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Citations for Thermo Fisher :
4851 - 4900 of 5129 citations for PLXDC1 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... cells were washed with 5 mL of PBS and incubated with a blocking buffer comprised of 1% bovine serum albumin (BSA, Fisher Scientific cat. no. BP9703) and 10% goat serum (Sigma Aldrich cat ...
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bioRxiv - Cell Biology 2022Quote: ... then room temperature blocking solution (3% BSA in D-PBS) containing secondary antibody (IgG2b Cross-Adsorbed Goat anti-Mouse, Alexa Fluor™ 555 [Invitrogen, 10412832], 1:2000) was added using Dragonfly Discovery liquid dispenser and incubated for 1 h at room temperature ...
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bioRxiv - Plant Biology 2022Quote: ... They were then treated with secondary antibody solution (1 : 200 of Alexa Fluor 488 goat anti-rabbit IgG; Molecular Probes, in the blocking buffer) for 2 hours at 37 °C and washed three times ...
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bioRxiv - Microbiology 2022Quote: ... the coverslips were washed three times in PBS with 0.1% saponin and incubated for one hour at room temperature in blocking solution with 1.6 μg/ml DAPI dilactate (Molecular Probes, Thermo Fisher Scientific, Waltham, USA), 100 μg/ml Concanavalin A-Texas red conjugate (Invitrogen ...
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bioRxiv - Neuroscience 2024Quote: ... Slice were then incubated overnight at 4°C in blocking solution containing a rabbit anti-Parvalbumin (PV) antibody (1:400, cat # PA1933, Thermo Fisher Scientific, Waltham, MA). Slices were then washed and incubated blocking solution containing a secondary antibody donkey anti-rabbit Alexa568 diluted to 1:1000 (Abcam ...
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bioRxiv - Neuroscience 2024Quote: ... sections were incubated for 2 h in blocking/permeabilization buffer with goat anti-rabbit AlexaFluor 488 and goat anti-chicken AlexaFluor 568 (Invitrogen, Carlsbad, CA; 1:500). After washing ...
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bioRxiv - Synthetic Biology 2023Quote: ... cells were incubated overnight at 4°C in blocking buffer with the antibodies anti-HA tag conjugated to DyLight 550 (ThermoFisher Scientific, catalog #26183-D550) and anti-TIM23 (Abcam ...
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LRP1 mediates leptin transport by coupling with the short-form leptin receptor in the choroid plexusbioRxiv - Neuroscience 2023Quote: ... and then incubated in the same blocking solution containing secondary antibody (Donkey anti-rabbit Alexa fluorophore 647, 1:500 Thermo Fisher Scientific, Waltham, MA) for 1 hour at RT ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were then washed 3 times with PBS and then blocked for 30 minutes at room temperature with antibody blocking buffer (150 mM NaCl, 50 mM Tris-Base, 1% BSA, 100 mM L-lysine 50% normal goat serum (Thermo Fisher, Cat# 01-6201), 0.2% Triton (Roche ...
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bioRxiv - Biophysics 2023Quote: ... Secondary antibody staining was performed for 1 hour at RT in blocking solution (AF647 anti-rabbit #A21244, Thermo Fisher Scientific, 1:1.000; AF488 Phalloidin #A12379, Thermo Fisher Scientific, 1:1.000). Cells were washed trice for 10 min before mounting the coverslips on Marienfeld Superior Microscope slides with molds (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: Mouse peripheral blood was processed to remove RBC via lysis prior to Fc blocking and then stained with antibodies against surface markers including biotin-CD93 (Invitrogen, cat # 13-5892-85), BV605-CD19 (BD ...
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bioRxiv - Developmental Biology 2023Quote: ... in 1x PBS and blocked for 1 hour (h) in blocking buffer made of washing buffer and 1% heat-inactivated goat serum (HIGS) (Life Technologies, Waltham, MA, USA). Skin sections were fixed in 4% PFA for 10 min before they were washed and blocked in blocking buffer containing 10% HIGS ...
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bioRxiv - Neuroscience 2023Quote: Slices were stained for green fluorescent protein (GFP) with a rabbit monoclonal anti-GFP antibody (G10362, Molecular Probes, 1:1000 dilution in blocking buffer) and for tyrosine hydroxylase (TH ...
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bioRxiv - Neuroscience 2023Quote: ... sections were blocked with Mouse-on-Mouse IgG Blocking Solution for 1 h at room temperature (1:30; Thermo Fisher Scientific, Catalog no. R37621). Sections were incubated overnight at 4°C with primary antibodies—rabbit anti-Iba1 (1:1000 ...
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bioRxiv - Immunology 2024Quote: ... Samples were washed in PBS-Tx and blocked for 1 hour in blocking buffer and stained with secondary antibody (Alexa Fluo-647, SA5-35500 Thermo Fisher Scientific, 1:500) for 2 hours ...
