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Citations for Thermo Fisher :
301 - 350 of 10000+ citations for 6 FLUOROQUINOLINE 2 3 DICARBOXYLIC ACID DIETHYL ESTER since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Nuclei were counterstained with Hoechst 33342 DNA dye NucBlue® Live ReadyProbes® reagent for live cell imaging or 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI) and SYTO® 60 fluorescent nucleic acid stain for fixed samples (Invitrogen, Molecular Probes, Germany).
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bioRxiv - Microbiology 2020Quote: ... 6-carboxyfluorescein (FAM)-5= CCG TCA ATC AAG GAG CGC CTC 3=-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Microbiology 2021Quote: ... 6-carboxyfluorescein (FAM)-5’ CCG TCA ATC AAG GAG CGC CTC 3’-6 carboxytetramethylrhodamine (TAMRA) (Life Technologies).Viral RNA was detected using the CFX96 detection system (Bio-Rad Laboratories ...
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bioRxiv - Cell Biology 2021Quote: ... A 10 μl aliquot of Spike S1 at 1 mg/ml as provided by the manufacturer was incubated for 20 min at r.t in the presence of a 10-fold molar excess of FITC or a 7-molar excess of Alexa Fluor 488 carboxylic acid succinimidyl ester (CASE) dye (ThermoFisher Scientific). Afterwards ...
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bioRxiv - Neuroscience 2020Quote: ... was added to 1 mg (1.25 μmol, MW ~ 800) of the Alexa-Fluor®-647 carboxylic acid succinimidyl ester (Invitrogen/ThermoFisher Scientific). The reaction mixture was stirred for 5 h at room temperature in the dark ...
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bioRxiv - Biophysics 2020Quote: ... Pab1 RRM12 and Pub1 RRM123 proteins were purified as described above and were labelled with Alexa Fluor 488 or Alexa Fluor 647 carboxylic acid succinimidyl ester dyes (Molecular Probes), using protein to probe ratios of 1:3 ...
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bioRxiv - Immunology 2023Quote: ... The 14.4.4 mAb and the I-Ak-reactive 11-5.2 mAb were randomly labeled via available lysine residues with Alexa Fluor 647 carboxylic acid succinimidyl ester (Thermo Fisher Scientific) according to the manufacturer’s instructions and purified via gel filtration (Superdex-200 10/300 GL ...
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bioRxiv - Biochemistry 2023Quote: INIP and the TRR complex were labeled on N-termini using AF488 dye (Alexa Fluor 488 carboxylic acid succinimidyl ester, Thermo Fisher). Before labeling ...
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bioRxiv - Immunology 2023Quote: 4CMenB OMVs were fluorescently labelled targeting lysine residues with Alexa Fluor 488 (Alexa Fluor 488 carboxylic acid, succinimidyl ester, Invitrogen, A20000). OMVs were concentrated to 20 mg/ml in PBS 1× pH 7.2 ...
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bioRxiv - Microbiology 2022Quote: ... Roughly 1/3 of the plate (2-3 loopfuls) were added to 400 µl 1x Tris-Ethylenediaminetetraacetic acid (EDTA) pH 7.5 (Thermo Scientific, Waltham, USA) and heated at 80 ºC for 30 min ...
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bioRxiv - Biochemistry 2023Quote: Sample digests were acidified with formic acid to a pH of 2-3 prior to desalting using C18 solid phase extraction plates (SOLA, Thermo Fisher Scientific). Desalted peptides were dried in a vacuum-centrifuged and reconstituted in 0.1% formic acid for LC-MS analysis.
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bioRxiv - Neuroscience 2023Quote: ... and passaged every 4-6 days with 0.5mM ethylenediaminetetraacetic acid (EDTA, Invitrogen 15575020) in Dulbecco’s Phosphate-Buffered Saline (DPBS ...
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bioRxiv - Immunology 2022Quote: ... 3 µl of Dynabeads MyOne Carboxylic Acid (1 μm; ThermoFisher Scientific) were added at a concentration of 0.5 mg/ml to each of the samples ...
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bioRxiv - Molecular Biology 2022Quote: ... Indole-3-acetic acid (IAA) was purchased from ThermoFisher (Product #I3750). IAA treatment was performed as previously described (Zhang et al ...
