Scientific) and a known volume of CountBright absolute beads (ThermoFisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... All tracers were conjugated to dextran amine (Invitrogen) and had similar transport properties (85).
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bioRxiv - Bioengineering 2021Quote: ... maleic anhydride amine-binding wells (Thermo Fisher, 15100) were washed three times with PBS containing 0.05% Tween-20 ...
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bioRxiv - Immunology 2019Quote: ... and ArC™ Amine Reactive Compensation beads (Thermofisher) and manually inspected for representative samples ...
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bioRxiv - Physiology 2021Quote: ... siPORT™ amine (8.75 µl, Cat#AM4503, Invitrogen) and the silencer select siRNA (6 µl ...
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bioRxiv - Biophysics 2019Quote: 108 amine coated Dynabeads M270 (Thermo Fisher Scientific) were washed in PBS buffer pH 7.4 and incub ated in a PBS solution containing 1 % v/v glutaraldehyde for 1 h in a rotator at 18 rpm (L abnet) ...
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bioRxiv - Immunology 2023Quote: ... and ArC™ Amine Reactive Compensation Beads (Invitrogen) served as a loading control for live/dead cell staining ...
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bioRxiv - Immunology 2023Quote: ... and ArCTM Amine Reactive Compensation Bead (Thermo Fisher) individually stained with each fluorochrome ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: An amine-based protein labeling kit (Invitrogen, #A20173) was used for labeling DUNP19 with AlexaFluor-647 or AlexaFluor-594 ...
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bioRxiv - Microbiology 2020Quote: ... analyzed on a QuantStudio 3 (Applied Biosystems) thermal cycler using the standard cycling conditions ...
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bioRxiv - Biophysics 2021Quote: ... supplemented with 3 mM L-glutamine (Gibco), 0.25 mg/mL gentamycin (Gibco) ...
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bioRxiv - Molecular Biology 2020Quote: ... and 3% O2 in DMEM/F12 (Gibco) with B27 supplement (Gibco) ...
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bioRxiv - Microbiology 2020Quote: ... then 3-mm glass beads (Fisher Scientific) and 1 ml fecal lysis buffer (50 mM Tris pH7.6 ...
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bioRxiv - Neuroscience 2021Quote: ... 3 % goat serum (with 0.02 % NaN3) (Invitrogen), 3 % donkey serum (Millipore ...
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bioRxiv - Neuroscience 2021Quote: ... and run on a QuantStudio 3 (ThermoFisher). All reactions were normalized to the housekeeping gene RpL32 and control flies ...