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Citations for Thermo Fisher :
451 - 500 of 10000+ citations for 6 Chloro 9 fluorobenz 9 isoquinoline 5 10 dione since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2019Quote: ... were sorted directly on a FACS Aria III in 9 μl of Specific Target Amplification (STA) reaction mix from the CellsDirect One-Step qRT-PCR kit (Invitrogen) containing 0.2XTaqMan Gene Expression Assay mix ...
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Neuronal L-Type Calcium Channel Signaling to the Nucleus Requires a Novel CaMKIIα-Shank3 InteractionbioRxiv - Neuroscience 2019Quote: ... Neurons were transfected at 7-9 days in vitro (DIV) using Lipofectamine 2000 following the manufacturer’s directions (Thermo Fisher Scientific). A total of 1 μg of DNA was transfected for each well of a 12-well plate for 2-3 hours before switching back to conditioned media ...
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bioRxiv - Immunology 2021Quote: ... Cells were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... were transfected with 9 μg of the appropriate hairpin in a pLKO-backbone vector using 30 μl of Lipofectamine 2000 (Invitrogen). After 24 hours ...
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bioRxiv - Neuroscience 2021Quote: ... and gene specific primer pairs (Supplementary Table 9) on a QuantStudio™ 7 Flex Real-Time PCR System (Applied Biosystems). Relative mRNA expression levels were calculated using the 2−ΔΔCt method and RPL18A as the house-keeping gene.
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bioRxiv - Bioengineering 2020Quote: ... 1 mg of fusion protein was dialyzed in 0.1 M NaCO3 pH 9 then mixed with 25 μg of FITC (ACROS organics) in a total volume of 0.5 mL ...
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bioRxiv - Cancer Biology 2020Quote: 293FT were used to prepare all lentiviruses and retroviruses and were maintained at 37°C in 9% CO2-containing humidified incubators using DMEM (Gibco) containing 10% fetal bovine serum (Hyclone ...
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bioRxiv - Microbiology 2020Quote: ... T4 phage were added (3×108 phages ml-1) to the AFM bio-cell together with SYTO 9 and PI (BacLight Bacterial Viability and Counting Kit, Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2021Quote: ... and fragmented for 9 min at 94°C in 2X Superscript III first-strand buffer containing 10mM DTT (Invitrogen, #P2325). The 10 μl of fragmented RNA was added to 0.5 μl of Random primers (Invitrogen ...
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bioRxiv - Cell Biology 2021Quote: ... Flp-In-293 cells were transfected with a 9:1 ratio of pOG44:pcDNA5/FRT-PRPF4 plasmids using Lipofectamine 2000 (Invitrogen) and 48 h post-transfection ...
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bioRxiv - Neuroscience 2022Quote: ... from each sample was labeled with 9 different TMT-10plex tags accordingly to the manufacturer’s protocols (cat# 90110, Thermo Scientific). The same mass tag and sample assignment was maintained throughout the entire study (old brain samples were labeled with TMT10-126 ...
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bioRxiv - Plant Biology 2022Quote: ... 9 RNA samples were extracted using TRIzol(tm) and RNA Purification Kit following the manufacturer’s protocol (ThermoFisher, Waltham, MA, USA). RNA was further purified using the TURBO DNA-free(tm ...
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bioRxiv - Neuroscience 2022Quote: ... was dissolved in 0.9% sterile Saline and pH was adjusted to 7.4 with 20-30 µl 1 N NaOH (#SS266-1, Fisher Scientific) according to Tocris Bioscience’s guidelines ...
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bioRxiv - Cancer Biology 2022Quote: ... RNA was isolated from 9 µl lysate and 1 µl of ERCC 1:1000 Spike in control (Thermo Fisher Scientific) per sample using RNA SPRI beads (Agencourt ...
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bioRxiv - Biochemistry 2022Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). Plasmids were transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Microbiology 2020Quote: ... Israel) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Molecular Biology 2020Quote: ... and pcDNA5/FRT/TO-based plasmids carrying the GFP-RIF1-L or GFP-RIF1-L-pp1bs gene were mixed in 9:1 molar ratio and used for transfection of Flp-In T-Rex 293 cells (Invitrogen) with Lipofectamine 3000 (Invitrogen) ...