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bioRxiv - Biophysics 2023Quote: ... 3-(N-morpholino)propanesulfonic acid) (MOPS) was purchased from Acros Organics. Texas Red DHPE (TR-DHPE) ...
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bioRxiv - Biophysics 2022Quote: ... and 2 ng/mL recombinant murine interleukin-3 (IL-3) (Gibco). Cells were grown in a humidified incubator at 37 °C and 6% atmospheric CO2 ...
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bioRxiv - Microbiology 2021Quote: ... Gibco Hepes (N-2-hydroxyethylpiperazine-N-2-ethane sulfonic acid, 0.05 mM; Invitrogen), ROCK Inhibitor Y-27632 (1 mM ...
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bioRxiv - Neuroscience 2023Quote: ... 5mM N-2-hydroxyethyl piperazine-N-2-ethane sulfonic acid (HEPES; Gibco, 15630080), and cOmplete ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were washed 3 times in PBST and mounted in 4′,6-diamidino-2-phenylindole (DAPI)-containing ProLong Anti-fade Diamond mountant (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2020Quote: ... The samples were washed again in PBS for 3×15 min and mounted in DAPI (4-,6-diamidino-2-phenylindole; Life Technologies, D1306) for 5 min ...
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bioRxiv - Neuroscience 2022Quote: ... were loaded at 3 μl/min for 6 min onto a 2 cm × 75 μm C18 trap column (Acclaim Pepmap 100, 3 μm, 300 Å, Thermo Scientific) in loading buffer (0.5% v/v formic acid ...
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bioRxiv - Molecular Biology 2023Quote: ... Biotinylation of Htz1(V126C) was carried out using N-[6-(Biotinamido)hexyl]-3’-(2’-pyridyldithio)propionamide (HPDP-Biotin) (Thermo Fisher cat. # 21341), which has a pyridyl disulfide moiety ...
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bioRxiv - Biochemistry 2022Quote: ICabs A4 and B7 were labelled with NHS-Rhodamine (5/6-carboxy-tetramethyl-rhodamine succinimidyl ester, ThermoFisher Scientific (catalogue no. 46406)) as described by the manufacturer ...
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bioRxiv - Biophysics 2022Quote: ... BODIPY FL NHS Ester (Succinimidyl Ester) (NHS-BODIPY; catalog number: D2184) were purchased from Invitrogen.
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bioRxiv - Immunology 2021Quote: ... + 2 mM Glutamax + 1x non-essential amino acids (Gibco) + 57µM β-Mercaptoethanol (Sigma ...
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bioRxiv - Bioengineering 2022Quote: ... 5,5’-dithiobis-(2-nitrobenzoic acid) (Ellman’s reagent, Life Technologies), pyridine (Fisher) ...
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bioRxiv - Cell Biology 2019Quote: ... supplemented with 2% B-27 without retinoic acid (Gibco) and 1% penicillin/streptomycin (HyClone) ...
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bioRxiv - Cell Biology 2021Quote: ... 10mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES, Gibco), 1% Penicillin/Streptomycin/L-Glutamate (P/S/G ...
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bioRxiv - Microbiology 2020Quote: ... 10 mM 5,5’-dithiobis (2-nitrobenzoic acid) (Thermo Scientific) prepared in 40 mM potassium phosphate buffer (pH 7.5 ...
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bioRxiv - Microbiology 2023Quote: ... 2 mM ethylenediaminetetraacetic acid (EDTA, Fisher Scientific; cat# AM9260G), and 0.5% (v/v ...
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bioRxiv - Neuroscience 2024Quote: ... 2 mL of non-essential amino acids (NEAA) (Gibco), and 400 uL of beta-mercaptoethanol (Life Technologies).
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bioRxiv - Cell Biology 2022Quote: ... A 1 ml aliquot was treated with 2 μM tetramethylrhodamine methyl ester perchlorate (TMRE) (Molecular Probes, USA) and incubated at 37°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... burkhardae cells were stained 2 min at RT with 40 μg/mL AF488 NHS ester (ThermoFisher, Germany). Prior to mounting and STED microscopy ...