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bioRxiv - Microbiology 2020Quote: ... The digestion was stopped with 4% formic acid (FA) (#94318, Fluka) and digested peptides were purified with C18 Spin Columns Pierce™ (#89870, ThermoFisher Scientific, USA). Peptides were eluted with 2 x 80% Acetonitrile (ACN ...
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bioRxiv - Cell Biology 2020Quote: ... The eluted peptide samples were analyzed by a Q Exactive Plus™ hybrid quadrupole-Orbitrap™ mass spectrometer (Catalog No. IQLAAEGAAPFALGMAZR, ThermoFisher Scientific). The electrospray voltage was set to 2.0 kV ...
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bioRxiv - Cell Biology 2020Quote: Each TMT labeled peptide set was fractionated to improve depth of proteome coverage using a Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific, Part 84868). The fractionation was performed according to the manufacturer’s protocol and yielded 8 peptide fractions for analysis ...
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bioRxiv - Cell Biology 2020Quote: Nanoflow LC-MS analysis of tryptic peptides was performed using a quadrupole Orbitrap mass spectrometer (Q Exactive HF-X, Thermo Fisher Scientific 17) connected to an EASYnLC 1200 ultra-high-pressure system (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2021Quote: ... The samples were subjected to tryptic digestion and the resulting peptide mixtures were analyzed by nano-ESI-LC-MS/MS (Thermo Scientific LTQ Orbitrap). Proteins were identified using Mascot (Matrix Science ...
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bioRxiv - Biochemistry 2020Quote: ... and ~200 ng of peptides were injected for each chromatographic run using a Dionex UltiMate 3000 uHPLC system (Thermo Fisher Scientific, Bremen, Germany). Solvent A was 1% CH3CN in 0.1% (v/v ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting peptides were desalted and analyzed by LC-MS/MS on a Q-Exactive plus mass spectrometer (Thermo-Fisher scientific, Pittsburgh, PA) fitted with a capillary HPLC (easy nLC 1000 ...
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bioRxiv - Biochemistry 2022Quote: ... Labeled peptides were first trapped on an Acclaim™ PepMap™ 100 C18 precolumn (5 μM, 0.3 mm X 5 mm, Thermo Fisher Scientific) and eluted to the analytical column nanoEase M/Z Peptide BEH C18 Column (130Å ...
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bioRxiv - Neuroscience 2021Quote: ... Mass-spectrometry-based quantification of CaMKIIα/β-derived peptides was carried out by selected reaction monitoring (SRM) analysis using a TSQ Quantiva triple-stage quadrupole mass spectrometer (Thermo Fisher Scientific, U.S.A.). The following parameters were selected ...
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bioRxiv - Molecular Biology 2021Quote: ... One microgram peptides for global proteomics or all the enriched phosphorylated peptides were loaded and concentrated on an Acclaim PepMap 100 C18 precolumn (75 μm x 2 cm, Thermo Scientific, Waltham, MA) and then separated on an Acclaim PepMap RSLC column (75 μm x 25 cm ...
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bioRxiv - Cancer Biology 2020Quote: The PulseDIA acquisition of HeLa peptides was performed on a nanoflow EASY-nLC™ 1200 System (Thermo Fisher Scientific™, San Jose, USA) coupled to a Q Exactive HF-X hybrid Quadrupole-Orbitrap (Thermo Fisher Scientific™ ...
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bioRxiv - Molecular Biology 2019Quote: ... Eluted peptides were analyzed on a Q-Exactive Plus mass spectrometer or an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany). The software Scaffold 4.7.2 (Proteome Software Inc. ...
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bioRxiv - Biochemistry 2019Quote: ... The digested peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany) and stored at −80 °C.
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bioRxiv - Cancer Biology 2019Quote: ... The collected peptide was desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany).
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bioRxiv - Microbiology 2021Quote: ... NanoUPLC-hr MS / MS analysis of the resulting peptide mixtures were performed on a Q-Exactive orbitrap mass spectrometer (Thermo Fisher Scientific, USA), coupled with a nanoUltimate300 UHPLC system (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2020Quote: ... Tagged tryptic peptides (~250 μg) were subjected to strong cation exchange fractionation using a hand-held ICAT® Cartridge-cation-exchange system (Applied Biosystems, USA). Peptides were eluted using a gradient 30 ...
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bioRxiv - Microbiology 2020Quote: 50µg of peptides from each sample were labeled with isobaric tandem-mass-tag (TMT) 11 plex reagents (Thermo Fisher Scientific, San Jose, CA) in 20 mM pH 8.5 HEPES with 30% acetonitrile (v/v ...
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bioRxiv - Microbiology 2020Quote: The flow-through fraction of each TMT11 experiment was fractionated into 13 fractions using the Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific) according to manufacturer’s instructions.
