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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for Zika Virus NS1 Protein Uganda strain since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... bovis BCG (Pasteur strain) was grown in Middlebrook 7H9 broth supplemented with Middlebrook oleic acid-albumin-dextrose-catalase (OADC) enrichment (Invitrogen Life Technologies, Carlsbad, CA), 0.002% glycerol ...
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bioRxiv - Molecular Biology 2022Quote: Single colonies of the desired strain were used to inoculate 10 mL of Buffered Glycerol Complex Medium (BMGY) (Pichia Expression Kit, Life Technologies, Carlsbad, CA, USA) and grown at 28°C for two days with shaking at 250 rpm ...
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bioRxiv - Cell Biology 2023Quote: ... ATPTG8Ty/cKD or ATPTG9Ty/cKD strains were treated with ATc or vehicle control (ethanol) for 72 hours then stained with 50nM MitoTracker DeepRed (Life Technologies, cat. no. M22426) for 1 hour at 37ºC and 5% CO2 ...
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bioRxiv - Microbiology 2023Quote: ... and the optical density was measured at 600nm to access the growth of both strains using spectrophotometer (Thermo Fisher Scientific GENESYS 10S UV-Vis). The loosely adhered cells were removed by washing the tubes carefully with MQ three times ...
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bioRxiv - Cell Biology 2023Quote: ... the HF and LF regions for cell isolation were identified by perfusion of whole globes with CellMask™ Orange plasma membrane strain (ThermoFisher, Waltham, MA, USA) for 90 min prior to dissection as described previously (Staverosky et al. ...
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bioRxiv - Microbiology 2024Quote: ... and their respective complemented strains cultured in 7H9 medium supplemented with 0.4% glycerol or sodium propionate were treated with CellROX Green (Life Technologies; final concentration, 5 μM) for 30 min at 37 °C ...
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bioRxiv - Plant Biology 2020Quote: ... the protein concentration was determined using the bicinchoninic acid assay (BCA Protein Assay Kit by Thermo Scientific Pierce). Volumes corresponding to 30 µg of protein were separated by SDS-PAGE ...
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bioRxiv - Biophysics 2021Quote: ... 2005) at 280nm and TCF7L2 proteins concentration was measured using the Pierce™ BCA protein Assay Kit (ThermoFisher) ion-exchange-ninhydrin analysis performed by Dr ...
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bioRxiv - Cancer Biology 2021Quote: ... protein concentrations were determined in situ for each well using a standard BCA protein assay (Thermo Scientific Pierce). The OCR and PPR values were normalized to µg protein ...
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bioRxiv - Molecular Biology 2020Quote: ... Protein concentrations were quantified using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Waltham, MA, USA).
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bioRxiv - Cancer Biology 2021Quote: ... Protein extracts were clarified and concentrations were measured with Pierce Coomassie Plus Protein Assay reagent (Thermo Fisher Scientific). Lysates were then resolved on SDS-PAGE gels (BioRad) ...
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bioRxiv - Cell Biology 2020Quote: ... protein lysates were immunoprecipitated with anti-GFP antibody conjugated to protein A-coupled polyacrylamide beads (#53142 Thermo Scientific) for 2 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... Protein quantification of lysates was done with the Micro BCA™ Protein Assay Kit (Thermo Fisher Scientific, #23235) and 10 µg of total protein was loaded into NuPAGE™ 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: The protein concentration of lysates was measured using the Pierce Coomassie Bradford protein assay kit (Thermo Fisher Scientific). Final protein concentrations were adjusted to 13 μg/μl in lysis buffer and NuPAGE 4x LDS sample buffer (NP0007 ...
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bioRxiv - Cell Biology 2019Quote: ... Protein concentrations of adult fly mitochondrial preps were determined with a BCA Protein Assay Kit (Thermo Scientific, USA) following manufacturer instructions ...
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bioRxiv - Cell Biology 2020Quote: ... Sample protein concentrations were determined with a Pierce BCA protein assay kit (Thermo Fisher Scientific, Waltham, MA, USA) and the samples were normalized and diluted to 5 µg protein/10 µl with 3x Laemmli sample buffer (30 % glycerol ...
