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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 6 IODO BENZO D 1 3 OXAZIN 4 ONE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... they were incubated with donkey anti-chicken rhodamine and donkey anti-rabbit Alexa 488 secondary antibodies for 1.5 hours and then with 1 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen Life Technologies) (1:20 ...
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bioRxiv - Cell Biology 2021Quote: ... 2X SSC) for 5 min and counter stained with 1 μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies). Coverslips were mounted in imaging buffer (3.7 μg/μl glucose oxidase and 1U catalase in equilibration buffer ...
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bioRxiv - Cell Biology 2021Quote: ... the cells were washed three times with PBS and nuclei counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI) (#D3571, Invitrogen) in PBS for 30 min at room temperature ...
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bioRxiv - Neuroscience 2022Quote: ... Secondary antibodies were applied in incubation buffer (1:500 in PBS/BSA) with 4‘,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Doublets and dead cells were excluded based on forward scatter (FSC) and side scatter (SSC) and 4′,6-diamidino-2-phenylindole staining (DAPI, 1 µg/ml; ThermoFisher). All depicted flow cytometry plots were pre-gated on non-debris (by FSC and SSC) ...
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bioRxiv - Microbiology 2021Quote: ... the cells were counterstained in 1 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific GmbH, Bremen, Germany) for 10 min at 46°C ...
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bioRxiv - Microbiology 2022Quote: ... Enteroids were then washed with PBS three times with the addition of 4’,6-diamidino-2-phenylindole (DAPI) nuclear stain (1:5000) (Invitrogen) for the final 10 min wash ...
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bioRxiv - Molecular Biology 2023Quote: ... Slides were then washed and stained with 1:10,000 DAPI (4=,6-diamidino-2-phenylindole, Thermo Fisher Scientific; catalog #: D3571) for 15 minutes at room temperature before coverslips were applied using PBS + glycerol as mounting medium.
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bioRxiv - Cancer Biology 2023Quote: ... and incubated with 0.1% Triton X-100 in PBS supplemented with 4′,6-diamidino-2-phenylindole (DAPI) (1 μg/ml) (Invitrogen, # 62248) for 30 min ...
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bioRxiv - Cell Biology 2024Quote: ... both at 1:300 dilution and mounted with ProLong Gold antifade reagent with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen). Confocal microscopy (Olympus FLUOVIEW FV3000 ...
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bioRxiv - Cell Biology 2019Quote: ... HEK293t were replated every 3 days at a split ratio of 1 in 6 following dissociation with Trypsin 0.05% EDTA (Gibco, Life technologies).
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bioRxiv - Neuroscience 2021Quote: ... and immersed in reagent-2 (diluted 1:2 in PBS) for 6-24 h before incubated in reagent-2 containing TO-PRO-3 (1:5,000, Thermo Fisher Scientific) for additional 7-10 days ...
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bioRxiv - Biochemistry 2023Quote: ... 0.1 μM 6-JOE-conjugated reverse primer (5’-6-JOE-GATGATCTCCACCTTGCCGT-3’) was extended with 1 pmol of RNA as template using Superscript III (Thermo Fisher) as reverse transcriptase ...
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bioRxiv - Immunology 2021Quote: ... working solution in 4% FBS/D-PBS (Gibco/Thermo Fisher Scientific, Germany) was added to each sample to reach a final concentration of 25 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... working solution in 4% FBS/D-PBS (Gibco/Thermo Fisher Scientific, Germany) was added to each sample to reach a final concentration of 25 ng/ml ...
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bioRxiv - Developmental Biology 2022Quote: ... and incubated with Alexa-Flour-488 or 555 secondary antibodies (1:1000) along with 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, Camarillo, CA, 1:1000) for 1 hr at room temperature (RT) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... and rinsed 3 times with Dulbecco’s phosphate buffer saline (D-PBS) (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2023Quote: ... and siTACC3#3: D-004155-04-0005) using Lipofectamine 2000TM (Invitrogen, CA, USA) transfection reagent as described previously24 ...
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bioRxiv - Cancer Biology 2019Quote: ... followed by 3–4-hour incubation at 4°C with protein A/G agarose (20421, Invitrogen). The beads were then collected for western blot detection ...
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bioRxiv - Biophysics 2023Quote: ... 150 mM NaCl) containing N-[4-(7-diethylamino-4-methyl-3-coumarinyl)phenyl]maleimide (CPM; Invitrogen) at a final concentration of 10 μM and incubated for 30 min in the dark on ice ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated overnight in mouse anti-Hu primary antibody at 4°C (1:200 in 3% block; Invitrogen). After three 5-min PB rinses at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... 4) counterstained with the nuclear dye TO-PRO-3 (diluted 1:10’000; Life Technologies, Inc., Gaithersburg, MD, USA). Finally ...
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bioRxiv - Microbiology 2021Quote: ... Paris) and pCMV-VSV-G at a ratio of 4:3:1 with Lipofectamine 3,000 (Thermo Fisher Scientific). Supernatants were collected 48 h after transfection ...
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bioRxiv - Physiology 2022Quote: ... The samples were combined with 1 ml of 3:13 dilution of Erlich’s reagent [1.5 g of 4- (dimethylamino) benzaldehyde (ThermoFisher); 5 ml ethanol ...
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bioRxiv - Biochemistry 2021Quote: EB1 was amplified from pET24d-His-TEV-EB1 plasmid using the primers 5’-CACCATGGCTGTAAACGTCTACTC-3’ and 5’-TTACTTGTAGAGCTCGTCCATGC-3’ and inserted into pENTR/D-TOPO (Invitrogen). Using Gateway LR Clonase II (Invitrogen) ...