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bioRxiv - Bioengineering 2021Quote: ... the UV absorbance of the NPs was measured at 205 nm before and after peptide coupling using a spectrophotometer (Nanodrop 2000, Thermo Fisher Scientific, UK) and the difference of the absorbance (ΔA205 ...
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bioRxiv - Molecular Biology 2020Quote: ... The separated peptides were then electrosprayed in the positive ion mode into an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher, San Jose, CA, USA) operated in data-dependent acquisition mode (3 s cycle time) ...
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bioRxiv - Biochemistry 2020Quote: ... and with or without CLASPIN pS945 peptide (MEELLNLC(pS)GKFTSQ) (Peptide Protein Research Ltd, Bishops Waltham, UK) at different concentrations in a Thermoscientific 96 well UV microplate (Fisher scientific, Loughborough, UK). The plates were incubated at 25 °C and absorbance at 340 nm was measured in a POLARstar Omega multimode microplate reader (BMG Labtech GmbH ...
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bioRxiv - Biochemistry 2020Quote: ... The peptide samples of EMARS products prepared as well as mouse samples were injected into an Ultimate 3000 RSLCnano (Thermo Fisher Scientific, MA). Mass analysis was performed on a LTQ Orbitrap XL mass spectrometer equipped with a nano-ESI source (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2021Quote: ... operated in the cone-jet spraying regime for efficient ion generation.33 Peptide ions were detected using a quadrupole orbitrap mass spectrometer (Q Exactive Plus, Thermo Scientific, Waltham, MA) between m/z 350–1,800 at 35,000 FWHM resolution (128 ms transient ...
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bioRxiv - Neuroscience 2021Quote: Eluted peptides were ionized by a nanospray source and detected by an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific, Waltham, MA, USA) operating in a data dependent mode (DDA) ...
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bioRxiv - Cancer Biology 2020Quote: ... Tagged tryptic peptides (∼250 μg) were subjected to strong cation exchange fractionation using a hand-held ICAT® Cartridge-cation-exchange system (Applied Biosystems, USA).
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm, C18, 3 µm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid ...
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bioRxiv - Plant Biology 2020Quote: The coding DNA sequence for human Factor IX without the native signal peptide was synthesized as N-terminal translational fusion to the signal-peptide coding sequence of PpAP1 (Pp3c5_19520V3.1)42 by GeneArt (Thermo Fisher Scientific, Waltham, MA, USA). It was cloned into the pAct5-MFHR1 plasmid41 to generate the final FIX expression vector ...
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bioRxiv - Systems Biology 2020Quote: ... fractionation for diGly peptide and proteome spectral libraries were performed on an UFLC system (Shimadzu) and EASY-nLC 1000 (Thermo Fisher Scientific, Germany), respectively.
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bioRxiv - Systems Biology 2021Quote: The concatenated and TMT-labelled peptide mixtures were analyzed using nanoflow liquid chromatography (LC-MS/MS) on an EASY nano-LC 1200™ system (Thermo Fisher scientific), connected to a Q Exactive HF (Thermo Fisher scientific ...
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bioRxiv - Systems Biology 2021Quote: ... Air dried peptides were analyzed using a nanoflow liquid chromatography (LC-MS/MS) on an EASY nano-LC 1200™ system (Thermo Fisher scientific), connected to a Q Exactive HF (Thermo Fisher scientific ...
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bioRxiv - Microbiology 2020Quote: Liquid chromatography coupled to mass spectrometry (LC-MS/MS) analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1000 (Thermo Fisher Scientific) ...
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bioRxiv - Neuroscience 2020Quote: ... The soluble fraction was then added to a new tube and the peptides were extracted and desalted using C18 desalting columns (Thermo Scientific, PI-87782). Peptides were quantified using BCA assay and a total of 1 ug of peptides were injected for LC-MS analysis.
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bioRxiv - Microbiology 2021Quote: ... and then tryptic peptides were subjected to LC-MS/MS on an Easy-Nlc 1000 coupled with Q Exactive Orbitrap instrument (Thermo Fisher Scientific, USA) under standard conditions ...
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bioRxiv - Biochemistry 2021Quote: ... The resulting peptides were then labelled with TMT ten-plex reagents according to the manufacturer’s protocol (Thermo Fisher Scientific, Loughborough, LE11 5RG, UK) and the labelled samples pooled and desalted using a SepPak cartridge according to the manufacturer’s instructions (Waters ...
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bioRxiv - Microbiology 2021Quote: ... isotopologue patterns of labeled peptides were extracted manually from mass spectral data using the Xcalibur Qual Browser (v3.0.63, Thermo Fisher Scientific, Waltham, MA, USA), and 13C incorporation was calculated as previously described24.