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Citations for Thermo Fisher :
4851 - 4900 of 10000+ citations for Recombinant Mouse Ager His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine ...
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bioRxiv - Bioengineering 2019Quote: ... Mouse-anti-His6 primary antibody and goat anti-mouse IgG H&L (Alexa Fluor® 488) were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Genetics 2019Quote: ... and incubated with 1:50 mouse anti-wg (4D4, DSHB) primary antibody and 1:500 anti-mouse 488 (A-21202, Molecular Probes) secondary antibody ...
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bioRxiv - Neuroscience 2019Quote: ... Slices were washed (5x 5 min with PBST) then incubated in secondary antibodies against mouse (1:500 goat anti-mouse, ThermoFisher A11001) and rabbit (1:500 goat anti-rabbit ...
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Intestinal Enteroid Monolayers Model the Human Intestinal Environment for Escherichia coli InfectionbioRxiv - Microbiology 2021Quote: ... Secondary AlexaFluor antibodies (488 Goat Anti-Mouse, cat. A11001, Life Technologies, 488 Goat Anti-Rabbit, cat. A32731, Invitrogen, 660 Goat Anti-Mouse, cat. A21055, Invitrogen, 660 Goat Anti-Rabbit ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were incubated with appropriate secondary antibodies (488 anti-chicken, 546 anti-mouse, 647 anti-guinea pig, 647 anti-rabbit, 647 anti-mouse, Alexa Fluor, Life Technologies)
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bioRxiv - Developmental Biology 2021Quote: ... The PAIso-seq1 libraries for mouse oocytes were constructed with total RNA prepared from mouse oocytes using TRIzol reagent (Life Technologies). The libraries sequenced using PacBio Sequel II instruments at Annoroad.
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bioRxiv - Microbiology 2020Quote: ... They were then washed 3 times with PBS and incubated with goat anti-mouse conjugated to Alexa Fluor 488-conjugated goat anti-mouse IgG antibody (Thermo Fisher) containing DAPI in immunofluorescence buffer for an additional incubation of 30 minutes ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were isolated from wildtype adult C57BL/6 mouse spleen and lymph nodes using the Dynabeads FlowComp Mouse Pan T (CD90.2) Kit (Thermo Fisher Scientific) as per the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2022Quote: The Tregs and the CD4+/CD25- T cells were isolated from C57BL/6 mouse spleen using the Mouse CD4+CD25+ Treg Cells Kit (Invitrogen 11463D). The CD4+/CD8+ cells were sorted from testis ...
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bioRxiv - Cancer Biology 2022Quote: ... TNF alpha mouse high sensitivity ELISA kit and IL-6 mouse high sensitivity ELISA kit were from Invitrogen (Thermo Fisher Scientific), mouse CCL2/JE/MCP-1 quantikine ELISA kit ...
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bioRxiv - Microbiology 2022Quote: ... Immunostaining of NS1 and TRIM25 was realized by using the following antibody combination: mouse anti-NS1/goat anti-mouse Alexa 488 (ThermoFisher Scientific) and rabbit anti-TRIM25 (abcam)/goat anti-rabbit Atto647N (sigma) ...
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bioRxiv - Microbiology 2022Quote: ... For determination of infection rates employing wide field fluorescence microscopy the following antibodies in combination with a DAPI counter stain were used: mouse anti-nucleoprotein/goat anti-mouse Alexa488 (ThermoFisher Scientific). Cells grown on cover slips were mounted on microscopy slides using Mowiol.
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bioRxiv - Molecular Biology 2023Quote: ... The anti-FLAG antibody was detected using an anti-mouse Alexa 647 secondary antibody (Alexa 488 goat anti-mouse, 1:500 dilution, RT, 1 h, Invitrogen, #A11029). The images were captured with an InCell2000 imager.
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bioRxiv - Neuroscience 2024Quote: ... OFC and mPFC sections were stained to visualize glial fibrillary acidic protein (GFAP) (primary antibody: mouse anti-GFAP antibody, Sigma-Aldrich; secondary antibody: donkey anti-mouse Alexa 594 antibody, ThermoFisher), and with fluorescent Nissl and DAPI ...
