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Citations for Thermo Fisher :
1 - 50 of 10000+ citations for 6 Bromo benzo c isothiazole since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... / bromo-chloro-indolyl phosphate (Thermo Scientific) in AP buffer or using Naphtol AS-MX phosphate and Fast Red (Sigma-Aldrich ...
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bioRxiv - Microbiology 2019Quote: ... from 25°C to 99°C in a Quant Studio 6 Flex System (Applied Biosystems) using the melting-curve setting ...
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bioRxiv - Neuroscience 2023Quote: ... at 37°C for 6 minutes at which point DMEM (Gibco) was added ...
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bioRxiv - Molecular Biology 2023Quote: ... for 6 h at 37 °C before lysis in DPBS buffer (Gibco), and then the expression of TGM2 was detected by western blot analyses with anti-TGM2 antibody (CST ...
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bioRxiv - Genomics 2023Quote: ... and extracted total RNA using BCP (1-bromo-3-chloropropane; Life Technologies, Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... and 0.175 g/ml 5-bromo-4-chloro-3-indolyl-phosphate (BCIP) (Invitrogen). Alkaline phosphatase staining reaction was proceeded o/n at RT ...
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bioRxiv - Plant Biology 2023Quote: ... for 40 cycles (15 seconds at 95°C, 1 minute at 60°C) in a QuantStudio 6 Flex instrument (ThermoFisher). Percentage of input was calculated as 100* e(CtIN – log2(DF ...
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Immunoresolvents Support Skeletal Myofiber Regeneration via Actions on Myeloid and Muscle Stem CellsbioRxiv - Immunology 2020Quote: Bone marrow was collected from tibias and femurs of 4-6 mo female C57BL/6 mice and cultured for 7 days at 37°C and 5% CO2 in DMEM (Gibco,11995-073) supplemented with 10% FBS ...
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bioRxiv - Molecular Biology 2023Quote: ... followed by 40 cycles of 95°C for 15 s and 60°C for 1 min (Applied Biosystems QuantStudio 6 Flex). For testing of U7 snRNA level in U7 KD cells ...
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bioRxiv - Immunology 2024Quote: ... 10 min at 95 °C followed by 40 cycles of 10 sec at 95 °C and 40 sec at 60 °C using the Quant 6 real-time PCR instrument (Applied Biosystems). Samples were considered positive for Giardia spp ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Immunology 2020Quote: ... Cells were maintained at 37°C and 6% CO2 FreeStyle 293 Expression Medium (Thermo Fisher) and 0.2% Penicillin/Streptomycin 7 days post transfection ...
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bioRxiv - Molecular Biology 2019Quote: Cells were pulse-labeled with the thymidine analog Bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrdU; ThermoFisher) for 2 hr (50mM ...
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bioRxiv - Cancer Biology 2023Quote: ... Recovered nuclei were stained with FITC-conujugated α-5-bromo-2’-deoxyurine (Invitrogen, MoBu-1) in staining buffer (2 mM HEPES pH 7.4 ...
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bioRxiv - Developmental Biology 2022Quote: ... 95 °C for 5 seconds and 60 °C for 20 seconds in a QuantStudio 6 Flex Real-Time PCR machine (Thermo Fisher Scientific, USA). The detectors used were FAM-BHQ1 and HEX- BHQ1 ...
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bioRxiv - Biophysics 2023Quote: ... Transfection lasted for 6 hours at 37°C and was terminated by replacing the medium with fresh 37°C Opti-MEM® (Life Technologies, ThermoFisher Scientific) supplemented with 50 μg/mL Gentamycin (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 30 sec and extension at 60 °C for 30 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
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bioRxiv - Immunology 2024Quote: ... followed by 40 cycles of denaturation at 95 °C for 5 sec and extension at 58 °C for 34 sec in the QuantStudio 6 system (Applied Biosystems Co., USA). At the end of PCR ...
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bioRxiv - Cancer Biology 2021Quote: ... HRas samples were heated at 95 °C for 6 min using a MiniAmp thermocycler (Applied Biosystems), resulting in protein denaturation ...
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bioRxiv - Genomics 2021Quote: ... enzymatic dissociation was performed for 4–6 minutes at 37°C in 1 mL TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 mL of TRIzol (Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... enzymatic dissociation was performed for 4–6 min at 37 °C in 1 ml TrypLE (Invitrogen), then cell pellets were washed with ice-cold PBS and lysed with 1 ml of TRIzol (Invitrogen) ...
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bioRxiv - Biophysics 2023Quote: ... Transfection lasted for 6 hours at 37°C and was terminated by replacing the medium with fresh 37°C Opti-MEM® (Life Technologies, ThermoFisher Scientific) supplemented with 50 μg/mL Gentamycin (Invitrogen) ...
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bioRxiv - Cancer Biology 2019Quote: Cells were labelled with 10 μM 5-bromo-2′-deoxyuridine (BrdU) (Thermo Fisher Scientific, Cat. #: B23151) for 30 min ...
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bioRxiv - Microbiology 2021Quote: ... 40 μg/mL 5-bromo-4-chloro-3-indolyl-beta-D-galactopyranoside (X-Gal) (Thermo Scientific) and 1 mM isopropyl beta-D-thiogalactopyranoside (IPTG ...
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bioRxiv - Genetics 2019Quote: ... except that 1-Bromo-3-chloropropane was used instead of chloroform and DNAse (TURBO DNase, Ambion) treatment was carried out ...
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bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
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bioRxiv - Cell Biology 2024Quote: ... Overexpressed SAS-6 was detected with mouse anti-c-Myc (1:250; 9E10; Invitrogen, UK; 13-2500) antibody ...
