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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 6 Nitro 1H Indazole 3 Methanol since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (Invitrogen; SNN1008). Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2023Quote: ... they were probed for 1h at RT with the fluorescent secondary antibodies (1:1000: ThermoFisher). Cell nuclei were stained with Hoechst 33342 (10 μg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... during 1h at room temperature before mounting in ProLong Gold Antifade reagent (P36941, Life Technologies). F-Actin was stained using Actistain (1:150 ...
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bioRxiv - Cell Biology 2023Quote: ... for 1h at 37°C and then with Proteinase K (Fisher Scientific, #25-530-049) for 2h at 55°C ...
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bioRxiv - Genetics 2023Quote: ... transiently transfected HEK293T cells were starved for 1h in methionine-free RPMI (Thermo Fisher Scientific) and subsequently pulsed for 30 minutes in starvation medium supplemented with 200µCi/mL EasyTagTM EXPRESS 35S protein labeling mix (Perkin Elmer) ...
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bioRxiv - Genomics 2024Quote: ... for 1h at 37°C and Proteinase K Solution (20 mg/mL, Thermo Fisher Scientific) for 1h at 55°C ...
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bioRxiv - Microbiology 2023Quote: ... for 1h and revealed using a 1/300 dilution of FITC-conjugated streptavidin (SNN1008, Invitrogen). Staining of DNA was performed on fixed cells by incubating them for 5 min in a 1 μg/ml 4,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cell Biology 2023Quote: ... siARP2/3 (5’- GGAUUCCAUUGUGCAUCAAtt-3’, 5’-GGGAUGAUGAGACCAUGUAtt-3’, 5’- AAAUCCUAAUGGAGACAAAtt-3’, Ambion)
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bioRxiv - Cancer Biology 2021Quote: ... Culture medium was refreshed every 2–3 days and organoids were passaged 1:2–1:6 every 7–21 days using TrypLE Express (Thermo Fisher). For co-culturing ...
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bioRxiv - Bioengineering 2022Quote: Primary neonatal cardiomyocytes were isolated from C57BL/6 mouse neonates on postnatal days 3-5 using the Pierce Primary Cardiomyocyte Isolation Kit (Thermo Fisher) as previously described (14) ...
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bioRxiv - Developmental Biology 2019Quote: ... blastocysts were collected from uterine horns and put on culture for 3-6 days in ES derivation medium composed of GlutaMAX/DMEM (Gibco, 31966), 15% FBS (Biowest) ...
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bioRxiv - Cell Biology 2019Quote: The staining of limiting membrane of the yeast vacuole was achieved with FM™ 4-64 Dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) (Invitrogen). Cells were grown to early-log phase in appropriate medium ...
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bioRxiv - Systems Biology 2019Quote: A mate-pair library with 3-6 kb fragments was prepared from pure genomic DNA using Ion TrueMate Library Reagents (Life Technologies). The Ion PGM™ template OT2 400 kit (Life Technologies ...
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bioRxiv - Cell Biology 2021Quote: ... were transiently transfected into 8 × 104 U2OS 2-6-3 cells in 4-well chamber slides using Lipofectamine 2000 reagent (Invitrogen, 11668019) according to the manufacturer’s instructions.
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bioRxiv - Cell Biology 2022Quote: ... Embedded tissues were cut into 3-6 µm sections using a paraffin microtome (Leitz) and placed on SuperFrost® Plus slides (Menzel Gläser, Thermo Scientific). Deparaffination and antigen retrieval was done as described previously (Link et al. ...
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bioRxiv - Neuroscience 2020Quote: ... All 27 samples from 3 groups and 6 GIS were divided and labeled using two sets of 5 mg 11 plex TMT reagents (Thermo Scientific A34808 ...
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bioRxiv - Immunology 2022Quote: ... and the wells were washed five times with wash buffer before the addition of 2,2’-azino-bis (3-ethylbenzothiazoline-6-sulphonic acid (ABTS, #37615, Thermo Fisher Scientific). Optical density was read 40 min later at 405 nm using a plate reader (SpectraMax i3 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Cell Biology 2021Quote: ... The same amount of protein lysate was aliquoted into different PCR tubes and simultaneously subjected to 6 different temperatures (37, 41, 45, 49, 53, 57 °C) for 3 min in the Veriti Thermal Cycler (Applied Biosystems). After 3 min cooling on ice ...
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bioRxiv - Cell Biology 2022Quote: ... the cells were expanded to 6 × 106/mL the day before transfection and were then diluted to 3 × 106/mL by ExpiCHO™ expression medium (Gibco). Plasmids were diluted to 20 μg/mL by cold OptiPRO medium (Gibco ...
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bioRxiv - Evolutionary Biology 2022Quote: ... was added to cDNA and the DNA was denatured at 95°C for 3 min prior to loading in Novex 6% TBE-urea gel (Thermo Fisher). Samples were separated for 45 min at 180 V ...
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bioRxiv - Neuroscience 2022Quote: ... 3.5 cm) containing 30 larvae and a plastic-covered stirrer (magnetic stir bar micro PTFE 6 mm x 3 mm; Fisher Scientific) were placed on a magnetic stir plate (Variomag Poly 15 stirrer plate ...