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bioRxiv - Neuroscience 2022Quote: Full length mouse Unkempt was amplified from cDNA using primers 5’-CACCAGATATCCAATGTCGAAGGGCCCCGGGCCCG-3’ and 5’-GACGACTCTAGATCACGACTGGAGGGCATGGGCCC-3’ and cloned into pENTR/D-TOPO (ThermoFisher) according to the manufacturer’s instructions to create pENTR-Unk ...
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bioRxiv - Plant Biology 2020Quote: ... obtained from the Arabidopsis Biological Resource Center (ABRC) using primers 5’-caccatggttgtttcaatggctttgg-3’ and 5’-atttgagagagggtcgaaggag-3’ and cloned into pENTR/D-TOPO (Invitrogen). The final construct ...
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bioRxiv - Plant Biology 2020Quote: ... obtained from the Arabidopsis Biological Resource Center (ABRC) using primers 5’-cacccactttctcttttgttagattctagttg-3’ and 5’-cattctataaat-tgattctcctcttctcc-3’ and cloned into pENTR/D-TOPO (Invitrogen). The construct was cloned into pGWB533 (Nakagawa et al. ...
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bioRxiv - Cell Biology 2021Quote: ... The Stim coding sequence was cloned by PCR using primers 5’-CAC CAT GCG AAA GAA TAC CAT TTG GAA C-3’ and 5’-TTC CGT GGC AAG CAG CGA AAA GTT C-3’ and ligated into pENTR/D-TOPO (Invitrogen). Site-directed mutagenesis (Stratagene QuikChange XL ...
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bioRxiv - Genomics 2023Quote: ... HuC/D (mouse, 1:200, Invitrogen), SOX1 (goat ...
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bioRxiv - Neuroscience 2023Quote: ... HuC/D (mouse, 1:200, Invitrogen), KI67 (rabbit ...
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bioRxiv - Genetics 2022Quote: ... 1 nM D-biotin (Invitrogen #B20656) and 2% raffinose.
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bioRxiv - Developmental Biology 2021Quote: ... and further cultured in the DMEM/F12-based medium (D-MEM/F12 supplemented with 4% KSR additive, 1% ITS-X (Gibco), 0.3 % BSA (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: Total RNA was prepared from one lung lobe collected at 3 dpi using lysing matrix D (MP Biomedical) containing 1 ml of TRIzol reagent (Ambion, Life technologies) and homogenization at 20 s at 4.0 m/s twice using MP Biomedical Fastprep 24 Tissue Homogenizer ...
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bioRxiv - Microbiology 2020Quote: ... Approximately 3 kb RT-PCR products covering the S gene deletion were amplified from the viral RNA using the gene specific primers F9newF and F9newR (5’-TAAGGTTGGTGGTAATTATAATTACCTG-3’ and 5’-AAAATAGTTGGCATCATAAAGTAATGGG-3’) and a SuperScript™ IV One-Step RT-PCR System (Invitrogen™, ThemoFisher). A region spanning the deletion was sequenced using primers Wu_24_L and Wu_24_R (5’-TTGAACTTCTACATGCACCAGC-3’ and 5’-CCAGAAGTGATTGTACCCGC-3’).
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bioRxiv - Cancer Biology 2024Quote: ... TRACER rolls and unrolled control samples were incubated at 37 °C in complete organoid media with 50 μM of 5-Iodo-2’deoxyuridine (IdU, Fisher Scientific) and 20 uM of Telox64 (courtesy of Mark Nitz ...
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bioRxiv - Cell Biology 2021Quote: ... Cell pellets were then resuspended and plated into one well of a 6 well plate (Fisher Scientific 1483211). Cells were left to sit for 5 days at 37°C and 5% CO2 after initial plating and were only fed once after three days by adding an additional volume of FCM ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were seeded on one well of a 6-well plate coated with poly-Ornithine/Laminin (Thermo Scientific). Medium was changed every other day ...
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bioRxiv - Cell Biology 2022Quote: ... DNP (EM 1:6-1:30, #71-3500, Invitrogen), DPP4 (IF/FACS 1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP ...
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bioRxiv - Cell Biology 2020Quote: ... One portion was incubated for 16 h at 4°C with Dynabeads (Thermo Fisher Scientific) directly conjugated to anti-CD9 antibody with rotation ...
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bioRxiv - Cancer Biology 2022Quote: ... proteins were separated by one-dimensional gel electrophoresis (4–12% NuPAGE Bis-Tris Gel; Invitrogen), and the entire lane of a Coomassie blue-stained gel was cut into 23 slices ...
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bioRxiv - Molecular Biology 2019Quote: ... One fourth of the eluted samples was separated on 4-12% NuPage gels (Life Technologies) and the gels stained with coomassie blue (ProtoBlue ...
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bioRxiv - Neuroscience 2022Quote: Total RNA was extracted and quantified from WwoxWT/WT and WwoxP47T/P47T n=6 mice/group (3 males and 3 females) and cDNA was synthesized using High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: Tissue cultures (n=3) were transferred as one sample into 250 μl RNAlater (ThermoFisher, Cat# AM7020) and stored at −20 °C ...
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bioRxiv - Molecular Biology 2024Quote: ... one-step RT-qPCR was performed using a QuantStudio 3 Real-Time PCR System (Applied Biosystems) using the Luna Universal One-Step RT-qPCR Kit (NEB) ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Molecular Biology 2021Quote: ... Nuclei were detected by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Carlsbad, CA).
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bioRxiv - Molecular Biology 2020Quote: ... and nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific) at 1:2,000 dilution at RT for 5 min ...
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bioRxiv - Developmental Biology 2022Quote: ... All samples were counterstained in 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, D1306) for 10 minutes at room-temperature and mounted in ProLong Gold Antifade (Thermo fisher Scientific ...