Labshake search
Citations for Thermo Fisher :
501 - 550 of 10000+ citations for 5 tert butoxy 5 oxopentanoic acid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Immunology 2023Quote: ... and STX-S_pLenti at a ratio of 5:5:1 using Lipofectamine 3000 (ThermoFisher, L3000008) according to the manufacturer’s instruction ...
-
bioRxiv - Biochemistry 2023Quote: ... and a C18 PepMap 100 trap column (5 mm × 300, 5 μm, Thermo Fisher Scientific). Samples were eluted over a 90 min gradient from 0 to 35% acetonitrile at a flow rate of 0.3 μL/min ...
-
bioRxiv - Cell Biology 2023Quote: ... Peptides were enriched with μ-Precolumn (0.3 mm i.d. × 5 mm, 5 μm, Thermo Scientific) and separated on an AURORA column (0.075 mm i.d ...
-
bioRxiv - Neuroscience 2023Quote: ... Samples (5 μl aliquot) were normalized to 2-5 nM with Nuclease-free Water (Ambion), then 2 μl from each sample within one 96-index set was pooled to a total of 192 μl at 2-5 nM concentration ...
-
bioRxiv - Biochemistry 2023Quote: hSSB2 was labeled on intrinsic cysteines using 5-IAF dye (5-iodoacetamido-fluorescein, Thermo Fisher). Before labeling ...
-
bioRxiv - Cancer Biology 2021Quote: ... 4mM L-Glutamine and 1X ITS (5 μg/mL insulin, 5 μg/mL transferrin, 5 ng/mL selenium) (all from Gibco, Thermo Fisher Scientific, Waltham, MA). The Wit49 cell line was a gift from Dr ...
-
bioRxiv - Molecular Biology 2019Quote: ... TERT-RPE-1 cells were cultured in DMEM-F12 (Gibco 10565-018) supplemented with 10% FBS (Gibco 10270106).
-
bioRxiv - Cell Biology 2019Quote: ... Human TERT-RPE cells were cultured in DMEM+F12 (1:1) (Invitrogen) containing 10% FBS ...
-
bioRxiv - Neuroscience 2023Quote: ... and Tert was used as the reference gene (VIC labeled: ThermoFisher, 4403316).
-
bioRxiv - Cell Biology 2024Quote: ... HEKn and N/TERT keratinocytes were routinely cultured in EpiLife medium (ThermoFisher Scientific Cat#MEPI500CA ...
-
bioRxiv - Plant Biology 2019Quote: To assess GUS expression driven by the maize ubiquitin promoter in transgenic barley transformed with the pBRACT214m-GUS construct we collected different tissues and stained these with 1mg/ml of X-gluc (5-bromo-4-chloro-3-indolyl-B-D-glucuronic acid, Thermo Scientific, USA) in X-gluc buffer (100mM sodium phosphate buffer pH 7.0 ...
-
bioRxiv - Physiology 2019Quote: ... to obtain images and then stored at 4°C in 5% acetic acid and 10% glycerol solution in a heat-sealable roll stock pouches (Fisher Scientific, #0181226E). Proteins in gels used for Western Blotting were then transferred to polyvinylidene difluoride membranes (Whatman) ...
-
bioRxiv - Neuroscience 2019Quote: ... essential medium non-essential amino acids solution (1/100) and 5% (v/v) heat inactivated fetal bovine serum (Invitrogen, Paisley, Scotland, UK) and were maintained at 37°C in an atmosphere with 5% CO2 ...
-
bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
-
bioRxiv - Cell Biology 2020Quote: ... Samples were resuspended in 35μl of 1% formic acid and 15 μl were loaded onto a trap column (PepMap nanoViper C18 column, 5 μm, 100 Å, Thermo Scientific) equilibrated in 0.1% TFA ...
-
bioRxiv - Microbiology 2020Quote: Treated and untreated biofilm-containing pegs after a 24-hour incubation in MMBC-3 were subjected to microscopic imaging after staining with 5 μM of Syto®9 green-fluorescent nucleic acid stain (Invitrogen, ThermoFisher Scientific) diluted in PBS ...