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bioRxiv - Genetics 2020Quote: TaqMan copy number assays for CYP2D6 (assay IDs Hs04083572_cn and Hs00010001_cn for intron 2 and exon 9 respectively, Thermo Fisher Scientific) were run according to the manufacturer’s protocol on a ViiA7 Real-Time PCR System (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2022Quote: ... 9 μL of total RNA samples were proceeded to cDNA synthesis using High Capacity cDNA Reverse Transcription kit (Applied Biosystems). Then ...
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bioRxiv - Immunology 2022Quote: Human bronchial epithelial cells (BEAS-2B cells, ATCC® CRL-9609™) were cultured in LHC-9 medium (Life Technologies) and human lung fibroblast (MRC-5 cells ...
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bioRxiv - Cancer Biology 2022Quote: ... cells were cultured in DMEM with 10% FBS and 1% P/S and were bulked up to 9 x T175 flasks of each by splitting and reseeding during their growth phase (∼80% confluence) using TrypLE (Gibco). The 9 x T175 flasks were seeded at ∼30% confluence in serum-containing media ...
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bioRxiv - Biochemistry 2022Quote: ... Baculoviruses for expression of the DNMT1 were generated in Spodoptera frugiperda 9 (Sf9) cells according to the Bac-to-Bac system instruction (Invitrogen). The protein expression was performed by infection of the baculoviruses with the Sf9 cells for 72 hrs at 27 °C ...
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bioRxiv - Pathology 2020Quote: ... 6-dihydroxy-2,4,5,7-tetraio-dospiro (isobenzofuran-1(3H),9[9H] xanthan)-3:1 dipotassium salt (50 mg/kg in 0.9% saline, Fisher Scientific) was injected retro-orbitally before catalyzing vessel injury with a 540 nm laser ...
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bioRxiv - Cell Biology 2019Quote: ... the dishes were coated with 1 ml of gelatin in PBS (1: 9 of 0.1% fluorescein isothiocyante (FITC)-gelatin (# G13186, Invitrogen, MA): 2% porcine gelatin) ...
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bioRxiv - Systems Biology 2021Quote: ... day 9 spheroids were dissociated and stained for 30 minutes with Image-iT Lipid Peroxidation Sensor C11-BODIPY (Thermo Fisher) at a final concentration of 10 μM according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2020Quote: ... Per2Luc mice and littermate wild-type control mice were euthanized by isoflurane 2-3 hours before lights off (ZT 9–ZT10) and tissues were immediately removed into ice-cold HBSS (Gibco). Coronal brain slices containing SCN (400-µm thickness ...
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bioRxiv - Immunology 2020Quote: ... TIB-47) were maintained at 3×105 to 9×105 cells/ml in Roswell Park Memorial Institute medium (RPMI GlutaMAX, Gibco) supplemented with 10% (vol/vol ...
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bioRxiv - Microbiology 2021Quote: ... 20 µg of total RNA was reverse transcribed using 9 ng of oligo(dT)21 and 15 ng of random octamers (Invitrogen) in the presence of Cy3 or Cy5-dCTP (Invitrogen ...
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bioRxiv - Synthetic Biology 2020Quote: ... transfection complexes containing 3 μL of Xtremegene-9 reagent and 500 ng of plasmid DNA were prepared in 100 μL of OptiMEM (Invitrogen). After 20 min of incubation at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... Plasmids were transfected into cultured neurons at DIV 7-9 using a calcium phosphate transfection kit (Thermo Fisher, K2780-01). Experiments were performed 2 or 3 days post transfection.
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bioRxiv - Microbiology 2020Quote: ... CLR-1586) cells were cultured at 37°C in 9% CO2 in Dulbecco’s modified Eagle’s medium (DMEM, Gibco™, ThemoFisher) supplemented with 5% fetal calf serum (FCS ...
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bioRxiv - Microbiology 2022Quote: ... Liquid cultures of Mtb were grown at 37 °C with shaking in Middlebrook 7H9 (Thermo Fisher Scientific, DF0713-17-9) broth supplemented with 0.02 % tyloxapol and albumin (Millipore Sigma ...
