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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for lefty A TGF beta 4 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: ... in HepG2 medium (Advanced MEM minus L-Glutamine [Gibco] + 10% [v/v] HI-FBS [Gibco] + 1% [v/v] penicillin-streptomycin + 2 mM Glutamax [Gibco] and 0.1% [v/v] amphotericin B). Once the HepG2 or HC-04 cells were grown to the desired concentration ...
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bioRxiv - Molecular Biology 2021Quote: ... blotted for 4 seconds at 4 °C in 100% humidity and plunge-frozen in liquid ethane by using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Bioengineering 2019Quote: ... Collected samples were analyzed using native gel electrophoresis (NativePAGE 4-16% Bis-Tris Gel) or denature gel electrophoresis (SDS-PAGE, Novex 4-20% Tris-Glycine Mini Gels, Thermo Fisher Scientific). For SDS-PAGE ...
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bioRxiv - Biochemistry 2020Quote: ... of HLC protein extract in 4 × Sample Loading Buffer (Li-Cor, 928-40004) were loaded onto NuPAGE™ 4-12% Bis-Tris Protein Gels (Invitrogen, NP0326BOX). Following resolution ...
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bioRxiv - Molecular Biology 2021Quote: ... antibodies overnight at 4°C and then incubated for an additional 3 hours at 4°C with 50 μL DynabeadsTM protein G (Life Technologies, 10004D). Following three washes with 1% Triton-TBS lysis buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Samples equivalent to 100 μg of DNA were incubated overnight at 4°C with 4 μg of rabbit polyclonal anti-ATF7IP (Invitrogen, PA5-54811), rabbit monoclonal anti-JUNB (C37F9 ...
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bioRxiv - Biophysics 2020Quote: ... Grids were blotted for 2-4 seconds at a blotting force of 4 and plunge-frozen in liquid ethane using a MarkIV Vitrobot (Thermo Fisher Scientific). The chamber was maintained at 8 °C and 100% humidity during freezing ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... We plated 4*105 cells/well of isolated cardiomyocytes to fibronectin (cosmo bio SFN01)-coated 4 well chamber slides (Thermo Fisher 154526) and cultured them in a 5% CO2 incubator ...
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bioRxiv - Developmental Biology 2022Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes (min ...
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bioRxiv - Microbiology 2020Quote: ... Fungal hyphae were stained with 0.5 mM 4.4-difluoro-5-methyl-4-bora-3a,4a-diaza-s-indacene-3-dodecanoic acid (C1-BODIPY 500/510 C12, Thermo Fisher Scientific) or 4.4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16 ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Molecular Biology 2020Quote: ... Lysates were centrifuged at 17,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific) containing 1% (v/v ...
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bioRxiv - Immunology 2020Quote: ... 2-4 × 105 cell equivalents per well were loaded into a NuPAGE 4-12% Bis-Tris density gradient gel (Thermo Fisher Scientific) and ran at a constant 150V for 80 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... cells were loaded with the calcium indicator Fluo-4 by incubating in supplemented NB medium that contained 2 µM of Fluo-4 AM (Invitrogen, Carlsbad, CA), and Pluronic F-127 (0.04% ...
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bioRxiv - Cell Biology 2020Quote: Cells were plated at a density of 4 × 104 cells per well in a 4 well chamber slide (Nunc Lab-Tek II Chamber slide system, Thermo Fisher Scientific), and cultured in the 20 mM sodium acetate-supplemented culture medium (10% FBS) ...
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bioRxiv - Biochemistry 2021Quote: Images displayed in Figure 4 and negative stain images in supplementary Figure 4 were acquired using a Tecnai TF20 electron microscope (Thermo Fisher, USA) equipped with FEG source operating at 200 keV ...
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bioRxiv - Molecular Biology 2021Quote: ... Purified eIF1A derivatives were labeled at 4 °C with 4-fold excess of Alexa Fluor™ 555 C2 maleimide (Alexa555, Thermo Scientific) following the manufacturer’s manual ...
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bioRxiv - Immunology 2022Quote: ... or corresponding isotype control antibodies diluted in FACS buffer for 30 min at 4 ° After fixation and permeabilization for 30 min at 4 °C using the Foxp3/Transcription Factor Staining Buffer Set (Thermo Fisher Scientific), resuspended cells were kept overnight at 4 °C ...
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bioRxiv - Cell Biology 2023Quote: ... the grid was blotted for 4 sec under 100% humidity at 4°C and then plunged into liquid ethane using Vitrobot Mark IV (Thermo Fisher Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... Human naive PSCs were passaged as single cells every 4 days at split ratio 1:3 or 1:4 following dissociation with TrypLE (Gibco 12563-029) for 10 minutes at room temperature (RT) ...
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bioRxiv - Immunology 2023Quote: ... the C-terminal FLAG-tagged WT sCTLA-4 and Y139A sCTLA-4 in pCMV-Tag4A were sub- cloned into the pFastBac vector (Thermo Fisher Scientific) to generate pFastBac-WT_sCTLA- 4-FLAG and pFastBac-Y139A_sCTLA-4-FLAG.
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bioRxiv - Neuroscience 2023Quote: ... The neuronal cultures were washed twice for 10 minutes with HBSS and then loaded with 4 μM of Fluo-4 AM (Molecular probes, F14201) in HBSS having 0.002% Pluronic F-127 for 30 min at room temperature ...