-
bioRxiv - Cancer Biology 2021Quote: ... 50 nM of scrambled control or 5’-tRFCys antisense locked nucleic acid (LNA) oligos were transfected into cells with Lipofectamine 2000 (Thermo Fisher Scientific). Experiments were performed 24-48 hours post transfection ...
-
bioRxiv - Immunology 2021Quote: ... peptides (1 μg) dissolved in 5% formic acid and analyzed by LC-MS using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific) equipped with a Dionex ultra-high-pressure liquid chromatography system (RSLCnano).
-
bioRxiv - Physiology 2022Quote: ... were homogenized by ultrasonication (Q55 Sonicator, Qsonica Inc., USA) in 5 volumes of 1 M perchloric acid (PCA; A228 Fisher Scientific, USA). The samples were neutralized by adding KHCO3 ...
-
bioRxiv - Developmental Biology 2023Quote: ... Embryos were washed three times for 5 min with TBST and nuclei were stained with DAPI nuclei acid stain (ThermoFisher Scientific, D1306) for 30 minutes before a final round of three times 5 minute washes in TBS-T.
-
bioRxiv - Cancer Biology 2023Quote: Dried pellets were resuspended in LC/MS grade water:methanol (95:5) (Optima, Fisher Chemical) with 0.1% formic acid (Thermo Fisher Scientific Cat. 28905). Untargeted metabolomics analyses were performed using a Q-TOF (Agilent ...
-
bioRxiv - Molecular Biology 2023Quote: ... The 24 fractions were subsequently dried and the peptides re-dissolved in 5% formic acid and analyzed by nanoLC–MS/MS system Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific), equipped with an UltiMate 3000 RSLCnano Liquid Chromatography System ...
-
bioRxiv - Biophysics 2024Quote: Quantitative determination of intracellular pH (pHi) was performed using the cell-permeant ratiometric pH indicator SNARF™-5F 5-(and-6)-carboxylic acid AM (Thermo Fisher) in live imaged N2a cells at 48 hours of differentiation ...
-
bioRxiv - Cell Biology 2024Quote: ... 12 alternating fractions were resuspended in 2% formic acid/5% acetonitrile and subsequently injected for analysis on an Orbitrap Eclipse Tribrid (Thermo Fisher Scientific). For the yeast cell PISA ...
-
bioRxiv - Cell Biology 2023Quote: ... Samples were resuspended in Mass Spectrometry buffer (5% Formic Acid and 5% Acetonitrile) and were analyzed on a Liquid Chromatography Orbitrap Fusion Lumos Tribrid Mass Spectrometer (#IQLAAEGAAPFADBMBHQ, Thermo Fisher Scientific) equipped with a nano-Acquity UPLC system and an in-house developed nano spray ionization source ...
-
Turanose induced WOX5 restores symbiosis in the Medicago truncatula cytokinin perception mutant cre1bioRxiv - Plant Biology 2020Quote: ... and (MtWOX5) 5’-CACCATGCAGACGGTCCGAGATCTGTC-3’ and 5’- CCTTCGCTTAAGTTTCATGTAA-3’ (AhWOX5) and cloned into pENTR-dTOPO (Invitrogen). The entry clones of AhWOX5 & MtWOX5 recombined through LR clonase Gateway technology (Invitrogen ...
-
bioRxiv - Molecular Biology 2021Quote: ... Cells were selected 5 days after the transfection with 5 µg/mL blasticidin (Thermo Fisher Scientific). They were then seeded at low density and single cell colonies were picked and genotyped by PCR ...
-
bioRxiv - Physiology 2020Quote: ... Eyes were decontaminated by submersion in 5% povidone-iodine ophthalmic solution (Betadine 5%, Fisher Scientific, NC9771653) for two minutes ...
-
bioRxiv - Molecular Biology 2019Quote: ... the PCR products obtained with 2 primers (AV48_SMCHD1_gPCR_F: 5’-AGGAGCGCGTTTGAATCGG-3’, AV47_SMCHD1_gPCR_R 5’-CTTCGCGTACCTGACACACAC-3’) were TOPO-cloned (Thermo Fisher, #450071) and sent for sequencing.
-
bioRxiv - Genomics 2019Quote: ... after which cells were frozen at 5 × 106 cells/mL in 5% DMSO (Thermo Fisher Scientific). For the MPRA ...