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bioRxiv - Microbiology 2021Quote: ... or 10 μM 7-hydroxy-9H-(1,3-dichloro-9,9-dimethylacridin-2-one) succinimidyl ester (DDAO-SE) (Invitrogen) in HMI11 and incubated for 20 min at 37°C ...
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bioRxiv - Cell Biology 2023Quote: ... Mitochondria labeling was achieved by adding 2 µL of a tetramethyl rhodamine methyl ester (TMRM, ThermoFisher, T668) directly to the medium to achieve a final concentration of TMRM of 100 nM ...
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bioRxiv - Cell Biology 2020Quote: ... and 2 mg/ml 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher Scientific, 11916621) for 1 hour ...
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bioRxiv - Microbiology 2021Quote: ... adding 2 μg/mL DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific) and 1 μM QUMA-1 23 to the final wash ...
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bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
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bioRxiv - Cell Biology 2020Quote: ... or BODIPY™ 558/568 C12 (4,4-Difluoro-5-(2-Thienyl)-4-Bora-3a,4a-Diaza-s-Indacene-3-Dodecanoic Acid) (Thermo Fisher Scientific, Waltham, MA).
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bioRxiv - Cell Biology 2020Quote: Fibroblasts were plated in 96-well plates (10,000 fibroblasts/well) for 3 days in 2% FBS in DMEM supplemented with ascorbic acid (50 μg/mL) (Fisher Scientific, Waltham, MA, USA). Fibroblasts and matrix were then fixed and immunostained as detailed above ...
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bioRxiv - Neuroscience 2020Quote: ... was added to 1 mg (1.25 μmol, MW ~ 800) of the Alexa-Fluor®-647 carboxylic acid succinimidyl ester (Invitrogen/ThermoFisher Scientific). The reaction mixture was stirred for 5 h at room temperature in the dark ...
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bioRxiv - Microbiology 2023Quote: Cell pellets were acid digested with 2 mL of Optima grade nitric acid (ThermoFisher, Waltham, MA) and 500 μL hydrogen peroxide (Sigma ...
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bioRxiv - Developmental Biology 2020Quote: Islet cell clusters were incubated (25 min at 37 °C) in RPMI supplemented with 2 μM Fura-2 acetoxymethyl ester (Molecular Probes, Eugene, OR). Fluorescence imaging was performed using a Nikon Eclipse TE2000-U microscope equipped with an epifluorescence illuminator (SUTTER ...
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bioRxiv - Neuroscience 2022Quote: ... with 16 μM BSA in the presence of CaCl2 (1.3mM) and fura-2-acetoxymethyl ester (fura 2-AM, 5 μM: Molecular Probes, Eugene, OR, USA), and they were incubated at 37 °C for 25 min ...
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bioRxiv - Neuroscience 2023Quote: ... The cells were loaded with 5 μM acetoxymethyl-ester of Fura-2 (Fura-2/AM) and 80 μg /ml pluronic F127 (Molecular Probes, Eugene, Oregon) for at least 30 minutes at room temperature ...
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bioRxiv - Biophysics 2021Quote: ... 20 μL of Alexa fluor 488 NHS ester (or succinimidyl ester) (ThermoFisher Scientific, product number-A20000) (5mg/ml ...
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bioRxiv - Bioengineering 2023Quote: ... The washed PEG-free bundles were labeled by Alexa Fluor 647 NHS Ester (Succinimidyl Ester) (Invitrogen) diluted in PBS at the ratio of 1:1000 and incubated at 37°C for 30 minutes ...
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bioRxiv - Biophysics 2023Quote: ... and Alexa Fluor™ 488 NHS Ester (Succinimidyl Ester) were purchased from Thermo Fisher (Watham, MA). [Leu5]-enkephalin (YGGFL) ...
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bioRxiv - Biophysics 2024Quote: ... The resulting pellet was used for labelling with Alexa Fluor 647 NHS ester (succinimidyl ester, Invitrogen) in 100 mM sodium bicarbonate buffer pH 8.3 ...
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bioRxiv - Developmental Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies #D1306) was used for nuclei staining in conjunction with secondary antibodies ...