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Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 6 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... 6 units of Turbo™ DNase (Invitrogen, USA) and 15 units RNase Ⅰ (Invitrogen ...
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bioRxiv - Bioengineering 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI) (Thermo Fisher) was added for 30 min at room temperature before the secondary antibodies were washed out in PBS 3x for 15 min ...
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bioRxiv - Neuroscience 2024Quote: ... in Essential 6 medium (Thermo Fisher Scientific, A1516401) supplemented with patterning molecules as shown in Supplementary Data Fig ...
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bioRxiv - Biochemistry 2024Quote: ... 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, D3571) was added for 5 minutes in the dark ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-CD11a (1:50, IBL-6/2, Invitrogen), and FITC anti-PD-1 (1:50 ...
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bioRxiv - Genetics 2024Quote: ... 15 μL qPCR (Applied Biosystems, Quantstudio 6 Flex) was then performed to determine the additional number of cycles ...
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bioRxiv - Evolutionary Biology 2024Quote: ... in water in 6-well plates (ThermoFisher Scientific) placed on an orbital shaker moving at slow speed at 27°C until needed ...
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bioRxiv - Genetics 2024Quote: ... qPCR was performed on QuantStudio 6 (Applied Biosystems), using 10 ng of cDNA and 250 nM of each primer on a MicroAmp 384-well reaction plate (Applied Biosystems #4309849) ...
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bioRxiv - Immunology 2024Quote: ... and the Quantstudio 6 Flex System (Applied Biosystems). Kars RNA was reverse-transcribed then amplified using primers specific to an 85 bp region overlapping the junction of exon six and seven in the Kars gene ...
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bioRxiv - Cell Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (Life Technologies #D3571) for 10 min ...
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bioRxiv - Molecular Biology 2024Quote: ... for 6 hours in Opti-MEM (Life Technologies).
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Parkinson’s VPS35[D620N] mutation induces LRRK2 mediated lysosomal association of RILPL1 and TMEM55BbioRxiv - Neuroscience 2023Quote: ... in a 6 well plate (Fisher Scientific, US) at 60% confluency ...
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bioRxiv - Microbiology 2024Quote: ... anti-6*his tag (Thermo Fisher Scientific,11533923), anti-myc tag (Abcam ...
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bioRxiv - Cancer Biology 2023Quote: ... DAPI (4-6-diamindino-2-phenylindole; Molecular Probes) was used to detect DNA ...
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bioRxiv - Microbiology 2022Quote: ... Fluorescence was measured as temperature increased 1°C/minute from 25°C to 95°C in a StepOne Plus qPCR instrument (Applied Biosystems). Protein from 2 independent purifications was used ...
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bioRxiv - Biochemistry 2021Quote: ... The protein was run over either one (at 10°C) or two (at 10°C and 2°C) immobilized pepsin columns (Applied Biosystems; Poroszyme Immobilized Pepsin Cartridge ...
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bioRxiv - Microbiology 2021Quote: ... 96°C for 1min and 25 cycles (10sec at 96°C, 5sec at 50°C and 3min at 60°C) and a 2720 Thermal cycler (Applied Biosystems). For each RT-PCR product ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and the samples were run on a thermocycler (60 min, 37°C; 5 min, 95°C; held, 4°C; Applied Biosystems); cDNA was stored at −20°C until use.
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bioRxiv - Molecular Biology 2022Quote: ... cyt-c-p (1:100, incubated overnight at 4°C, Thermo Fisher Scientific). All biological replicates were tested by western blotting.
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bioRxiv - Physiology 2022Quote: ... C-IV-II and C-V-alpha (ThermoFisher Scientific, 45-8199; 1:2500). Secondary antibodies used were horseradish peroxidase–conjugated anti-mouse (Cell Signaling Technology ...
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bioRxiv - Molecular Biology 2023Quote: ... 60°C for 30 s and 72°C for 30 s (Applied Biosystems). 2−ΔΔCt method algorithm was used to analyze the relative changes in expressions ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Systems Biology 2021Quote: ... The cells were maintained in fibroblast media for 6 days then passaged onto Matrigel-coated dishes for further reprogramming in Essential 6 medium plus human bFGF (ThermoFisher Scientific). The iPSC colonies were manually picked onto Matrigel-coated plates to generate stable iPSC lines ...
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bioRxiv - Developmental Biology 2022Quote: ... embedded in paraffin using the Tissue-Tek VIP 6 (Sakura) tissue processor and of 6 µm sections obtained with a microtome (HM355S, Thermo Scientific). Z-shaped probes for Sema6d (565871) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Systems Biology 2020Quote: 1×10^6 cells were seeded in wells of 6-well plates (FALCON # 353046) in MEM without phenol red (Gibco # 51200038) supplemented with 0.5% Fetal Calf Serum (VWR #89510-184) ...
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bioRxiv - Genomics 2021Quote: ... 600,000 cells were plated in 6 well plates and transfected with 2 μg plasmid DNA using 6 μl Lipofectamine LTX reagent (Thermo Fisher). Medium was exchanged after 16 h and harvested 24 h later ...
