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Citations for Thermo Fisher :
401 - 450 of 10000+ citations for 58K Golgi protein Rabbit Recombinant mAb since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... or control rabbit IgG antibody and protein G beads (Invitrogen, 10003D) overnight at 4 °C while rotating ...
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bioRxiv - Cancer Biology 2023Quote: ... rabbit monoclonal anti-human p53 protein SP5 (1:200) (Invitrogen, USA) and mouse monoclonal anti-p63 antibody (1:500 ...
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bioRxiv - Bioengineering 2023Quote: ... and rabbit polyclonal cementum protein 1 (1:100, Thermo Fisher Scientific) prepared in a solution of 0.3% Triton X-100 ...
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bioRxiv - Bioengineering 2021Quote: ... recombinant human Vitronectin (Gibco A14700) was used at a final concentration of 25ug/ml ...
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bioRxiv - Cell Biology 2021Quote: ... Recombinant (Invitrogen, no. 10342-020). Glyceraldehyde-3-phosphate dehydrogenase (GAPDH ...
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bioRxiv - Cancer Biology 2020Quote: Human recombinant IL-1β (ThermoFisher) was reconstituted in deionised water and administered at 10 ng/ml.
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bioRxiv - Microbiology 2022Quote: Purified recombinant β-lactamase (ThermoFisher) in PBS (0.15 nM ...
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bioRxiv - Developmental Biology 2019Quote: ... and recombinant human FGF2 (Invitrogen) at 20 ng/ml ...
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bioRxiv - Physiology 2021Quote: ... Recombinant CSF1R (Invitrogen, Carlsbad, CA) was used at a concentration of 2.5 nM ...
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bioRxiv - Cancer Biology 2024Quote: ... 1% mouse recombinant EGF (Invitrogen, 5% Recombinant human R-spondin (Peprotech).
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bioRxiv - Cancer Biology 2024Quote: ... recombinant (Thermo Fisher Scientific, #10342020), and specific oligonucleotides (Supplementary Table S4 ...
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bioRxiv - Microbiology 2023Quote: ... containing PMA and ionomycin according to the manufacturer’s instructions and Golgi Plug (1 μl/sample) (Fisher Scientific; cat# BDB555029) for 4-6 hours at 37°C ...
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bioRxiv - Zoology 2022Quote: ... Cardiac muscle was detected with a rabbit recombinant polyclonal primary antibody to cardiac troponin I (cTnI, Invitrogen, 1HCLC, 1:600) and a donkey anti-rabbit antibody conjugated to the fluorophore Alexa 647 nm (Invitrogen A31573 ...
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bioRxiv - Microbiology 2020Quote: Recombinant expressions of proteins involved in this study were performed by using the ExpiCHO™ expression system (Thermo Scientific, A29133). Briefly ...
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bioRxiv - Genomics 2020Quote: ... Both cell lines were maintained in culture vessels coated with a matrix of Vitronectin human recombinant protein (ThermoFisher Scientific, A14700) and batch fed daily with mTeSR (STEMCELL Technologies ...
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bioRxiv - Microbiology 2021Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) or CaptureSelect™ IgA Affinity Matrix (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... Antibodies produced from cell culture supernatants were purified immediately by affinity chromatography using recombinant Protein G-Agarose (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... Recombinant E protein was produced by transient transfection of Expi293F cells using a ExpiFectamine 293 Transfection Kit (Thermo Fisher Scientific). Cell supernatants were harvested 4 days after transfection and then concentrated before exchange into 2× PBS at pH 6.5 and finally into 2× PBS at pH 8.0 ...
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bioRxiv - Cell Biology 2022Quote: Ribonucleoprotein complexes containing 3.9 picomoles of ATF3 sgRNA guides and 3 picomoles of purified recombinant Cas9 protein (Synthego) were transfected into HEK293T cells using Lipofectamine CRISPRmax (Invitrogen). 72 hours post transfection ...