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bioRxiv - Microbiology 2021Quote: ... and 40 µg/mL X-Gal (5-bromo-4-chloro-3-indolyl-β-D-galactopyranoside; Thermo Scientific). Plates were incubated at room temperature for 48-72 h ...
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bioRxiv - Bioengineering 2020Quote: Before staining with either 5-bromo-4-chloro-3’-indolyphosphate and nitro-blue tetrazolium (BCIP/NBT, ThermoFisher) or Alizarin Red S (ARS ...
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bioRxiv - Microbiology 2024Quote: ... containing 100 mg/ml of X-gal (5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside) (Thermofisher) to confirm CFU/ml counts.
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bioRxiv - Biochemistry 2022Quote: ... and blotted for 4 to 6 seconds at 4°C and 100% humidity using the Vitrobot system (ThermoFisher), before plunging immediately into liquid ethane for vitrification ...
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bioRxiv - Neuroscience 2020Quote: ... treated coverslips and incubated at 37°C for 2-6 hours in Dulbecco’s modified Eagle’s medium (F-12/DMEM; Invitrogen) supplemented with 1% fetal bovine serum (FBS ...
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bioRxiv - Cell Biology 2020Quote: ... at 37°C and 5% CO2 with 6 mL HDF medium consisting of 1x Advanced DMEM (Gibco™), 1x Glutamax™ (Gibco™) ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no ...
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bioRxiv - Physiology 2024Quote: ... 6 rat samples (ExpA, B and C) were placed directly in Leibovitz’s L-15 medium (Gibco 11415-064) to develop organoids immediately ...
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bioRxiv - Biochemistry 2023Quote: ... Complexes were assembled for 30 min at 30 °C and separated on 6% polyacrylamide DNA retardation gels (Invitrogen). Gels were dried and analyzed by phosphorimaging (Fuji).
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bioRxiv - Immunology 2023Quote: ... 5-bromo-4-chloro-3-indolyl phosphate (BCIP)/nitro blue tetrazolium (NBT) substrate (Thermo Fisher Scientific, cat.: 34042) was added and the reaction was terminated after 5 min under running tap water ...
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bioRxiv - Plant Biology 2024Quote: ... or X-Gal (5-bromo-4-chloro-3-indolyl-b-D-galactopyranoside, W5376C; Thermo Fisher Scientific, Guilford, CT). GUS and/or LacZ-stained tissues were cleared for 30 s with 12 % sodium hypochlorite solution before microscopy observations.
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bioRxiv - Physiology 2020Quote: ... interleukin 6 (IL-6; Invitrogen, Carlsbad, CA), plasminogen activator inhibitor 1 (PAI-1 ...
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bioRxiv - Microbiology 2020Quote: ... Reactions were incubated at 37°C for 4 hours and run on a 6% TBE-Urea gel (Invitrogen, EC6265BOX). Bands of the appropriate size were excised ...
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bioRxiv - Microbiology 2019Quote: Bacterially expressed ZIKV EDIII proteins (C-terminal 6 × His-tag) were conjugated to Ni-NTA Magnetic beads (Thermo Scientific) following manufacturers protocol ...
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bioRxiv - Molecular Biology 2019Quote: ... SmBChE1 and SmAChE3 (fSmChEs) were EcoRI/XbaI cloned into the C-terminal 6-His-tagged pPICZαA expression vector (Invitrogen) to facilitate secretory expression ...
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bioRxiv - Microbiology 2021Quote: Bone marrow derived macrophages (BMDMs) were generated from 6-8 week old BALB/c mice in RPMI-1640 (Gibco) containing 10% foetal calf serum ...
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bioRxiv - Biochemistry 2022Quote: ... Samples were incubated at 37°C for 30 min and loaded onto 6% DNA retardation gels (Thermo Fisher Scientific). DNA was detected by staining the gel with GelRed (VWR) ...
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bioRxiv - Microbiology 2022Quote: ... 60 °C for 1 min) X 49 cycles on the QuantStudio 6 flex Real-time PCR system (Applied Biosystems), using the TaqMan RNA-to-CT 1-step kit (Applied Biosystems).
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... adult WT C57BL/6J mouse testes were dissected and incubated at 37 °C for 6 h in either Dulbecco’s Modified Eagle’s Medium/Nutrient Mixture F-12 (DMEM/F12, Thermo Scientific) or compound 5 (100 μM ...
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bioRxiv - Neuroscience 2023Quote: Human tissue blocks (N = 6) were stored at −80 °C and transferred to the cryostat (CryoStar NX70, Thermo Scientific) on dry ice ...
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bioRxiv - Molecular Biology 2023Quote: ... pH 6) at 40°C with vigorous shaking at 1400rpm for 30 minutes using a thermal mixer (Thermo Scientific). The eluted DNA and previous input samples were then amplified using the PCR Primer Mix 3 for Illumina and the VAHTS HiFi Amplification Mix 2 (ND607 ...
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bioRxiv - Neuroscience 2021Quote: ... a staining solution of 1 mg/mL 5-bromo-4-chloro-3-indolyl-beta-d-galactopyranoside (X-gal, Invitrogen), 1× citratesodium phosphate buffer (pH 6.0) ...
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bioRxiv - Pathology 2022Quote: ... as above and left to attach for 2 hours before adding bromodeoxyuridine / 5-bromo-2’-deoxyuridine (BrDU, Invitrogen B23151) to a final concentration of 75 µM and left to proliferate for 6 hours ...