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Citations for Thermo Fisher :
201 - 250 of 4324 citations for 6' HYDROXY DOXAZOSIN since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... and a QuantStudioI 6 Flex System (ThermoFisher). Specifics regarding cycle parameters and primers can be found in Supplementary Methods ...
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bioRxiv - Cell Biology 2022Quote: ... IL-6 (Invitrogen; cat. no 88-7064) and TNF-α (Invitrogen ...
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bioRxiv - Physiology 2022Quote: ... on a QuantStudio 6 system (Applied Biosystems) according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: ... 6 μl DNase I (Thermo Fisher Scientific) was added for additional 15 min ...
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bioRxiv - Molecular Biology 2023Quote: ... in a QuantStudio 6 system (Applied Biosystems). GAPDH was used as an internal control for normalization ...
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bioRxiv - Microbiology 2023Quote: ... using 6 μl of lipofectamine 2000 (Invitrogen). The lentiviral supernatant was harvested 48 h later and used for the transduction of THP-1 cells by spinoculation ...
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bioRxiv - Neuroscience 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) from ThermoFisher; anti-mouse IgG-horse radish peroxidase (HRP) ...
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bioRxiv - Molecular Biology 2022Quote: ... on a QuantStudio 6 instrument (Applied Biosystems). Gene expressions were normalized to endogenous GAPDH housekeeping gene ...
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Flaviviruses alter endoplasmic reticulum-mitochondria contacts to regulate respiration and apoptosisbioRxiv - Microbiology 2023Quote: ... 6’-diamidino-2-phenylindole (DAPI; Life Technologies) diluted 1/10,000 for nuclei staining ...
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bioRxiv - Cancer Biology 2023Quote: ... 6-diamidino-2-phenylindole (DAPI) (ThermoFisher, #D1306) at a concentration of 10 µg/ml in PBS / 3.0%BSA for 15 minutes then rinsed 3x 5 minutes in distilled water ...
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bioRxiv - Immunology 2023Quote: ... with a QuantStudio 6 Pro (Applied Biosystems). For murine Slc2a5 ...
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bioRxiv - Bioengineering 2023Quote: ... and a QuantStudio™ 6 instrument (ThermoFisher). Samples were activated at 94°C for 3 min ...
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bioRxiv - Developmental Biology 2023Quote: ... and 6 U/mL Dispase (Gibco, 17105041) for 1 hr at 37°C with constant agitation ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... using a QuantStudio 6 (Thermo Fisher Scientific). Relative LepR mRNA expression levels were determined using the 2−ΔΔCT method normalized to β-Actin mRNA expression levels ...
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bioRxiv - Cell Biology 2023Quote: ... puromycin (6 μg/ml; Thermo Fisher Scientific) was added to the select transduced cells ...
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bioRxiv - Genetics 2022Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). The Ct values were analyzed by the relative fraction of input method.
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bioRxiv - Genetics 2022Quote: ... or the QuantStudio 6 Flex (Thermo Fisher). Samples were analyzed using a two-step amplification and melt curves were obtained after 40 cycles ...
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bioRxiv - Developmental Biology 2023Quote: ... using QuantStudio 6 Flex system (Applied Biosystems). Gapdh was used as endogenous control and data analyzed with ΔΔCt method ...
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bioRxiv - Cell Biology 2023Quote: ... and a 6-well plate (ThermoFisher Scientific) were brought into a biosafety cabinet using aseptic techniques ...
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bioRxiv - Immunology 2023Quote: ... 6 U/ml DNase I (ThermoFisher Scientific) and 60 U/ml hyaluronidase (Millipore Sigma ...
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bioRxiv - Immunology 2023Quote: ... no UNG (Life Technologies, Quant Studio 6). Reactions were carried out for 40 cycles ...
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bioRxiv - Genomics 2023Quote: ... using a QuantStudio 6 FLEX machine (Thermofisher). See Supplementary Data for primers ...
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bioRxiv - Microbiology 2023Quote: ... 6’-diamidino-2-phenylindole (DAPI; Life Technologies) diluted 1:10000 in PBS ...
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bioRxiv - Neuroscience 2023Quote: ... in 6-well plates (Thermo Fisher Scientific). Each line was passaged with 0.5 mM EDTA (Life Technologies ...
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bioRxiv - Immunology 2024Quote: ... on the QuantStudio 6 platform (ThermoFisher Scientific). Ct values of Ifng were normalized to the Ct values for Gapdh ...
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bioRxiv - Microbiology 2023Quote: ... Novex TBE 6 or 10% gels (Invitrogen) were pre-run in 0.5x TBE running buffer (pH ∼8.4 +/- 0.5) ...
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bioRxiv - Physiology 2024Quote: ... on a QuantStudio 6 Pro (Applied Biosystems). Probes included ...
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bioRxiv - Immunology 2024Quote: ... 6 μl DNase I (Thermo Fisher Scientifiс) was added for additional 15 min ...
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bioRxiv - Immunology 2023Quote: ... 6 μl DNase I (Thermo Fisher Scientifiс) was added for additional 15 min ...
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bioRxiv - Cancer Biology 2019Quote: ... 300,000/6-well) and IDC (5,000/96-well; 100,000/6-well) cells were seeded in regular (Thermo Fisher Scientific) or ULA (Corning Life Sciences ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Cancer Biology 2020Quote: ... sgRNAs were forward transfected in 6-well dishes by using 6 μl of FuGENE® HD (PRE2311, Fisher Scientific) with 3 μg of sgRNA ...
