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Citations for Thermo Fisher :
651 - 700 of 10000+ citations for 6 Oxa 7 azatricyclo 3.3.0.02 4 oct 7 ene 8 1 methylethyl 9CI since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2020Quote: ... and incubated in cell* dishes at 37° C for 4 – 7 hours before adding cell culture medium (RPMI 1640, -glucose +glutamine, Gibco), supplemented with penstrep (10,000 U/ml penicillin / 10,000 μg/ml streptomycin ...
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bioRxiv - Immunology 2022Quote: ... 5-7 × 106 cells were resuspended in 3 mL of FACS buffer (4% FBS in phosphate buffered saline (PBS, Gibco)) and sorted at the University of Massachusetts Amherst Flow Cytometry Core Facility using a BD FACSAria Fusion (Becton Dickinson) ...
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bioRxiv - Neuroscience 2022Quote: Larval fish (4-7 dpf) were mounted in a small drop of 2% low melting point agarose (ThermoFisher Scientific 16520) in the center of the uncoated side of a mirror galvanometer (Thorlabs GVS0111) ...
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bioRxiv - Neuroscience 2021Quote: ... 4’,6-Diamadino-2-phenylindole (DAPI, 1:1000, Invitrogen) was incubated after secondary antibody incubation for 15 min at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (Invitrogen, 1:1000), Mouse antiglial fibrillary acidic protein (GFAP ...
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bioRxiv - Cancer Biology 2020Quote: ... HBEC3-KT cells were incubated with 1 μM of 2’-7’-dichlorofluorescin diacetate (CM-H2DCFDA; Invitrogen) for 30 min at 37°C ...
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bioRxiv - Molecular Biology 2022Quote: ... Cultures were passaged every 5 to 7 days with collagenase type IV (Invitrogen; 1 mg/mL). The identities of all parental hESC and hiPSC lines were confirmed by DNA fingerprinting and all cell lines were regularly tested to exclude mycoplasma contaminations using a PCR-based assay ...
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bioRxiv - Microbiology 2021Quote: ... 1% Penicillin/Streptomycin and 7% of heat-inactivated fetal bovine serum (FBS) (all from ThermoFisher Scientific). VeroE6/TMPRSS2 cells (NIBSC 100978 ...
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bioRxiv - Developmental Biology 2021Quote: ... 1 minute at 60°C)] on a QuantStudio 5 or QuantStudio 7 qPCR machine (Applied Biosystems). For qPCR assessments ...
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bioRxiv - Biochemistry 2023Quote: ... 1 mM DTT using ‘SnakeSkin’ dialysis tubing with a 7 kDa molecular weight cut-off (ThermoFisher). His6-tagged cleavage products as well as TEV protease were removed with Ni-NTA agarose resin (Qiagen ...
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bioRxiv - Cell Biology 2023Quote: ... Integrin αV was stained with a commercial antibody (ThermoFisher, USA, clone RMV-7, 1:1000 diluted). Labeling of primary antibodies was visualized with anti-mouse PE and anti-rat PE (both Southern Biotech ...
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bioRxiv - Synthetic Biology 2023Quote: ... Huh-7 and TCMK-1 cells were maintained in high glucose DMEM media (Gibco, 41966-029) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2024Quote: ... Anti-CD49-PerCPefluor710 (TS2/7) and anti-PD-1-PeCy7 (eBioJ105) were supplied by Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... mice were sacrificed 24 h post exposure to Cl2 or air and lavaged as described previously(7, 8) Total protein concentrations in cell-free lavage were measured with the BCA Protein Assay Kit (#23225; Thermo Fisher Scientific, Rockford, IL). Cells were re-suspended in 100μl of phosphate buffered saline (PBS ...
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bioRxiv - Neuroscience 2023Quote: ... saline (N = 7) or muscimol (N = 8, conjugate of muscimol and the Bodipy TMR-X fluorophore, InvitrogenTM; purchased from Thermo Fisher Scientific, reference M23400) disolved in PBS (ph 7.4 ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Immunology 2021Quote: Total RNA of THP-1 monocytes derived macrophages cultured in different circumstance for 7 days was digested from 6-well plates using trypsin and then TRIzol™ Reagent (Cat. #15596026, ThermoFisher Scientific, USA) was added ...
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bioRxiv - Developmental Biology 2019Quote: The compound 5-(and-6)-carboxy-2’,7’-dichlorodihydrofluorescein diacetate (carboxy-H2DCFDA, Image-iT LIVE Green Reactive Oxygen Species Detection Kit, Molecular Probes; Invitrogen, I36007) was used to visualise the in vivo production of ROS ...
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bioRxiv - Plant Biology 2022Quote: ... Each sample was incubated with 50 µm of 2’,7’-Bis-(2- carboxyethyl)-5-(6)-carboxyfluorescein acetoxymethyl ester (BCECF-AM; Molecular Probes, Eugene, OR) at 28°C ...
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bioRxiv - Immunology 2023Quote: ... except for Fig 6 and Fig 7 which the recovered cells were counted by inclusion of counting beads (Accu-Check, Molecular Probes, Thermo Fisher Scientific) in the FACS sample according to manufacturer’s instructions and calculated using the equation ...
