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Citations for Thermo Fisher :
4851 - 4900 of 7736 citations for Recombinant Human C1QB His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: A serial dilution from 1 ng to 1,000 ng Human Liver Total RNA (ThermoFisher) was prepared to generate the various input samples ...
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bioRxiv - Bioengineering 2023Quote: ... were coated with polyclonal anti-human EPO (4 mg/mL; Thermo Fisher Scientific, USA) in 0.1 M sodium bicarbonate buffer (pH 8.3 ...
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bioRxiv - Physiology 2022Quote: Huh7 and HepG2 human hepatoma cells were maintained in in DMEM (Thermofisher Cat# 11965092) supplemented with 10% fetal bovine serum ...
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bioRxiv - Bioengineering 2023Quote: Hep3B human hepatoma cell line (ATCC) was cultured in RPMI 1640 (Gibco, 11875-093) supplemented with 10% fetal bovine serum (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... and an Alexa Fluor 594–conjugated goat anti-human secondary antibody (ThermoFisher, A-110014). Confocal images were collected as 31 z-stacks at 0.5 μm intervals to visualize the entire meiotic spindle ...
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bioRxiv - Bioengineering 2023Quote: ... purified pan T cells were cultured with human anti-CD3/anti-CD28 dynabeads (Gibco) at a 2:1 ratio in the complete T cell media supplementing with 50μM 2-mercaptoethanol and 100 UꞏmL−1 of IL-2 upto a week.
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bioRxiv - Bioengineering 2023Quote: ... Undiluted sample buffer was assayed for ghrelin content using ghrelin human ELISA kit (ThermoFisher) according to manufacturer specifications.
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bioRxiv - Biochemistry 2022Quote: The human embryonic kidney HEK293 cells were cultured in Dulbecco’s modified Eagle’s medium (Gibco) supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin solution at 37 °C under 5% CO2 ...
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bioRxiv - Cell Biology 2022Quote: Human fibroblasts were seeded and transfected with eEF2 silencing RNA (si-eEF2) (#4392420, Ambion) to detect the potential role of eEF2 in tendon healing ...
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bioRxiv - Biophysics 2023Quote: The gene encoding human A2AAR (1-316) was cloned into a pPIC9K vector (Invitrogen) at the BamHI and NotI restriction sites ...
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bioRxiv - Cancer Biology 2023Quote: ... Human bone marrow stromal cell line HS5 was maintained in DMEM (Gibco, Carlsbad, USA). AML cell lines U937 (FLT3-negative ...
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bioRxiv - Bioengineering 2023Quote: ... and the resulting supernatant was assayed with human albumin ELISA kit (EHLAB, ThermoFisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... These were incubated with a Human Gene 2.0 ST Array (Affymetrix/Thermo Fisher Scientific) for 16 hours ...
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bioRxiv - Immunology 2023Quote: ... These were incubated with a Human Gene 2.0 ST Array (Affymetrix/Thermo Fisher Scientific) for 16 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-human/mouse Foxp3 Ab pre-labeled with Alexa Fluor 555 (Molecular Probes, A20181), Alexa Fluor® 647-conjugated anti-mouse integrin αvβ5 Ab ...
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bioRxiv - Immunology 2023Quote: ... a secondary antibody goat anti-Human IgG Alexa Fluor 568 (Thermo Fisher, A-21090) was added using a dilution factor of 1:2000 and incubated 30 min at RT ...
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bioRxiv - Immunology 2023Quote: ... T cell proliferation was stimulated with Human T-Activator CD3/CD28 Dynabeads (ThermoFisher, #11161D). Cells were cultured in RPMI1640 supplemented with 10% FBS ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... and human Gγ2 were generated with the Bac-to-BacTM baculovirus expression system (Invitrogen). Spodoptera frugiperda Sf9 cells (Invitrogen ...
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bioRxiv - Biophysics 2023Quote: ... Human MCM8_61-840 and MCM9_1-684 were inserted into the pFastbac-Dual vector (Invitrogen). Gallus gallus MCM8_50-830 and MCM9_1-691 were inserted into the pFastbac-1 vector ...
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bioRxiv - Neuroscience 2024Quote: ... Human iHEK-Tau cells were cultured in a complete medium using DMEM (Gibco, #11965), supplemented with 10% heat-inactivated fetal bovine serum (HI-FBS ...
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bioRxiv - Microbiology 2023Quote: Human ileocecal adenocarcinoma (HCT-8) cells (ATCC) were cultured in RPMI 1640 medium (Invitrogen) supplemented with 10% heat-inactivated fetal bovine serum (Sigma-Aldrich) ...
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bioRxiv - Biochemistry 2023Quote: Human Flvcr2 was knocked down in HBEC5i cells using siRNA (Silencer Select siRNA; Ambion). Cells were transfected with either scrambled siRNA or Flvcr2 siRNA using Lipofectamine 3000 (Fisher Scientific) ...
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Epigenetic deregulation of IFN and WNT pathways in AT2 cells impairs alveolar regeneration (in COPD)bioRxiv - Cell Biology 2023Quote: ... PDPN and CD45 antibodies (Anti-human CD326 (EpCAM-PE, 12-9326-42, Affymetrix eBioscience), Anti-human podoplanin (PDPN)-Alexa Fluor 647 (337007 ...
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bioRxiv - Immunology 2023Quote: ... anti-human FcεRI (0.5 µg/mL, AER-37, eBioscience/Thermo Fisher, Waltham, MA, USA), and appropriate isotype controls ...
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bioRxiv - Biochemistry 2024Quote: ... Human ZIPK-siRNA (am-4390824) and scrambled siRNA (am-AM4613) were purchased from Ambion/Thermofisher Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... Human Embryonic Kidney (HEK-293) cells were transfected with Lipofectamine 2000 (Invitrogen 11668-019) and 16 μg DNA per vector (light and heavy chains) ...