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bioRxiv - Cell Biology 2022Quote: ... with Essential 8 Supplement (1X) in culture vessels coated with 5mg/ml vitronectin (Recombinant Human Protein; Life Technologies, London, UK). Spontaneous differentiation medium was Knockout DMEM ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were sonicated and recombinant proteins purified in a single step on Ni-agarose column according to manufacturer instructions (Invitrogen). Protein samples were dialyzed against PBS with 5 mM MgCl2 and 0.1% glycerol for 2h and used immediately in experiments ...
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bioRxiv - Microbiology 2021Quote: ELISA assays were performed in 96-microwell plates coated with 150 ng/well of SARS-CoV-2 Spike (aa 16-1213) His-tagged recombinant protein (ThermoFisher) and 1010 phage or AAVP particles/50 μL of PBS O.N ...
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bioRxiv - Microbiology 2019Quote: ... Stable cell lines expressing the recombinant protein were initially maintained in complete medium comprising of serum free medium Sf-900 II SFM (ThermoFisher) complemented with 10% v/v foetal bovine serum (Sigma) ...
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bioRxiv - Microbiology 2020Quote: ... recombinant spike protein and ACE2 was conjugated with EZ-Link™ Sulfo-NHS-Biotin (1:3 molar ratio; Thermo Fisher) in Dulbecco’s PBS at room temperature for 30 min ...
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bioRxiv - Microbiology 2020Quote: A baculovirus expression system was used for the expression of DUB2 recombinant protein in sf9 insect cells as described in the Bac-to-Bac Baculovirus Expression protocol (Invitrogen). The gene was first cloned into the PFastBacNKI-his-3C-LIC vector using Ligation Independent Cloning (LIC) ...
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bioRxiv - Microbiology 2019Quote: ... bacteria resuspended to OD660 0.3 were incubated with 1 µg of recombinant protein in the presence of 1x protease inhibitors (Pierce, Thermo Scientific) in sterile 1.5 ml microcentrifuge tubes with gentle end-over-end mixing for 30 min at 25°C.
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bioRxiv - Immunology 2021Quote: For competitive ELISA recombinant hACE2 protein were immobilized (400 ng per well) on immunoplates (Nunc MaxiSorp, Thermo Fisher Scientific, USA). RBD-specific nanobodies were mixed with HRP-conjugated recombinant RBD protein (0.2 μg/ml ...
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bioRxiv - Immunology 2021Quote: ... LPS-primed THP-1 cell lysates or purified recombinant NLRP3 protein were pre-cleaned using streptavidin magnetic beads (88816, ThermoFisher) to remove nonspecific binding ...
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bioRxiv - Immunology 2021Quote: ... The proteins were then purified by immobilized metal affinity chromatography by using HisPur Ni-NTA (Purify His-tagged recombinant fusion proteins using high-capacity nickel-nitrolotriacetic acid methal-chelate beads) spin columns (ThermoFisher). Recombinant proteins were refolded on the column by gradually removing urea until reaching to a concentration 0 M and then dialyzed against PBS ...
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bioRxiv - Cell Biology 2022Quote: ... They were adapted and then cultured in feeder-free conditions on culture dishes coated with the Vitronectin Recombinant Human Protein (ThermoFisher) in mTeSR1™ medium (StemCell Technologies ...
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bioRxiv - Cell Biology 2022Quote: ... Recombinant Breg and GFP protein was labeled with membrane non-permeable biotin according to the manufacturer’s instructions (Thermo Fisher Scientific).
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bioRxiv - Genomics 2022Quote: ... Recombinant protein was expressed in 8L culture of Spodoptera frugiperda SF9 cells using the Bac-to-Bac system (Thermo Fisher). Cells were cultured at 27°C ...
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bioRxiv - Plant Biology 2022Quote: ... NiHa-OSR and NoFa-ICL: Fractions containing recombinant protein were pooled and dialyzed using a Slide-A-Lyzer (ThermoFisher Scientific) cassette (20 kDa MWCO ...
