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Citations for Thermo Fisher :
601 - 650 of 10000+ citations for 6 Chloro 7H pyrrolo 2 3 d pyrimidine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: ... N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM 4-64; 50 μM) (Invitrogen) or propidium iodide (PI ...
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bioRxiv - Neuroscience 2023Quote: ... 6 and 8 weeks of maturation were loaded with 2.5 µM Fluo-3-AM (Molecular Probes, #F1242) dissolved in differentiation medium for 30 min at 37 °C and at 5% CO2 ...
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bioRxiv - Cancer Biology 2019Quote: ... beads were resuspended in SDS loading buffer supplemented with 2 mM D-biotin (Invitrogen).
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bioRxiv - Molecular Biology 2021Quote: Approximately 60 WT and MafAS64F/+ islets from at least 6 female and male mice were analyzed with the ratiometric calcium indicator fura-2-acetoxymethylester (Fura-2 AM) (Life Technologies). Islets were maintained in 5 mM glucose for 30 min prior to measuring 11 mM glucose-induced calcium oscillations and depolarization-activated calcium influx with 30 mM KCl ...
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bioRxiv - Cell Biology 2024Quote: ... the conversion of non-fluorescent 2’,7’-bis-(2- carboxyethyl)-5-(and-6)-carboxyfluorescein acetoxymethyl ester (BCECF AM) (Invitrogen, Waltham, MA) into a pH sensitive fluorescent indicator by the intracellular esterase was used to measure the pH ...
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bioRxiv - Cell Biology 2020Quote: ... incubated 2-3 minutes with SuperSignalTM West Pico Chemiluminiscent Substrate (Thermo Scientific) and imaged using C-DiGit® Blot Scanner (LI-COR).
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bioRxiv - Microbiology 2019Quote: ... and 2 μl of 1 μM ToPro 3 (Molecular Probes T-3605), a nucleic acid dye that only permeates cell membranes of dead cells ...
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bioRxiv - Biochemistry 2020Quote: 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT; Thermo Fisher Scientific) was added to cells at a final concentration of 2mM and incubated at 37°C in 5%CO2 for 1 h ...
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bioRxiv - Biochemistry 2021Quote: ... A predesigned TaqMan assay targeting exon 2–3 boundary (Hs00213726_m1; Life Technologies) was also used to ensure equal amount of the endogenous A4GALT transcript in transfected and non-transfected cells ...
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bioRxiv - Cancer Biology 2020Quote: ... anti-phospho-Pak1-2-3 (pSer141) (44-940G, 1:2000) from Invitrogen/Thermo Fisher Scientific (Carlsbad ...
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bioRxiv - Cell Biology 2021Quote: ... Jade1/2/3 mRNA was assessed by SYBR Green (Thermo Fisher Scientific) qPCR using Hprt1 as endogenous control ...
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bioRxiv - Cell Biology 2021Quote: ... phospho-PAK1/2/3 (Thr402) (ThermoFisher PA1-4636, 1:1000 for WB), phospho-PAK4 (Ser474)/PAK5 (Ser602)/PAK6 (Ser560 ...
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bioRxiv - Cell Biology 2021Quote: ... and were passaged every 2 or 3 days using 0.05% Trypsin (Gibco). mESCs were used at passages below 30 ...
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bioRxiv - Neuroscience 2020Quote: ... Fibroblasts were fed every 2-3 days with DMEM (ThermoFisher Scientific, #11995073) media supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... Cells were passaged once every 2-3 days by trypsinization (Gibco, 25300054) upon reaching ∼70-80% confluency ...
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bioRxiv - Physiology 2020Quote: ... for 3 days with macrophage-conditioned medium containing 2% Horse Serum (Gibco). Cells were washed ...
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bioRxiv - Cell Biology 2019Quote: ... which were activated through consecutive incubation with 2% 3-aminopropyltrimethoxysilane (Acros Organics) in isopropanol for 15 min at room temperature ...
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bioRxiv - Cell Biology 2020Quote: ... spiked with 2-3 μL Plus reagent (15338100, Thermo Fisher Scientific, USA). The plasmid solution was mixed and incubated for 10 min at room temperature ...
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bioRxiv - Pathology 2022Quote: ... 2 and 3 were quantified using CyQUANT Proliferation Assay Kit (Invitrogen, C35011); For myofibroblast differentiation assay ...
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bioRxiv - Molecular Biology 2023Quote: ... Dynabeads with precipitated proteins were washed 3 times with DynaMag-2 (Invitrogen) and eluted with LDS buffer (Invitrogen ...
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bioRxiv - Neuroscience 2022Quote: ... This medium was exchanged 2-3 hours after plating with Neurobasal (Gibco) supplemented with L-glutamine (2 mM) ...
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bioRxiv - Microbiology 2023Quote: ... 5-[3-aminoallyl]-2’-deoxyuridine-5’-triphosphate (aminoallyl-dUTP, Thermo Scientific™) was incorporated into a PCR by the use of a DreamTaqTM DNA Polymerase (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2023Quote: ... 2- & 3-methyl pentane and n-hexane (Thermo Scientific, Waltham, MA, USA). Reported compounds detected by the GC-MS were confirmed by matching retention times and mass–charge (m/z ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were split every 2 to 3 days using TrypLE Express (Gibco).
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bioRxiv - Immunology 2023Quote: ... 3) Dyes: NucBlue Live Cell Stain (2 drops/ml, R37605, Molecular Probes), phalloidin-568 (A12380 ...