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bioRxiv - Genomics 2024Quote: ... the CECs were pelleted and resuspended in stabilisation media (Human Endothelial-SFM (Life Technologies) supplemented with 5% foetal bovine serum (FBS) ...
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bioRxiv - Systems Biology 2023Quote: ... and stimulated with anti-human CD3/CD28 CTS Dynabeads (ThermoFisher Scientific, catalog no. 40203D) at a 1:1 cell:bead ratio ...
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bioRxiv - Molecular Biology 2023Quote: IMR-90 human lung fibroblasts (Coriell Institute) were cultured in high-glucose DMEM (Gibco) supplemented with 10% heat-inactivated FBS (Gibco) ...
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bioRxiv - Physiology 2024Quote: ... and C3 (rabbit anti human, 1:100, cat. no.: PA5-21349, Thermo Fisher Scientific). Thereafter ...
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bioRxiv - Developmental Biology 2024Quote: ... KLF2 was evaluated in human cells with the probe Hs07291763_gH (ThermoFisher Scientific, Cat.#: 4331182) and in zebrafish with klf2a ...
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bioRxiv - Microbiology 2024Quote: ... Human foreskin fibroblast (HFF-1) cells (ATCC SCRC-1041) were maintained in DMEM (Gibco) with 20% FBS ...
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bioRxiv - Microbiology 2024Quote: Human Foreskin Fibroblasts (HFFs) were maintained in Dulbecco’s minimal essential medium (DMEM; Fisher Scientific) and BS-C-1 cells (ATCC CCL-26 ...
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bioRxiv - Developmental Biology 2024Quote: ... Human iPSCs were cultured in feeder-free conditions on GelTrex-coated plates (ThermoFisher Scientific) in mTeSR-1 media (Stem Cell Technologies ...
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bioRxiv - Neuroscience 2024Quote: The human DBT cDNA plasmid was obtained from the Ultimate ORF collection (Thermo Fisher). Synonymous mutations in the DBT cDNA were generated with Q5-site direct mutagenesis (NEB ...
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bioRxiv - Cell Biology 2024Quote: ... Alexa Fluor 488- and Alexa Fluor 594-conjugated human transferrin were obtained from Invitrogen. Brefeldin A (BFA) ...
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bioRxiv - Neuroscience 2024Quote: ... Samples were quantified by an ELISA kit for total human tau (ThermoFisher Cat# KHB0041) as per manufacturer’s protocol to determine brain ISF tau levels before ...
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bioRxiv - Molecular Biology 2024Quote: ... RRID:CVCL_0291) and Flp-In-T-REx-293 (human, female, embryonic kidney, epithelial; Thermo Fisher Scientific ...
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bioRxiv - Bioengineering 2024Quote: ... were cultured as standard in Human Large Vessel Endothelial Cell Basal Medium (Gibco, M200500) supplemented with low serum growth supplement (Gibco ...
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bioRxiv - Systems Biology 2024Quote: ... The resulting suspension of human tumor cells was adjusted in DMEM medium (Gibco, #11965084) to a concentration appropriate for loading into a 10X genomics Chromium controller (3’ gene expression kit ...
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bioRxiv - Immunology 2024Quote: ... Isolated T cells were immediately activated with DynaBeads Human T-Activator CD3/CD28 (ThermoFisher), using a 1:1 bead:cell ratio ...
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bioRxiv - Physiology 2024Quote: ... Human THP-1 monocytes were cultured in RPMI 1640 medium (Gibco, cat no. 11875093) supplemented with 10% FCS ...
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bioRxiv - Immunology 2024Quote: ... an Alexa Fluor 647-labeled goat anti-human IgG [H+L] secondary antibody (Invitrogen) was used to detect bound IgG1 to the bacterial surface ...
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bioRxiv - Cell Biology 2020Quote: ... Clarified lysates were then incubated with 20 μg of recombinant glutathione S-transferase (GST)-RBD-MICAL-L1 and Pierce™ Glutathione magnetic agarose beads (Life Technologies-Invitrogen, cat# 78601) for 2 hrs at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were transfected to HEK293 cells (ATCC® CRL-1573™) and each recombinant protein was purified using HisPur™ Ni-NTA column (Invitrogen, Cat# 88226). The specificity of recombinant protein was further validated by SDS-PAGE and Western blot evaluations.
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bioRxiv - Immunology 2024Quote: All CoMiX were generated from HEK293T cells transfected with four μg of recombinant plasmid DNA using Lipofectamine-3000 (Invitrogen, Thermo Fisher Scientific BVBA, Merelbeke, Belgium) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: The VHH M13 phage-display library was subjected to two rounds of affinity enrichment (panning) against immobilized recombinant OspA on Nunc-Immunotubes™ (Thermo Fisher Scientific, Waltham, MA) using protocols described elsewhere (56) ...
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bioRxiv - Biochemistry 2023Quote: ... and the bound target-Fab complexes were incubated with an HRP-conjugated Pierce recombinant protein L (cat: 32420, Thermofisher, 1:5000 dilution in ELISA buffer) for 30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... this latter was performed on 1 ng of genomic DNA (gDNA) of each organism listen in Table 2 by using the Taq DNA polymerase recombinant (Invitrogen™, Carlsbad, 254 CA, USA) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... with formulations calling for L-WRN conditioned medium instead using 500 pM Gibco™ Wnt Surrogate-Fc Fusion Recombinant Protein (Thermo Fisher Scientific, cat# PHG0402), 500 ng/mL recombinant RSPO1 (Thermo Fisher Scientific ...