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Citations for Thermo Fisher :
101 - 150 of 10000+ citations for 6 Isopropyl 7 methoxy 1 tetralone since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Immunology 2019Quote: ... stained with 7-AAD (Thermo Fisher, #A1310; 1:1000) to identify dead cells ...
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bioRxiv - Neuroscience 2020Quote: ... CAMKII-Phospho-286/7 (1:1000; 22B1; Thermo Fisher), TUJ1 (1:1000 ...
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bioRxiv - Genomics 2020Quote: ... and with 7-AAD (Invitrogen #A1310, 1:200 dilution) for 5 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1 mM 7-Aminoactiomycin D (Thermo Fisher Scientific) and analysis by Flow Cytometry using a LSRFortessa X20 (BD Biosciences).
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bioRxiv - Immunology 2022Quote: ... 7-AAD (ThermoFisher, Cat# 00-6993-50, 1:20), PE/Dazzle 594 CD21 (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... Once 1 uL of 7-AAD (Thermo Fisher Scientific) was added ...
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bioRxiv - Bioengineering 2023Quote: ... 1:500 of 7-AAD (Invitrogen, #00-6993-50) nuclei staining solution was added and nuclei were gated from the debris (Supplementary Figure 7 ...
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bioRxiv - Molecular Biology 2021Quote: ... HPLC grade methanol and isopropyl alcohol were purchased from Fisher Scientific (Pittsburgh, PA). Deionised water was collected from a cartridge-deioniser from MilliQ (Millipore ...
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bioRxiv - Microbiology 2022Quote: ... expression was induced with addition of Isopropyl-β-D-thiogalactopyranoside (IPTG) (Fisher Scientific) and the cultures were grown for an additional 20 hours at 18 °C.
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bioRxiv - Developmental Biology 2022Quote: ... and inhibitors (50 µM 5-(N-ethyl-N-isopropyl)-Amiloride (EIPA; Fisher Scientific), 8 µM EHop-016 (Selleck Chemicals) ...
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bioRxiv - Cancer Biology 2022Quote: ... iso-pentane and isopropyl alcohol were obtained from Fisher Scientific (Waltham, MA, USA).
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bioRxiv - Biophysics 2024Quote: ... IPTG (isopropyl-β-D-thiogalactopyranoside) and β-mercaptoethanol were purchased from Fisher Scientific. Amine reactive PEG (mPEG-succinimidyl valerate MW 5000 ...
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bioRxiv - Microbiology 2021Quote: ... Induction of genes on plasmids was accomplished by the addition of 0.5 mM Isopropyl β- D-1-thiogalactopyranoside (IPTG) (Thermo Fisher), 0.2% arabinose (Sigma) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Protein expression was induced by the addition of 50 μM Isopropyl β-D-1-thiogalactopyranoside (Fisher Scientific, Fair Lawn, NJ USA) to bacterial cell cultures for 2.5 hours with shaking at 37°C ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were incubated on ice for 15 min and induction was initiated with the addition of 0.4 mM isopropyl ≥-D-1-thiogalactopyranoside (IPTG) (Fisher Scientific; BP1755). Proteins were produced for 16-18 hr at 18°C.
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 [1-301] fragment N-terminally fused to a 6xHis tag was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-10N N-terminally fused to MBP was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2023Quote: The expression of NPP-19 full length N-terminally fused to GST was induced by the addition of 1 mM of isopropyl β-D-thiogalactopyranoside (IPTG) to 1 L Luria Broth cultures of exponentially growing Escherichia coli BL21 DE3 pLysS (Invitrogen) strain (OD = 0.6) ...
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bioRxiv - Cell Biology 2020Quote: ... then loaded with 2’,7’-bis-(2-carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethylester (BCECF-AM, 1.6 μM, Life Technologies) for 30 min at 37°C ...
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bioRxiv - Immunology 2021Quote: ... 2020) and reactions were carried out using a QuantStudio 6 and 7 Flex Real-Time PCR system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Evolutionary Biology 2021Quote: One hundred and fifty tergal gland segments (Segment 7) and control segments (Segment 6) of Dalotia males were dissected in EBSS (Earle’s Balanced Salt Solution; ThermoFisher) and transferred to into 150 µl ice-cold Schneider’s Drosophila medium and fetal bovine serum (SDM+FBS ...
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bioRxiv - Microbiology 2022Quote: ... or in 2 ml liquid MSgg with 7-d-old tomato seedlings in a 6-well plate (Thermo Scientific). Each well contained one seedling ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: Liver-Chips were stained in the upper channel with 5(6)-Carboxy-2′,7′-dichlorofluorescein diacetate (CDFDA) (Thermo Fisher) to visualize bile canaliculi and MRP2 activity ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were then plated at 1.6 × 106 cells per 6-well and allowed to differentiate for 5-7 days in RPMI-1640 medium (GIBCO), 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Neuroscience 2022Quote: ... of 0.5% low-melting agarose (FMC) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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Vitamin D signaling orchestrates skeletal muscle metabolic flexibility by regulating its fuel choicebioRxiv - Physiology 2023Quote: ... Quantitative RT– PCR was performed using the QuantStudio-6 and -7 Flex Real-Time PCR Systems (Thermo Fisher Scientific) with SYBR® Premix Ex Taq (Tli RNase H Plus ...
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bioRxiv - Immunology 2022Quote: ... All samples were prepared in triplicate and run on a QuantStudio 6/7 Flex Real-Time PCR System (ThermoFisher). Primer sequences for designated HHV-6 marker (U31 ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-23,36-39 (Supplementary Methods Table 2 ...