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bioRxiv - Physiology 2022Quote: ... Mitochondria membrane polarization was measured by loading cells with 2 µM JC-1 (5, 5’, 6, 6’-tetrachloro-1, 1’, 3, 3’-tetraethylbenzimidazolylcarbocyanine iodide, Invitrogen, 15003) at 37°C for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... cells were plated at 650,000 cells per well of a 6 well plate and infected with 6 dilutions from a 10-fold dilution series in 1% FBS (Gibco, MA)/1X non-essential amino acids (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... The IL-6 levels in plasma were analyzed using a Mouse IL-6 Uncoated ELISA kit (#88-7064, Invitrogen, MA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... At 5-6 dpf each FoxP2.A:FingR(PSD95)+ larva was placed into individual wells of a 6-well plate (Thermo Fisher Scientific) containing approximately 10mL of fish water ...
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bioRxiv - Neuroscience 2019Quote: ... 5µl each from the standards and the rAAV dilutions along with 15µl of mastermix were subjected to qPCR at 95°C 10 min / 95°C 15 sec / 60°C 1 min/ repeat 40×/ melt curve using StepOne machine (Applied Biosystems, USA). Data analyses were performed by StepOne2.3 software and by Microsoft excel.
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bioRxiv - Biochemistry 2021Quote: ... The FL-NP construct included a C-tag peptide (EPEA) at the C-terminus (27) for affinity chromatography purification using a C-tag affinity resin (Thermo Fisher Scientific), eluting with 20 mM Tris-HCl ...
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bioRxiv - Neuroscience 2021Quote: ... This reaction was incubated for 60 minutes in triplicates for each temperature (37°C, 40°C and 42°C) and terminated by adding one volume of sample buffer (NuPAGE LDS; Invitrogen, Carlsbad, CA) containing 5% of 2-β-mercapto-ethanol ...
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bioRxiv - Microbiology 2021Quote: ... [C]-Ahx-QAHAEERLEDIDYVE-acid (Cambridge Research Biochemicals, Billingham, UK) and C-terminal region of FtsZ2: NH2-[C]-SDGGRDEVEKNNGLDVIR-COOH (Thermo Fisher Scientific). H ...
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bioRxiv - Immunology 2021Quote: ... 4°C) and incubated overnight at 4°C with gentle rotation with 40 μl Pierce Anti-c-Myc Magnetic Beads (ThermoFisher Scientific Cat# 88842) or anti-Flag® M2 Magnetic Beads (Merck Cat# M8823 ...
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bioRxiv - Neuroscience 2022Quote: ... sh-c-Jun and c-Jun over-expressed (pCMV-c-Jun) plasmid were transfected into cells using lipofectamine 3000 reagent (Invitrogen; Carlsbad, CA, USA) according to the manufacturer’s recommendation ...
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bioRxiv - Systems Biology 2021Quote: ... The Feed C regime used commercial CD EfficientFeed™ C AGT™ (Invitrogen, UK), whereas the U and U40 feeds aimed to provide growth-limiting nutrients (glucose and amino acids ...
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bioRxiv - Cancer Biology 2019Quote: ... All TaqMan probes were 5′-6-carboxyfluorescein (FAM) and 3′-6-carboxy-N,N,N′,N′-tetramethylrhodamine (TAMRA) labeled (Applied Biosystems, US) except TATA-binding protein (TBP ...
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bioRxiv - Molecular Biology 2020Quote: ... (G4S)6 Linker-VH/CH1 and (G4S)6 Linker-VH/CH1-(G4S)6 Linker-VH/CH1 sequences were synthesized using GeneArt® gene synthesis service (ThermoFisher Scientific).
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bioRxiv - Immunology 2020Quote: ... Serial frozen sections (6 μm in thickness) were cut on a cryostat and counterstained with DAPI (4′,6-Diamidine-2′-phenylindole; Thermo Fisher Scientific). The number of beads in the SED from 3-4 sections of two Peyer’s Patches per mouse (n=3–4 mice/group ...
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bioRxiv - Cell Biology 2020Quote: ... Adult cardiac NMCs were isolated from adult C56BL/6 mice (6-8 weeks old) by digesting adult ventricles in buffer containing 1 mg/ml collagenase IV (Gibco 17104-019) and 1.2 mg/ml dispase II (Sigma ...
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bioRxiv - Genomics 2021Quote: ... cells were seeded in 6-well plates or 6 cm dishes and reverse transfected with 5 nM Silencer Select siRNAs (Thermo Fisher Scientific) using RNAiMAX (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... iPSC cultures at ~90% confluence in 6-well-plates were treated with 6 μM CHIR-99021 (SelleckChem) in RPMI 1640 medium supplemented with B27 supplements (Thermo Fisher Scientific) for 2 days to induce mesoderm specification ...
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bioRxiv - Molecular Biology 2022Quote: ... was co-transfected with either pre-gRNA or gRNA expression plasmids (1 µg per 6-well) using Lipofectamine 3000 kit (5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Molecular Biology 2022Quote: ... the cells were first transfected with the pcDNA3_CasRx-GFP plasmid (2.5 µg per 6-well) using Lipofectamine 3000 kit (5 µl Lipo 3000 and 5 µl P3000 reagents per 6-well; Thermo Fisher Scientific) according to the manufacturer’s guidelines ...
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bioRxiv - Biophysics 2020Quote: A 6-well plates (Nalge Nunc International, Roskilde, Denmark) was treated with Poly-L-Lysine (PLL ...
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bioRxiv - Developmental Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies #D1306) was used for nuclei staining in conjunction with secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... and 1.5 μg Random Primer Pd(N)6 (Invitrogen). RNA was denatured at 65°C for 2 min and then added to 40 U RNAse inhibitors (RNaseOUT-Invitrogen ...