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bioRxiv - Bioengineering 2023Quote: ... Then the cells were stained with a solution of 4 mM calcein AM (TRC, #C125400) and 4 mM ethidium homodimer-1 (Thermo Fisher, #L3224) dissolved in PBS to identify live and dead cells ...
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bioRxiv - Molecular Biology 2023Quote: ... 3D artificial muscles were fixed with buffered 1% paraformaldehyde (PFA) (ThermoScientific, JI9943.K2) overnight at 4°C followed by 6 hours of blocking at 4°C [10% FBS (Gibco, 10270-106), 1% BSA (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... The pellets were washed twice and cell surface AF488 fluorescence was quenched by incubation for 30 min at 4 °C with rabbit anti-AF488 IgG (4 µg/mL, Thermo Fisher Scientific). The difference between the signals from the cells incubated in the absence and presence of anti-AF488 IgG corresponded to apoA-I bound to the cell surface.
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bioRxiv - Molecular Biology 2023Quote: ... Lysates were centrifuged at 20,800 × g for 5 min at 4°C and supernatants were mixed with NuPAGE LDS Sample Buffer (4×) buffer (Thermo Fisher Scientific). For SDS-PAGE ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... and the airway and vessel-free parenchyma was further minced 4 x 4 mm pieces and cryopreserved in high glucose DMEM GlutaMAXTM (Thermo Fisher Scientific) containing 10% DMSO (Carl Roth ...
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bioRxiv - Cell Biology 2024Quote: ... 10 μl of this mixture was loaded into the lanes of a precast 4-12% gradient polyacrylamide gel (4–12% Bolt Bis-Tris Plus Gels, Thermo Fisher Scientific).
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bioRxiv - Genetics 2024Quote: Cells were grown on 1.5mm coverslips coated with matrigel (iMGLs). Cells were first fixed in a 4% formaldehyde (Electron Microscopy cat. # 15714) solution in 4% sucrose in PBS (Thermo Fisher Scientific) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2020Quote: ... 4 μl of 5 mg/ml linear acrylamide (Ambion), 600 μl of preheated (65°C ...
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bioRxiv - Molecular Biology 2020Quote: ... 1.2 μl 1:4 (Sequenase:Sequenase dilution buffer (Thermo Fisher)) diluted Sequenase was added to reactions and incubated at 37°C for 8 minutes and 94°C for 8 minutes prior to transfer to ice.
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bioRxiv - Cancer Biology 2021Quote: ... Samples were loaded on Bolt 4-12% gels (Invitrogen) and transferred onto PVDF membrane (Invitrogen) ...
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bioRxiv - Developmental Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies #D1306) was used for nuclei staining in conjunction with secondary antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... 4 μl 5x First-strand buffer (Thermo Fisher Scientific), 2 μl 0.1 M DTT (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... Fluo-4 and Fura Red calcium indicators (ThermoFisher Scientific) were resuspended in DMSO to 1 mM prior to dilution and cell staining ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were fixed by 4% PFA in DPBS (Gibco) for 20 min and washed by DPBS three times.
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bioRxiv - Cell Biology 2020Quote: ... Frozen 4% PFA fixed OCT (Fisher Scientific, Hampton, NH) sections were placed on slides ...
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bioRxiv - Immunology 2021Quote: A 4-Plex ProcartaPlex kit (Thermo Fisher Scientific, Inc.) was used to measure IL-6 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and a Qubit™ 4 fluorometer (Invitrogen, Carlsbad, CA). While a given tissue from a specimen pool representing a given locality was sequenced only once in this format ...
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bioRxiv - Developmental Biology 2020Quote: ... lipophilic styryl dye FM 4-64FX (Thermo Fisher Scientific) was injected in the body cavity of females in a 1:500 dilution (3 μg/μl ...
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bioRxiv - Developmental Biology 2020Quote: ... 50 μg Fluo-4 AM cell-permeant dye (Invitrogen) was diluted in 10 μl of 20% Pluronic F-127 in DMSO ...
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bioRxiv - Developmental Biology 2021Quote: ... SCOs were fixed in 4 % paraformaldehyde (Thermo Fisher Scientific) in 1X PBS for 10 min and mounted directly in Fluoromount G Mounting Medium (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2022Quote: ... and Falcon 4 Direct Electron Detector (Thermo Fisher Scientific) operated in Electron-Event representation (EER ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 nmol of each dNTP (Thermo Fisher Scientific, Lithuania) and water to final volume of 20μl ...
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bioRxiv - Neuroscience 2020Quote: ... or NuPAGE 4-12% Bis-Tris gel (Invitrogen #NP0322), transferred to a nitrocellulose membrane (250mA ...
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bioRxiv - Neuroscience 2021Quote: ... 2 M thiourea and 4% CHAPS (Thermo Fisher Scientific). Each combined replicate of samples was loaded onto a QIAshredder spin column (QIAGEN ...
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bioRxiv - Bioengineering 2022Quote: ... and OptiPRO SFM medium (4% final concentration, Thermo Fisher) were sequentially added ...
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bioRxiv - Cell Biology 2022Quote: Precast polyacrylamide gels (Bolt 4–12% Bis-Tris, Invitrogen) were used for all SDS-PAGE experiments ...
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bioRxiv - Cell Biology 2022Quote: ... 4 mM of L-Glutamine (Gibco, St. Louis, USA), 200 U/ml of penicillin and 200 μg/ml of streptomycin (Gibco ...
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bioRxiv - Developmental Biology 2022Quote: ... diluted in 1 x PBS (Fisher Scientific, BP399-4). After 24 h of fixation ...