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bioRxiv - Molecular Biology 2020Quote: ... for the first 24 hours and for further 4 to 6 days in Essential 6 media (Thermo Fisher Scientific).
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bioRxiv - Pathology 2020Quote: ... CHO cells were transfected with 5 ug of human cadherin-6 in pIRES-puro or mouse cadherin-6 in pCDNA3.1 via Lipofectamine 2000 (Invitrogen). After 48hrs ...
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bioRxiv - Molecular Biology 2021Quote: ... fluorescence-labeled HIV-1JR-FL Env(-) carrying the (His)6 epitope tag was incubated with biotin-conjugated anti-(His)6 tag antibody (HIS.H8, Invitrogen) at 4° for two hours.
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bioRxiv - Cell Biology 2020Quote: Cells in the 6-well plates were transfected with 2μg plasmid of AMPKα or OGG1 via 6 μl Lipofectamine 2000 (Invitrogen) for 6 hours following the manufacturer’s instructions ...
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bioRxiv - Genetics 2020Quote: ... The Cas9-sgRNA-containing plasmid and donor vector were co-transfected at a molar ratio of 1:6 into XX mESCs cells on a 6-well plate using Lipofectamine 2000 according to the manufacturer’s protocol (ThermoFisher). Transfected cells were passaged at different densities into three Petri dishes with feeders ...
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bioRxiv - Biochemistry 2021Quote: ... 6% native PAGE gels were prepared by first making a gel solution containing 6% acrylamide (29:1) (Fisher Scientific).15 This solution was then degassed under vacuum and mixing ...
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bioRxiv - Developmental Biology 2020Quote: Skin tissues collected from newborn Foxn1−/− (Cby.Cg-Foxn1
/cmdb) and Foxn1+/+ (C57BL/6; B6) mice were incubated in dispase (6 U/ml; Life Technologies) overnight at 4°C ... -
bioRxiv - Neuroscience 2022Quote: ... The final pellet was resuspended in 0.5 mL of NRB containing 6 μM 4’,6-diamidino-2-phenylindole (DAPI, ThermoFisher; D1306). The suspension was filtered through a 20 μm filter (Sysmex ...
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bioRxiv - Molecular Biology 2020Quote: ... 2:6 and 3:6 dilution ratios to allow efficient selection of Hygromycin B (Thermo Fisher Scientific Catalog Number: 10687010). The Hygromycin selection was started at the 48 hours after transfection time point with a final concentration of 150µg/ml and refreshed every 3-4 days until the control non-transfected cells on a separate plate were completely dead (takes approximately 3 weeks from the start of transfection until the cells are expanded and frozen) ...
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bioRxiv - Biochemistry 2021Quote: ... were incubated without ligand or with EGF (6 μM) or EREG (6 μM) in buffer A containing disuccinimidyl suberate (DSS) at 62.5 μM (ThermoFisher Scientific) for 30 min at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: Supernatant obtained from the BALF was used to measure the endogenous release of IL-6 using a commercially available Murine IL-6 ELISA kit according to the manufacturer’s instructions (ThermoFisher, InVItrogen). The absorbance was read at 450 nm and secreted IL-6 was analyzed using Gen5 data analysis software (BioTek Instruments ...
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bioRxiv - Microbiology 2022Quote: 293T-ACE2 and HT1080-ACE2 cells were seeded in 24-well plates and 6-well plates respectively to achieve 70% confluency after 4-6 hours and then transfected with Lipofectamine RNAiMAX (Thermofisher) using the indicated dsiRNAs (IDT ...
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bioRxiv - Cancer Biology 2020Quote: ... 293T cells were transiently transfected in 6-well plates with 6 individual IFNLR1-gRNA plasmids using Lipofectamine 3000 (Life Technologies). The cells were harvested 4 days post-transfection and subjected to fluorescence-activated cell sorting (FACS ...
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bioRxiv - Cancer Biology 2022Quote: ... and ran on QuantStudioTM 6 Flex Real-Time PCR System using QuantStudioTM 6 and 7 Flex Real-Time PCR software v1.0 (Applied Biosystems). Relative gene expression levels were quantified using β-actin or human TBP as housekeeping genes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Pro-inflammatory cytokine (IL-8) and (IL-6) release was determined using the IL-6 and IL-8 ELISA kit (Invitrogen) according to the manufacturer’s instructions (ThermoFisher).
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bioRxiv - Physiology 2020Quote: ... and incubated with 100 mM 6-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (6-NBDG) (Life Technologies) in 10 nM Tris/HEPES buffer containing 150 mM KCl or 150 mM NaCl for 30 minutes at 37 °C ...
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bioRxiv - Genetics 2021Quote: ... treated wild-type C57BL/6 and 12-week-old Rad1 CKO mice were subjected to phosphopeptide enrichment and 6-plex TMT (ThermoFisher) labeling (Sims et al. ...
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bioRxiv - Pathology 2022Quote: ... The concentrations of IL-6 and VEGFA were determined using an enzyme-linked immunosorbent assay (Mouse IL-6 ELISA kit, ThermoFisher; Mouse VEGF-A DuoSet ELISA kit ...