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bioRxiv - Cell Biology 2020Quote: Estimations of protein concentrations were made using a Pierce BCA Protein Assay Kit (Thermo Fisher Scientific Cat# 23225). Cells were lysed for Western blot analysis in 2.5% (w/v ...
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bioRxiv - Developmental Biology 2020Quote: Protein concentration for all samples was quantified by Pierce™ BCA Protein Assay Kit (Thermo Fisher, Waltham, MA). All samples were processed for analysis on SDS-PAGE ...
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bioRxiv - Genomics 2020Quote: ... The concentration of each protein extract was determined using the Pierce BCA Protein Assay Kit (Thermo Fisher Scientific). For fractionation ...
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bioRxiv - Neuroscience 2022Quote: ... The supernatant was forwarded to protein concentration assessment using Pierce™ 660nm Protein Assay Reagent (Thermo Scientific™). Lysates of ATG5 KO mice were mixed with brain lysates of isotope labelled mice in a ratio of 1:1 (1 mg:1 mg) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were quantified by total protein content using a BCA protein assay (cat. no. 23228, Thermo Fisher Scientific) and diluted with 1X PBS to achieve a target dose concentration of 2 μg/ul ...
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bioRxiv - Molecular Biology 2021Quote: ... Samples were briefly sonicated and protein extracts quantified using the Pierce BCA Protein assay kit (Thermo Fisher Scientific). After equalizing the amounts of protein ...
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bioRxiv - Molecular Biology 2021Quote: ... Protein concentrations were determined using the Pierce™ BCA Protein Assay Kit (Thermo Fisher Scientific, Bleiswijk, the Netherlands). Protein samples were separated on a NuPAGE™ 4–12% Bis-Tris Protein Gel (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... membrane fractions were normalized for protein content using the Pierce BCA Protein Assay Kit (Cat#23227, ThermoFisher Scientific), subjected to trypsinization ...
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bioRxiv - Developmental Biology 2022Quote: ... The isolated proteins were then prepared for mass spectrometry using an in-solution protein digestion kit (Thermo Scientific). In brief ...
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bioRxiv - Molecular Biology 2021Quote: ... The total protein concentration was measured using a Microplate BCA Protein Assay Kit (Pierce™, Thermo Fisher Scientific). Proteins were separated on an SDS-PAGE gel and then transferred to a nitrocellulose (NC ...
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bioRxiv - Molecular Biology 2021Quote: ... The total protein concentration of the cleared lysates was determined using a BCA Protein-Assay (Thermo Scientific 23225) and 20 μg of protein was loaded per lane and further processed as described above.
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Characterization of pyruvate dehydrogenase complex E1 alpha and beta subunits of Mycoplasma synoviaebioRxiv - Microbiology 2019Quote: ... The purified protein products were quantified by using BCA Protein Assay Kit (Thermo Scientific-Pierce, Rockford, IL, USA) and analyzed by 10% SDS-PAGE with Coomassie blue staining.
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bioRxiv - Cancer Biology 2020Quote: ... Equal amounts of protein were loaded onto NuPAGE 4-12% Bis-Tris Protein Gels (Thermo Fisher Scientific, #NP0335) for separation by SDS-PAGE ...
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bioRxiv - Neuroscience 2019Quote: ... Samples were analyzed for protein content using the BCA Spec method (Thermo Scientific Pierce Protein Biology; Waltham, MA). Protein (25 µg ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Protein amount in each sample was measured using a BCA protein assay kit (Thermo Scientific, Waltham, MA, USA) following manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2019Quote: ... Protein concentration of the supernatant was determined using Micro BCA protein assay kit (Thermo Fisher Scientific, catalog #23235). The lysates were separated on a 12% SDS-PAGE or a NuPAGE™ 4-12% Bis-Tris protein gel ...
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bioRxiv - Molecular Biology 2019Quote: ... The sizes of proteins were estimated with a PageRuler™ Prestained Protein Ladder (10-180 kDa) (26616LCS, ThermoFisher). Protein quantifications were performed with Bio-Rad ChemiDoc Touch Imaging program.