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bioRxiv - Plant Biology 2024Quote: ... goat anti-mouse Alexa Fluor Plus 488 and F(ab’)2-goat anti-mouse Alexa Fluor Plus 555 from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... phospho-tau (Ser202, Thr205) mouse monoclonal antibody (AT8, catalog #MN1020), and tau mouse monoclonal antibody (HT7, catalog #MN1000) from Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2024Quote: BV-2 cells were fixed with 4% PFA for 10 minutes and subsequently incubated with Mouse-on-Mouse IgG Blocking Solution (Invitrogen R37621) at room temperature for 30 min ...
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bioRxiv - Biophysics 2024Quote: ... blocked with 1% BSA and stained with primary Anti-mouse laminin α5 (gift from Lydia Sorokin) and secondary Anti-mouse IgG AF594 (Invitrogen, A11005). Between all steps ...
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bioRxiv - Molecular Biology 2023Quote: ... Western blotting was performed with 1:5000 dilution of mouse monoclonal anti-DBP5 and 1:10000 dilution of mouse monoclonal anti-GAPDH (Thermo Fisher) primary antibody overnight at 4°C and 1:10000 anti-mouse DyeLight 650 secondary to detect protein.
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bioRxiv - Molecular Biology 2023Quote: ... blocked with 1 %BSA/ 1%DMSO/PBS for 1 hr at room temperature and immunostained with mouse anti-SV2 AB2315387 and secondary Alexa Fluor 555 anti-mouse antibody from Molecular probes, Eugene.
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bioRxiv - Physiology 2023Quote: ... or SC71-S (1:25, Mouse IgG1, DSHB) followed by Alexa Fluor 488 or 647 (1:500, Goat anti-mouse, ThermoFisher Scientific). Images were acquired using a confocal microscope (Zeiss Axiovert 200 ...
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bioRxiv - Cell Biology 2023Quote: Mouse embryonic fibroblasts (MEFs) were isolated from E13.5 mouse embryos and grown in ‘MEF medium’ containing DMEM (41966029, Thermo Fisher Scientific) supplemented with 10% FBS (10270106 ...
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bioRxiv - Molecular Biology 2023Quote: ... embryos were incubated for 2h at RT with Alexa Fluor 488 nm anti-mouse or Alexa Fluor 568 nm anti-mouse and Alexa Fluor 647 nm anti-goat secondary antibodies (Life Technologies) along with DAPI counterstaining (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... berghei infected HepG2 cells were immunolabeled using anti-PbHSP70 (1:200, 2E6 mouse mAb) (62)to mark EEFs followed by donkey anti-mouse Alexafluor488 (Invitrogen A21202). MSP1 was immunolabeled with Anti-PBANKA_083100 ...
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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bioRxiv - Cancer Biology 2024Quote: ... and HRP-conjugated rabbit anti-mouse secondary antibody (#31430) and HRP conjugated Goat anti-mouse IgM (#31440) for immunoblotting were from Thermo Fisher. APC conjugated anti-human ganglioside GD2 antibody (#357306 ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA, Invitrogen, respectively). Serum noradrenaline was extracted with the use of a cis-diol–specific affinity gel ...
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bioRxiv - Cell Biology 2019Quote: ... and subjected to immunoprecipitations with goat anti-GFP antibodies (generic goat IgGs as control) or mouse anti-γ-adaptin antibodies (mouse hybridoma C7 antibodies as control) bound to protein G-Sepharose beads (Fisher Scientific). Samples were rotated end-over-end at 4°C for 2 h and washed 2 x in buffer D+ and 1 x in PBS ...
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bioRxiv - Genetics 2021Quote: ... incubated with mouse anti-HA antibody and then with the secondary antibody conjugated with Donkey-anti-mouse Alexa 567 (Invitrogen Molecular Probes). DNA was stained with 0.5 mg/ml DAPI (Sigma-Aldrich) ...
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bioRxiv - Molecular Biology 2019Quote: ... quickly washed with PBS+ before incubating with 70 µl of secondary antibody mix (Goat anti-mouse Alexa Fluor® 488 or Goat anti-mouse Alexa Fluor® 594 1/400 in PBS+, Invitrogen) for 1 h at RT in a moist chamber ...