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bioRxiv - Cell Biology 2023Quote: ... Results were analyzed with the Design and Analysis Software QuantStudio 6/7 Pro systems (Thermo Fisher Scientific, version 2.6). Primers 20-59 (Supplementary Methods Table 2 ...
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bioRxiv - Cancer Biology 2023Quote: ... normalized to the expression of GAPDH on a QuantStudio 6 and 7 Pro real-time PCR system (Applied Biosystems).
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bioRxiv - Cell Biology 2022Quote: ... and 7-AAD (7-Aminoactinomycin D) (Thermofisher, # A1310) was used for DNA staining ...
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bioRxiv - Cell Biology 2022Quote: ... DNP (EM 1:6-1:30, #71-3500, Invitrogen), DPP4 (IF/FACS 1:100 ...
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bioRxiv - Synthetic Biology 2022Quote: ... 3,4-dihydroxystyrene and 2-methoxy-4-vinylphenol concentrations were quantified using a Dionex UltiMate 3000 HPLC (Thermo Scientific) with a Discovery® HS-F5-3 column (15 cm x 2.1 mm ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Microbiology 2020Quote: ... and induced with isopropyl b-D thiogalactoside (IPTG) in 1L luria broth (Thermofisher Scientific) containing ampicillin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2020Quote: ... Bacteria were treated with 0.1 mM Isopropyl β-D-thiogalactoside (IPTG) (Thermo Fisher, AM9462) at 16°C for 16 hr to induce protein expression ...
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bioRxiv - Plant Biology 2019Quote: ... with induction by 0.5 mM IPTG (Isopropyl β-D-1-Thiogalactopyranoside, 16°C, 12 h) and then purified using Ni-NTA His binding resin (Thermo Scientific) according to the manufacturer’s manual ...
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bioRxiv - Biophysics 2021Quote: ... Cells were induced with 1mM Isopropyl β-D-1-thiogalactopyranoside (IPTG) for 4 hours and harvested by centrifugation (Sorvall LYNX 6000, ThermoFisher Scientific) at 8,000 rpm for 10 minutes ...
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bioRxiv - Systems Biology 2020Quote: ... Clone cultures were concentrated to 20-fold in M9 buffer and 10 μL was plated onto a well of a 96 well plate containing NGM agar with 2 mM Isopropyl β-d-1-thiogalactopyranoside (IPTG, Fisher Scientific). Plates were dried and stored at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were incubated on ice for 15 min and protein expression was initiated with the addition of 0.4 mM isopropyl β-D-1-thiogalactopyranoside (IPTG) (Fisher Scientific; BP1755). Cells were then incubated for 16–18 hr at 18°C for optimal protein production.
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bioRxiv - Cell Biology 2020Quote: ... Quantitative real-time PCR was run on a ViiA 7 or QuantStudio 6 Flex PCR thermal cycler (Thermo Fisher Scientific) using SensiMix™ SYBR® No-ROX Kit (Bioline ...
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CHC22 clathrin mediates traffic from early secretory compartments for human GLUT4 pathway biogenesisbioRxiv - Cell Biology 2019Quote: ... Differentiation of confluent hSkMC-AB1190 myoblasts and hSkMC-AB1190-GLUT4 myoblasts was induced by incubating the cells in differentiation medium for 6 to 7 days: DMEM (Gibco), Gentamycin 50 µg/ml (Gibco ...
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bioRxiv - Neuroscience 2020Quote: ... of 0.5% low-melting agarose (FMC; Pignata et al., 2019) containing a 6:7 ratio of spinal cord medium (MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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bioRxiv - Neuroscience 2020Quote: ... 100-µl drop of 0.5% low-melting agarose (FMC, Fig. 1D,E, Fig. S1G) containing a 6:7 ratio of spinal cord medium [MEM with Glutamax (Gibco) supplemented with 4 mg/ml Albumax (Gibco) ...
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Highly efficient homology-directed repair using transient CRISPR/Cpf1-geminiviral replicon in tomatobioRxiv - Molecular Biology 2019Quote: ... 2015) (Supplemental Table 6 and 7) junctions and a high-fidelity Taq DNA polymerase (Phusion Taq, Thermo Fisher Scientific, USA) and Sanger sequencing (Solgent ...
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bioRxiv - Genetics 2021Quote: ... the PCR products from either primer pairs 6/7 or 9/10 with the plasmid vector pCR 2.1 (Thermo Fisher) followed by transformation of One Shot Top 10 chemically competent cells (Thermo Fisher ...
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bioRxiv - Physiology 2023Quote: ... at 1,000g for 2min and then immediately loaded into the QuantStudio 6 and 7 Flex real-time PCR system (ThermoFisher Scientific). A two-step cycling protocol was implemented to collect cycle threshold (Ct ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then washed and stained for 20 min at 4 °C with the following antibodies: anti-mouse CD8α APC-efluo780 (clone 53-6-7, ebioscience/ Thermofisher), anti-mouse CD8β AF700 or BUV495 (clone YTS156 ...
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bioRxiv - Cell Biology 2023Quote: The ROS dye assay was performed using Di(Acetoxymethyl Ester) (6-Carboxy-2’,7’-Dichlorodihydrofluorescein Diacetate) ROS dye (Invitrogen, D23844), a fluorogenic dye that is converted to 6-CarboxyFluorescein ...