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bioRxiv - Physiology 2019Quote: ... The concentration of protein was measured using the BCA Protein Assay kit (Thermo Fisher Scientific Cat. No# 23225). Subsequently ...
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bioRxiv - Neuroscience 2019Quote: ... The protein concentration of the protein extract supernatant was determined using a BCA assay kit (Thermo Fisher Scientific). pCRMP2 was immunoprecipitated from 2 mg of protein extracts by anti-phosho-T514 CRMP2 rabbit polyclonal antibody (1:100 dilution ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The concentrations of protein in homogenate samples were determined using Pierce BCA protein assay (Thermo Scientific, Rockford, IL).
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bioRxiv - Pathology 2020Quote: ... Protein and peptide concentrations were measured in duplicate using the Qubit® Protein Assay Kit (Invitrogen, Carlsbad, CA). Peptides were dried by vacuum centrifugation in 25 μg aliquots ...
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bioRxiv - Cancer Biology 2020Quote: ... The protein concentration was quantified with a Pierce bicinchoninic acid protein assay kit (Pierce; Thermo Fisher Scientific, Inc.). Subsequently ...
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bioRxiv - Immunology 2021Quote: ... The protein content of the viral lysate was quantified using BCA protein assay kit (Thermo Scientific, Waltham, MA), aliquoted and stored at -80°C.
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bioRxiv - Neuroscience 2020Quote: The protein aggregate fractions resuspended in 8M urea were tested using Pierce™ BCA Protein Assay Kit (ThermoFisher) for total protein quantitation.
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bioRxiv - Microbiology 2021Quote: Protein concentrations in the samples were determined using the Pierce 660 nm Protein Assay Reagent (Thermo Fisher Scientific) in 96-well plates by the absorbance in an Infinite M200 plate reader (TECAN ...
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bioRxiv - Bioengineering 2021Quote: EV-rich fractions (7-10) from SEC were initially determined by protein (Pierce™ BCA Protein Assay, ThermoFisher) and particle quantification using nanoparticle tracking analysis (NTA ...
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration of the purified EVs was determined by Qubit protein assay kit (Thermo Fisher Scientific Inc.).
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bioRxiv - Bioengineering 2021Quote: ... Total protein amount in each sample was determined for normalization using a Pierce BCA Protein Assay (ThermoFisher Scientific) following the recommended procedure from the manufacturer ...
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bioRxiv - Bioengineering 2021Quote: Tissue homogenates were thawed and protein concentration was measured using the Pierce 660 nm Protein Assay (ThermoFisher Scientific). 200 μg of tissue homogenate was alkylated with 40 mM iodoacetamide for 30 min at RT in the dark with end-over-end rotation ...
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bioRxiv - Biochemistry 2020Quote: ... The protein concentration of each fraction was measured using micro BCA protein assay kit (Thermo Fisher Scientific, MA) with VarioSkan Flash microplate reader (Thermo Fisher Scientific ...
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bioRxiv - Biophysics 2021Quote: ... The quality of each protein preparation was assessed by NuPAGE Novex 4–12% Bis-Tris protein gels (ThermoFisher). Gels were stained with Coomassie Brilliant Blue R-250 (ThermoFisher ...
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bioRxiv - Neuroscience 2020Quote: ... Supernatant was removed and a BCA protein assay (Pierce BCA Protein Assay Kit; Thermo Fisher Scientific; cat # 23227) was used to calculate protein concentration.
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bioRxiv - Neuroscience 2021Quote: ... Each sample’s total protein concentration was determined using Pierce BCA Protein Assay Kit (Thermo Fisher Scientific; Waltham, MA). ELISAs for CGRP (MyBioSource ...
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bioRxiv - Neuroscience 2021Quote: ... The soluble fraction was collected and the protein concentration was determined using Pierce 660nM Protein Assay Kit (ThermoFisher). The samples underwent SDS-polyacrylamide gel electrophoresis and were transferred on nitrocellulose membrane (BioRad ...