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bioRxiv - Developmental Biology 2020Quote: ... Pros (mouse 1:100, DSHB MR1A), Hairless (guinea pig 1:500, Annett and Dieter) and V5 (mouse 1:500, Invitrogen R960-25).
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bioRxiv - Neuroscience 2019Quote: ... 1:200) follwed by Alexa Fluor 488 anti-mouse IgG or Alexa Fluor 568 anti-mouse IgG (A11001 and A11004, Thermo Fisher Scientific) as the secondary antibodies (1:1000) ...
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bioRxiv - Neuroscience 2020Quote: ... goat anti-mouse IgG1 Alexa Fluor 568, A-21124, or with goat anti-mouse IgG2a Alexa Fluor 488, A-21131, Thermo Fisher Sci) at room temperature (23 °C ...
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bioRxiv - Physiology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Neutrophils were labelled with 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) 15 min prior to recordings ...
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bioRxiv - Microbiology 2021Quote: ... 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G ...
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bioRxiv - Microbiology 2021Quote: ... the murine vasculature was labelled with 15 μg Dylight488-conjugated anti-mouse CD31 (LEAF purified anti-mouse CD31, clone 390, Biolegend, #102412 + DyLight488 Antibody Labelling Kit, ThermoFisher Scientific, #53024) and the human vasculature with 100μg of Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Microbiology 2021Quote: ... and 2.5 μg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530) were injected to label and visualize the human vessels ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452; 1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Cancer Biology 2022Quote: ... Mouse cytokines were measured using a Cytokine & Chemokine Convenience 26-Plex Mouse ProcartaPlex™ Panel 1 (Invitrogen, EPXR260-26088-90, Carlsbad, CA) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine, Invitrogen, T2762) for 45 min at 37°C ...
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bioRxiv - Developmental Biology 2020Quote: ... an anti-Flag antibody or normal mouse IgG were added to the nuclear extract together with Dynabeads bearing anti-Mouse IgG (Thermo Fisher Scientific). After incubation for 1 h with gradual rotation at 4°C ...
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bioRxiv - Immunology 2020Quote: ... functional grade anti-mouse IL-4 (eBioscience™, clone: 11B11), functional grade anti-mouse INF-γ (eBioscience™, clone: XMG1.2) were from ThermoFisher, Loughborough ...
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bioRxiv - Immunology 2021Quote: The total IgG concentration in mouse serum was determined using the IgG (Total) Mouse Uncoated ELISA Kit (Invitrogen, Cat no. 88-50400) according to the manufacturer’s instructions ...
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bioRxiv - Biophysics 2022Quote: ... coverslips were incubated for 2 hours in a 1:1000 dilution of secondary antibody (anti-rabbit Alexa 647, Invitrogen, A-27040; anti-mouse Alexa 647, Invitrogen, A-32728, anti-mouse Alexa 488, Invitrogen, A-32723). Coverslips were washed 3 times with blocking buffer ...
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bioRxiv - Neuroscience 2022Quote: ... Cells were incubated overnight at 4 °C with primary antibodies — targeting α-syn (Mouse anti α-syn, AA 124-134, ab1903, Abcam; Mouse anti α-syn 4B12, AA 103-108, IgG1, MA1-90346, ThermoFisher; pericytes (Rabbit anti-PDGFR β ...
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bioRxiv - Neuroscience 2022Quote: ... donkey anti-mouse IgG, 1:500, 715-165-150, Jackson ImmunoResearch; AF488: donkey anti-mouse IgG, 1:500, A-21202, Life Technologies).
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bioRxiv - Synthetic Biology 2024Quote: ... as previously described [63] and neutrophils were labelled by were labeled with 2.5 µg Dylight550-conjugated anti-mouse Ly-6G (LEAF purified anti-mouse Ly-6G, clone 1A8, Biolegend, #127620 + DyLight550 Antibody Labelling Kit, ThermoFisher Scientific, #84530). Mice were infected by intravenous injection of 100 µl of the bacterial inoculum (107 CFU total ...