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bioRxiv - Cancer Biology 2022Quote: Recombinant human SHBG (ProSpec, Israel) was directly labeled with Alexa Fluor-555 using a protein conjugation kit (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2023Quote: ... The recombinant GFP and RPH1 proteins were enriched and purified by affinity chromatography using Ni-NTA Superflow resin (Thermo Scientific). Protein concentrations were calculated based on a standard curve created using bovine serum albumin (BSA ...
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bioRxiv - Genetics 2023Quote: ... All iPSC lines were maintained on Nunc 6-well culture plates (ThermoScientific #140675) coated with recombinant human vitronectin protein at a final concentration of 0.50 ug/cm2 (Gibco #A14700) in complete Essential 8 Medium (complete E8 ...
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bioRxiv - Plant Biology 2023Quote: ... Recombinant GST-tagged proteins purification were performed according to the manufacturer’s instructions of PierceTM Glutathione Superflow Agarose (Thermo Fisher Scientific), and recombinant MBP-tagged proteins purification were performed according to the manufacturer’s instructions of Amylose Resin (NEB).
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Bioengineering 2023Quote: ... They were cultured on 100-mm Petri dishes coated with human VTN-N truncated recombinant protein (Gibco, Thermo Fisher Scientific). The hiPSCs obtained from Thermo Fisher Scientific were maintained in Essential 8 Medium (Gibco ...
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bioRxiv - Molecular Biology 2023Quote: Recombinant Rpb4/Rpb7-6His protein (5 μg) was incubated in a 50 μL slurry of HisPur Cobalt Resin (Thermo Scientific) in binding buffer (20 mM Tris [pH 7.5] and 50 mM NaCl ...
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bioRxiv - Biochemistry 2023Quote: Plasmids for recombinant protein expression were constructed using mega primer insertion PCR [64] using the plasmid Champion pET303/CT-His vector (Invitrogen). The double-stranded DNA inserts (Integrated DNA Technologies ...
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bioRxiv - Immunology 2022Quote: ... and anti- TCRα (clone H28-710 or H28) mAbs were obtained by digesting commercial full-length mAbs using Fab preparation kit (Thermo Fisher 44685). H57 scFv was a generous gift from Johannes Huppa of the Medical University of Vienna (10).
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bioRxiv - Neuroscience 2021Quote: ... anti-Aβ42 mAb (H31L21, Invitrogen, cat 700254, 1:200); anti-Aβ40 pAb (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... HRP-conjugated anti-mouse IgG mAb (Invitrogen, Carlsbad, CA); HRP-conjugated anti-mouse IgG antibody (Dako ...
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bioRxiv - Microbiology 2021Quote: ... slides were probed with anti-LPS Mab (ThermoFisher Scientific) at 1:200 dilution ...
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Structure and Neutralization Mechanism of a Human Antibody Targeting a Complex Epitope on Zika VirusbioRxiv - Microbiology 2022Quote: ... mAbs were serially diluted in DMEM (Life Technologies, 11330032) media supplemented with 2% Fetal Bovine Serum (Sigma ...
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bioRxiv - Cancer Biology 2019Quote: ... EGFR mouse mAb (Thermo Fisher, 199.12, Cat# MA5-13319), 1:100 ...
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bioRxiv - Immunology 2020Quote: ... The mAb CL2/6 (Thermo Fisher Scientific, Waltham, MA) recognizes human E-selectin and canine L-selectin [19] ...
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bioRxiv - Immunology 2020Quote: ... Pierce Rapid ELISA Mouse mAb Isotyping kit (ThermoFisher, USA) was used ...
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bioRxiv - Synthetic Biology 2022Quote: ... or the mouse mAb FB11 (ThermoFisher; cat # MA1-7388) that specifically recognizes F ...