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bioRxiv - Biophysics 2022Quote: ... N-(7-Nitrobenz-2-Oxa-1,3-Diazol-4-yl)-1,2-Dihexadecanoyl-sn-Glycero-3-Phosphoethanolamine (NBD-PE) was purchased from ThermoFisher (Waltham, MA). Cholesterol Oxidase from Streptomyces was purchased from MP Biomedicals (Santa Ana ...
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bioRxiv - Cancer Biology 2022Quote: ... To count the apoptotic events cells were treated with a fluorescent dye detecting Casp3/7 activation (CellEvent™ Caspase-3/7 Green Detection Reagent, Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Immunology 2022Quote: ... in the presence of T cells at a 1:1 effector:target cell ratio in the presence of the caspase-3/7 Green Detection Reagent (Invitrogen). Images were taken every 2 h at 10× magnification ...
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bioRxiv - Physiology 2019Quote: Total RNA samples were extracted from one thousand 4-7 day-old Culex female antennae with TRIzol reagent (Invitrogen, Carlsbad, CA). Antennal cDNA was synthesized from 1 µg of antennal total RNA from each species using iScript cDNA synthesis kit (Bio-Rad ...
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bioRxiv - Immunology 2022Quote: About 80,000 MCF-7 cells were placed in each chamber of a 4-well chamber slide (Thermo Scientific, cat. no. 177399) containing 500 μl of Dulbecco’s Modified Eagle Medium (DMEM ...
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bioRxiv - Microbiology 2020Quote: The in vitro enzymatic assay with the fluorogenic substrate ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, UbiQ) was assembled in a 384-well microplate (Thermo Scientific). Assays were performed in 20 μL reaction volumes and in triplicate ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
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bioRxiv - Microbiology 2022Quote: ... with 250ng each of the 8 plasmids corresponding with each segment of viral genome (or 7 plasmids for mock) and 4 µl Lipofectamine 2000 transfection reagent (Thermo Fisher) per well ...
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bioRxiv - Molecular Biology 2023Quote: ... 20 μl was loaded on to NuPAGE 4 to 12% Protein Gels with 7 μl of 4X NuPAGE LDS sample buffer as described by the manufacturer (Invitrogen: NP0322BOX) and run for 2 h at 100 V ...
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bioRxiv - Neuroscience 2023Quote: Third instar larval brains were dissected in phosphate buffered saline (PBS) and fixed with 4 % formaldehyde (FA) pH 7 (Thermo Scientific)/PBS for 20 minutes at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
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bioRxiv - Microbiology 2023Quote: For detection of NO in treated mycobacteria DAF-FM diacetate (4-Amino-5-Methylamino-2’,7’-Difluorofluorescein),27 purchased from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
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bioRxiv - Plant Biology 2020Quote: ... Data acquisition and quantification was performed with Chromeleon 7 (Thermo Scientific).
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bioRxiv - Biophysics 2021Quote: ... cells were harvested at day 7 using trypsin-EDTA (Fisher Scientific) and centrifugation (320g ...
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bioRxiv - Cell Biology 2020Quote: ... on a QuantStudio 7 Flex real-time PCR detector (Thermo Fisher). Relative mRNA levels were normalized to those of GAPDH mRNA in each reaction and undetermined raw Ct values were set to 40 for analysis purposes ...
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bioRxiv - Evolutionary Biology 2021Quote: ... on a Viia 7 Real-Time PCR system (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... 7 µL of molecular grade water (Fisher Scientific UK Ltd, UK) and 2 μL of DNA template at the original sample concentration ...
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bioRxiv - Molecular Biology 2020Quote: ... in a QuantStudio 7 Flex Real-Time PCR System (Applied Biosystems). All primers were used at 10 μM and sequences can be found in Table 1 ...
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bioRxiv - Cell Biology 2022Quote: ... on the ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). Each biological replicate was measured in technical duplicates ...
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bioRxiv - Plant Biology 2019Quote: ... in a ViiA 7 Real-Time PCR System (Thermo Fisher Scientific). Ct values for the gene of interest are expressed relative to that Ct value found for primers targeted to AT1G13320 ...
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bioRxiv - Cancer Biology 2020Quote: ... and then 10 μM 2’,7’-dichlorodihydrofluorescein diacetate (H2DCFDA) (Life Technologies) was added to the cells and they were incubated for one hour ...
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bioRxiv - Immunology 2019Quote: ... the CellEvent Caspase-3/7 Green Detection Reagent (Thermo Fisher Scientific) was added to the cells with complete media for 30 min in 37°C and the caspase-3/7 positive cells were measured on an Attune NXT flow cytometer (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... and the ViiA 7 Real-time PCR system (Thermo Fisher Scientific). Primers used in this study are listed in Suppl ...
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bioRxiv - Cancer Biology 2019Quote: ... CellEvent™ Caspase-3/7 Green Flow Cytometry Assay Kit (Invitrogen) was used according to the manufacturer’s protocol with modest modifications ...
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bioRxiv - Biochemistry 2019Quote: ... The coding region for MCM3-7 were cloned into pFastbac (ThermoFisher). MCM3 was cloned with an N-terminal FLAG tag (DYKDDDDK ...
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bioRxiv - Synthetic Biology 2019Quote: ... and 7 µl of 100X Halt protease inhibitor (Thermo Fisher Scientific). After 15 min of thawing and resuspension ...
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bioRxiv - Physiology 2019Quote: ... QuantStudio 7 Flex Real time PCR system (Applied Biosystems Life Technologies)