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Self-organised pattern formation in the developing neural tube by a temporal relay of BMP signallingbioRxiv - Developmental Biology 2023Quote: Cells were passaged every 2-3 days by incubation with Accutase (Gibco) for 2-3 min at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and TaqMan assay reagents (Table 2) on the QuantStudio 3 (Applied Biosystems). Gene expression was normalized to GAPDH expression ...
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bioRxiv - Plant Biology 2020Quote: ... from cDNA using the primers indicated in Supplemental Table 3 and transferred into pENTR-D-TOPO (Invitrogen) via Gibson assembly using NEB Gibson Assembly Master Mix according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... containing a 5′-CACC-3′ sequence required for directional TOPO cloning in pENTR/D-TOPO (Invitrogen, USA), and a reverse primer [“Tgd057 R HA stop” 5’-TTACGCGTAGTCCGGGACGTCGTACGGGTACTCGACCTCAATGTTGTATTC-3’] ...
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bioRxiv - Developmental Biology 2020Quote: ... The cen-bcd-3’UTR fusion was assembled and directionally cloned into the pENTR-D vector (Invitrogen) by Gibson assembly using 5-fold molar excess of the bcd-3’UTR ...
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bioRxiv - Cell Biology 2021Quote: ... The βarr1/2 siRNA (5’-ACCUGCGCCUUCCGCUAUG-3’) and a scrambled siRNA (control, 5’-UGGUUUACAUGUCGACUAA-3’) (Dharmacon) were transfected by RNAimax (Invitrogen) according to the instructions of the manufacturer ...
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bioRxiv - Synthetic Biology 2024Quote: ... the full volume of media in each well was pipetted gently 4-5 times and added to 1 mL of FACS buffer containing 3 uM DAPI (PBS pH 7.4, 2–5 mM EDTA, 0.1% BSA, 3 uM DAPI (Thermo Scientific #62247)) ...
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bioRxiv - Cell Biology 2020Quote: Live HUVECs and HEK293 cells were stained with Laurdan dye (6-dodecanoyl-2-dimethylaminonaphthalene) (ThermoFisher) at 10 μM (HUVECs ...
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bioRxiv - Neuroscience 2020Quote: ... stained with 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Grand Island, NY, USA) for 15 minutes ...
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bioRxiv - Neuroscience 2021Quote: ... fixed larvae were stained with 2.5 ug/ml 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen) diluted in PBS to label all cell nuclei ...
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bioRxiv - Molecular Biology 2019Quote: ... 6’-Diamidino-2-phenylindole (DAPI; Cat#D1306; RRID: AB_2629482) were purchased from Invitrogen (Carlsbad, CA). Neurobasal® medium ...
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bioRxiv - Immunology 2022Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) and LysoTracker Red DND-99 were purchased from Invitrogen, Carlsbad ...
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bioRxiv - Microbiology 2019Quote: ... nuclei were stained using Prolong gold antifade containing 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen). All preparations were observed with a confocal microscope (ZEISS LSM 700 inverted ...
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bioRxiv - Genomics 2021Quote: ... the cells were counterstained with 4′,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher Scientific, R37606). Cells were then viewed and photographed using a fluorescence microscope (Zeiss ...
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bioRxiv - Cell Biology 2020Quote: ... Mounting media with DAPI (4’,6-diamidino-2-phenylindole) used was either ProLong Gold (Invitrogen) or ProLong Diamond (Invitrogen) ...
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bioRxiv - Bioengineering 2021Quote: ... cell nuclei were stained with DAPI (4’,6-diamidino-2-phenylindole, dilactate; 2μM, Life Technologies). Cells were visualized in PBS with calcium and magnesium (Gibco ...
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bioRxiv - Bioengineering 2020Quote: ... then incubated in 4’,6-diamidino-2-phenylindole (DAPI) (1:1000) (Invitrogen, Carlsbad, CA, USA) for 10 minutes and washed with PBS again ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were counterstained with DAPI (4ʹ,6-diamidino-2-phenylindol) 1:10,000 (Thermo Fisher Scientific). Images were recorded using an Axioimager 2 Microscope with cooled CCD camera (SPOT Imaging Solutions ...
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bioRxiv - Neuroscience 2020Quote: ... Cells were stained with 4′-6-diamidino-2-phenylindole (DAPI; Life Technologies, Carlsbad, CA, USA) before examination under a fluorescence microscope (Zeiss LSM 710).
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bioRxiv - Microbiology 2020Quote: ... cell nuclei were stained with DAPI (4 =,6 =-diamidino-2-phenylindole) (Thermo Fisher Scientific, D1306) and mounted on slides with Prolong diamond antifade mounting medium (Invitrogen ...
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bioRxiv - Microbiology 2019Quote: ... 2 µg DNA and 6 µL PEI were incubated with 50 µL Opti-MEM (Gibco) seperately for 5 minutes before combining and incubating for 20 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The culture medium was replaced with fura-2 AM (6 μM, F1221; Thermo Fisher Scientific) in Opti-MEM (11058-021 ...
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bioRxiv - Neuroscience 2022Quote: ... Nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2ng/ml; Molecular Probes). Acti-stain 555 phalloidin (Cytoskeleton Inc ...
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bioRxiv - Bioengineering 2022Quote: ... Cell nuclei of HUVECs were stained by 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, D1306) at 1:500 dilution for 15 min ...
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bioRxiv - Bioengineering 2022Quote: ... cell nuclei were stained with 1:1000 DAPI (4′,6-Diamidino-2-Phenylindole, Dihydrochloride, ThermoFisher) in PBS ...