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bioRxiv - Microbiology 2023Quote: ... Total DNA was stained using 4’,6-diamidino-2- phenylindole (DAPI) diluted 1:10000 in PBS containing 3% BSA (Molecular Probes) to illuminate host cell nuclei ...
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bioRxiv - Neuroscience 2023Quote: HEK-293T cells were seeded on 6-well culture plates at 3 x 106 cells/well and grown in Dulbecco modified Eagle medium (DMEM; Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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Changes in cell morphology and function induced by NRAS Q61R mutation in lymphatic endothelial cellsbioRxiv - Cell Biology 2023Quote: ... NM_002524.5) with a 3’ terminal 6 × His-tag was chemically synthesized and cloned into the pFastBac1 vector (Invitrogen, Carlsbad, California, USA). cDNA of NRASQ61R was cloned into the pLVSIN-EF1α-AcGFP-C1 vector (Takara Bio Inc. ...
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bioRxiv - Cancer Biology 2024Quote: ... The samples were subjected to 6 rounds of 10 sec sonication each at power 3 in a 550 Sonic Dismembrator (Fisher Scientific). Between each round of sonication ...
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bioRxiv - Neuroscience 2023Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Cancer Biology 2023Quote: ... and TNFα (10 ng/ml) for 6 h and stained for cleaved Caspase 3/7 green (5 µM) and propidium iodide (2 µM) (Thermo Scientific) for an additional 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Neuroscience 2024Quote: Determination of sIL-6R was performed in the FN of NL and 21 dpi of aged WT and GFAP-IL6Tg (n=3-6/experimental group) using a precoated mouse ELISA kit (EMIL6RA, Fisher Scientific) and following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2023Quote: 1.3 million HEK293T cells were seeded in a 6 cm dish in 5 ml of DMEM [high glucose DMEM (Gibco, 12800), 3.7 g/L NaHCO3 ...
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bioRxiv - Cell Biology 2024Quote: ... hansenii cells were concentrated 10x in 200 μL YPD containing 40 μM FM4-64 dye (N-(3-Triethylammoniumpropyl)-4-(6-(4-(Diethylamino) Phenyl) Hexatrienyl) Pyridinium Dibromide) dye (Invitrogen™) to label endosomes for 8 minutes ...
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bioRxiv - Neuroscience 2024Quote: ... 20 ng/mL Interleukin-6 (IL-6) (Thermo Fisher Scientific), 20 ng/mL Interleukin-3 (IL-3 ...
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bioRxiv - Genomics 2020Quote: HepG2 and S2 cells were fixed in 1% methanol-free formaldehyde (Thermo Scientific, 28906) in D-MEM (for HepG2 cells ...
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bioRxiv - Biochemistry 2019Quote: ... All solvents (methanol, chloroform, water) were of HPLC grade and obtained from Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... HCHO solution (37%, with methanol stabilizer) was from Fisher Scientific (Cat. No. BP531-500). Tripeptides used for in vitro assays and growth experiments (H-Ala-Pro-Ala-OH ...
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bioRxiv - Cell Biology 2021Quote: ... Sample was then transferred to a methanol-activated polyvinylidene difluoride (PVDF) membrane (ThermoFisher, 22860) in the transfer buffer at 18 V for 50 min ...
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bioRxiv - Physiology 2020Quote: ... USA) previously incubated in 100% methanol followed by UltraPure water (Invitrogen, Thermo Fisher Scientific) in a Western blotting chamber containing a transfer buffer consisting of 25 mM Tris-Base ...
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bioRxiv - Physiology 2020Quote: ... USA) previously incubated in 100% methanol followed by UltraPure water (Invitrogen, Thermo Fisher Scientific) in a Western blotting chamber containing a transfer buffer consisting of 25 mM Tris-Base ...
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bioRxiv - Cancer Biology 2020Quote: ... PBS was removed and replaced with ice cold methanol (Fisher Scientific, Cat# A935-4) to fix cells ...
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bioRxiv - Molecular Biology 2020Quote: ... cells were cross-linked using final concentration 1% methanol-free paraformaldehyde (Thermo Fisher Scientific) for 8 minutes quenched by 125mM final concentration glycine for 10 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were permeabilized for 8 minutes at -20ºC in Optima Methanol (ThermoFisher, A456-1)and washed in PBS ...
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bioRxiv - Biochemistry 2021Quote: ... 80% (v/v) LC/MS grade methanol/water (Fisher Scientific W6-1, A456-1) cooled to −80°C ...
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bioRxiv - Cancer Biology 2022Quote: ... the proteins were transferred to a methanol activated PVDF membrane (Fisher Scientific, Cat. # PI88518). Electroblotting was done at 100V ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 mL dry ice-cold extraction solvent (80:20 methanol (Thermo Fisher A456):water (Thermo Fisher W6500) ...
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bioRxiv - Genetics 2021Quote: ... Metabolites within the cell pellet were extracted with 80% HPLC-grade methanol (Fisher Scientific) and 20% mass-spec (MS)-grade water as previously described (54) ...
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bioRxiv - Cancer Biology 2020Quote: ... fixed in methanol and stained using antibodies for CD45 (ThermoFisher Scientific; 14-0451-82), CD11b (Novus Biologicals ...
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bioRxiv - Biochemistry 2021Quote: ... LC/MS grade methanol and acetonitrile were obtained from Fisher Scientific (Hampton, NH, USA). Formic acid ...