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bioRxiv - Developmental Biology 2022Quote: ... Selection of and enrichment for cardiomyocytes was initiated on day 9 by culturing cells for 4 days in RPMI 1640 medium without glucose (Gibco) supplemented with 1x CDM3 supplement (Sigma Aldrich ...
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bioRxiv - Cancer Biology 2022Quote: Libraries were amplified with 9 cycles of PCR reactions and quantified using Qubit dsDNA HS Assay Kit (Thermo Fisher Scientific). Libraries were sequenced on NovaSeq 6000 (Illumina ...
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bioRxiv - Immunology 2022Quote: ... stimulation for analysis of cytokine production using a Luminex-based multiplexed assay (Th1/2/9/17 18-plex Human ProcartaPlex Panel, ThermoFisher) per manufacturer recommendation ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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SLC15A4 favors inflammasome function via mTORC1 signaling and autophagy restraint in dendritic cellsbioRxiv - Immunology 2022Quote: Bone marrow cells were isolated and cultured for 7-9 d in RPMI-1640 medium (Gibco, ThermoFisher Scientific, Waltham, MA) supplemented with 10% low endotoxin-FBS (Hyclone ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Mouse glucagon-releasing cell line αTC1-9 (RRID: CVCL_0150, ATCC, Manassas, VA, USA) was cultured in DMEM (Invitrogen, Barcelona, Spain) without phenol red supplemented with 2 mM l-glutamine ...
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bioRxiv - Neuroscience 2022Quote: ... obtained from a previous stock of SIP (9 parts of Percoll per 1 part of PBS 10X (GIBCO, 14200-067)) ...
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bioRxiv - Neuroscience 2022Quote: ... A 11.2 kb PCR product that included a region from CLN3 exon 3 to exon 9 was amplified using a high fidelity polymerase (Platinum Taq High Fidelity, Invitrogen) and CLN3 primers pCLN3F3 and pCLN3R3 (Supplemental Table 2) ...
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bioRxiv - Molecular Biology 2022Quote: ... 5μl of each of the three 9-LOX amplicons were mixed with 1μl of 6X gel loading dye (Thermo-Fisher Scientific), loaded on a 1% agarose gel and electrophoresed against a 1kb DNA ladder (RTU-Thermo Scientific ...
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bioRxiv - Microbiology 2023Quote: ... after vaccination and boost (13 weeks after the beginning of the experiment or prime vaccination/ 9 weeks after Prime and Boost vaccination) were diluted in Opti-MEM media (Thermofisher) in serial 3-fold dilutions ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in FreeStyle 293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Turbo293 (SpeedBiosystems ...
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bioRxiv - Molecular Biology 2023Quote: ... human kidney) were maintained at 37°C and 9% CO2 in a 75% humidified atmosphere in Expi293 Expression Medium (GIBCO). The Plasmid was transiently transfected using Expifectamine 293 (GIBCO ...
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bioRxiv - Biochemistry 2023Quote: ... inside of a GC vial with a 9 mm PTFE/red rubber septum (CAS#C4000-30, Thermo scientific, Rockwood, TN). Samples were placed in an AI/AS 1300 autosampler prior to auto injection ...
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bioRxiv - Neuroscience 2023Quote: ... we conducted control experiments in WT mice by performing AAV2/9-hSyn-Flex-Synaptophysin-mRuby injection mixed with CTB-647 (C34778, ThermoFisher) into the IC ...
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bioRxiv - Cancer Biology 2023Quote: ... and the lower surface of the insert was stained with 0.5% crystal fast violet (548-62-9, Acros organics, USA). The cells were counted and captured under a Nikon Eclipse 80i microscope at 10× magnification.
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bioRxiv - Genomics 2023Quote: ... 9 ng of cDNA were used as template and reactions were performed using Power SYBR Green master mix (Applied Biosystems) on a StepOne Plus Real-Time PCR machine ...
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bioRxiv - Bioengineering 2024Quote: ... AAV2/9 viruses were stored at −80°C until use and diluted with phosphate-buffered saline (PBS, Thermo Fisher Scientific) if necessary.
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bioRxiv - Cell Biology 2023Quote: hSHEDs cultured at passage 9 were seeded in 100mm culture dishes at a density of 5000 per cm2 and grown in KnockOut DMEM (Gibco) media supplemented with 10% FBS (Gibco) ...