-
bioRxiv - Microbiology 2020Quote: ... 5% CO2 and 5% O2 at 37°C in RPMI 1640 containing AlbuMAXII (Thermo Fisher Scientific) supplemented with 2 mM L-glutamine ...
-
bioRxiv - Molecular Biology 2022Quote: ... siM1-4: 5’ -ACTGGTAGCTTATTAAAGATT- 3’) and the HOXA1 siRNA (5’ - AGAACTTCAGTGCGCCTTATT- 3’) were purchased from Invitrogen™ (Silencer® Select siRNA ...
-
bioRxiv - Biochemistry 2022Quote: ... C18 trap (300 μm i.d x 5 mm, C18 PepMap100, 5 μm, 100 Å; Thermo Scientific) analytical columns (Acclaim PepMap TM RSLC ...
-
bioRxiv - Cell Biology 2023Quote: ... Target mtDNA gene (Forward primer: 5′-CACCCAAGAACAGGGTTTGT-3′, Reverse primer: 5′-TGGCCATGGGTATGTTGTTAA-3′, Invitrogen custom primers) and reference 18S ribosomal RNA gene (Forward primer ...
-
bioRxiv - Immunology 2023Quote: ... 5-fold serial dilutions of serum (assay diluent: PBS + 5% goat serum [Gibco] + 0.05% Tween-20) was added and incubated for 2 hours at 37°C (IgG ...
-
bioRxiv - Genetics 2023Quote: ... non-specific sites were blocked with PBS/5% FBS/5% NGS (normal goat serum, Life technologies) for 1 h at RT ...
-
bioRxiv - Biochemistry 2024Quote: ... blotted with blot force of 5 for 5 s using a Vitrobot Mark IV (Thermo Fisher) at 95% humidity and 4 °C ...
-
bioRxiv - Cancer Biology 2024Quote: ... Spheroids were split every 5 days using 5-min incubation with TrypLE™ Express (Thermo Fisher) at 37 ℃ ...
-
bioRxiv - Microbiology 2024Quote: ... This was followed by adding TMB (3, 3, 5, 5′-tetramethylbenzidine) peroxidase substrate (Thermo Fisher Scientific) for about 10 min ...
-
bioRxiv - Cancer Biology 2024Quote: ... 5 x 105 KMS-11 cells were labelled with 5 µM CellTrace™ Blue dye (Invitrogen) for 20 min at 37°C in the dark ...
-
bioRxiv - Plant Biology 2020Quote: ... adenosine-5′-monophosphate disodium (Fisher Scientific), cytidine-5′-monophosphate disodium (Chem-Impex) ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 ml Fetal Bovine Serum (Gibco), 250 μl of 1 μg/ml insulin (Sigma-Aldrich I6634 ...
-
bioRxiv - Cancer Biology 2021Quote: ... 5 mM glutamine (Gibco, Life Technologies) and 10% FCS (Pan Biotech).
-
bioRxiv - Cancer Biology 2021Quote: ... 5 mM glutamine (Gibco, Life Technologies) and 10% FCS (Pan Biotech).
-
bioRxiv - Cell Biology 2020Quote: ... with 5% fetal bovine serum (GIBCO), supplemented with endothelial growth medium (EGM)-2 SingleQuots (LONZA Clonetics CC-4176 ...
-
bioRxiv - Microbiology 2020Quote: ... 5 U RNase A (Thermo Scientific) and 2.5 U RNase H (Thermo Scientific ...
-
bioRxiv - Cell Biology 2020Quote: ... supplemented with 5% horse serum (Invitrogen), 20ng/mL epidermal growth factor (EGF) ...
-
bioRxiv - Cell Biology 2020Quote: ... 5% Horse Serum (Invitrogen #16050-122), 20 ng ml-1 EGF (Invitrogen #PHG0311) ...
-
bioRxiv - Developmental Biology 2021Quote: ... and 5% foetal bovine serum (Gibco) in an incubator filled with 45% N2 ...
-
bioRxiv - Molecular Biology 2021Quote: ... 5 µl Protein G Dynabeads (Invitrogen). Aforementioned volumes of magnetic beads were washed twice with 200 µl ChIP buffer 1 with BSA ...